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The map label for this gene is aprF [H]

Identifier: 119357543

GI number: 119357543

Start: 1972472

End: 1973812

Strand: Reverse

Name: aprF [H]

Synonym: Cpha266_1746

Alternate gene names: 119357543

Gene position: 1973812-1972472 (Counterclockwise)

Preceding gene: 119357544

Following gene: 119357542

Centisome position: 62.98

GC content: 49.52

Gene sequence:

>1341_bases
ATGAAGAGTTTTTGGTATGTAATTCTTATAGCAGGCAGTTTTGTTTCGCCGCCTCTTTATGCCGCGCCAGTCACTATATC
GGAAGCTTATCTGAAAGCCCGAGATCATGATGCCCTGCTGGGTGCGGCAAAGGCTGATAATCTGGTGTATAAAGAGGAGG
TCGGCAAGGCGAGGGCCGGTTTACGCCCCAGTGTACGTCTGAACGCCTCACGCGGCCGCAACGGCACACAGCATGGTTAC
ATGGGACTATATGAGGCGCCTGATTTTTATAATACGGTCGTCTATGGTGTGACGCTTCGTCAACCATTGATTAATCTGTC
GAATATAGCTGAATACCAGCAGGCGAAAGCTGTGGCGGCAAAAAGTGATGCTGAATTGCGGAAAGAAGAGGCTGATCTGA
TTGTCAGACTTGCCGAGACCTATTGTAACGCATTGTATGCCGAGGATAACCTTACCTTCAGTCAGGCTCATATCAAGGCT
TCGGAAGAACAGCTTCAGCAGGCGAAACGACGGTTTGAAAAAGGGTTCGGAACCATTACCGAAATAAATGAAGCGCAGGC
TGACCTCGATATGGCGCTTGCTGAGGGACTTGAAATCGTCAACAGCGTCGAGTTCAGCCGCCGTGAGCTGGAGCATCTTA
TCGGAACGTATCCTGATGAACTCTGCAAGTTGGCGCCTGAAAAACTTGTGTTGGCCCGACCGACGCCAGGTCGTGTGGAA
TCTTGGATTGATCGAGCTCATGGAGAGAGTCCTCGAAGCTCGGCAGCGCACCTTGAGATGCAGATCGCAAAAAAAGAGAT
CGAAAAACAGAAGGCAAGCCGTTATCCGACGATTGATCTTGTTGCCGGGAGAAGCTATTCCGAGAGTGAAAATAATTATT
CAATCGGCTCAACCTATGAAACCTATTCGATCAGTCTGCAAATGAGCATGCCGATTTATACCGGTGGTTATGCCAGTGCA
TCGATCCGGCAGGCAAAAGCAAAGTGGCTTAAAGCGGGTGAGCAGTTTTTCTGGCAGGAACGGAGCATCGAATCTGAAGT
ACGCAAGTACTATAATACGGTTATCAGTACGATCGCACAGATCCAGGCCTATGAACAGGCGGTCAAATCCCGGGAAATTG
CTCTCGACGGCACAAAAAAAGGGTTTGGTGCTGGTCTGCGCAGTAATGTGGATGTTCTTGACGCTCTTCAGAATCTTCTT
GCCGCCAGACGTAATCTTGCGAAGTCGCGATACCAGTATATTCTTGCCCGTCTTTCGCTCAAGCAGACCGCAGGAGCATT
GTCGCCCGCTGATATTGAGGAGATCAATGGCTGGTTTGCAACGGCAAAAACAGCGAAATAG

Upstream 100 bases:

>100_bases
AGTCTCCATGAAGGAGGAGTGAGGGTATCTTCATGAATTTCTTTTTTTGAAAAAGGTCGCTCGAAAGAGCTGTTAACGAA
ACGCGAAGTCTGTATTGAAC

Downstream 100 bases:

>100_bases
ATAGCTGCTTTTTGCATTGAAACGTATAGCAGTTATTTCTTGTTGTTCTTCTTTAATAGCTAAAGTGCTTTGTATGATTT
TTTTTCTGGTTTTCGAAACA

Product: TolC family type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKSFWYVILIAGSFVSPPLYAAPVTISEAYLKARDHDALLGAAKADNLVYKEEVGKARAGLRPSVRLNASRGRNGTQHGY
MGLYEAPDFYNTVVYGVTLRQPLINLSNIAEYQQAKAVAAKSDAELRKEEADLIVRLAETYCNALYAEDNLTFSQAHIKA
SEEQLQQAKRRFEKGFGTITEINEAQADLDMALAEGLEIVNSVEFSRRELEHLIGTYPDELCKLAPEKLVLARPTPGRVE
SWIDRAHGESPRSSAAHLEMQIAKKEIEKQKASRYPTIDLVAGRSYSESENNYSIGSTYETYSISLQMSMPIYTGGYASA
SIRQAKAKWLKAGEQFFWQERSIESEVRKYYNTVISTIAQIQAYEQAVKSREIALDGTKKGFGAGLRSNVDVLDALQNLL
AARRNLAKSRYQYILARLSLKQTAGALSPADIEEINGWFATAKTAK

Sequences:

>Translated_446_residues
MKSFWYVILIAGSFVSPPLYAAPVTISEAYLKARDHDALLGAAKADNLVYKEEVGKARAGLRPSVRLNASRGRNGTQHGY
MGLYEAPDFYNTVVYGVTLRQPLINLSNIAEYQQAKAVAAKSDAELRKEEADLIVRLAETYCNALYAEDNLTFSQAHIKA
SEEQLQQAKRRFEKGFGTITEINEAQADLDMALAEGLEIVNSVEFSRRELEHLIGTYPDELCKLAPEKLVLARPTPGRVE
SWIDRAHGESPRSSAAHLEMQIAKKEIEKQKASRYPTIDLVAGRSYSESENNYSIGSTYETYSISLQMSMPIYTGGYASA
SIRQAKAKWLKAGEQFFWQERSIESEVRKYYNTVISTIAQIQAYEQAVKSREIALDGTKKGFGAGLRSNVDVLDALQNLL
AARRNLAKSRYQYILARLSLKQTAGALSPADIEEINGWFATAKTAK
>Mature_446_residues
MKSFWYVILIAGSFVSPPLYAAPVTISEAYLKARDHDALLGAAKADNLVYKEEVGKARAGLRPSVRLNASRGRNGTQHGY
MGLYEAPDFYNTVVYGVTLRQPLINLSNIAEYQQAKAVAAKSDAELRKEEADLIVRLAETYCNALYAEDNLTFSQAHIKA
SEEQLQQAKRRFEKGFGTITEINEAQADLDMALAEGLEIVNSVEFSRRELEHLIGTYPDELCKLAPEKLVLARPTPGRVE
SWIDRAHGESPRSSAAHLEMQIAKKEIEKQKASRYPTIDLVAGRSYSESENNYSIGSTYETYSISLQMSMPIYTGGYASA
SIRQAKAKWLKAGEQFFWQERSIESEVRKYYNTVISTIAQIQAYEQAVKSREIALDGTKKGFGAGLRSNVDVLDALQNLL
AARRNLAKSRYQYILARLSLKQTAGALSPADIEEINGWFATAKTAK

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=427, Percent_Identity=28.5714285714286, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49638; Mature: 49638

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFWYVILIAGSFVSPPLYAAPVTISEAYLKARDHDALLGAAKADNLVYKEEVGKARAG
CCCEEEEEEEECCCCCCCCEECCCHHHHHHHHHCCCHHHHCCHHHCCCHHHHHHHHHHCC
LRPSVRLNASRGRNGTQHGYMGLYEAPDFYNTVVYGVTLRQPLINLSNIAEYQQAKAVAA
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
KSDAELRKEEADLIVRLAETYCNALYAEDNLTFSQAHIKASEEQLQQAKRRFEKGFGTIT
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEHHHHHHCHHHHHHHHHHHHHHCCCCHH
EINEAQADLDMALAEGLEIVNSVEFSRRELEHLIGTYPDELCKLAPEKLVLARPTPGRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCHHHH
SWIDRAHGESPRSSAAHLEMQIAKKEIEKQKASRYPTIDLVAGRSYSESENNYSIGSTYE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEE
TYSISLQMSMPIYTGGYASASIRQAKAKWLKAGEQFFWQERSIESEVRKYYNTVISTIAQ
EEEEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAYEQAVKSREIALDGTKKGFGAGLRSNVDVLDALQNLLAARRNLAKSRYQYILARLSL
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQTAGALSPADIEEINGWFATAKTAK
HHHCCCCCCCCHHHCCCCEEECCCCC
>Mature Secondary Structure
MKSFWYVILIAGSFVSPPLYAAPVTISEAYLKARDHDALLGAAKADNLVYKEEVGKARAG
CCCEEEEEEEECCCCCCCCEECCCHHHHHHHHHCCCHHHHCCHHHCCCHHHHHHHHHHCC
LRPSVRLNASRGRNGTQHGYMGLYEAPDFYNTVVYGVTLRQPLINLSNIAEYQQAKAVAA
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
KSDAELRKEEADLIVRLAETYCNALYAEDNLTFSQAHIKASEEQLQQAKRRFEKGFGTIT
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEHHHHHHCHHHHHHHHHHHHHHCCCCHH
EINEAQADLDMALAEGLEIVNSVEFSRRELEHLIGTYPDELCKLAPEKLVLARPTPGRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCHHHH
SWIDRAHGESPRSSAAHLEMQIAKKEIEKQKASRYPTIDLVAGRSYSESENNYSIGSTYE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEE
TYSISLQMSMPIYTGGYASASIRQAKAKWLKAGEQFFWQERSIESEVRKYYNTVISTIAQ
EEEEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAYEQAVKSREIALDGTKKGFGAGLRSNVDVLDALQNLLAARRNLAKSRYQYILARLSL
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQTAGALSPADIEEINGWFATAKTAK
HHHCCCCCCCCHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]