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The map label for this gene is rpoE [H]

Identifier: 119356699

GI number: 119356699

Start: 991130

End: 991759

Strand: Reverse

Name: rpoE [H]

Synonym: Cpha266_0868

Alternate gene names: 119356699

Gene position: 991759-991130 (Counterclockwise)

Preceding gene: 119356700

Following gene: 119356698

Centisome position: 31.65

GC content: 41.43

Gene sequence:

>630_bases
ATGAATACCCTGGAATCATCAGATAAAAAAACCGGAAAAGTTAAAACAGCGCCTCTTTCCCGTGAAGAGCAGCAGAAACA
GCTTGAGTTTCAGCAGGAAGCGATTGCTCATATCAACTCCCTGTATAACTATGCCCTGCATCTGACCATGAATCAGGATG
ATGCTGAAGATCTTGTTCAGGAAACCTATCTGAAAGCATATAAATTCTTCAATTCTTTTGAACGGGGAACCAACTGCAAG
GCCTGGCTGTTCAAAATTCTCAAGAATAATTATATTAATCGTTTTCGAAAAAATTCAAGGGAACCGGGCAAGGTTGATTA
CGATCTGATAAAAGATTTTTATCATTCGATAAAAGATAGCCGTCGTGATACAACGGAAACTGACTCGGACTATTTTCACT
CGCTCCTCCACGAAGAAGTCTATCAGGCGCTTCAATCCCTTCCCGAGGAGTTCAGAGAGGTTATCCAGCTGTGCGATATT
GAAGGCTTTACCTATGAGGAGATAGCTAATATGGTTGAAAGTCCGATTGGAACTGTTCGATCAAGACTGTATAGAGGACG
TAAGCTGCTTCGCGGACAACTTGAAGAATATGCGAAAAAATACGGATTTAACACCGATAATGCCGATTAG

Upstream 100 bases:

>100_bases
TATTCAGGTTAAAGTTGGAAAGCTGTGTTGATTTTAATTAATTATTGAAATGTATGCATTCGAACCTGTCCCGGCGACAC
AGGAAACCAGACCACTTTAT

Downstream 100 bases:

>100_bases
AAGTTTTTTCACCCTGAACACTGTTTCCTATGAATTGTAAAAAAGCCATGGTACTCATGAGTTGTGCCATCGATGGTGAA
CTCACTCCTGCTGAAGAGCT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNTLESSDKKTGKVKTAPLSREEQQKQLEFQQEAIAHINSLYNYALHLTMNQDDAEDLVQETYLKAYKFFNSFERGTNCK
AWLFKILKNNYINRFRKNSREPGKVDYDLIKDFYHSIKDSRRDTTETDSDYFHSLLHEEVYQALQSLPEEFREVIQLCDI
EGFTYEEIANMVESPIGTVRSRLYRGRKLLRGQLEEYAKKYGFNTDNAD

Sequences:

>Translated_209_residues
MNTLESSDKKTGKVKTAPLSREEQQKQLEFQQEAIAHINSLYNYALHLTMNQDDAEDLVQETYLKAYKFFNSFERGTNCK
AWLFKILKNNYINRFRKNSREPGKVDYDLIKDFYHSIKDSRRDTTETDSDYFHSLLHEEVYQALQSLPEEFREVIQLCDI
EGFTYEEIANMVESPIGTVRSRLYRGRKLLRGQLEEYAKKYGFNTDNAD
>Mature_209_residues
MNTLESSDKKTGKVKTAPLSREEQQKQLEFQQEAIAHINSLYNYALHLTMNQDDAEDLVQETYLKAYKFFNSFERGTNCK
AWLFKILKNNYINRFRKNSREPGKVDYDLIKDFYHSIKDSRRDTTETDSDYFHSLLHEEVYQALQSLPEEFREVIQLCDI
EGFTYEEIANMVESPIGTVRSRLYRGRKLLRGQLEEYAKKYGFNTDNAD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=147, Percent_Identity=29.9319727891156, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24716; Mature: 24716

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLESSDKKTGKVKTAPLSREEQQKQLEFQQEAIAHINSLYNYALHLTMNQDDAEDLVQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHH
ETYLKAYKFFNSFERGTNCKAWLFKILKNNYINRFRKNSREPGKVDYDLIKDFYHSIKDS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
RRDTTETDSDYFHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANMVESPIGTVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRLYRGRKLLRGQLEEYAKKYGFNTDNAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNTLESSDKKTGKVKTAPLSREEQQKQLEFQQEAIAHINSLYNYALHLTMNQDDAEDLVQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHH
ETYLKAYKFFNSFERGTNCKAWLFKILKNNYINRFRKNSREPGKVDYDLIKDFYHSIKDS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
RRDTTETDSDYFHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANMVESPIGTVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRLYRGRKLLRGQLEEYAKKYGFNTDNAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]