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The map label for this gene is proP [H]

Identifier: 119356547

GI number: 119356547

Start: 811985

End: 813562

Strand: Reverse

Name: proP [H]

Synonym: Cpha266_0712

Alternate gene names: 119356547

Gene position: 813562-811985 (Counterclockwise)

Preceding gene: 119356548

Following gene: 119356546

Centisome position: 25.96

GC content: 49.62

Gene sequence:

>1578_bases
ATGGCAAAAAATGTCTCAAACGTCTCCCAAAACGAAGAGGTAACCAGCATCAGGAGGGTTATTACCGCATCCTCCGTTGG
TACGCTCATCGAATGGTATGACTTTTACATTTTCGGAAGTCTTGCAAAGATTATTTCCGAGCAATTCTTTCCAAAGGACA
ACCCGACAGCCGCGCTGCTTGCCACCCTCGCTACCTTTGCGGCAGGTTTTGTGGTGCGACCTTTCGGCGCGCTGTTTTTC
GGTCGCCTCGGTGACCTGATCGGAAGAAAATACACCTTTCTGGTAACACTCATCATCATGGGTGGATCAACCTTTGCCAT
AGGCCTGGTTCCCGGTTACAAAACGATCGGGTTTGTAGCTCCGGCAATCGTCTTTGTTTTGAGACTGCTCCAGGGTCTTG
CCCTCGGAGGCGAATACGGCGGTGCGGCAACCTATGTTGCTGAACACTCTCCGTCTGATAAACGTGGCTTCTGGACAAGC
TTCATTCAGACAACTGCCACCATCGGTCTGTTTGTCTCTCTTGGCGTTATTCTCATTGTCCGCCAAACGCTTGGTGTCGA
AACCTTTACCGACTGGGGCTGGAGAATTCCGTTCCTTCTCTCGATTTTTCTTGTTGCCATTTCAGTGTACATCCGCATGA
AAATGTCGGAATCCCCTGCATTTGCAAAACTGAAAAAAGAGGGAAAAACCTCAACCAACCCTCTTGCCGAGAGCTTCAAG
AAAAAAGACAACCTGAAAATGGTTCTGCTTGCACTGCTTGGCGCGACGGCTGGTCAGGGTGTGGTCTGGTACACCGGACA
GTTCTATGCGCTCTCTTTTCTTCAGAACGCCTGTAATGTGGAGTTTGTGCAGAGCAACTACATCATCTCCATAGCGCTGC
TCATCGGAACACCGTTCTTTATCATTTTCGGAGCACTTTCCGACAAGATCGGGCGCAAGTACATCATGATGACCGGCATG
CTGATTGCCGTGGTAGCGTACAGACCTATCTATACCGTCATGTACGAAGAAGCCAATCTCAAGGCCAAAACCGAAATCAA
GGAGAAGACAACAACAGAGGTGGCGCTGGCACTGAAAGGGGCTGATTCCGTAAAAACAACCATAACAAAGAAAACCTTCG
AAGACGGAACAACCTACAAGGAGACCAAAAAGGAAACAACCCCTGTTGATCTCGAAAAGAAAGCCGAGCTGGCCGCTGCC
GGCAAGCTGAAACCTGAAACCAAGAAGGAAACAACCCTTTCAGAGAGTTCATACTATAAAATGATCGGACTTGTGCTTAT
TCAGGTTCTTTTCGTCACCATGGTTTACGGCCCGATTGCCGCGTTCCTTGTCGAGTTGTTCCCGACCAGGATACGTTACA
CCTCCATGTCGCTACCTTATCACATTGGTAACGGCGTGTTCGGCGGTCTTGTTCCGCTTATTTCAACCCGACTTGTCGAA
GCGACCCGTCCCGGTCCTGATCTGCCGCCTGCCGACCCGCTTGCCGGTCTATGGTATCCTATCATTATTGGCGCGGTATG
CTTCCTTATCGGCTCGATCTATATTTCGGGCAAAACCAATAACCTGGACATCGACTAA

Upstream 100 bases:

>100_bases
AAACGAGCACGAGTTCATTAAGTAAATCCGCTTGCTGCAGAGAGCAGCTTCACACAGTTTTTTCAACACTATTTCCGTTT
AACAATCAAGGAGAAGATGT

Downstream 100 bases:

>100_bases
TCAAGAACCATTAAACTCTATTTAATTATGTCTGCAATTAAAAAGGTTGCCGGAATACTCTGGGCACTCATGGGCGCAGG
GATCATTCCGCTGGCGATCA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MAKNVSNVSQNEEVTSIRRVITASSVGTLIEWYDFYIFGSLAKIISEQFFPKDNPTAALLATLATFAAGFVVRPFGALFF
GRLGDLIGRKYTFLVTLIIMGGSTFAIGLVPGYKTIGFVAPAIVFVLRLLQGLALGGEYGGAATYVAEHSPSDKRGFWTS
FIQTTATIGLFVSLGVILIVRQTLGVETFTDWGWRIPFLLSIFLVAISVYIRMKMSESPAFAKLKKEGKTSTNPLAESFK
KKDNLKMVLLALLGATAGQGVVWYTGQFYALSFLQNACNVEFVQSNYIISIALLIGTPFFIIFGALSDKIGRKYIMMTGM
LIAVVAYRPIYTVMYEEANLKAKTEIKEKTTTEVALALKGADSVKTTITKKTFEDGTTYKETKKETTPVDLEKKAELAAA
GKLKPETKKETTLSESSYYKMIGLVLIQVLFVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGNGVFGGLVPLISTRLVE
ATRPGPDLPPADPLAGLWYPIIIGAVCFLIGSIYISGKTNNLDID

