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The map label for this gene is hisH [H]

Identifier: 119356328

GI number: 119356328

Start: 538882

End: 539487

Strand: Direct

Name: hisH [H]

Synonym: Cpha266_0491

Alternate gene names: 119356328

Gene position: 538882-539487 (Clockwise)

Preceding gene: 119356320

Following gene: 119356329

Centisome position: 17.2

GC content: 44.06

Gene sequence:

>606_bases
ATGGTTTTTATTGCTGACTATGGGGCAGGAAATCTCCGTTCTGTTCAGAAAGCTTTTGATTATCTCGGAGTAAACTCTGT
GGTCAGCAGTGATCCCGGGAAACTTGTCGGGTGTAAAAAAGTACTTTTGCCTGGCGTTGGAGCGTTTGGCCAGGCAATTG
ATTCATTGAATAGGTCGGGATTTACCGATGCCGTTCGTGAACATGTTGACAAAGGCGGTCACTTGCTCGGTATTTGCCTT
GGCATGCAGCTTTTGCTTAGCGAAAGCGAAGAGATGGGTTCTCATTCCGGCATGAATCTTGTACCGGGAAAAGTGAGGCA
TTTCAGAAGCGCAACCGATAAAATTCCACAGATAGGATGGAATTCCATTCAGATGCAGAAAGAAAGTCTTCTGTTCAGAG
GTATGCCGGATGGTTCTTTTTTTTATTTTGTACACTCCTATTATTGTGAACCTGAAGATAATGATGCCGTGGCGGCGGTA
ACCTGGTTTGCGGGAAATAATTTTTGTTCCGCCATTGAAAAAAATGGTATTTTTGCAGTCCAGTTTCATCCTGAAAAAAG
TTCCGAGGCCGGTTTACAGGTGCTGAAGAATTTTGCAGGGTGTTAA

Upstream 100 bases:

>100_bases
TATAATAAGCATTAATCATCCAATTTAAAACCGGATTTTTTTTATACAGGTATCGCGGGCACCCGGTTTTGGGCGCCCGT
AACCGTTAACTCTTTTTTTT

Downstream 100 bases:

>100_bases
GAATCGTTTGTTTTGTTTTTTTATGCTGATAATTCCTGCTATAGACATTAAGGACGGAAGATGTGTGCGGTTGACTCGTG
GTGATTTCAACCAGCAGAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MVFIADYGAGNLRSVQKAFDYLGVNSVVSSDPGKLVGCKKVLLPGVGAFGQAIDSLNRSGFTDAVREHVDKGGHLLGICL
GMQLLLSESEEMGSHSGMNLVPGKVRHFRSATDKIPQIGWNSIQMQKESLLFRGMPDGSFFYFVHSYYCEPEDNDAVAAV
TWFAGNNFCSAIEKNGIFAVQFHPEKSSEAGLQVLKNFAGC

Sequences:

>Translated_201_residues
MVFIADYGAGNLRSVQKAFDYLGVNSVVSSDPGKLVGCKKVLLPGVGAFGQAIDSLNRSGFTDAVREHVDKGGHLLGICL
GMQLLLSESEEMGSHSGMNLVPGKVRHFRSATDKIPQIGWNSIQMQKESLLFRGMPDGSFFYFVHSYYCEPEDNDAVAAV
TWFAGNNFCSAIEKNGIFAVQFHPEKSSEAGLQVLKNFAGC
>Mature_201_residues
MVFIADYGAGNLRSVQKAFDYLGVNSVVSSDPGKLVGCKKVLLPGVGAFGQAIDSLNRSGFTDAVREHVDKGGHLLGICL
GMQLLLSESEEMGSHSGMNLVPGKVRHFRSATDKIPQIGWNSIQMQKESLLFRGMPDGSFFYFVHSYYCEPEDNDAVAAV
TWFAGNNFCSAIEKNGIFAVQFHPEKSSEAGLQVLKNFAGC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=37.0558375634518, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=36.0576923076923, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21791; Mature: 21791

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFIADYGAGNLRSVQKAFDYLGVNSVVSSDPGKLVGCKKVLLPGVGAFGQAIDSLNRSG
CEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCEECHHHHHHCCCCHHHHHHHHHCCCC
FTDAVREHVDKGGHLLGICLGMQLLLSESEEMGSHSGMNLVPGKVRHFRSATDKIPQIGW
CHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
NSIQMQKESLLFRGMPDGSFFYFVHSYYCEPEDNDAVAAVTWFAGNNFCSAIEKNGIFAV
CHHHHHHHHHHEECCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHCCCEEEE
QFHPEKSSEAGLQVLKNFAGC
EECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVFIADYGAGNLRSVQKAFDYLGVNSVVSSDPGKLVGCKKVLLPGVGAFGQAIDSLNRSG
CEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCEECHHHHHHCCCCHHHHHHHHHCCCC
FTDAVREHVDKGGHLLGICLGMQLLLSESEEMGSHSGMNLVPGKVRHFRSATDKIPQIGW
CHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
NSIQMQKESLLFRGMPDGSFFYFVHSYYCEPEDNDAVAAVTWFAGNNFCSAIEKNGIFAV
CHHHHHHHHHHEECCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHCCCEEEE
QFHPEKSSEAGLQVLKNFAGC
EECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA