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The map label for this gene is purD [H]

Identifier: 119356365

GI number: 119356365

Start: 581100

End: 582383

Strand: Direct

Name: purD [H]

Synonym: Cpha266_0528

Alternate gene names: 119356365

Gene position: 581100-582383 (Clockwise)

Preceding gene: 119356362

Following gene: 119356366

Centisome position: 18.54

GC content: 51.01

Gene sequence:

>1284_bases
ATGAACGTATTGATAATAGGGAGTGGTGCCCGCGAGCATGCCATGGCATGGGCTGCCGCCAGGAACGATGCGGTGCAACA
GGTCTATGTCGCACCGGGAAACGGCGGTACAGCGCTCATGGGGGGAAAGGTTCAAAACGTTGCGCTCAAAGCCACTGCGC
TTGACGAGCTGCTTGAGTTTGCAGTTTTAAAGGCGGTTGATCTGACGATTGCCGGTTCTGAACAACCGCTTGAACTTGGT
ATTGTCGATCTGTTTCGATCGGCACAAAAAAAGATTATCGGTCCTTCGCAGGCTGCTGCGCAATTGGAGACAAGCAAGGT
GTTCGCCAAGGATTTTATGTCGCGTTACGGGATACCTACGGCCGGTTATCATGTTTTCAGGGAGTTTCAGACGGCATCAC
TCTATCTCGCTTCGCTTGACGAATCAAGATATCCCCAGGTGATCAAGGCAAGCGGCTTGTGTGCAGGCAAGGGTGTTATT
GTCGCTTCTGACGGAATGGAGGCTTTTCGTGCGACTCGTGCCATGTTTGAGGATCGCGTGTTTGGCGATGCTGCCGACGA
GGTTGTCATAGAGGATTTTCTGGAGGGCGAGGAGGCAAGTGTTTTCGTTCTGACGGATGGCGAAAAATACCGGATGTTTT
ATTCGGCCCAGGATCATAAGCGGATCGGGGAGGGAGATACCGGAAAAAATACAGGAGGGATGGGTGCTTACGCTCCTGCG
CCTCTGGTGACTTCCGGGGTTATGAAAAAAATTGAGGAGCAGATCGTTGTTCCTGCTCTTGAAGGAATGGCGGCTGATGG
AACTCCCTATACCGGCTTTCTCTACGTGGGCGTCATGATCGATAAGGGCGAACCCTCTGTTGTTGAGTTCAATGCCCGAA
TGGGAGATCCCGAAACGCAGGTTGTTCTGCCTCTGTTGAAAGATGATTTTATCGATGCACTTCAGGCAAGTCTTCAGGGT
ACTCTGGATCTTGTGCCGTTTGAGATGCTCGAGCAGTGCGCAACGACGGTGGTTATCGCTTCGAAAGGGTATCCGGATCT
CTATGAAACCGGCAGGGAGATCACGATTGATAGTGAGCTTTTTGATATGCGTGATGTGTTGCTTTTTCATGCAGGGACCG
AGATTGAAAAAGGCACGCTCAGAACGTCGGGCGGCAGGGTTTTTTCGGTGACCGCTCTTGGCAAAACACTTATGGATAGT
ATTGAACTTGCATACAGGGCTGTTGATCATATCAGTTTTGAAGGGGCTTGCTTTCGTCGGGATATAGGGGCTAAGGCACT
TTAA

Upstream 100 bases:

>100_bases
TTTATATACAGGTTGTTGTCGCTTGACCATAACATATAATGTAACAAACCGGGTCGATAAACCGCAGGGTGAATCGCTGC
CAGTACATCGTGCAGGAGCA

Downstream 100 bases:

>100_bases
ATTATTCAGTTTAAAACATTCCATGAAGTTACTCCGGCGTCATTTTGAAATCATTCAGGAGCAGGCATTCCGCGAACAGC
CTTATGAGTGTTGCGGACTG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNVLIIGSGAREHAMAWAAARNDAVQQVYVAPGNGGTALMGGKVQNVALKATALDELLEFAVLKAVDLTIAGSEQPLELG
IVDLFRSAQKKIIGPSQAAAQLETSKVFAKDFMSRYGIPTAGYHVFREFQTASLYLASLDESRYPQVIKASGLCAGKGVI
VASDGMEAFRATRAMFEDRVFGDAADEVVIEDFLEGEEASVFVLTDGEKYRMFYSAQDHKRIGEGDTGKNTGGMGAYAPA
PLVTSGVMKKIEEQIVVPALEGMAADGTPYTGFLYVGVMIDKGEPSVVEFNARMGDPETQVVLPLLKDDFIDALQASLQG
TLDLVPFEMLEQCATTVVIASKGYPDLYETGREITIDSELFDMRDVLLFHAGTEIEKGTLRTSGGRVFSVTALGKTLMDS
IELAYRAVDHISFEGACFRRDIGAKAL

Sequences:

>Translated_427_residues
MNVLIIGSGAREHAMAWAAARNDAVQQVYVAPGNGGTALMGGKVQNVALKATALDELLEFAVLKAVDLTIAGSEQPLELG
IVDLFRSAQKKIIGPSQAAAQLETSKVFAKDFMSRYGIPTAGYHVFREFQTASLYLASLDESRYPQVIKASGLCAGKGVI
VASDGMEAFRATRAMFEDRVFGDAADEVVIEDFLEGEEASVFVLTDGEKYRMFYSAQDHKRIGEGDTGKNTGGMGAYAPA
PLVTSGVMKKIEEQIVVPALEGMAADGTPYTGFLYVGVMIDKGEPSVVEFNARMGDPETQVVLPLLKDDFIDALQASLQG
TLDLVPFEMLEQCATTVVIASKGYPDLYETGREITIDSELFDMRDVLLFHAGTEIEKGTLRTSGGRVFSVTALGKTLMDS
IELAYRAVDHISFEGACFRRDIGAKAL
>Mature_427_residues
MNVLIIGSGAREHAMAWAAARNDAVQQVYVAPGNGGTALMGGKVQNVALKATALDELLEFAVLKAVDLTIAGSEQPLELG
IVDLFRSAQKKIIGPSQAAAQLETSKVFAKDFMSRYGIPTAGYHVFREFQTASLYLASLDESRYPQVIKASGLCAGKGVI
VASDGMEAFRATRAMFEDRVFGDAADEVVIEDFLEGEEASVFVLTDGEKYRMFYSAQDHKRIGEGDTGKNTGGMGAYAPA
PLVTSGVMKKIEEQIVVPALEGMAADGTPYTGFLYVGVMIDKGEPSVVEFNARMGDPETQVVLPLLKDDFIDALQASLQG
TLDLVPFEMLEQCATTVVIASKGYPDLYETGREITIDSELFDMRDVLLFHAGTEIEKGTLRTSGGRVFSVTALGKTLMDS
IELAYRAVDHISFEGACFRRDIGAKAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=46.3700234192037, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=46.3700234192037, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=46.3700234192037, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=46.3700234192037, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=48.7179487179487, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567511, Length=432, Percent_Identity=40.0462962962963, Blast_Score=271, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=41.4027149321267, Blast_Score=316, Evalue=4e-87,
Organism=Drosophila melanogaster, GI62471619, Length=438, Percent_Identity=40.8675799086758, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24582400, Length=438, Percent_Identity=40.8675799086758, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45953; Mature: 45953

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGAREHAMAWAAARNDAVQQVYVAPGNGGTALMGGKVQNVALKATALDELLEF
CEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCEEEEHHHHHHHHHHH
AVLKAVDLTIAGSEQPLELGIVDLFRSAQKKIIGPSQAAAQLETSKVFAKDFMSRYGIPT
HHHHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AGYHVFREFQTASLYLASLDESRYPQVIKASGLCAGKGVIVASDGMEAFRATRAMFEDRV
HHHHHHHHHHHHEEEEEECCCCCCCCCEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
FGDAADEVVIEDFLEGEEASVFVLTDGEKYRMFYSAQDHKRIGEGDTGKNTGGMGAYAPA
CCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PLVTSGVMKKIEEQIVVPALEGMAADGTPYTGFLYVGVMIDKGEPSVVEFNARMGDPETQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCE
VVLPLLKDDFIDALQASLQGTLDLVPFEMLEQCATTVVIASKGYPDLYETGREITIDSEL
EEEHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEEEECCCCCHHHHCCCEEEECHHH
FDMRDVLLFHAGTEIEKGTLRTSGGRVFSVTALGKTLMDSIELAYRAVDHISFEGACFRR
HHHHHHHHEECCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DIGAKAL
CCCCCCC
>Mature Secondary Structure
MNVLIIGSGAREHAMAWAAARNDAVQQVYVAPGNGGTALMGGKVQNVALKATALDELLEF
CEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCEEEEHHHHHHHHHHH
AVLKAVDLTIAGSEQPLELGIVDLFRSAQKKIIGPSQAAAQLETSKVFAKDFMSRYGIPT
HHHHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AGYHVFREFQTASLYLASLDESRYPQVIKASGLCAGKGVIVASDGMEAFRATRAMFEDRV
HHHHHHHHHHHHEEEEEECCCCCCCCCEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
FGDAADEVVIEDFLEGEEASVFVLTDGEKYRMFYSAQDHKRIGEGDTGKNTGGMGAYAPA
CCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PLVTSGVMKKIEEQIVVPALEGMAADGTPYTGFLYVGVMIDKGEPSVVEFNARMGDPETQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCE
VVLPLLKDDFIDALQASLQGTLDLVPFEMLEQCATTVVIASKGYPDLYETGREITIDSEL
EEEHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEEEECCCCCHHHHCCCEEEECHHH
FDMRDVLLFHAGTEIEKGTLRTSGGRVFSVTALGKTLMDSIELAYRAVDHISFEGACFRR
HHHHHHHHEECCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DIGAKAL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]