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The map label for this gene is nadE

Identifier: 119356641

GI number: 119356641

Start: 923388

End: 924221

Strand: Direct

Name: nadE

Synonym: Cpha266_0810

Alternate gene names: 119356641

Gene position: 923388-924221 (Clockwise)

Preceding gene: 119356640

Following gene: 119356651

Centisome position: 29.46

GC content: 45.68

Gene sequence:

>834_bases
ATGAATTCTCTTGATTTGCATCTTGATTACGGTATTGTTGAAGATATTCTCAAAACATTTCTTCTTAACGAGATCAGGAA
ATTCGGATTTCGATCCGTTGTCCTTGGTCTTTCCGGAGGCATTGATTCTGCTGTGGTCTGCGAACTTGCCTCTCGCGCAC
TTGGTTCCGATCAGGTGCTTGCGCTCATGATGCCTTACAGGTCGAGCAGCACTGACAGTATCGTTCATGCACAACTGCTT
GTCGAAAAACTTGGTATCCGGGCTGAAACTTGTTCAATAACTGCTGCCGTTGACGCTTTTTTCGAAGGGGTTCCCGAAGA
GGATCGTCTGAGAAGAGGTAATATTATGGCTCGTACGAGAATGGTTTATCTCTATGATGTTTCGGCACGTCAGAACAGTC
TGGTGGTAGGAACCAGCAATAAAACCGAACTTCTGCTTGGGTATGGAACGCTTTTTGGAGATATGGCTTCAGCCGTCAAT
CCTGTCGGCGATCTCTACAAAACCCAGATACGTGGTCTTGCGCGTCATCTCGGGATTCCGGAACAGTTGATTACTAAAAC
GCCCTCAGCCGATCTCTGGGAGGGCCAGAGCGATGAGGCTGATCTTGGATTTTCTTATGATGAGGTGGATCATCTTCTGT
TTATGATGCTTGAAAAAAGAATGGATAAAGCAGCAATTATCGAGCAGGGCGTTTCGGAGATATTTTATGACAGGGTGAGA
AAAATGGTGGTCAGGAACCAGTACAAGAGAATGATGCCTGTTATTGCAAAAATTTCCTCAAGAACTCCCGGAATAGATTT
TCGCTATGCAAGGGATTGGCAGGAGGTGAAGTAG

Upstream 100 bases:

>100_bases
GTTGTTCAGGAGTACAGCGCTTCTGTTTACTGGAGTCAACATGGCATCGTTTCTTGTTGTTATGGCTCTTGTCAGGTTGT
TCAATTTTTAGTGTTGAAGC

Downstream 100 bases:

>100_bases
TTCTGCTCGGGATCAGTTCAGAACGATCTGAGAACGGTATCGATAAGAAAATGCTTGTTATCACTTTTCGGATAATCCGT
TGTGAAGTGCAGGCCTCTTG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNSLDLHLDYGIVEDILKTFLLNEIRKFGFRSVVLGLSGGIDSAVVCELASRALGSDQVLALMMPYRSSSTDSIVHAQLL
VEKLGIRAETCSITAAVDAFFEGVPEEDRLRRGNIMARTRMVYLYDVSARQNSLVVGTSNKTELLLGYGTLFGDMASAVN
PVGDLYKTQIRGLARHLGIPEQLITKTPSADLWEGQSDEADLGFSYDEVDHLLFMMLEKRMDKAAIIEQGVSEIFYDRVR
KMVVRNQYKRMMPVIAKISSRTPGIDFRYARDWQEVK

Sequences:

>Translated_277_residues
MNSLDLHLDYGIVEDILKTFLLNEIRKFGFRSVVLGLSGGIDSAVVCELASRALGSDQVLALMMPYRSSSTDSIVHAQLL
VEKLGIRAETCSITAAVDAFFEGVPEEDRLRRGNIMARTRMVYLYDVSARQNSLVVGTSNKTELLLGYGTLFGDMASAVN
PVGDLYKTQIRGLARHLGIPEQLITKTPSADLWEGQSDEADLGFSYDEVDHLLFMMLEKRMDKAAIIEQGVSEIFYDRVR
KMVVRNQYKRMMPVIAKISSRTPGIDFRYARDWQEVK
>Mature_277_residues
MNSLDLHLDYGIVEDILKTFLLNEIRKFGFRSVVLGLSGGIDSAVVCELASRALGSDQVLALMMPYRSSSTDSIVHAQLL
VEKLGIRAETCSITAAVDAFFEGVPEEDRLRRGNIMARTRMVYLYDVSARQNSLVVGTSNKTELLLGYGTLFGDMASAVN
PVGDLYKTQIRGLARHLGIPEQLITKTPSADLWEGQSDEADLGFSYDEVDHLLFMMLEKRMDKAAIIEQGVSEIFYDRVR
KMVVRNQYKRMMPVIAKISSRTPGIDFRYARDWQEVK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=221, Percent_Identity=32.579185520362, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_CHLPD (A1BEN4)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911285.1
- ProteinModelPortal:   A1BEN4
- SMR:   A1BEN4
- STRING:   A1BEN4
- GeneID:   4570308
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0810
- NMPDR:   fig|290317.7.peg.859
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   PRK13980
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 31086; Mature: 31086

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLDLHLDYGIVEDILKTFLLNEIRKFGFRSVVLGLSGGIDSAVVCELASRALGSDQVL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
ALMMPYRSSSTDSIVHAQLLVEKLGIRAETCSITAAVDAFFEGVPEEDRLRRGNIMARTR
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEE
MVYLYDVSARQNSLVVGTSNKTELLLGYGTLFGDMASAVNPVGDLYKTQIRGLARHLGIP
EEEEEEECCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
EQLITKTPSADLWEGQSDEADLGFSYDEVDHLLFMMLEKRMDKAAIIEQGVSEIFYDRVR
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMVVRNQYKRMMPVIAKISSRTPGIDFRYARDWQEVK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MNSLDLHLDYGIVEDILKTFLLNEIRKFGFRSVVLGLSGGIDSAVVCELASRALGSDQVL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
ALMMPYRSSSTDSIVHAQLLVEKLGIRAETCSITAAVDAFFEGVPEEDRLRRGNIMARTR
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEE
MVYLYDVSARQNSLVVGTSNKTELLLGYGTLFGDMASAVNPVGDLYKTQIRGLARHLGIP
EEEEEEECCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
EQLITKTPSADLWEGQSDEADLGFSYDEVDHLLFMMLEKRMDKAAIIEQGVSEIFYDRVR
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMVVRNQYKRMMPVIAKISSRTPGIDFRYARDWQEVK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA