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The map label for this gene is tilS [H]

Identifier: 119356415

GI number: 119356415

Start: 650316

End: 651293

Strand: Direct

Name: tilS [H]

Synonym: Cpha266_0579

Alternate gene names: 119356415

Gene position: 650316-651293 (Clockwise)

Preceding gene: 119356410

Following gene: 119356421

Centisome position: 20.75

GC content: 47.24

Gene sequence:

>978_bases
GTGAATGTACTTGAGAAGAAGTTTCTTGAACAGATCCGTATTCGCCAGCTTGTTTCGAAAGAGGATCATGTTCTGCTTGC
GGTATCGGGTGGATCGGATTCGATGGCCCTTCTTCATCTGTTTTGCGCAATCCGGAGGGCCGTTCCGTGCAGGATAAGCG
CTGTTCATTGTAATTTCAGGCTTCGAGCCGAAGAGAGCGATGAGGACGAGCGTTTTGTTCAGGAAGCCTGCCTCGCTCTT
GGCGTTACCTGTTATACTGTTGGTTTTGATACGCTGAAGATGGCCGGCGAGTGGAAAACCTCAATCGAGGAAACTGCGCG
TATACTCCGGTATCGATATTTCGATACCCTGTTGAGTGAGCATGGGATGAAGGTTGTTGCCACCGGGCATCATGTGAATG
ATAATGCTGAAACGATGTTGTTCAATCTTTTCAGGGGAACCTCTCTGCTTGGGCTCAGAGGTATTCGCGCAAAACGCGGA
AAGATCATTCGTCCGCTGCTGCTTGTTCATAAAGTTGAAATATTGGGGTACATCGATGAAAAGCATATTGCATTCCGTAT
CGATGCGACCAATTTTGGTATAGATCCTGACAGGAATTTTATTCGCAACAGGATAATTCCGCTTATTGAAGAGCGGTTTA
GTCATAAGCTGCTTCCCTCCCTTCAGCGTCTTTCGGAACAGGCAGGAGAACTTGAGGAATTTCTTGAGCTCTATTTCGAG
AATCTTCTTGCTGATAAACCGGGACTATCGGCATCAAACGGGATGCTTGAGGTAAAGGATCTTGTGCTTCTGAGTTTGTT
CGAGCAAAAAGAGGTATTCAAAAGAGCGTTGCGTGAACATGATGTCAAATGTGATTCGAAAACGCTGGACCAGCTTGTCC
GGCTGCTGAAAACTCAGCCGGGAAGAATGGTCAGGCTCAGTGCACAGATTGTTGTGCTCTGGAAAGGGGGGACGCTTCAT
ATTTTACGCCAGAGTTAG

Upstream 100 bases:

>100_bases
TTTTTAGTGCTCATATCCAGCGCGTTTATTACCTTTGGACTAAATAATACATTTTATTGTGCAGGTGTGCAACGTCAAAC
AGGTTAACTGGCATGAAAGA

Downstream 100 bases:

>100_bases
TTCTCCTTGCAACCATGAAGTTTTTTTTTATAGAAGAACTCTCGATCGTTCGGGGAAGAGCACTATTTATTTGCCGGTGT
TTTCCTGAAGATCTCTGGCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNVLEKKFLEQIRIRQLVSKEDHVLLAVSGGSDSMALLHLFCAIRRAVPCRISAVHCNFRLRAEESDEDERFVQEACLAL
GVTCYTVGFDTLKMAGEWKTSIEETARILRYRYFDTLLSEHGMKVVATGHHVNDNAETMLFNLFRGTSLLGLRGIRAKRG
KIIRPLLLVHKVEILGYIDEKHIAFRIDATNFGIDPDRNFIRNRIIPLIEERFSHKLLPSLQRLSEQAGELEEFLELYFE
NLLADKPGLSASNGMLEVKDLVLLSLFEQKEVFKRALREHDVKCDSKTLDQLVRLLKTQPGRMVRLSAQIVVLWKGGTLH
ILRQS

Sequences:

>Translated_325_residues
MNVLEKKFLEQIRIRQLVSKEDHVLLAVSGGSDSMALLHLFCAIRRAVPCRISAVHCNFRLRAEESDEDERFVQEACLAL
GVTCYTVGFDTLKMAGEWKTSIEETARILRYRYFDTLLSEHGMKVVATGHHVNDNAETMLFNLFRGTSLLGLRGIRAKRG
KIIRPLLLVHKVEILGYIDEKHIAFRIDATNFGIDPDRNFIRNRIIPLIEERFSHKLLPSLQRLSEQAGELEEFLELYFE
NLLADKPGLSASNGMLEVKDLVLLSLFEQKEVFKRALREHDVKCDSKTLDQLVRLLKTQPGRMVRLSAQIVVLWKGGTLH
ILRQS
>Mature_325_residues
MNVLEKKFLEQIRIRQLVSKEDHVLLAVSGGSDSMALLHLFCAIRRAVPCRISAVHCNFRLRAEESDEDERFVQEACLAL
GVTCYTVGFDTLKMAGEWKTSIEETARILRYRYFDTLLSEHGMKVVATGHHVNDNAETMLFNLFRGTSLLGLRGIRAKRG
KIIRPLLLVHKVEILGYIDEKHIAFRIDATNFGIDPDRNFIRNRIIPLIEERFSHKLLPSLQRLSEQAGELEEFLELYFE
NLLADKPGLSASNGMLEVKDLVLLSLFEQKEVFKRALREHDVKCDSKTLDQLVRLLKTQPGRMVRLSAQIVVLWKGGTLH
ILRQS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=289, Percent_Identity=27.681660899654, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 37222; Mature: 37222

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLEKKFLEQIRIRQLVSKEDHVLLAVSGGSDSMALLHLFCAIRRAVPCRISAVHCNFR
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEE
LRAEESDEDERFVQEACLALGVTCYTVGFDTLKMAGEWKTSIEETARILRYRYFDTLLSE
EECCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
HGMKVVATGHHVNDNAETMLFNLFRGTSLLGLRGIRAKRGKIIRPLLLVHKVEILGYIDE
CCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHCCCCHHCCCHHHHHHHHHHHHHHHEECC
KHIAFRIDATNFGIDPDRNFIRNRIIPLIEERFSHKLLPSLQRLSEQAGELEEFLELYFE
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLADKPGLSASNGMLEVKDLVLLSLFEQKEVFKRALREHDVKCDSKTLDQLVRLLKTQP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GRMVRLSAQIVVLWKGGTLHILRQS
CCEEEEEEEEEEEEECCEEEEEECC
>Mature Secondary Structure
MNVLEKKFLEQIRIRQLVSKEDHVLLAVSGGSDSMALLHLFCAIRRAVPCRISAVHCNFR
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEE
LRAEESDEDERFVQEACLALGVTCYTVGFDTLKMAGEWKTSIEETARILRYRYFDTLLSE
EECCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
HGMKVVATGHHVNDNAETMLFNLFRGTSLLGLRGIRAKRGKIIRPLLLVHKVEILGYIDE
CCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHCCCCHHCCCHHHHHHHHHHHHHHHEECC
KHIAFRIDATNFGIDPDRNFIRNRIIPLIEERFSHKLLPSLQRLSEQAGELEEFLELYFE
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLADKPGLSASNGMLEVKDLVLLSLFEQKEVFKRALREHDVKCDSKTLDQLVRLLKTQP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GRMVRLSAQIVVLWKGGTLHILRQS
CCEEEEEEEEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10976061; 12093901 [H]