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The map label for this gene is miaA

Identifier: 119356902

GI number: 119356902

Start: 1222197

End: 1223156

Strand: Reverse

Name: miaA

Synonym: Cpha266_1081

Alternate gene names: 119356902

Gene position: 1223156-1222197 (Counterclockwise)

Preceding gene: 119356903

Following gene: 119356901

Centisome position: 39.03

GC content: 45.52

Gene sequence:

>960_bases
ATGCATGATTCCGACCGGCAAAAAACGATTCTCGCCATTCTGGGGCCTACCGCATCCGGAAAATCGGCACTCGCCTTCAG
CATAGCAAAAGAGATAAACGCAGAGATCATCTCCGCCGACTCAAGGCAAATTTATCGTGAACTGAATATCGGTACCGCAA
AACCATCTCAAGAAACACTGCGGCAGATAAAACACCACTTTGTTAATGAATTATCCATAGGCGACCCCTTTACAGCCGGA
AATTTTGCCAGAGAGGCATCAGCAAGAATCCGGAACATTCTCCATTCAGGAAAAAATGTCCTTATTGCCGGAGGCTCAAC
CCTTTATCTTGAAGCTTTGCTCAATGGATTTGCCGATCTGCCGCCTGCTGATACAGAAAAAAGACAACAACTTGCCATTG
AACTTGAAACCTTCGGAAGCAAGCACCTGTTTGAACGTCTCAGGCAACTTGACCCTCTGCAGGCGGCAACGCTTGATCAC
ACCAAAACCCAACGACTGGTAAGAAGTCTTGAAATAATTGAACTGAGCGGGCATACCGTCACAGAGATGCAGAAAAAGCA
TATTCAGCCTCTTGCCGACATCAACGTCATCACCTGCGGACTCTCATTGCCGCGACCACTGCTTTATGAAAAAATAAACA
GGAGAACCGACCAGATGCTTGCCTCGGGACTTCTGCAGGAAGCGGTAGCCCTCTTTACCAAATACTACCCATATTGCGAT
GTAATAACAACAAATGCGCTGCAAACGGTAGGGTATCAGGAACTCTTTGCATACCTTGATGAAAAAACTGATTTCAAGAC
CGCAATTACCCTGATTAAACAGCATACGCGTAATTATGCAAAACGCCAGTTGACTTTTTTTAAAAATAGGCTTAACGTTA
ACTGGATGGATGCTCCCGAAAATGAACATGAGCTCTCCATGTTAACCACCTCATGTTGCAGGATGATTACCGGAAACTGA

Upstream 100 bases:

>100_bases
CGAATCGAAGGCTGCTACTATAACGTAGCCGGATTGCCGCTTTCAAGGGTTCATTCCGTGCTGCACTCACACTTTTTCGC
CGAAAAATAAAGGAAAGCCC

Downstream 100 bases:

>100_bases
TTCGTTCTGTCAGCGGGGTGTTGCAAATCATCTGCACAAGCATTGATCAAAGGCTCTGAAATGTGTTTCTTCCTGTAAAT
TGATCAATACGTTCTTCATC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MHDSDRQKTILAILGPTASGKSALAFSIAKEINAEIISADSRQIYRELNIGTAKPSQETLRQIKHHFVNELSIGDPFTAG
NFAREASARIRNILHSGKNVLIAGGSTLYLEALLNGFADLPPADTEKRQQLAIELETFGSKHLFERLRQLDPLQAATLDH
TKTQRLVRSLEIIELSGHTVTEMQKKHIQPLADINVITCGLSLPRPLLYEKINRRTDQMLASGLLQEAVALFTKYYPYCD
VITTNALQTVGYQELFAYLDEKTDFKTAITLIKQHTRNYAKRQLTFFKNRLNVNWMDAPENEHELSMLTTSCCRMITGN

Sequences:

>Translated_319_residues
MHDSDRQKTILAILGPTASGKSALAFSIAKEINAEIISADSRQIYRELNIGTAKPSQETLRQIKHHFVNELSIGDPFTAG
NFAREASARIRNILHSGKNVLIAGGSTLYLEALLNGFADLPPADTEKRQQLAIELETFGSKHLFERLRQLDPLQAATLDH
TKTQRLVRSLEIIELSGHTVTEMQKKHIQPLADINVITCGLSLPRPLLYEKINRRTDQMLASGLLQEAVALFTKYYPYCD
VITTNALQTVGYQELFAYLDEKTDFKTAITLIKQHTRNYAKRQLTFFKNRLNVNWMDAPENEHELSMLTTSCCRMITGN
>Mature_319_residues
MHDSDRQKTILAILGPTASGKSALAFSIAKEINAEIISADSRQIYRELNIGTAKPSQETLRQIKHHFVNELSIGDPFTAG
NFAREASARIRNILHSGKNVLIAGGSTLYLEALLNGFADLPPADTEKRQQLAIELETFGSKHLFERLRQLDPLQAATLDH
TKTQRLVRSLEIIELSGHTVTEMQKKHIQPLADINVITCGLSLPRPLLYEKINRRTDQMLASGLLQEAVALFTKYYPYCD
VITTNALQTVGYQELFAYLDEKTDFKTAITLIKQHTRNYAKRQLTFFKNRLNVNWMDAPENEHELSMLTTSCCRMITGN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=30.6709265175719, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=34.9673202614379, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=25.9868421052632, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=29.8305084745763, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24649830, Length=335, Percent_Identity=25.6716417910448, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLPD (A1BFE5)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911546.1
- ProteinModelPortal:   A1BFE5
- SMR:   A1BFE5
- STRING:   A1BFE5
- GeneID:   4570025
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1081
- NMPDR:   fig|290317.7.peg.1147
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK637545
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35958; Mature: 35958

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDSDRQKTILAILGPTASGKSALAFSIAKEINAEIISADSRQIYRELNIGTAKPSQETL
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHCCCCCCCCHHHH
RQIKHHFVNELSIGDPFTAGNFAREASARIRNILHSGKNVLIAGGSTLYLEALLNGFADL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCC
PPADTEKRQQLAIELETFGSKHLFERLRQLDPLQAATLDHTKTQRLVRSLEIIELSGHTV
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHEEECCCHH
TEMQKKHIQPLADINVITCGLSLPRPLLYEKINRRTDQMLASGLLQEAVALFTKYYPYCD
HHHHHHHCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VITTNALQTVGYQELFAYLDEKTDFKTAITLIKQHTRNYAKRQLTFFKNRLNVNWMDAPE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
NEHELSMLTTSCCRMITGN
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHDSDRQKTILAILGPTASGKSALAFSIAKEINAEIISADSRQIYRELNIGTAKPSQETL
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHCCCCCCCCHHHH
RQIKHHFVNELSIGDPFTAGNFAREASARIRNILHSGKNVLIAGGSTLYLEALLNGFADL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCC
PPADTEKRQQLAIELETFGSKHLFERLRQLDPLQAATLDHTKTQRLVRSLEIIELSGHTV
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHEEECCCHH
TEMQKKHIQPLADINVITCGLSLPRPLLYEKINRRTDQMLASGLLQEAVALFTKYYPYCD
HHHHHHHCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VITTNALQTVGYQELFAYLDEKTDFKTAITLIKQHTRNYAKRQLTFFKNRLNVNWMDAPE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
NEHELSMLTTSCCRMITGN
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA