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The map label for this gene is gltD [H]

Identifier: 119356464

GI number: 119356464

Start: 710095

End: 711570

Strand: Direct

Name: gltD [H]

Synonym: Cpha266_0629

Alternate gene names: 119356464

Gene position: 710095-711570 (Clockwise)

Preceding gene: 119356463

Following gene: 119356465

Centisome position: 22.66

GC content: 51.29

Gene sequence:

>1476_bases
ATGGGAAAAGTTAAAGGTTTTATGGAGTTTCAGCGGGTAGTTCCCTCTGATAGCAAGCCTCTTGAAAGGTTAAAGGATTG
GCAGGAGTTTCATGAGGAAATGCCGGTTTCAGCATTGCGGGAGCAGGGCGCGCGCTGTATGGATTGCGGCACCCCCTATT
GTCATACCGGGTTTATGCTGAGCGGTATGGCGAGCGGATGCCCGATACACAATCTCATACCCGAGTGGAACGATTATGTA
TATAACGGCTTGTGGAAAGACGCCTATGAACGGCTCATGAAAACCAACAATTTCCCTGAATTTACCGGCAGAGTCTGTCC
GGCACCTTGTGAAGGTTCCTGTGTGCTCGGCATTATCAAGCCGCCGGTTACTATCAAGAATATCGAGTGTTCGATTATCG
AACATGCGTTTGCGGAAGGGTGGGTTGAGCCGAAAATAATCGCTTATAGAACGGGAAAGCGTGTTGCGGTAATCGGATCC
GGGCCTGCCGGACTTGCCTGCGCCGATCAGCTCAACAAAGTTGGCCATAGCGTTACCGTATTCGAACGAGACGATCGTAT
CGGTGGTCTCATGATGTACGGCATTCCTAACATGAAGCTCGATAAGGAGCTGGTTGTTCAGCGTCGCGTCAATATGATGA
AGAGTGAAGGCATTGTTTTTATGGAAAGTACCGAGGTGGGTGTTGACTATCCTGCTGACAAACTGCTTGAAGAGTTTGAC
GCCGTCGTTCTCTGCACCGGAGCAACAAGACCTCGAGATCTTGAGGTGGAAGGACGAGCACTCTCGGGAGTCAATTATGC
AATGGATTTCCTTCGTTCGAGTACGAAATCTGTTCTCGCGTCGACACCTCCGGACATTTCTGCTGTCGGAAAGGATGTGA
TTGTCATCGGTGGCGGTGATACCGGTACGGACTGCGTTGCCTCTTCGTTGCGTCAGGGATGCAGGAGCGTTATGCAGCTT
GAGATTATGCCGAAACCTCCGCTTGAGAGGCAGTTGGATAATCCATGGCCGGAGTGGCCGAAAACCTTCAAGGTTGATTA
CGGCCAGGAGGAAGCTGCTGCTGTGCAGGGTGAGGATCCCCGTAAGTACGCCATGATGACCAAGAAATTTCTTTCAGACG
ACCAGGGTGAGCTTTGTGCTGTTGAAGTTTCAAGTGTAGAGTGGGTGAACGTTGACGGACGTTTTGTCCCGCAGCCACTT
TCAGGCACTGAACAGATTTACCCTGCGCAGCTTGTGCTGCTCGCGATGGGATTTGTTGGCCCTGAAGAGCATCTTCTTCA
GGCGATGCAGATTCTTCAGGATGAACGTTCCAACATCAAGGCAGATGAAAAGAGTTATCGTACCAGTCGCGAAAGGATTT
TTGCGGCAGGTGATGCCCGTCGCGGCCAGAGTCTTGTTGTCTGGGCAATCAATGAAGGTCGGGGTGCCGCACGTGAATGC
GACCGCTTTCTTATGGGCCAGACAAGCTTGCCCTAA

Upstream 100 bases:

>100_bases
GCCGCTTTTGAAAAGAATGTTCACGACCCTGCGCGTGTTTCAGGGAATTAATTCCGGAAAGGCGTATTCGCCTTGTTCGG
TAAAGCTTTAAACGATTGCT

Downstream 100 bases:

>100_bases
ACGTTTCACTCTCGGTAAGTGTTATAGAGAAAACAAGTACAGACAGACCGCTCTGAAAATGGTTGATGGTAATGGTTTTT
TGCAGCGGTCTGTTACAATA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MGKVKGFMEFQRVVPSDSKPLERLKDWQEFHEEMPVSALREQGARCMDCGTPYCHTGFMLSGMASGCPIHNLIPEWNDYV
YNGLWKDAYERLMKTNNFPEFTGRVCPAPCEGSCVLGIIKPPVTIKNIECSIIEHAFAEGWVEPKIIAYRTGKRVAVIGS
GPAGLACADQLNKVGHSVTVFERDDRIGGLMMYGIPNMKLDKELVVQRRVNMMKSEGIVFMESTEVGVDYPADKLLEEFD
AVVLCTGATRPRDLEVEGRALSGVNYAMDFLRSSTKSVLASTPPDISAVGKDVIVIGGGDTGTDCVASSLRQGCRSVMQL
EIMPKPPLERQLDNPWPEWPKTFKVDYGQEEAAAVQGEDPRKYAMMTKKFLSDDQGELCAVEVSSVEWVNVDGRFVPQPL
SGTEQIYPAQLVLLAMGFVGPEEHLLQAMQILQDERSNIKADEKSYRTSRERIFAAGDARRGQSLVVWAINEGRGAAREC
DRFLMGQTSLP

Sequences:

>Translated_491_residues
MGKVKGFMEFQRVVPSDSKPLERLKDWQEFHEEMPVSALREQGARCMDCGTPYCHTGFMLSGMASGCPIHNLIPEWNDYV
YNGLWKDAYERLMKTNNFPEFTGRVCPAPCEGSCVLGIIKPPVTIKNIECSIIEHAFAEGWVEPKIIAYRTGKRVAVIGS
GPAGLACADQLNKVGHSVTVFERDDRIGGLMMYGIPNMKLDKELVVQRRVNMMKSEGIVFMESTEVGVDYPADKLLEEFD
AVVLCTGATRPRDLEVEGRALSGVNYAMDFLRSSTKSVLASTPPDISAVGKDVIVIGGGDTGTDCVASSLRQGCRSVMQL
EIMPKPPLERQLDNPWPEWPKTFKVDYGQEEAAAVQGEDPRKYAMMTKKFLSDDQGELCAVEVSSVEWVNVDGRFVPQPL
SGTEQIYPAQLVLLAMGFVGPEEHLLQAMQILQDERSNIKADEKSYRTSRERIFAAGDARRGQSLVVWAINEGRGAAREC
DRFLMGQTSLP
>Mature_490_residues
GKVKGFMEFQRVVPSDSKPLERLKDWQEFHEEMPVSALREQGARCMDCGTPYCHTGFMLSGMASGCPIHNLIPEWNDYVY
NGLWKDAYERLMKTNNFPEFTGRVCPAPCEGSCVLGIIKPPVTIKNIECSIIEHAFAEGWVEPKIIAYRTGKRVAVIGSG
PAGLACADQLNKVGHSVTVFERDDRIGGLMMYGIPNMKLDKELVVQRRVNMMKSEGIVFMESTEVGVDYPADKLLEEFDA
VVLCTGATRPRDLEVEGRALSGVNYAMDFLRSSTKSVLASTPPDISAVGKDVIVIGGGDTGTDCVASSLRQGCRSVMQLE
IMPKPPLERQLDNPWPEWPKTFKVDYGQEEAAAVQGEDPRKYAMMTKKFLSDDQGELCAVEVSSVEWVNVDGRFVPQPLS
GTEQIYPAQLVLLAMGFVGPEEHLLQAMQILQDERSNIKADEKSYRTSRERIFAAGDARRGQSLVVWAINEGRGAARECD
RFLMGQTSLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=477, Percent_Identity=23.2704402515723, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=36.530612244898, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=35.7601713062098, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082180, Length=424, Percent_Identity=33.9622641509434, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=25.8849557522124, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789243, Length=467, Percent_Identity=26.5524625267666, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17570289, Length=501, Percent_Identity=50.0998003992016, Blast_Score=511, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71984108, Length=483, Percent_Identity=22.360248447205, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=30.4597701149425, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=494, Percent_Identity=48.1781376518219, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI28574881, Length=491, Percent_Identity=53.1568228105906, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24665539, Length=491, Percent_Identity=53.1568228105906, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24665547, Length=491, Percent_Identity=53.1568228105906, Blast_Score=549, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24665543, Length=491, Percent_Identity=53.1568228105906, Blast_Score=549, Evalue=1e-156,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54440; Mature: 54309

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVKGFMEFQRVVPSDSKPLERLKDWQEFHEEMPVSALREQGARCMDCGTPYCHTGFML
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEECCCCCCHHHHHHH
SGMASGCPIHNLIPEWNDYVYNGLWKDAYERLMKTNNFPEFTGRVCPAPCEGSCVLGIIK
HCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEEEC
PPVTIKNIECSIIEHAFAEGWVEPKIIAYRTGKRVAVIGSGPAGLACADQLNKVGHSVTV
CCEEEECCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEE
FERDDRIGGLMMYGIPNMKLDKELVVQRRVNMMKSEGIVFMESTEVGVDYPADKLLEEFD
EECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCC
AVVLCTGATRPRDLEVEGRALSGVNYAMDFLRSSTKSVLASTPPDISAVGKDVIVIGGGD
EEEEEECCCCCCCEEECCCEECCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECCC
TGTDCVASSLRQGCRSVMQLEIMPKPPLERQLDNPWPEWPKTFKVDYGQEEAAAVQGEDP
CCHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCHHHHCCCCCC
RKYAMMTKKFLSDDQGELCAVEVSSVEWVNVDGRFVPQPLSGTEQIYPAQLVLLAMGFVG
HHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PEEHLLQAMQILQDERSNIKADEKSYRTSRERIFAAGDARRGQSLVVWAINEGRGAAREC
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCCCCCCCEEEEEEEECCCCCHHHH
DRFLMGQTSLP
HHHHHCCCCCC
>Mature Secondary Structure 
GKVKGFMEFQRVVPSDSKPLERLKDWQEFHEEMPVSALREQGARCMDCGTPYCHTGFML
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEECCCCCCHHHHHHH
SGMASGCPIHNLIPEWNDYVYNGLWKDAYERLMKTNNFPEFTGRVCPAPCEGSCVLGIIK
HCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEEEC
PPVTIKNIECSIIEHAFAEGWVEPKIIAYRTGKRVAVIGSGPAGLACADQLNKVGHSVTV
CCEEEECCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEE
FERDDRIGGLMMYGIPNMKLDKELVVQRRVNMMKSEGIVFMESTEVGVDYPADKLLEEFD
EECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCC
AVVLCTGATRPRDLEVEGRALSGVNYAMDFLRSSTKSVLASTPPDISAVGKDVIVIGGGD
EEEEEECCCCCCCEEECCCEECCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECCC
TGTDCVASSLRQGCRSVMQLEIMPKPPLERQLDNPWPEWPKTFKVDYGQEEAAAVQGEDP
CCHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCHHHHCCCCCC
RKYAMMTKKFLSDDQGELCAVEVSSVEWVNVDGRFVPQPLSGTEQIYPAQLVLLAMGFVG
HHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PEEHLLQAMQILQDERSNIKADEKSYRTSRERIFAAGDARRGQSLVVWAINEGRGAAREC
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCCCCCCCEEEEEEEECCCCCHHHH
DRFLMGQTSLP
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]