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The map label for this gene is ytsJ [H]

Identifier: 119357084

GI number: 119357084

Start: 1449477

End: 1450637

Strand: Reverse

Name: ytsJ [H]

Synonym: Cpha266_1271

Alternate gene names: 119357084

Gene position: 1450637-1449477 (Counterclockwise)

Preceding gene: 119357087

Following gene: 119357080

Centisome position: 46.29

GC content: 46.51

Gene sequence:

>1161_bases
ATGGATTATTCAAAAAAATCTCTTGAATTGCATCGTAAAACACGAGGCAAAATAGAAATCAAGTCAAAAGTAAGCCTGAA
AACCAAAAACGACCTTTCGCTTGCCTATACTCCCGGAGTAGCCGCTGTCTGTATGGAAATAGGGCGTGACCGGCAGCTAT
CCTATACCCTGACCAATCGGGCAAGTCAGGTAGCGATTGTTTCCGACGGCACAGCTATTCTTGGCCTTGGTGATCTGGGT
CCGGAAGCGGCAATGCCGGTAATGGAGGGAAAGGCGATCATCTTTAAGGAGTTTGCCGATATTGATGCTATTCCGCTCTG
CATAAATTCTACCGAAGTTGAAGATATCGTAAAATTCTGCAAACTTATTGAGCCAAGTTTTGCGGGTATCAACCTCGAAG
ATATAGCTGCGCCAAGATGTTTTGAGATTCTCGAAAGGCTTGAACGTGAACTCTCAATACCGGTTTTTCATGACGATCAG
GACGGAACGGCCATCATAACTCTGGCAGCCATCATCAATGCCTGCCGACTGTTCGATCGTAAGCTGAACGACCTGCATGT
TGTCATCAATGGTGCCGGAGCGGCAGGTATTGCGATTGCAAAACTTCTGATTCATGCCGGACTTAAAGAGGTTGTTCTGG
TAGATACCAAAGGTGCGATCTACTCCGGTCGACCGGAAATGAACAGAATAAAACAGGGAATTGCTGAAAAAACGAACCGG
GCGAAATATCAAGGGGATCTGAAAAGTTCGTTGATCGGAGCAGATGTTTTTATCGGCGTCTCAAGGGGAAATATTCTGAC
TGAAGAGATGATTAAGGGGATGAACGACGCCCCTTTTATTTTTGCCATGGCTAATCCGGAACCGGAGATCATGCCCGATC
GTGCTTATAAGGCCGGGGCTGCCGTTGTCGGTACCGGACGGTCGGATTTGCCCAACCAGGTTAATAATGCGTTGGTTTTC
CCCGGGTTGTTTAAAGGATTGCTCAAATCCGGGATCACTGAAGTTACTCCTGAAATCAAAATGGCGGTAGCGTTTTCTAT
TGCCCACTCTATTGAACCAACACGTGACAGCCTCATGCCTACCGCTTTTAATAAACAAGTCGTAACAGGCATTGTGGATG
TTCTGTCTGGCATTCAGGCAAAAAAAGCAACTGCTGAGTAA

Upstream 100 bases:

>100_bases
TGATACACTTTTCCTGGAAAAACTGGTGAGGATTAAACGGCAAATCATGATCGATCCCGATGTATCGGGACTCATGACAA
TAAACAAGAAGTACTCATAA

Downstream 100 bases:

>100_bases
AAAATAACCGGCCACTATTGTTTTTTCTTCGCGTATTGACTCAGGCAGGGTTAAAAACCGTCTTCCAACAAGTTTATTGG
TTGTGGCTCCCCTGCCGTAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MDYSKKSLELHRKTRGKIEIKSKVSLKTKNDLSLAYTPGVAAVCMEIGRDRQLSYTLTNRASQVAIVSDGTAILGLGDLG
PEAAMPVMEGKAIIFKEFADIDAIPLCINSTEVEDIVKFCKLIEPSFAGINLEDIAAPRCFEILERLERELSIPVFHDDQ
DGTAIITLAAIINACRLFDRKLNDLHVVINGAGAAGIAIAKLLIHAGLKEVVLVDTKGAIYSGRPEMNRIKQGIAEKTNR
AKYQGDLKSSLIGADVFIGVSRGNILTEEMIKGMNDAPFIFAMANPEPEIMPDRAYKAGAAVVGTGRSDLPNQVNNALVF
PGLFKGLLKSGITEVTPEIKMAVAFSIAHSIEPTRDSLMPTAFNKQVVTGIVDVLSGIQAKKATAE

Sequences:

>Translated_386_residues
MDYSKKSLELHRKTRGKIEIKSKVSLKTKNDLSLAYTPGVAAVCMEIGRDRQLSYTLTNRASQVAIVSDGTAILGLGDLG
PEAAMPVMEGKAIIFKEFADIDAIPLCINSTEVEDIVKFCKLIEPSFAGINLEDIAAPRCFEILERLERELSIPVFHDDQ
DGTAIITLAAIINACRLFDRKLNDLHVVINGAGAAGIAIAKLLIHAGLKEVVLVDTKGAIYSGRPEMNRIKQGIAEKTNR
AKYQGDLKSSLIGADVFIGVSRGNILTEEMIKGMNDAPFIFAMANPEPEIMPDRAYKAGAAVVGTGRSDLPNQVNNALVF
PGLFKGLLKSGITEVTPEIKMAVAFSIAHSIEPTRDSLMPTAFNKQVVTGIVDVLSGIQAKKATAE
>Mature_386_residues
MDYSKKSLELHRKTRGKIEIKSKVSLKTKNDLSLAYTPGVAAVCMEIGRDRQLSYTLTNRASQVAIVSDGTAILGLGDLG
PEAAMPVMEGKAIIFKEFADIDAIPLCINSTEVEDIVKFCKLIEPSFAGINLEDIAAPRCFEILERLERELSIPVFHDDQ
DGTAIITLAAIINACRLFDRKLNDLHVVINGAGAAGIAIAKLLIHAGLKEVVLVDTKGAIYSGRPEMNRIKQGIAEKTNR
AKYQGDLKSSLIGADVFIGVSRGNILTEEMIKGMNDAPFIFAMANPEPEIMPDRAYKAGAAVVGTGRSDLPNQVNNALVF
PGLFKGLLKSGITEVTPEIKMAVAFSIAHSIEPTRDSLMPTAFNKQVVTGIVDVLSGIQAKKATAE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=399, Percent_Identity=26.3157894736842, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI62420882, Length=396, Percent_Identity=26.2626262626263, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI62420880, Length=396, Percent_Identity=26.2626262626263, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI239049447, Length=396, Percent_Identity=26.2626262626263, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI4505145, Length=426, Percent_Identity=22.7699530516432, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788806, Length=367, Percent_Identity=43.5967302452316, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87081919, Length=400, Percent_Identity=25.25, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537199, Length=389, Percent_Identity=25.706940874036, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=274, Percent_Identity=27.007299270073, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356279, Length=409, Percent_Identity=25.1833740831296, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281362672, Length=409, Percent_Identity=25.1833740831296, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281362674, Length=409, Percent_Identity=25.1833740831296, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI78707242, Length=392, Percent_Identity=25.765306122449, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707238, Length=392, Percent_Identity=25.765306122449, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707240, Length=392, Percent_Identity=25.765306122449, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI78707232, Length=338, Percent_Identity=27.810650887574, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363503, Length=338, Percent_Identity=27.810650887574, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363505, Length=338, Percent_Identity=27.810650887574, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI78707236, Length=338, Percent_Identity=27.810650887574, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24646386, Length=398, Percent_Identity=24.8743718592965, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24646388, Length=398, Percent_Identity=24.8743718592965, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 41564; Mature: 41564

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYSKKSLELHRKTRGKIEIKSKVSLKTKNDLSLAYTPGVAAVCMEIGRDRQLSYTLTNR
CCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEECCCHHHHHHHHCCCCEEEEEECCC
ASQVAIVSDGTAILGLGDLGPEAAMPVMEGKAIIFKEFADIDAIPLCINSTEVEDIVKFC
CCEEEEEECCCEEEEECCCCCHHCCCHHCCCEEEEEHHCCCCCEEEEECCCCHHHHHHHH
KLIEPSFAGINLEDIAAPRCFEILERLERELSIPVFHDDQDGTAIITLAAIINACRLFDR
HHHCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
KLNDLHVVINGAGAAGIAIAKLLIHAGLKEVVLVDTKGAIYSGRPEMNRIKQGIAEKTNR
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHH
AKYQGDLKSSLIGADVFIGVSRGNILTEEMIKGMNDAPFIFAMANPEPEIMPDRAYKAGA
HHHCCCHHHHHCCCEEEEEECCCCEEHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCC
AVVGTGRSDLPNQVNNALVFPGLFKGLLKSGITEVTPEIKMAVAFSIAHSIEPTRDSLMP
EEEECCCCCCCHHHCCEEECHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCHHHCCC
TAFNKQVVTGIVDVLSGIQAKKATAE
CCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDYSKKSLELHRKTRGKIEIKSKVSLKTKNDLSLAYTPGVAAVCMEIGRDRQLSYTLTNR
CCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEECCCHHHHHHHHCCCCEEEEEECCC
ASQVAIVSDGTAILGLGDLGPEAAMPVMEGKAIIFKEFADIDAIPLCINSTEVEDIVKFC
CCEEEEEECCCEEEEECCCCCHHCCCHHCCCEEEEEHHCCCCCEEEEECCCCHHHHHHHH
KLIEPSFAGINLEDIAAPRCFEILERLERELSIPVFHDDQDGTAIITLAAIINACRLFDR
HHHCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
KLNDLHVVINGAGAAGIAIAKLLIHAGLKEVVLVDTKGAIYSGRPEMNRIKQGIAEKTNR
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHH
AKYQGDLKSSLIGADVFIGVSRGNILTEEMIKGMNDAPFIFAMANPEPEIMPDRAYKAGA
HHHCCCHHHHHCCCEEEEEECCCCEEHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCC
AVVGTGRSDLPNQVNNALVFPGLFKGLLKSGITEVTPEIKMAVAFSIAHSIEPTRDSLMP
EEEECCCCCCCHHHCCEEECHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCHHHCCC
TAFNKQVVTGIVDVLSGIQAKKATAE
CCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]