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The map label for this gene is lipA

Identifier: 119357090

GI number: 119357090

Start: 1455568

End: 1456431

Strand: Reverse

Name: lipA

Synonym: Cpha266_1277

Alternate gene names: 119357090

Gene position: 1456431-1455568 (Counterclockwise)

Preceding gene: 119357093

Following gene: 119357089

Centisome position: 46.47

GC content: 49.54

Gene sequence:

>864_bases
ATGAGCAACGTACTCGCAAGAAAACCTGACTGGCTGAAACTGAGACTTTCTGCTCATGGCGAGTTTGCTGCAACGCGGCA
ACTTCTTGAGCAACGGAATCTCAACACGGTTTGTCGTTCGGCTATGTGTCCGAATCTTCAGGAGTGCTGGTCAAGGGGTA
CGGCAACTTTTCTGCTTCTTGGTAACATCTGTACCCGAACCTGCCGGTTTTGTGCGGTTGGCACAGCATCCATTCCTCCG
ATGCCTGATTCTCTTGAACCGGAGAACATAGCCGAAGCAGTAGAAATAATGAATCTGAACCATGTGGTACTGACAAGCGT
CAACCGTGACGATCTTGCTGATGGCGGTGCCCGACACTGGCAGAAAACCATGCAGGCTGTACGCCAGCGTAACCCCAAGG
TGACGCTTGAGTGTCTTATACCTGATTTTCAGGCACAAACCGAGGCGCTTGATATCGTGCTTGCTGAGGCCCCTGAAGTT
TTGAATCATAATATCGAAACGGTTCCCTCGATTTACCATATTGTTCGTCCAGAGGCAAACTACTCATCATCTCTGAACAT
AATTCGCCGGGCAAAAGAACATTTTAACCTTACAACCAAGTCAGGGTTGATGGTCGGGATGGGTGAAACTTTCGATGAGG
TGGTTCAGTCGCTGCACGATCTTGTACAGCATGGTTGCGACATGGTAACCATCGGCCAGTATTTGCAGCCAAGCGCAAGT
CACATCCCGGTCAACCGCTATGTGACACCTGAGGAATTTGACCGGTATAAAACTGTTGCAGAGTCACTTGGGCTCCGAAA
TGTGCGTTCAGGCCCCTTTGTCAGAAGTTCATACCTCGCAGAAACCCTTTCACCGGATCATTAA

Upstream 100 bases:

>100_bases
TTTTAGATGGCGCTCTTGCATATTCGCCTTCATATTTCCCTATATTACGGCTTCTTGAAAATGATAAATGAATTCCCGCA
TCAAAGCATCTCAGCCATTT

Downstream 100 bases:

>100_bases
ACTCAAAACATTACGATTTATGCAGATCACCCCTCCAATGATGGTTTTTGCCTACTGGGCAATTGCAGTTCTCATCGGAT
TCGTTTTTTTTCGCAAGGAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSNVLARKPDWLKLRLSAHGEFAATRQLLEQRNLNTVCRSAMCPNLQECWSRGTATFLLLGNICTRTCRFCAVGTASIPP
MPDSLEPENIAEAVEIMNLNHVVLTSVNRDDLADGGARHWQKTMQAVRQRNPKVTLECLIPDFQAQTEALDIVLAEAPEV
LNHNIETVPSIYHIVRPEANYSSSLNIIRRAKEHFNLTTKSGLMVGMGETFDEVVQSLHDLVQHGCDMVTIGQYLQPSAS
HIPVNRYVTPEEFDRYKTVAESLGLRNVRSGPFVRSSYLAETLSPDH

Sequences:

>Translated_287_residues
MSNVLARKPDWLKLRLSAHGEFAATRQLLEQRNLNTVCRSAMCPNLQECWSRGTATFLLLGNICTRTCRFCAVGTASIPP
MPDSLEPENIAEAVEIMNLNHVVLTSVNRDDLADGGARHWQKTMQAVRQRNPKVTLECLIPDFQAQTEALDIVLAEAPEV
LNHNIETVPSIYHIVRPEANYSSSLNIIRRAKEHFNLTTKSGLMVGMGETFDEVVQSLHDLVQHGCDMVTIGQYLQPSAS
HIPVNRYVTPEEFDRYKTVAESLGLRNVRSGPFVRSSYLAETLSPDH
>Mature_286_residues
SNVLARKPDWLKLRLSAHGEFAATRQLLEQRNLNTVCRSAMCPNLQECWSRGTATFLLLGNICTRTCRFCAVGTASIPPM
PDSLEPENIAEAVEIMNLNHVVLTSVNRDDLADGGARHWQKTMQAVRQRNPKVTLECLIPDFQAQTEALDIVLAEAPEVL
NHNIETVPSIYHIVRPEANYSSSLNIIRRAKEHFNLTTKSGLMVGMGETFDEVVQSLHDLVQHGCDMVTIGQYLQPSASH
IPVNRYVTPEEFDRYKTVAESLGLRNVRSGPFVRSSYLAETLSPDH

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=281, Percent_Identity=42.7046263345196, Blast_Score=247, Evalue=8e-66,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=42.2764227642276, Blast_Score=214, Evalue=9e-56,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=48.5714285714286, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=46.8253968253968, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=279, Percent_Identity=43.010752688172, Blast_Score=241, Evalue=7e-65,
Organism=Drosophila melanogaster, GI221513272, Length=281, Percent_Identity=43.0604982206406, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CHLPD (A1BFY3)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911734.1
- STRING:   A1BFY3
- GeneID:   4570487
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1277
- NMPDR:   fig|290317.7.peg.1349
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32109; Mature: 31978

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVLARKPDWLKLRLSAHGEFAATRQLLEQRNLNTVCRSAMCPNLQECWSRGTATFLLL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHCCCCEEEHHH
GNICTRTCRFCAVGTASIPPMPDSLEPENIAEAVEIMNLNHVVLTSVNRDDLADGGARHW
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCCHHHHH
QKTMQAVRQRNPKVTLECLIPDFQAQTEALDIVLAEAPEVLNHNIETVPSIYHIVRPEAN
HHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCCC
YSSSLNIIRRAKEHFNLTTKSGLMVGMGETFDEVVQSLHDLVQHGCDMVTIGQYLQPSAS
CHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCC
HIPVNRYVTPEEFDRYKTVAESLGLRNVRSGPFVRSSYLAETLSPDH
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNVLARKPDWLKLRLSAHGEFAATRQLLEQRNLNTVCRSAMCPNLQECWSRGTATFLLL
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHCCCCEEEHHH
GNICTRTCRFCAVGTASIPPMPDSLEPENIAEAVEIMNLNHVVLTSVNRDDLADGGARHW
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCCHHHHH
QKTMQAVRQRNPKVTLECLIPDFQAQTEALDIVLAEAPEVLNHNIETVPSIYHIVRPEAN
HHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCCC
YSSSLNIIRRAKEHFNLTTKSGLMVGMGETFDEVVQSLHDLVQHGCDMVTIGQYLQPSAS
CHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCC
HIPVNRYVTPEEFDRYKTVAESLGLRNVRSGPFVRSSYLAETLSPDH
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA