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The map label for this gene is leuB [H]

Identifier: 119356681

GI number: 119356681

Start: 973107

End: 974165

Strand: Reverse

Name: leuB [H]

Synonym: Cpha266_0850

Alternate gene names: 119356681

Gene position: 974165-973107 (Counterclockwise)

Preceding gene: 119356682

Following gene: 119356680

Centisome position: 31.08

GC content: 52.03

Gene sequence:

>1059_bases
ATGTATAAGATTGTTTCAATACCGGGTGACGGTATCGGCCCGGAAGTCGTTGCTGGCGCCGTTACCGTGCTCAGAAAAAT
CTCTGAAAAGCACGGTTTCGAAATCCGCATTGAGGAACACCCTTTTGGAGGCGCATCCTACGACCTGCACGGAACCATGC
TTACCGATCAAACGCTTGAAGCGTGCAAAAACTGCGATGCCGTTCTGCTTGGAGCCGTAGGAGGACCGAAATGGGAGAAT
CTCCCGCATGAGCACAAACCCGAAGCAGCTTTGCTCAAACTCAGAAAGTCGCTCGGTCTCTTCGCTAACCTGAGGCCGGC
AAAAGTCTATGATCCCCTTGTTGACGCTTCGTCTCTCAAGGCAGAAGTCGTGCGGGGAACAGATTTTCTTGTCTTCAGGG
AGCTGATCGGCGGCATCTATTTCGGAGAGCCGAGAGGATATGACGAAAACAGAGGGTGGAACACCATGGTCTATGAACGC
CATGAAGTTGAGCGCATAGCCCGCCTTGCCTTTGAAGCTGCCCAGAAGCGTGGCGGACGGGTTATCTCCATAGACAAAGC
CAATGTGCTTGAAGTTTCCCAGTTCTGGAGAAATGTCGTACATGAGGTACACCGGGAGTTTCCCGACATAGAACTCAGCG
ACATGTATGTTGACAACGCTGCCATGCAGATTGTCAGAAACCCCTTGCAATTTGACGTTATCGTCACAGGAAACCTTTTT
GGTGACATACTCAGCGACATTGCGGGCATGATCACCGGTAGTCTTGGAATGCTTCCTTCGGCCAGCATCGGAACAAGCCA
TGCTCTCTACGAACCTATTCACGGCAGTGCGCCGGACATTGCAGGAAAAAACATTGCGAACCCCATTGCGACCATCGCAT
CGGTAGCCATGATGTTCGAACACAGCTTCTGCATGCCTGATATAGCCGAAGAGATCAGCCAGGCTATTGTATCGGCCCTT
GCGGCCGGCCTCAGAACCGCAGATATTGCCGGGGCGGGTGACAGAATTGTTTCAACTACTGAAATGACCGAAGCCATCGT
CACCAGCCTCGGTTCATAA

Upstream 100 bases:

>100_bases
CAGGGAGGAGAACCGTACCCATTCAATCGAAAAAGTCGGCGCAAGACTACGCGAGATGATGAGCTGGCTTATCAAAAAAT
AACCGCATCCTATAAAACGT

Downstream 100 bases:

>100_bases
ACAAAGAAGAACGGATGAAAACGTGAGAGAAAAAATCCTTGTATTTGACACAACACTTCGCGACGGTGAACAGTCACCCG
GAGCATCCCTGAATGTCCAG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MYKIVSIPGDGIGPEVVAGAVTVLRKISEKHGFEIRIEEHPFGGASYDLHGTMLTDQTLEACKNCDAVLLGAVGGPKWEN
LPHEHKPEAALLKLRKSLGLFANLRPAKVYDPLVDASSLKAEVVRGTDFLVFRELIGGIYFGEPRGYDENRGWNTMVYER
HEVERIARLAFEAAQKRGGRVISIDKANVLEVSQFWRNVVHEVHREFPDIELSDMYVDNAAMQIVRNPLQFDVIVTGNLF
GDILSDIAGMITGSLGMLPSASIGTSHALYEPIHGSAPDIAGKNIANPIATIASVAMMFEHSFCMPDIAEEISQAIVSAL
AAGLRTADIAGAGDRIVSTTEMTEAIVTSLGS

Sequences:

>Translated_352_residues
MYKIVSIPGDGIGPEVVAGAVTVLRKISEKHGFEIRIEEHPFGGASYDLHGTMLTDQTLEACKNCDAVLLGAVGGPKWEN
LPHEHKPEAALLKLRKSLGLFANLRPAKVYDPLVDASSLKAEVVRGTDFLVFRELIGGIYFGEPRGYDENRGWNTMVYER
HEVERIARLAFEAAQKRGGRVISIDKANVLEVSQFWRNVVHEVHREFPDIELSDMYVDNAAMQIVRNPLQFDVIVTGNLF
GDILSDIAGMITGSLGMLPSASIGTSHALYEPIHGSAPDIAGKNIANPIATIASVAMMFEHSFCMPDIAEEISQAIVSAL
AAGLRTADIAGAGDRIVSTTEMTEAIVTSLGS
>Mature_352_residues
MYKIVSIPGDGIGPEVVAGAVTVLRKISEKHGFEIRIEEHPFGGASYDLHGTMLTDQTLEACKNCDAVLLGAVGGPKWEN
LPHEHKPEAALLKLRKSLGLFANLRPAKVYDPLVDASSLKAEVVRGTDFLVFRELIGGIYFGEPRGYDENRGWNTMVYER
HEVERIARLAFEAAQKRGGRVISIDKANVLEVSQFWRNVVHEVHREFPDIELSDMYVDNAAMQIVRNPLQFDVIVTGNLF
GDILSDIAGMITGSLGMLPSASIGTSHALYEPIHGSAPDIAGKNIANPIATIASVAMMFEHSFCMPDIAEEISQAIVSAL
AAGLRTADIAGAGDRIVSTTEMTEAIVTSLGS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=332, Percent_Identity=31.6265060240964, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=27.5766016713092, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28178821, Length=310, Percent_Identity=29.3548387096774, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI4758582, Length=353, Percent_Identity=29.4617563739377, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178838, Length=325, Percent_Identity=29.8461538461538, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI28178819, Length=149, Percent_Identity=36.241610738255, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=52.2727272727273, Blast_Score=350, Evalue=6e-98,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=35.7340720221607, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787381, Length=399, Percent_Identity=25.062656641604, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=337, Percent_Identity=30.5637982195846, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=353, Percent_Identity=30.028328611898, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=360, Percent_Identity=28.8888888888889, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=47.2067039106145, Blast_Score=289, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=34.6260387811634, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=33.4269662921348, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=361, Percent_Identity=31.8559556786704, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=29.6610169491525, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=29.6610169491525, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078635, Length=363, Percent_Identity=30.3030303030303, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078633, Length=361, Percent_Identity=29.3628808864266, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650122, Length=361, Percent_Identity=29.3628808864266, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078637, Length=361, Percent_Identity=29.3628808864266, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078639, Length=356, Percent_Identity=29.7752808988764, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=27.8551532033426, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=29.5454545454545, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=29.5454545454545, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI20130355, Length=190, Percent_Identity=26.8421052631579, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38126; Mature: 38126

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKIVSIPGDGIGPEVVAGAVTVLRKISEKHGFEIRIEEHPFGGASYDLHGTMLTDQTLE
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCEEECHHHHH
ACKNCDAVLLGAVGGPKWENLPHEHKPEAALLKLRKSLGLFANLRPAKVYDPLVDASSLK
HHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHH
AEVVRGTDFLVFRELIGGIYFGEPRGYDENRGWNTMVYERHEVERIARLAFEAAQKRGGR
HHHHCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCE
VISIDKANVLEVSQFWRNVVHEVHREFPDIELSDMYVDNAAMQIVRNPLQFDVIVTGNLF
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHH
GDILSDIAGMITGSLGMLPSASIGTSHALYEPIHGSAPDIAGKNIANPIATIASVAMMFE
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
HSFCMPDIAEEISQAIVSALAAGLRTADIAGAGDRIVSTTEMTEAIVTSLGS
HCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKIVSIPGDGIGPEVVAGAVTVLRKISEKHGFEIRIEEHPFGGASYDLHGTMLTDQTLE
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCEEECHHHHH
ACKNCDAVLLGAVGGPKWENLPHEHKPEAALLKLRKSLGLFANLRPAKVYDPLVDASSLK
HHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHH
AEVVRGTDFLVFRELIGGIYFGEPRGYDENRGWNTMVYERHEVERIARLAFEAAQKRGGR
HHHHCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCE
VISIDKANVLEVSQFWRNVVHEVHREFPDIELSDMYVDNAAMQIVRNPLQFDVIVTGNLF
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHH
GDILSDIAGMITGSLGMLPSASIGTSHALYEPIHGSAPDIAGKNIANPIATIASVAMMFE
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
HSFCMPDIAEEISQAIVSALAAGLRTADIAGAGDRIVSTTEMTEAIVTSLGS
HCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA