Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 119356986

GI number: 119356986

Start: 1319232

End: 1320041

Strand: Reverse

Name: ksgA

Synonym: Cpha266_1169

Alternate gene names: 119356986

Gene position: 1320041-1319232 (Counterclockwise)

Preceding gene: 119356991

Following gene: 119356985

Centisome position: 42.12

GC content: 45.31

Gene sequence:

>810_bases
ATGATAAAAGTTGAATATAAGCATACAGAAATAGCGGTAAAAAAAAAACTGGGTCAAAACTTTCTCACAGACCGAAATAT
CACTCGTAAAATAGTTACGGAATCCGAAACGAACCCTGACGATACTATTCTGGAGATAGGACCGGGATTCGGGGCATTGA
CGCGGGAAATCAGTACCATTACTCCGCGCTTCACTGTTGTTGAAAAAGACCCGAAGCTTGCCTCATTCATCCGTAACGAG
TACCCTGAACTTACGGTTATAGAGGGAGATTTTCTCACTGTCGACCTTAAAAAAATCGCAGGCGAAAAACCGCTCAGGGT
TCTTGGCAATATACCCTATGCCATTACCAGCCCGATTCTCTTTAAACTGCTTGAAAACCGTCATATTTTATTATCGGCAA
CCCTGATGATGCAACATGAAGTTGCCTTGAGAATAACCGCAAAGCCCCGCACAAAAGATTATGGCATACTTGCCGTACAG
ATGCAGGCGTTCTGTGAAACCAAATATCTCTTTCGTGTCGGACGAAAGGTTTTTCGGCCTCAGCCTGGAGTTGACAGCGC
AGTCATCAGCATGAAACCTAAAGTCAACGATCCGGTCTCTGACAGAGAAGGATTCAGCCGGTTCGTACGCTGCGCATTCC
ATCAGAGAAGAAAAACCCTGCAAAACAATCTGAAAAAAACCTATGAACTTGACCGGGTGGAGAGTTCGGTGCTGAAACAG
CGGGCCGAAGAGCTTTCAATTGATGAATTTTTCCGGCTCTTCGAACAAATCAGACCCCTTATGGTCTCATCGGCTGATCC
GGAAAGCTGA

Upstream 100 bases:

>100_bases
GCCGCCGCTTCGATGTTATTTTCTCCCATTACTTCCTGAATAACTTGAATTGTAAAAAGTATTTCCGTCCGGGTAAGGTG
CTAAAAAAAACGCCGTCATC

Downstream 100 bases:

>100_bases
TTGATCGGAACGCCCCTCTGTTTAAACAATTGTTTTGATTGTTCATGAATGTATTTTTTCTTAACATTGCTTTTTGCATA
AAAGGCTCCTCCTTGAAATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MIKVEYKHTEIAVKKKLGQNFLTDRNITRKIVTESETNPDDTILEIGPGFGALTREISTITPRFTVVEKDPKLASFIRNE
YPELTVIEGDFLTVDLKKIAGEKPLRVLGNIPYAITSPILFKLLENRHILLSATLMMQHEVALRITAKPRTKDYGILAVQ
MQAFCETKYLFRVGRKVFRPQPGVDSAVISMKPKVNDPVSDREGFSRFVRCAFHQRRKTLQNNLKKTYELDRVESSVLKQ
RAEELSIDEFFRLFEQIRPLMVSSADPES

Sequences:

>Translated_269_residues
MIKVEYKHTEIAVKKKLGQNFLTDRNITRKIVTESETNPDDTILEIGPGFGALTREISTITPRFTVVEKDPKLASFIRNE
YPELTVIEGDFLTVDLKKIAGEKPLRVLGNIPYAITSPILFKLLENRHILLSATLMMQHEVALRITAKPRTKDYGILAVQ
MQAFCETKYLFRVGRKVFRPQPGVDSAVISMKPKVNDPVSDREGFSRFVRCAFHQRRKTLQNNLKKTYELDRVESSVLKQ
RAEELSIDEFFRLFEQIRPLMVSSADPES
>Mature_269_residues
MIKVEYKHTEIAVKKKLGQNFLTDRNITRKIVTESETNPDDTILEIGPGFGALTREISTITPRFTVVEKDPKLASFIRNE
YPELTVIEGDFLTVDLKKIAGEKPLRVLGNIPYAITSPILFKLLENRHILLSATLMMQHEVALRITAKPRTKDYGILAVQ
MQAFCETKYLFRVGRKVFRPQPGVDSAVISMKPKVNDPVSDREGFSRFVRCAFHQRRKTLQNNLKKTYELDRVESSVLKQ
RAEELSIDEFFRLFEQIRPLMVSSADPES

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=264, Percent_Identity=31.0606060606061, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI156415992, Length=254, Percent_Identity=29.9212598425197, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1786236, Length=255, Percent_Identity=32.9411764705882, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146882, Length=211, Percent_Identity=33.175355450237, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=260, Percent_Identity=29.2307692307692, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=226, Percent_Identity=29.646017699115, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358017, Length=280, Percent_Identity=30, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21357273, Length=278, Percent_Identity=31.294964028777, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CHLPD (A1BFM9)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911630.1
- ProteinModelPortal:   A1BFM9
- SMR:   A1BFM9
- STRING:   A1BFM9
- GeneID:   4570746
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1169
- NMPDR:   fig|290317.7.peg.1241
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30858; Mature: 30858

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 20-20 BINDING 22-22 BINDING 47-47 BINDING 68-68 BINDING 90-90 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVEYKHTEIAVKKKLGQNFLTDRNITRKIVTESETNPDDTILEIGPGFGALTREISTI
CEEEEECHHHHHHHHHHCHHHHCCCCHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHC
TPRFTVVEKDPKLASFIRNEYPELTVIEGDFLTVDLKKIAGEKPLRVLGNIPYAITSPIL
CCCEEEEECCCHHHHHHHHCCCCEEEEECCEEEEEHHHHCCCCCHHHHCCCCHHHHHHHH
FKLLENRHILLSATLMMQHEVALRITAKPRTKDYGILAVQMQAFCETKYLFRVGRKVFRP
HHHHCCCEEEEEHHHHHHEEEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
QPGVDSAVISMKPKVNDPVSDREGFSRFVRCAFHQRRKTLQNNLKKTYELDRVESSVLKQ
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEELSIDEFFRLFEQIRPLMVSSADPES
HHHHCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIKVEYKHTEIAVKKKLGQNFLTDRNITRKIVTESETNPDDTILEIGPGFGALTREISTI
CEEEEECHHHHHHHHHHCHHHHCCCCHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHC
TPRFTVVEKDPKLASFIRNEYPELTVIEGDFLTVDLKKIAGEKPLRVLGNIPYAITSPIL
CCCEEEEECCCHHHHHHHHCCCCEEEEECCEEEEEHHHHCCCCCHHHHCCCCHHHHHHHH
FKLLENRHILLSATLMMQHEVALRITAKPRTKDYGILAVQMQAFCETKYLFRVGRKVFRP
HHHHCCCEEEEEHHHHHHEEEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
QPGVDSAVISMKPKVNDPVSDREGFSRFVRCAFHQRRKTLQNNLKKTYELDRVESSVLKQ
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEELSIDEFFRLFEQIRPLMVSSADPES
HHHHCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA