Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 119356312

GI number: 119356312

Start: 523801

End: 525831

Strand: Direct

Name: ligA

Synonym: Cpha266_0475

Alternate gene names: 119356312

Gene position: 523801-525831 (Clockwise)

Preceding gene: 119356311

Following gene: 119356313

Centisome position: 16.71

GC content: 52.44

Gene sequence:

>2031_bases
ATGAGCGATGCCGTCAATGTCCGCAAGGCTATAGAAGAACTACGCAGGGAGATCAACAGGCACAATACGCTCTACTATGT
TGATGCCCGTCCTGAAATTTCCGATTACGAGTTTGATCGGTTGATGGAACGCCTCATCGAACTCGAAAAGGCTTTTCCGG
ATTTTGTCACACCGGACAGCCCTTCTCATCGGGTTGGTGGTGCAATTACCAGAGAGTTTCCTTCGGTACGGCACAGGGAG
CCGATGCTGAGCCTTTCCAATACCTACTCGCTTGCAGAGGTCGAGGATTTTTACAGTCGGGTGAAAAAACTTCTGCTGGT
GGAAGGGGTGCGCGAGCAGGATATGGCAGCCGAACTGAAGTTTGACGGGGTGGCCATAAGTCTGCTGTATCGCGATGGTC
TTCTCGTTCAGGGTGCGACAAGAGGTGACGGTACCGAGGGGGACGATATTACTACAAATCTGAAAACCTTGTCGAGTGTG
CCGCTCAGGATTCCTCTGACAAGCCTTCCGGTTATTGAGGGCATAGAGCGCGATATCGAGATCCGCGGAGAGGTGTTCAT
GCAGAAGGATGATTTTGCCCGTCTTAACGAGGCTCGTCCTGAAGAAGACCGGTTTGCCAATCCTCGTAATGCAACTGCCG
GAACGCTTAAATTGCAGGATTCCGGAGAGGTTGCCCGTCGACGTTTGAATTTTGTGGCTTATTATCTGCGGCTTTCGAGC
CAGGACTCTTTTCTGCTTACCCAGTATGACCGGCTTGAACTGCTCGGGCAGCTCGGTTTCTTTACCGGGAAGCACTACAG
GTTATGTCGCGATATGAAGGAAATCGGTGATTTTATCGGCTATTGGGCCGTTGAGAGGTGGAACCTTCCTTATGAAACGG
ACGGAGTTGTCCTGAAACTCAACGATGTCGCACTATGGCCGAGAATTGGCGCAACCGCAAAAAGTCCTCGCTGGGCTATT
GCCTACAAGTATCCCGCACAGCAGGCGAAAACAGTTCTGCAAGGGGTGGCATTTCAGGTCGGCAGACTCGGAACGGTGAC
TCCTGTTGCTGAACTTGAGCCTGTTCGGCTTGCCGGTTCAACGGTGTCGCGTTCAACCCTGCATAATTTTGACGAGATAG
AGCGGCTTGGTCTCATGCTTCATGATCGGGTGATTATTGAAAAATCAGGGGAGGTTATCCCCAAAGTGATTCGGGTTGTT
CTCGATGAACGACCCGAAAATGCAGAGCCTGTCGGCGTGCCTTCGGTTTGTCCCGAGTGCGGCGCTTCGCTCGAAAAACC
CGAAAACGAGGTCAGTTACTACTGTCCCGATCAAGATTCGTGCCCGGCACAGATCAGGGGACGCTTGCTCCATTTTGCTT
CGCGAAATGCCCTTGACATTCAGTCGCTTGGTGAATCGCTGGTCGCTCAGCTTGTTCAAAAGGGTCTTGTGAGGGATGCC
GGAGATCTTTATCTTCTTCAGCAACCGCAGCTTGAGGCTCTTGAGAGGATGGGACGAAAATCGGCGCACAATCTCCTTCG
CGCACTTCAAGAGAGTCGGGAGAAGAGTTATGAACGCTTGCTCTATGCGCTTGGGATTCGTCATGTCGGTCAGGCTACGG
CCCGTGAACTTGCCAGGGCCTATGAGACGGTTGAGGCCTTGCAGAATGCTTCGGAGGAGGAGCTCGCAACGGTGCCTGAT
GTCGGGCCGGTTGTTGCCCATAGCGTCAAGGACTATTTTTCAAAATCGTCTACACAGATGCTGCTCCGGAAACTCAGGGA
TGCCGGCTTTCCTTTACGTTTCACCGGGCCTCAAAAGCTGATCAACAGGAATTTCGAGGGTGTGAATGTTCTGTTTACGG
GAGCGCTCGAACGGTATGGACGGCAACAGGCATCGGAACTTGTTCAGCAACGGGGAGGGAGGGTTGTTGGTTCGGTCAGC
AGAAGCACCGGAGTTGTTGTTGCCGGTAGTGAACCCGGCAGCAAGCTTGACAAAGCGAGAAAGCTCGGCGTAAAGGTGAT
CAGCGAGGATGAATTTAATGCAATGTTGTAA

Upstream 100 bases:

>100_bases
AACCTGGGATAAGCTTGGCGTTGCCGGTTCGACGGGTTTTTTGAAAAATCTCGTCGGCTGGGTCTTCTCTTCAGCCAGAT
AACTCTTCATATCATTCGTC

Downstream 100 bases:

>100_bases
CGTATAGTATTATGGACGCTTATTATCGAGTGGCGATGTTTGGTTCTGCAAGGATACAGGAGGGCGATCAGGATTACCGG
GATGTTTTTCATATAGCGAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 676; Mature: 675

Protein sequence:

>676_residues
MSDAVNVRKAIEELRREINRHNTLYYVDARPEISDYEFDRLMERLIELEKAFPDFVTPDSPSHRVGGAITREFPSVRHRE
PMLSLSNTYSLAEVEDFYSRVKKLLLVEGVREQDMAAELKFDGVAISLLYRDGLLVQGATRGDGTEGDDITTNLKTLSSV
PLRIPLTSLPVIEGIERDIEIRGEVFMQKDDFARLNEARPEEDRFANPRNATAGTLKLQDSGEVARRRLNFVAYYLRLSS
QDSFLLTQYDRLELLGQLGFFTGKHYRLCRDMKEIGDFIGYWAVERWNLPYETDGVVLKLNDVALWPRIGATAKSPRWAI
AYKYPAQQAKTVLQGVAFQVGRLGTVTPVAELEPVRLAGSTVSRSTLHNFDEIERLGLMLHDRVIIEKSGEVIPKVIRVV
LDERPENAEPVGVPSVCPECGASLEKPENEVSYYCPDQDSCPAQIRGRLLHFASRNALDIQSLGESLVAQLVQKGLVRDA
GDLYLLQQPQLEALERMGRKSAHNLLRALQESREKSYERLLYALGIRHVGQATARELARAYETVEALQNASEEELATVPD
VGPVVAHSVKDYFSKSSTQMLLRKLRDAGFPLRFTGPQKLINRNFEGVNVLFTGALERYGRQQASELVQQRGGRVVGSVS
RSTGVVVAGSEPGSKLDKARKLGVKVISEDEFNAML

Sequences:

>Translated_676_residues
MSDAVNVRKAIEELRREINRHNTLYYVDARPEISDYEFDRLMERLIELEKAFPDFVTPDSPSHRVGGAITREFPSVRHRE
PMLSLSNTYSLAEVEDFYSRVKKLLLVEGVREQDMAAELKFDGVAISLLYRDGLLVQGATRGDGTEGDDITTNLKTLSSV
PLRIPLTSLPVIEGIERDIEIRGEVFMQKDDFARLNEARPEEDRFANPRNATAGTLKLQDSGEVARRRLNFVAYYLRLSS
QDSFLLTQYDRLELLGQLGFFTGKHYRLCRDMKEIGDFIGYWAVERWNLPYETDGVVLKLNDVALWPRIGATAKSPRWAI
AYKYPAQQAKTVLQGVAFQVGRLGTVTPVAELEPVRLAGSTVSRSTLHNFDEIERLGLMLHDRVIIEKSGEVIPKVIRVV
LDERPENAEPVGVPSVCPECGASLEKPENEVSYYCPDQDSCPAQIRGRLLHFASRNALDIQSLGESLVAQLVQKGLVRDA
GDLYLLQQPQLEALERMGRKSAHNLLRALQESREKSYERLLYALGIRHVGQATARELARAYETVEALQNASEEELATVPD
VGPVVAHSVKDYFSKSSTQMLLRKLRDAGFPLRFTGPQKLINRNFEGVNVLFTGALERYGRQQASELVQQRGGRVVGSVS
RSTGVVVAGSEPGSKLDKARKLGVKVISEDEFNAML
>Mature_675_residues
SDAVNVRKAIEELRREINRHNTLYYVDARPEISDYEFDRLMERLIELEKAFPDFVTPDSPSHRVGGAITREFPSVRHREP
MLSLSNTYSLAEVEDFYSRVKKLLLVEGVREQDMAAELKFDGVAISLLYRDGLLVQGATRGDGTEGDDITTNLKTLSSVP
LRIPLTSLPVIEGIERDIEIRGEVFMQKDDFARLNEARPEEDRFANPRNATAGTLKLQDSGEVARRRLNFVAYYLRLSSQ
DSFLLTQYDRLELLGQLGFFTGKHYRLCRDMKEIGDFIGYWAVERWNLPYETDGVVLKLNDVALWPRIGATAKSPRWAIA
YKYPAQQAKTVLQGVAFQVGRLGTVTPVAELEPVRLAGSTVSRSTLHNFDEIERLGLMLHDRVIIEKSGEVIPKVIRVVL
DERPENAEPVGVPSVCPECGASLEKPENEVSYYCPDQDSCPAQIRGRLLHFASRNALDIQSLGESLVAQLVQKGLVRDAG
DLYLLQQPQLEALERMGRKSAHNLLRALQESREKSYERLLYALGIRHVGQATARELARAYETVEALQNASEEELATVPDV
GPVVAHSVKDYFSKSSTQMLLRKLRDAGFPLRFTGPQKLINRNFEGVNVLFTGALERYGRQQASELVQQRGGRVVGSVSR
STGVVVAGSEPGSKLDKARKLGVKVISEDEFNAML

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=675, Percent_Identity=42.0740740740741, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI87082305, Length=536, Percent_Identity=23.8805970149254, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHLPD (A1BDQ5)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910956.1
- ProteinModelPortal:   A1BDQ5
- STRING:   A1BDQ5
- GeneID:   4568519
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0475
- NMPDR:   fig|290317.7.peg.492
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75837; Mature: 75706

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 120-120 BINDING 118-118 BINDING 141-141 BINDING 184-184 BINDING 299-299 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAVNVRKAIEELRREINRHNTLYYVDARPEISDYEFDRLMERLIELEKAFPDFVTPDS
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PSHRVGGAITREFPSVRHREPMLSLSNTYSLAEVEDFYSRVKKLLLVEGVREQDMAAELK
CCCCCCCHHHHHCCCCHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHEE
FDGVAISLLYRDGLLVQGATRGDGTEGDDITTNLKTLSSVPLRIPLTSLPVIEGIERDIE
ECCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCHHHHHCCCCCE
IRGEVFMQKDDFARLNEARPEEDRFANPRNATAGTLKLQDSGEVARRRLNFVAYYLRLSS
ECCCEEECCCHHHHHHCCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEECC
QDSFLLTQYDRLELLGQLGFFTGKHYRLCRDMKEIGDFIGYWAVERWNLPYETDGVVLKL
CCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEE
NDVALWPRIGATAKSPRWAIAYKYPAQQAKTVLQGVAFQVGRLGTVTPVAELEPVRLAGS
CCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEEECCC
TVSRSTLHNFDEIERLGLMLHDRVIIEKSGEVIPKVIRVVLDERPENAEPVGVPSVCPEC
CHHHHHHHCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
GASLEKPENEVSYYCPDQDSCPAQIRGRLLHFASRNALDIQSLGESLVAQLVQKGLVRDA
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
GDLYLLQQPQLEALERMGRKSAHNLLRALQESREKSYERLLYALGIRHVGQATARELARA
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETVEALQNASEEELATVPDVGPVVAHSVKDYFSKSSTQMLLRKLRDAGFPLRFTGPQKL
HHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCHHHH
INRNFEGVNVLFTGALERYGRQQASELVQQRGGRVVGSVSRSTGVVVAGSEPGSKLDKAR
HCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHH
KLGVKVISEDEFNAML
HHCCEEECCCCCCCCC
>Mature Secondary Structure 
SDAVNVRKAIEELRREINRHNTLYYVDARPEISDYEFDRLMERLIELEKAFPDFVTPDS
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PSHRVGGAITREFPSVRHREPMLSLSNTYSLAEVEDFYSRVKKLLLVEGVREQDMAAELK
CCCCCCCHHHHHCCCCHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHEE
FDGVAISLLYRDGLLVQGATRGDGTEGDDITTNLKTLSSVPLRIPLTSLPVIEGIERDIE
ECCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCHHHHHCCCCCE
IRGEVFMQKDDFARLNEARPEEDRFANPRNATAGTLKLQDSGEVARRRLNFVAYYLRLSS
ECCCEEECCCHHHHHHCCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEECC
QDSFLLTQYDRLELLGQLGFFTGKHYRLCRDMKEIGDFIGYWAVERWNLPYETDGVVLKL
CCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEE
NDVALWPRIGATAKSPRWAIAYKYPAQQAKTVLQGVAFQVGRLGTVTPVAELEPVRLAGS
CCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEEECCC
TVSRSTLHNFDEIERLGLMLHDRVIIEKSGEVIPKVIRVVLDERPENAEPVGVPSVCPEC
CHHHHHHHCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
GASLEKPENEVSYYCPDQDSCPAQIRGRLLHFASRNALDIQSLGESLVAQLVQKGLVRDA
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
GDLYLLQQPQLEALERMGRKSAHNLLRALQESREKSYERLLYALGIRHVGQATARELARA
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETVEALQNASEEELATVPDVGPVVAHSVKDYFSKSSTQMLLRKLRDAGFPLRFTGPQKL
HHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCHHHH
INRNFEGVNVLFTGALERYGRQQASELVQQRGGRVVGSVSRSTGVVVAGSEPGSKLDKAR
HCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHH
KLGVKVISEDEFNAML
HHCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA