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The map label for this gene is ygcM [C]

Identifier: 119356805

GI number: 119356805

Start: 1119100

End: 1119558

Strand: Reverse

Name: ygcM [C]

Synonym: Cpha266_0977

Alternate gene names: 119356805

Gene position: 1119558-1119100 (Counterclockwise)

Preceding gene: 119356808

Following gene: 119356804

Centisome position: 35.72

GC content: 39.65

Gene sequence:

>459_bases
ATGCTCCACATGAACGCTCCCCTTCAAAGAAAAAGAAAAATTTATGTAACCAGAACTATCGGCTTCAATGCTGCGCATCG
TCTTTTCAATCCTGATTTTTCAGATCAGGAAAACCACAAAATTTATGGGAAATGCAGCAATGAACATGGTCACGGACATA
ATTATGAACTTGAAATCACTCTGTATGGAACCATTGATCCTCAGACAGGCTTTCTTTTTGACTTGAAACAACTCAAAAGC
ATTCTGGAAGAAGAGATCACTGAACGATTTGACCACAAACACCTTAACTTCGACGTCAGAGAGATGCAGGATTGCGTGCC
GACTACCGAACTGCTTGCTGTCGTCATATGGGATATTCTTGACCTTCGATTACAACAATTCAACAACAGAGAGATAACTC
TCTATGAAGTAAAAATCTATGAAACAGGAAAAAATGCCGTCAGATATCTCGGTCAGTAA

Upstream 100 bases:

>100_bases
TTGATACAAAAATAACTATCTTTTGTCACTTTCCGCAAACAGAAGTCTTCGATACTGCACAGGATTTTTTGTTCCCGACG
AAACCAAGCGAAAACTTTTT

Downstream 100 bases:

>100_bases
AACTCCGTCATCCTTAAATTCCGGAGGATTTTGTGAAGATGATGAGTGCCTCATGGATGGTGATACGGCCAGTCCGGAAG
AGATAAGCAATCTTTCAGGA

Product: putative 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MLHMNAPLQRKRKIYVTRTIGFNAAHRLFNPDFSDQENHKIYGKCSNEHGHGHNYELEITLYGTIDPQTGFLFDLKQLKS
ILEEEITERFDHKHLNFDVREMQDCVPTTELLAVVIWDILDLRLQQFNNREITLYEVKIYETGKNAVRYLGQ

Sequences:

>Translated_152_residues
MLHMNAPLQRKRKIYVTRTIGFNAAHRLFNPDFSDQENHKIYGKCSNEHGHGHNYELEITLYGTIDPQTGFLFDLKQLKS
ILEEEITERFDHKHLNFDVREMQDCVPTTELLAVVIWDILDLRLQQFNNREITLYEVKIYETGKNAVRYLGQ
>Mature_152_residues
MLHMNAPLQRKRKIYVTRTIGFNAAHRLFNPDFSDQENHKIYGKCSNEHGHGHNYELEITLYGTIDPQTGFLFDLKQLKS
ILEEEITERFDHKHLNFDVREMQDCVPTTELLAVVIWDILDLRLQQFNNREITLYEVKIYETGKNAVRYLGQ

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=143, Percent_Identity=46.1538461538462, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115532224, Length=138, Percent_Identity=36.231884057971, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI115532226, Length=138, Percent_Identity=36.231884057971, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19550074, Length=137, Percent_Identity=47.4452554744526, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24585378, Length=137, Percent_Identity=47.4452554744526, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 17996; Mature: 17996

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHMNAPLQRKRKIYVTRTIGFNAAHRLFNPDFSDQENHKIYGKCSNEHGHGHNYELEIT
CCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEE
LYGTIDPQTGFLFDLKQLKSILEEEITERFDHKHLNFDVREMQDCVPTTELLAVVIWDIL
EEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
DLRLQQFNNREITLYEVKIYETGKNAVRYLGQ
HHHHHHCCCCEEEEEEEEEEECCCHHHHHCCC
>Mature Secondary Structure
MLHMNAPLQRKRKIYVTRTIGFNAAHRLFNPDFSDQENHKIYGKCSNEHGHGHNYELEIT
CCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEE
LYGTIDPQTGFLFDLKQLKSILEEEITERFDHKHLNFDVREMQDCVPTTELLAVVIWDIL
EEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
DLRLQQFNNREITLYEVKIYETGKNAVRYLGQ
HHHHHHCCCCEEEEEEEEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA