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The map label for this gene is purB [H]

Identifier: 119356929

GI number: 119356929

Start: 1251617

End: 1252921

Strand: Reverse

Name: purB [H]

Synonym: Cpha266_1108

Alternate gene names: 119356929

Gene position: 1252921-1251617 (Counterclockwise)

Preceding gene: 119356930

Following gene: 119356928

Centisome position: 39.98

GC content: 49.89

Gene sequence:

>1305_bases
TTGATACCACGTTATTCGCCAAAAGACATTGCTGCAATCTGGACAGAAGAGGCAAAATTCCAGAGATGGCTCCAGCTTGA
AATCGCCGCCGTCGAAGCAAGAATGGAAGCCGGAGTAGTGCCCGAAGAGGCGCTGGGAACAATAAAGGAAAAAGCGAAGT
TTGACGTCGCTGAAATTCTTGAAATCGAAAAAGAGACAAAACATGACGTCATCGCATTTCTGACCAATGTCGCCGGTTAC
GTCGGCCCTGACTCCCGGTATATCCATGAAGGTCTTACCTCTTCGGACGTTGTCGATACCTGTCTTGCCATGCAGATGCG
CGATGCCGGTCATATTCTTCTCGGGGATATTGAGCAGTTGATTACTGTTCTCGGCAAAAGAGCCGTTGAGTTCAAATATA
CCCTCGAAATGGGACGTACGCACGGCATTCATGCCGAACCAACAACTTTCGGCCTCAAGCTGCTGCTCTGGCACCAGGAG
ATGCTCAGACATCTGGATCGGATGAAAAGAGCTGTTGCCGTGATCTCCGTCGGAAAAATCTCGGGGGCTGTAGGTACCTA
TCAGCACCTTTCTCCTGAAATCGAAGCCGTTGTCTGTGAAAAACTCGGCCTTCAGCCCTCTTCGATATCGACACAGATCC
TGCAACGCGACCGTCATGCGGAATTTGCCACTACCCTTGCGCTCATTGCCTCCTCGATTGAAAAGTTCTCCGTTGAACTT
CGGCACCTGCAGCGCACCGAAGTCCGCGAAGCTGAAGAGTATTTCAGCAAAGGACAAAAAGGCAGCTCGGCCATGCCGCA
CAAACGCAATCCAATCACCTTCGAAAGACTGACCGGTCTTGCCCGTGTAGTCCGCTCAAATTCACTTGCTGCCATGGAAG
ATGTTGCGCTCTGGCATGAACGCGATATTTCACACTCATCCGTCGAGCGAATCATCATGCCTGATTCCACCATCGCGCTC
TGCTACATGCTCAGAACGTTCAGCGAATCCCTTGAAACCCTTCTGGTCTATCCTGATCGCATGGAAGAGAACTTCAACTC
ATCTTTCGGTCTGACAACATCACAAACCCTTCTCCTTGCGTTAACGGGAAAAGGGCTGACACGTGAAAAAGCCTATGGGC
TCGTTCAGAGAAACGCCATGGAAAGCTGGTCGAAAAGGATTCACCTTCGTGAGCTCGTGCTGGAGGATAAAGAGATTCTT
GAACATGTAACGCCTCAGGAAATCACCGAACTCTTCAGTTTCGACACCATTCTCGAAAAACTCAAAACCAGCGTTGATAC
TATTTTTCAGCGTAACGGGTTCTGA

Upstream 100 bases:

>100_bases
CGCAATCGGCATGACCCGATTCATCTGTGGTTTTTCAGGTTCACGTACACTATATTCCCTGCATTACCCGTTACCTTGCA
ACGATTAAAATCATGACACC

Downstream 100 bases:

>100_bases
AACTCCCTTTAATTCCCAAACGCACTGTCAAAAACAGATCGTTTGGGAATTAACAACCGATCGTAACAGTTCATTAATAA
GATCAGCATCTTATTACAAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIPRYSPKDIAAIWTEEAKFQRWLQLEIAAVEARMEAGVVPEEALGTIKEKAKFDVAEILEIEKETKHDVIAFLTNVAGY
VGPDSRYIHEGLTSSDVVDTCLAMQMRDAGHILLGDIEQLITVLGKRAVEFKYTLEMGRTHGIHAEPTTFGLKLLLWHQE
MLRHLDRMKRAVAVISVGKISGAVGTYQHLSPEIEAVVCEKLGLQPSSISTQILQRDRHAEFATTLALIASSIEKFSVEL
RHLQRTEVREAEEYFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSLAAMEDVALWHERDISHSSVERIIMPDSTIAL
CYMLRTFSESLETLLVYPDRMEENFNSSFGLTTSQTLLLALTGKGLTREKAYGLVQRNAMESWSKRIHLRELVLEDKEIL
EHVTPQEITELFSFDTILEKLKTSVDTIFQRNGF

Sequences:

>Translated_434_residues
MIPRYSPKDIAAIWTEEAKFQRWLQLEIAAVEARMEAGVVPEEALGTIKEKAKFDVAEILEIEKETKHDVIAFLTNVAGY
VGPDSRYIHEGLTSSDVVDTCLAMQMRDAGHILLGDIEQLITVLGKRAVEFKYTLEMGRTHGIHAEPTTFGLKLLLWHQE
MLRHLDRMKRAVAVISVGKISGAVGTYQHLSPEIEAVVCEKLGLQPSSISTQILQRDRHAEFATTLALIASSIEKFSVEL
RHLQRTEVREAEEYFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSLAAMEDVALWHERDISHSSVERIIMPDSTIAL
CYMLRTFSESLETLLVYPDRMEENFNSSFGLTTSQTLLLALTGKGLTREKAYGLVQRNAMESWSKRIHLRELVLEDKEIL
EHVTPQEITELFSFDTILEKLKTSVDTIFQRNGF
>Mature_434_residues
MIPRYSPKDIAAIWTEEAKFQRWLQLEIAAVEARMEAGVVPEEALGTIKEKAKFDVAEILEIEKETKHDVIAFLTNVAGY
VGPDSRYIHEGLTSSDVVDTCLAMQMRDAGHILLGDIEQLITVLGKRAVEFKYTLEMGRTHGIHAEPTTFGLKLLLWHQE
MLRHLDRMKRAVAVISVGKISGAVGTYQHLSPEIEAVVCEKLGLQPSSISTQILQRDRHAEFATTLALIASSIEKFSVEL
RHLQRTEVREAEEYFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSLAAMEDVALWHERDISHSSVERIIMPDSTIAL
CYMLRTFSESLETLLVYPDRMEENFNSSFGLTTSQTLLLALTGKGLTREKAYGLVQRNAMESWSKRIHLRELVLEDKEIL
EHVTPQEITELFSFDTILEKLKTSVDTIFQRNGF

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=384, Percent_Identity=27.8645833333333, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.0839895013123, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1787376, Length=344, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=397, Percent_Identity=27.7078085642317, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=361, Percent_Identity=28.808864265928, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=386, Percent_Identity=27.979274611399, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=26.3157894736842, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49097; Mature: 49097

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSPKDIAAIWTEEAKFQRWLQLEIAAVEARMEAGVVPEEALGTIKEKAKFDVAEIL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHH
EIEKETKHDVIAFLTNVAGYVGPDSRYIHEGLTSSDVVDTCLAMQMRDAGHILLGDIEQL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
ITVLGKRAVEFKYTLEMGRTHGIHAEPTTFGLKLLLWHQEMLRHLDRMKRAVAVISVGKI
HHHHCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYQHLSPEIEAVVCEKLGLQPSSISTQILQRDRHAEFATTLALIASSIEKFSVEL
CCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVREAEEYFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSLAAMEDVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RDISHSSVERIIMPDSTIALCYMLRTFSESLETLLVYPDRMEENFNSSFGLTTSQTLLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCCCCCCCCCEEEEE
LTGKGLTREKAYGLVQRNAMESWSKRIHLRELVLEDKEILEHVTPQEITELFSFDTILEK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LKTSVDTIFQRNGF
HHHHHHHHHHCCCC
>Mature Secondary Structure
MIPRYSPKDIAAIWTEEAKFQRWLQLEIAAVEARMEAGVVPEEALGTIKEKAKFDVAEIL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHH
EIEKETKHDVIAFLTNVAGYVGPDSRYIHEGLTSSDVVDTCLAMQMRDAGHILLGDIEQL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
ITVLGKRAVEFKYTLEMGRTHGIHAEPTTFGLKLLLWHQEMLRHLDRMKRAVAVISVGKI
HHHHCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYQHLSPEIEAVVCEKLGLQPSSISTQILQRDRHAEFATTLALIASSIEKFSVEL
CCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVREAEEYFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSLAAMEDVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RDISHSSVERIIMPDSTIALCYMLRTFSESLETLLVYPDRMEENFNSSFGLTTSQTLLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCCCCCCCCCEEEEE
LTGKGLTREKAYGLVQRNAMESWSKRIHLRELVLEDKEILEHVTPQEITELFSFDTILEK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LKTSVDTIFQRNGF
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]