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The map label for this gene is trmD [H]

Identifier: 119357324

GI number: 119357324

Start: 1730861

End: 1731562

Strand: Reverse

Name: trmD [H]

Synonym: Cpha266_1522

Alternate gene names: 119357324

Gene position: 1731562-1730861 (Counterclockwise)

Preceding gene: 119357325

Following gene: 119357323

Centisome position: 55.25

GC content: 49.43

Gene sequence:

>702_bases
ATGGATCCGATGCGGATTGATGTTATTTCCGTTATCCCCGGTTTTTTCGATTCGGCCCTTCATAACGGTCTTTTGAGCAT
AGCCCGGAAGAAAGAGTATGTGGAAATTCATGTCCATAACCTTCACGACTATGGACTGGGACGGTACCGACAGGTTGACG
ATACGCCGTTTGGAGGTGGAGCAGGCATGGTTCTGCGTCCGGAACCGGTGTTTTCATGTATTGAAGCCCTCACGGGCGAG
AGGGAGTATGATGCGGTGATTTTTCCAACACCGGACGGCAAGCCGTTTGAACAGGCTCTTGCAAACCGGTTTTCCCGCAT
GAAAAACCTGATTATTCTTTGTGGGCATTACAAGGCCCTTGACGAAAGGGTTCGTCAGACGCTTGTAACCATGGAGTTGT
CAGTTGGTGATGTAGTGGTTTCGGGCGGAGAGATACCGGCACTGCTTATTATGGATGCCGTTGTGCGATTGATTCCGGGA
GTGCTTGGCGACAGCGAGTCGGCCTTGACGGATTCTTTTCAGACAGGAATGCTTGATAGCGTCTATTACACCCGGCCTTC
AGAGTTCAGAGGAATGAAAGTCCCTGATGTTTTGTTGTCAGGCAATCATCGTAATATTGAGCAGTGGCGTTATGATAATG
CCTTGCAGCGAACAACCCGGAGACGTCCCGATCTGCTGGATATGAAAAGTTTTGAAGATTGA

Upstream 100 bases:

>100_bases
AAGAGATTGATATGGCCGGCAGATATATGGTTGTGCCCCGTTTTGACGAGTTTTTGTGAAAGATTGTTCTTGACAAGATT
GTTTCAGGGAATCTCAGTGT

Downstream 100 bases:

>100_bases
GTAAAAAAATAACGTAAATATTTGTCGTCATGGATCAGTTAATTCAGTTAGTTGAAGCTTCAGAAGCCAGAAATGATTTT
CCGGCTATTAATCCGGGTGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDPMRIDVISVIPGFFDSALHNGLLSIARKKEYVEIHVHNLHDYGLGRYRQVDDTPFGGGAGMVLRPEPVFSCIEALTGE
REYDAVIFPTPDGKPFEQALANRFSRMKNLIILCGHYKALDERVRQTLVTMELSVGDVVVSGGEIPALLIMDAVVRLIPG
VLGDSESALTDSFQTGMLDSVYYTRPSEFRGMKVPDVLLSGNHRNIEQWRYDNALQRTTRRRPDLLDMKSFED

Sequences:

>Translated_233_residues
MDPMRIDVISVIPGFFDSALHNGLLSIARKKEYVEIHVHNLHDYGLGRYRQVDDTPFGGGAGMVLRPEPVFSCIEALTGE
REYDAVIFPTPDGKPFEQALANRFSRMKNLIILCGHYKALDERVRQTLVTMELSVGDVVVSGGEIPALLIMDAVVRLIPG
VLGDSESALTDSFQTGMLDSVYYTRPSEFRGMKVPDVLLSGNHRNIEQWRYDNALQRTTRRRPDLLDMKSFED
>Mature_233_residues
MDPMRIDVISVIPGFFDSALHNGLLSIARKKEYVEIHVHNLHDYGLGRYRQVDDTPFGGGAGMVLRPEPVFSCIEALTGE
REYDAVIFPTPDGKPFEQALANRFSRMKNLIILCGHYKALDERVRQTLVTMELSVGDVVVSGGEIPALLIMDAVVRLIPG
VLGDSESALTDSFQTGMLDSVYYTRPSEFRGMKVPDVLLSGNHRNIEQWRYDNALQRTTRRRPDLLDMKSFED

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=42.6666666666667, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26217; Mature: 26217

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPMRIDVISVIPGFFDSALHNGLLSIARKKEYVEIHVHNLHDYGLGRYRQVDDTPFGGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
AGMVLRPEPVFSCIEALTGEREYDAVIFPTPDGKPFEQALANRFSRMKNLIILCGHYKAL
CCEEECCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCHHHH
DERVRQTLVTMELSVGDVVVSGGEIPALLIMDAVVRLIPGVLGDSESALTDSFQTGMLDS
HHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHH
VYYTRPSEFRGMKVPDVLLSGNHRNIEQWRYDNALQRTTRRRPDLLDMKSFED
HEECCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDPMRIDVISVIPGFFDSALHNGLLSIARKKEYVEIHVHNLHDYGLGRYRQVDDTPFGGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
AGMVLRPEPVFSCIEALTGEREYDAVIFPTPDGKPFEQALANRFSRMKNLIILCGHYKAL
CCEEECCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCHHHH
DERVRQTLVTMELSVGDVVVSGGEIPALLIMDAVVRLIPGVLGDSESALTDSFQTGMLDS
HHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHH
VYYTRPSEFRGMKVPDVLLSGNHRNIEQWRYDNALQRTTRRRPDLLDMKSFED
HEECCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA