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The map label for this gene is pcrA [H]

Identifier: 119356509

GI number: 119356509

Start: 769234

End: 771438

Strand: Direct

Name: pcrA [H]

Synonym: Cpha266_0674

Alternate gene names: 119356509

Gene position: 769234-771438 (Clockwise)

Preceding gene: 119356505

Following gene: 119356510

Centisome position: 24.55

GC content: 49.02

Gene sequence:

>2205_bases
TTGACGGATTTTTTACTTGATCTTAATGATGTTCAGCGCCAGGCGGTCAGAGCCACAGAGGGTCCTGTCATGGTGCTTGC
CGGAGCAGGTTCAGGCAAGACAAGGGTTATTACCTATCGCATTGCCTGGTTGATAAAGAACGGCTATGCTTCAGCAAAGA
ACATTCTTGCCCTTACTTTTACCAACAAGGCTGCCGGTGAAATGCGTCACCGTGTCGATGTTCTTTTGCAGCAGGGTTCA
GCCGGAGGATTGTGGATAGGTACCTTTCATTCGATTTTTGCGCGCCTGTTGCGCAATGATATTCATTTGCTCGGGTATGA
CAGGAACTTTTCGATATTTGATGCCGACGACAGTAAAAGTCTGATTCGCCAGTCGATGGGTGAGCTTAATATTTCGCCTG
ATTCCGTGCCGCTCAATACCCTGCAGGGAATCATCAGCAGAGCCAAAAACAGTTTTATTCTGCCCGAAGAGTTTCGCCGG
AGCGCAGGAGACTATAATCAGCAGATTGCCGCAAAGGTCTATGATCTTTACACCAGAAAGCTCAGGGAGAATAACGCCCT
TGATTTCGACGATCTGCTTATCAAGCCACTTGAACTGTTCAGGGAACACCCGATGGTGCTTGAGGCATTGCAGGATACCT
TTCGGTACCTGCTTATCGATGAGTACCAGGATACCAACAAGGCGCAGTATCTGGCCGCAAAAATGCTCTCGGCAAAGCAT
CGCAACATTTTTGTTGTCGGTGATGACGCCCAGTCTATTTATTCCTGGCGAGGGGCGGATATATCCAATATTCTGAACTT
TCAGGATGATTACACGGATGCTCTGACCTTCAAGCTGGTGGAGAATTACCGGAGTACCGGAACGATTCTTCAGGCAGCCA
ACAGCGTGATTCGAAACAACCTTCGCCAGATAAAAAAAGAGCTCGTTTCGCATCGAAGCGCGGGCGATCCCCTGACGCTT
ATCGAGGCTCGAAATGAACGGCATGAGGCTGAAAAGATCGGGGAGTACATCCACTCGATCCGCATTGAAAAAGGGTACGA
ATTTCGGAGTTTCGCAGTGTTTTACCGAACAAATGCCCAGTCGAGGGTGCTTGAGGATCTCATGCGACAGAACCGGATTC
CCTACAAGATATTCGGGAGCGTCTCGTTTTACAAACGTAAGGAGATCAAGGATGCGGTTGCCTATCTCCGCTTTCTTCTC
AATGATCGGGACAGTGAGTCACTTTTGAGAATTATTAATTTTCCGCCCAGAAAAATCGGTGAAACAAGTATCGGAAAACT
ACGGGAGTTTGCTGAAGCAGAGGGTATAACGCTGTACGAGGCAATAACCCGCGCAGATGAAGGGCAGTTCCAGTCTCGTC
TTGTAAACTCGCTCAAGGGCTTCAGTTCGCTTATTGAAGCCTTGCGTCTGCTCGCCGAGACGGGTACGGTCTATGCTGTA
TTGACGGAACTGTTCAGTTTGACCTCGATTCCTCTTCTGCTCAAGGAGGAAAATACCGCAGAATCTCTTGCGAGGCATGA
AAATCTTCAGGAACTGCTCTCGATGGCAAGGGATTTTTCGGATAACAATCCTGATCAAGGTTCTCTCGGTGATTTTCTTG
AAACAATATCGCTGGCCTCCAACTACGACGAGTCTCAGGATTCCGATAACTATGTTTCACTGATGACGGTTCACGCATCG
AAGGGACTTGAGTTTCCCGTTGTATTTATAACCGGACTTGAAGAGCGGCTTTTTCCCCTGAACTGTTATGAACCTGAACA
GCTTGAGGAGGAACGGCGGTTGTTTTATGTTGCCATGACCAGAGCCCAGGAAAAGATTTTTCTTTCATGGGCACAGACCC
GTTATCTCTATGGTCAGCCGCAGTACTGTCTGAAATCGATGTTTATCAGCGAGATTGATTCTTCAATCGTCAAGACTGAA
AGCGGAACTGTTCTTGCAGACAGAAAAGAGCGGCAGGGTACCCGGAACGCCGTTCCGGAATCCGGCGGTTATCGTCAAAG
ACCCGCGGGAGCAGTTTCCGCTCCTTCTCAGAAGCCCCGGGAAAGCGGACTTCGAGAAGGCTCGACGGTGCATCATGCGG
TTTTCGGCCAGGGGACGGTGCTTGATGTCCAGGGTCGAGGTGCAGGCCAGAAAGCGCTTATCAGGTTTCGCAATGCAGGC
GAGAAAACGTTGATGGTTCAGTATGCAAATCTCAGAGTGTTATGA

Upstream 100 bases:

>100_bases
TTCGGACAAACTTATACGAAATTCAACAGGAGCATTTCAGCGCTTTTGCTGCACGGTGTCGTTCGAGACACTGAAAACGG
TTAACAGGTATCCAGGAGTT

Downstream 100 bases:

>100_bases
TCAATTGGTTGTTTTTGATGGATAAAAGTTCAGGAATATGAAGATTCTTTTCGGTGTTCAGGGTACAGGAAACGGCCATA
TCAGCCGCAGCAGAGAGTTG

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MTDFLLDLNDVQRQAVRATEGPVMVLAGAGSGKTRVITYRIAWLIKNGYASAKNILALTFTNKAAGEMRHRVDVLLQQGS
AGGLWIGTFHSIFARLLRNDIHLLGYDRNFSIFDADDSKSLIRQSMGELNISPDSVPLNTLQGIISRAKNSFILPEEFRR
SAGDYNQQIAAKVYDLYTRKLRENNALDFDDLLIKPLELFREHPMVLEALQDTFRYLLIDEYQDTNKAQYLAAKMLSAKH
RNIFVVGDDAQSIYSWRGADISNILNFQDDYTDALTFKLVENYRSTGTILQAANSVIRNNLRQIKKELVSHRSAGDPLTL
IEARNERHEAEKIGEYIHSIRIEKGYEFRSFAVFYRTNAQSRVLEDLMRQNRIPYKIFGSVSFYKRKEIKDAVAYLRFLL
NDRDSESLLRIINFPPRKIGETSIGKLREFAEAEGITLYEAITRADEGQFQSRLVNSLKGFSSLIEALRLLAETGTVYAV
LTELFSLTSIPLLLKEENTAESLARHENLQELLSMARDFSDNNPDQGSLGDFLETISLASNYDESQDSDNYVSLMTVHAS
KGLEFPVVFITGLEERLFPLNCYEPEQLEEERRLFYVAMTRAQEKIFLSWAQTRYLYGQPQYCLKSMFISEIDSSIVKTE
SGTVLADRKERQGTRNAVPESGGYRQRPAGAVSAPSQKPRESGLREGSTVHHAVFGQGTVLDVQGRGAGQKALIRFRNAG
EKTLMVQYANLRVL

Sequences:

>Translated_734_residues
MTDFLLDLNDVQRQAVRATEGPVMVLAGAGSGKTRVITYRIAWLIKNGYASAKNILALTFTNKAAGEMRHRVDVLLQQGS
AGGLWIGTFHSIFARLLRNDIHLLGYDRNFSIFDADDSKSLIRQSMGELNISPDSVPLNTLQGIISRAKNSFILPEEFRR
SAGDYNQQIAAKVYDLYTRKLRENNALDFDDLLIKPLELFREHPMVLEALQDTFRYLLIDEYQDTNKAQYLAAKMLSAKH
RNIFVVGDDAQSIYSWRGADISNILNFQDDYTDALTFKLVENYRSTGTILQAANSVIRNNLRQIKKELVSHRSAGDPLTL
IEARNERHEAEKIGEYIHSIRIEKGYEFRSFAVFYRTNAQSRVLEDLMRQNRIPYKIFGSVSFYKRKEIKDAVAYLRFLL
NDRDSESLLRIINFPPRKIGETSIGKLREFAEAEGITLYEAITRADEGQFQSRLVNSLKGFSSLIEALRLLAETGTVYAV
LTELFSLTSIPLLLKEENTAESLARHENLQELLSMARDFSDNNPDQGSLGDFLETISLASNYDESQDSDNYVSLMTVHAS
KGLEFPVVFITGLEERLFPLNCYEPEQLEEERRLFYVAMTRAQEKIFLSWAQTRYLYGQPQYCLKSMFISEIDSSIVKTE
SGTVLADRKERQGTRNAVPESGGYRQRPAGAVSAPSQKPRESGLREGSTVHHAVFGQGTVLDVQGRGAGQKALIRFRNAG
EKTLMVQYANLRVL
>Mature_733_residues
TDFLLDLNDVQRQAVRATEGPVMVLAGAGSGKTRVITYRIAWLIKNGYASAKNILALTFTNKAAGEMRHRVDVLLQQGSA
GGLWIGTFHSIFARLLRNDIHLLGYDRNFSIFDADDSKSLIRQSMGELNISPDSVPLNTLQGIISRAKNSFILPEEFRRS
AGDYNQQIAAKVYDLYTRKLRENNALDFDDLLIKPLELFREHPMVLEALQDTFRYLLIDEYQDTNKAQYLAAKMLSAKHR
NIFVVGDDAQSIYSWRGADISNILNFQDDYTDALTFKLVENYRSTGTILQAANSVIRNNLRQIKKELVSHRSAGDPLTLI
EARNERHEAEKIGEYIHSIRIEKGYEFRSFAVFYRTNAQSRVLEDLMRQNRIPYKIFGSVSFYKRKEIKDAVAYLRFLLN
DRDSESLLRIINFPPRKIGETSIGKLREFAEAEGITLYEAITRADEGQFQSRLVNSLKGFSSLIEALRLLAETGTVYAVL
TELFSLTSIPLLLKEENTAESLARHENLQELLSMARDFSDNNPDQGSLGDFLETISLASNYDESQDSDNYVSLMTVHASK
GLEFPVVFITGLEERLFPLNCYEPEQLEEERRLFYVAMTRAQEKIFLSWAQTRYLYGQPQYCLKSMFISEIDSSIVKTES
GTVLADRKERQGTRNAVPESGGYRQRPAGAVSAPSQKPRESGLREGSTVHHAVFGQGTVLDVQGRGAGQKALIRFRNAGE
KTLMVQYANLRVL

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=742, Percent_Identity=36.2533692722372, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI48994965, Length=660, Percent_Identity=38.6363636363636, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=343, Percent_Identity=27.9883381924198, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=729, Percent_Identity=27.0233196159122, Blast_Score=193, Evalue=1e-49,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83184; Mature: 83053

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLLDLNDVQRQAVRATEGPVMVLAGAGSGKTRVITYRIAWLIKNGYASAKNILALTF
CCCCEECHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEEEHHHCCCCCCCCEEEEEE
TNKAAGEMRHRVDVLLQQGSAGGLWIGTFHSIFARLLRNDIHLLGYDRNFSIFDADDSKS
CCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHH
LIRQSMGELNISPDSVPLNTLQGIISRAKNSFILPEEFRRSAGDYNQQIAAKVYDLYTRK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
LRENNALDFDDLLIKPLELFREHPMVLEALQDTFRYLLIDEYQDTNKAQYLAAKMLSAKH
HHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
RNIFVVGDDAQSIYSWRGADISNILNFQDDYTDALTFKLVENYRSTGTILQAANSVIRNN
CEEEEEECCHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQIKKELVSHRSAGDPLTLIEARNERHEAEKIGEYIHSIRIEKGYEFRSFAVFYRTNAQ
HHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCH
SRVLEDLMRQNRIPYKIFGSVSFYKRKEIKDAVAYLRFLLNDRDSESLLRIINFPPRKIG
HHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCC
ETSIGKLREFAEAEGITLYEAITRADEGQFQSRLVNSLKGFSSLIEALRLLAETGTVYAV
CCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LTELFSLTSIPLLLKEENTAESLARHENLQELLSMARDFSDNNPDQGSLGDFLETISLAS
HHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
NYDESQDSDNYVSLMTVHASKGLEFPVVFITGLEERLFPLNCYEPEQLEEERRLFYVAMT
CCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHEEHHHHH
RAQEKIFLSWAQTRYLYGQPQYCLKSMFISEIDSSIVKTESGTVLADRKERQGTRNAVPE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCC
SGGYRQRPAGAVSAPSQKPRESGLREGSTVHHAVFGQGTVLDVQGRGAGQKALIRFRNAG
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCC
EKTLMVQYANLRVL
CEEEEEEECCEEEC
>Mature Secondary Structure 
TDFLLDLNDVQRQAVRATEGPVMVLAGAGSGKTRVITYRIAWLIKNGYASAKNILALTF
CCCEECHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEEEHHHCCCCCCCCEEEEEE
TNKAAGEMRHRVDVLLQQGSAGGLWIGTFHSIFARLLRNDIHLLGYDRNFSIFDADDSKS
CCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHH
LIRQSMGELNISPDSVPLNTLQGIISRAKNSFILPEEFRRSAGDYNQQIAAKVYDLYTRK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
LRENNALDFDDLLIKPLELFREHPMVLEALQDTFRYLLIDEYQDTNKAQYLAAKMLSAKH
HHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
RNIFVVGDDAQSIYSWRGADISNILNFQDDYTDALTFKLVENYRSTGTILQAANSVIRNN
CEEEEEECCHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQIKKELVSHRSAGDPLTLIEARNERHEAEKIGEYIHSIRIEKGYEFRSFAVFYRTNAQ
HHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCH
SRVLEDLMRQNRIPYKIFGSVSFYKRKEIKDAVAYLRFLLNDRDSESLLRIINFPPRKIG
HHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCC
ETSIGKLREFAEAEGITLYEAITRADEGQFQSRLVNSLKGFSSLIEALRLLAETGTVYAV
CCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LTELFSLTSIPLLLKEENTAESLARHENLQELLSMARDFSDNNPDQGSLGDFLETISLAS
HHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
NYDESQDSDNYVSLMTVHASKGLEFPVVFITGLEERLFPLNCYEPEQLEEERRLFYVAMT
CCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHEEHHHHH
RAQEKIFLSWAQTRYLYGQPQYCLKSMFISEIDSSIVKTESGTVLADRKERQGTRNAVPE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCC
SGGYRQRPAGAVSAPSQKPRESGLREGSTVHHAVFGQGTVLDVQGRGAGQKALIRFRNAG
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCC
EKTLMVQYANLRVL
CEEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]