Sequences:

>Translated_525_residues
MAKNVSNVSQNEEVTSIRRVITASSVGTLIEWYDFYIFGSLAKIISEQFFPKDNPTAALLATLATFAAGFVVRPFGALFF
GRLGDLIGRKYTFLVTLIIMGGSTFAIGLVPGYKTIGFVAPAIVFVLRLLQGLALGGEYGGAATYVAEHSPSDKRGFWTS
FIQTTATIGLFVSLGVILIVRQTLGVETFTDWGWRIPFLLSIFLVAISVYIRMKMSESPAFAKLKKEGKTSTNPLAESFK
KKDNLKMVLLALLGATAGQGVVWYTGQFYALSFLQNACNVEFVQSNYIISIALLIGTPFFIIFGALSDKIGRKYIMMTGM
LIAVVAYRPIYTVMYEEANLKAKTEIKEKTTTEVALALKGADSVKTTITKKTFEDGTTYKETKKETTPVDLEKKAELAAA
GKLKPETKKETTLSESSYYKMIGLVLIQVLFVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGNGVFGGLVPLISTRLVE
ATRPGPDLPPADPLAGLWYPIIIGAVCFLIGSIYISGKTNNLDID
>Mature_524_residues
AKNVSNVSQNEEVTSIRRVITASSVGTLIEWYDFYIFGSLAKIISEQFFPKDNPTAALLATLATFAAGFVVRPFGALFFG
RLGDLIGRKYTFLVTLIIMGGSTFAIGLVPGYKTIGFVAPAIVFVLRLLQGLALGGEYGGAATYVAEHSPSDKRGFWTSF
IQTTATIGLFVSLGVILIVRQTLGVETFTDWGWRIPFLLSIFLVAISVYIRMKMSESPAFAKLKKEGKTSTNPLAESFKK
KDNLKMVLLALLGATAGQGVVWYTGQFYALSFLQNACNVEFVQSNYIISIALLIGTPFFIIFGALSDKIGRKYIMMTGML
IAVVAYRPIYTVMYEEANLKAKTEIKEKTTTEVALALKGADSVKTTITKKTFEDGTTYKETKKETTPVDLEKKAELAAAG
KLKPETKKETTLSESSYYKMIGLVLIQVLFVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGNGVFGGLVPLISTRLVEA
TRPGPDLPPADPLAGLWYPIIIGAVCFLIGSIYISGKTNNLDID

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=317, Percent_Identity=36.2776025236593, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1788292, Length=334, Percent_Identity=32.0359281437126, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI1789941, Length=342, Percent_Identity=37.4269005847953, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1788942, Length=334, Percent_Identity=33.8323353293413, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87082231, Length=335, Percent_Identity=24.1791044776119, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 57414; Mature: 57283

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNVSNVSQNEEVTSIRRVITASSVGTLIEWYDFYIFGSLAKIISEQFFPKDNPTAALL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ATLATFAAGFVVRPFGALFFGRLGDLIGRKYTFLVTLIIMGGSTFAIGLVPGYKTIGFVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
PAIVFVLRLLQGLALGGEYGGAATYVAEHSPSDKRGFWTSFIQTTATIGLFVSLGVILIV
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQTLGVETFTDWGWRIPFLLSIFLVAISVYIRMKMSESPAFAKLKKEGKTSTNPLAESFK
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCHHHHHHH
KKDNLKMVLLALLGATAGQGVVWYTGQFYALSFLQNACNVEFVQSNYIISIALLIGTPFF
CCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCEEEHHHHCCHHH
IIFGALSDKIGRKYIMMTGMLIAVVAYRPIYTVMYEEANLKAKTEIKEKTTTEVALALKG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHEEECC
ADSVKTTITKKTFEDGTTYKETKKETTPVDLEKKAELAAAGKLKPETKKETTLSESSYYK
CCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCHHHHHH
MIGLVLIQVLFVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGNGVFGGLVPLISTRLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCCHHHHHHHHHHHHHHH
ATRPGPDLPPADPLAGLWYPIIIGAVCFLIGSIYISGKTNNLDID
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure 
AKNVSNVSQNEEVTSIRRVITASSVGTLIEWYDFYIFGSLAKIISEQFFPKDNPTAALL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ATLATFAAGFVVRPFGALFFGRLGDLIGRKYTFLVTLIIMGGSTFAIGLVPGYKTIGFVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
PAIVFVLRLLQGLALGGEYGGAATYVAEHSPSDKRGFWTSFIQTTATIGLFVSLGVILIV
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQTLGVETFTDWGWRIPFLLSIFLVAISVYIRMKMSESPAFAKLKKEGKTSTNPLAESFK
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCHHHHHHH
KKDNLKMVLLALLGATAGQGVVWYTGQFYALSFLQNACNVEFVQSNYIISIALLIGTPFF
CCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCEEEHHHHCCHHH
IIFGALSDKIGRKYIMMTGMLIAVVAYRPIYTVMYEEANLKAKTEIKEKTTTEVALALKG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHEEECC
ADSVKTTITKKTFEDGTTYKETKKETTPVDLEKKAELAAAGKLKPETKKETTLSESSYYK
CCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCHHHHHH
MIGLVLIQVLFVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGNGVFGGLVPLISTRLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCCHHHHHHHHHHHHHHH
ATRPGPDLPPADPLAGLWYPIIIGAVCFLIGSIYISGKTNNLDID
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]