Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 119356994

GI number: 119356994

Start: 1328224

End: 1329825

Strand: Reverse

Name: prfC [H]

Synonym: Cpha266_1177

Alternate gene names: 119356994

Gene position: 1329825-1328224 (Counterclockwise)

Preceding gene: 119356999

Following gene: 119356993

Centisome position: 42.43

GC content: 47.88

Gene sequence:

>1602_bases
ATGAATGCAGAATTAAAAAGAGAAATTTCAAAACGACGAACATTTGCCATCATCAGCCATCCTGATGCCGGTAAAACAAC
GCTGACAGAAAAATTCCTGCTTTTCGGCGGCGCCATCCAGACCGCCGGTGCGGTAAAAAGCAATAAAATCCGTAAAACCG
CCACCAGCGACTTTATGGAAATTGAAAAGCAGCGCGGCATATCCGTTGCGACATCGGTCATGGGGTTTGAGTATGCAGGC
AAGAGAATCAATATTCTCGACACTCCCGGTCACAAGGATTTTGCTGAAGACACCTATCGTACCCTTACGGCTGTTGACAG
CGTTATTCTTGTGGTCGACTGCGTAAAAGGGGTCGAGGAACAAACGGAAAAGCTGATGAGCGTATGCCGCATGCGCCACA
CTCCTGTAATCATTTTTATCAATAAAATGGATCGGGAGGGCAAAGCTCCCATCGAACTGCTTGACGAACTTGAGGAAAAA
CTGCAGATCAATACCCGACCGCTGAGCTGGCCGATCAGTCAGGGACAAACGTTCAAAGGGGTTTACAATCTCTACACTTC
AGCATTAAACCTTTTTGAAGCAAACTCTTCAAGAATTTCCGAAACCCTTGTCAAGATCCAGAGCCCCCAGGATCCGGAAC
TTGAAAAATGGATTCCGGAGAGCTTCTGCAAAAAACTCCGTGAAGATATAGCCCTGATTGAAGGAGTTTATGAACCGTTC
AACGAAGAACTCTACCGCGACGGCCTGCTCGCTCCGGTTTTTTTCGGCAGCGCCATCAATAATTTCGGAGTAAAAGAGCT
GCTTGAAACCTTTCTTTCCATTGCGCCCTGCCCCTATGAACGCGATGCGAGAGAGCGAACCGTCTTTGCGTCGGAAGAGG
CCATGAGCGGATTTGTCTTTAAAATCCACGCAAACCTCGACCCCAATCACCGCGACAGAACGGCTTTTTTCAAAATATGT
TCCGGAAGATTCGAACGCAACAAGTTCTACTATCACACCAGGCTGAAAAAAAAGATAAGGTTTTCCAGCCCGACTCATTT
TATGGCAAATGAGAAAAGCATTATTGATGACGCATGGCCCGGGGATGTGATCGGCCTGTACGATAACGGCTCGCTGAAAA
TCGGAGACACGCTTACTGAAGGCGAAGAGCTCCAGTTCCGGGGAATCCCGAGCTTTTCTCCGGAGATTTTCAAGCTGCTT
GAAAACCACGATCCCATGAAAACAAAACAGCTCGACAAGGGAATCCGCCAGCTCACTGAAGAAGGCGTCGCCCAGCTCTT
TGTACAGCATGGAAACCGCAAAATTGTCGGCACCGTAGGAGAGCTGCAGTTCGATGTCATAAAATTCCGACTTGAACATG
AATATGGCGCACTTTGCGCTTTCACTCCCCTTCGCTACAAGAAAGCACTCTGGGTCACCAGCGATAACAAGGAGAGTCTT
GGAGAGTTTTTGCGCCGAAAATCCAACCTGATCGTTTTTGACCGGGAGGATCATCCGGTTTTTCTTGCCGAAAGCGAATG
GATGCTGAAAACAGCACAGGAAGACTACCCCGATATCGAATTTCACACCACATCGGAATTTAAAACCGACCTTGCCATGT
AA

Upstream 100 bases:

>100_bases
ATTCTGTTTTTAACCCCTATCTTTGTATCTTTGCGCCTTCGATTAAGTTTTCACTTTCCACAAGAGGAAAAGCACCGTAA
CAGAAAACATGCCGTATTAA

Downstream 100 bases:

>100_bases
GGGACGATGCTCTGCAAGGATGCAAAAACAGTACATCCCGTTTGAGATAACGAAAAAAGGCGACTACGGGAACGTAATCG
CCTTCTCTGGTGTCGGGGTG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MNAELKREISKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKSNKIRKTATSDFMEIEKQRGISVATSVMGFEYAG
KRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQTEKLMSVCRMRHTPVIIFINKMDREGKAPIELLDELEEK
LQINTRPLSWPISQGQTFKGVYNLYTSALNLFEANSSRISETLVKIQSPQDPELEKWIPESFCKKLREDIALIEGVYEPF
NEELYRDGLLAPVFFGSAINNFGVKELLETFLSIAPCPYERDARERTVFASEEAMSGFVFKIHANLDPNHRDRTAFFKIC
SGRFERNKFYYHTRLKKKIRFSSPTHFMANEKSIIDDAWPGDVIGLYDNGSLKIGDTLTEGEELQFRGIPSFSPEIFKLL
ENHDPMKTKQLDKGIRQLTEEGVAQLFVQHGNRKIVGTVGELQFDVIKFRLEHEYGALCAFTPLRYKKALWVTSDNKESL
GEFLRRKSNLIVFDREDHPVFLAESEWMLKTAQEDYPDIEFHTTSEFKTDLAM

Sequences:

>Translated_533_residues
MNAELKREISKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKSNKIRKTATSDFMEIEKQRGISVATSVMGFEYAG
KRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQTEKLMSVCRMRHTPVIIFINKMDREGKAPIELLDELEEK
LQINTRPLSWPISQGQTFKGVYNLYTSALNLFEANSSRISETLVKIQSPQDPELEKWIPESFCKKLREDIALIEGVYEPF
NEELYRDGLLAPVFFGSAINNFGVKELLETFLSIAPCPYERDARERTVFASEEAMSGFVFKIHANLDPNHRDRTAFFKIC
SGRFERNKFYYHTRLKKKIRFSSPTHFMANEKSIIDDAWPGDVIGLYDNGSLKIGDTLTEGEELQFRGIPSFSPEIFKLL
ENHDPMKTKQLDKGIRQLTEEGVAQLFVQHGNRKIVGTVGELQFDVIKFRLEHEYGALCAFTPLRYKKALWVTSDNKESL
GEFLRRKSNLIVFDREDHPVFLAESEWMLKTAQEDYPDIEFHTTSEFKTDLAM
>Mature_533_residues
MNAELKREISKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKSNKIRKTATSDFMEIEKQRGISVATSVMGFEYAG
KRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQTEKLMSVCRMRHTPVIIFINKMDREGKAPIELLDELEEK
LQINTRPLSWPISQGQTFKGVYNLYTSALNLFEANSSRISETLVKIQSPQDPELEKWIPESFCKKLREDIALIEGVYEPF
NEELYRDGLLAPVFFGSAINNFGVKELLETFLSIAPCPYERDARERTVFASEEAMSGFVFKIHANLDPNHRDRTAFFKIC
SGRFERNKFYYHTRLKKKIRFSSPTHFMANEKSIIDDAWPGDVIGLYDNGSLKIGDTLTEGEELQFRGIPSFSPEIFKLL
ENHDPMKTKQLDKGIRQLTEEGVAQLFVQHGNRKIVGTVGELQFDVIKFRLEHEYGALCAFTPLRYKKALWVTSDNKESL
GEFLRRKSNLIVFDREDHPVFLAESEWMLKTAQEDYPDIEFHTTSEFKTDLAM

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=27.2540983606557, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI19923640, Length=546, Percent_Identity=26.5567765567766, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI25306283, Length=335, Percent_Identity=31.044776119403, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306287, Length=295, Percent_Identity=32.5423728813559, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=33.5483870967742, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI94966754, Length=264, Percent_Identity=26.8939393939394, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.2013422818792, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI310132016, Length=122, Percent_Identity=32.7868852459016, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310110807, Length=122, Percent_Identity=32.7868852459016, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310123363, Length=122, Percent_Identity=32.7868852459016, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=46.8330134357006, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1789738, Length=492, Percent_Identity=27.0325203252033, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994988, Length=179, Percent_Identity=34.6368715083799, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=29.746835443038, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=485, Percent_Identity=26.8041237113402, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=182, Percent_Identity=35.7142857142857, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=31.3868613138686, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=184, Percent_Identity=32.6086956521739, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=31.3432835820896, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=31.3432835820896, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=191, Percent_Identity=28.7958115183246, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323098, Length=502, Percent_Identity=24.7011952191235, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322359, Length=544, Percent_Identity=25.1838235294118, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=28.0254777070064, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=28.0254777070064, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=29.7101449275362, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24582462, Length=492, Percent_Identity=28.2520325203252, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI221458488, Length=511, Percent_Identity=26.027397260274, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI78706572, Length=241, Percent_Identity=29.045643153527, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24585709, Length=181, Percent_Identity=30.939226519337, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24585711, Length=181, Percent_Identity=30.939226519337, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24585713, Length=181, Percent_Identity=30.939226519337, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60999; Mature: 60999

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAELKREISKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKSNKIRKTATSDFME
CCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHH
IEKQRGISVATSVMGFEYAGKRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEE
HHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTEKLMSVCRMRHTPVIIFINKMDREGKAPIELLDELEEKLQINTRPLSWPISQGQTFKG
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHH
VYNLYTSALNLFEANSSRISETLVKIQSPQDPELEKWIPESFCKKLREDIALIEGVYEPF
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHH
NEELYRDGLLAPVFFGSAINNFGVKELLETFLSIAPCPYERDARERTVFASEEAMSGFVF
HHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHEEEECHHHCCCEEE
KIHANLDPNHRDRTAFFKICSGRFERNKFYYHTRLKKKIRFSSPTHFMANEKSIIDDAWP
EEECCCCCCCCHHHHHHHHHCCCHHCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCC
GDVIGLYDNGSLKIGDTLTEGEELQFRGIPSFSPEIFKLLENHDPMKTKQLDKGIRQLTE
CCEEEEEECCCEEECCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
EGVAQLFVQHGNRKIVGTVGELQFDVIKFRLEHEYGALCAFTPLRYKKALWVTSDNKESL
HHHHHHHHHCCCEEEEEEHHHHHEEHHHEEECCCCCCEEEECCHHHCEEEEEECCCHHHH
GEFLRRKSNLIVFDREDHPVFLAESEWMLKTAQEDYPDIEFHTTSEFKTDLAM
HHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCEEEECCHHHHHCCC
>Mature Secondary Structure
MNAELKREISKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKSNKIRKTATSDFME
CCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHH
IEKQRGISVATSVMGFEYAGKRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEE
HHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTEKLMSVCRMRHTPVIIFINKMDREGKAPIELLDELEEKLQINTRPLSWPISQGQTFKG
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHH
VYNLYTSALNLFEANSSRISETLVKIQSPQDPELEKWIPESFCKKLREDIALIEGVYEPF
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHH
NEELYRDGLLAPVFFGSAINNFGVKELLETFLSIAPCPYERDARERTVFASEEAMSGFVF
HHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHEEEECHHHCCCEEE
KIHANLDPNHRDRTAFFKICSGRFERNKFYYHTRLKKKIRFSSPTHFMANEKSIIDDAWP
EEECCCCCCCCHHHHHHHHHCCCHHCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCC
GDVIGLYDNGSLKIGDTLTEGEELQFRGIPSFSPEIFKLLENHDPMKTKQLDKGIRQLTE
CCEEEEEECCCEEECCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
EGVAQLFVQHGNRKIVGTVGELQFDVIKFRLEHEYGALCAFTPLRYKKALWVTSDNKESL
HHHHHHHHHCCCEEEEEEHHHHHEEHHHEEECCCCCCEEEECCHHHCEEEEEECCCHHHH
GEFLRRKSNLIVFDREDHPVFLAESEWMLKTAQEDYPDIEFHTTSEFKTDLAM
HHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA