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The map label for this gene is algU [H]

Identifier: 119356247

GI number: 119356247

Start: 451326

End: 451934

Strand: Reverse

Name: algU [H]

Synonym: Cpha266_0408

Alternate gene names: 119356247

Gene position: 451934-451326 (Counterclockwise)

Preceding gene: 119356248

Following gene: 119356246

Centisome position: 14.42

GC content: 45.98

Gene sequence:

>609_bases
ATGGAAAAAAGAGGAGAGGCCGCAGTAACGATCCCTGCAACCCACGAAGCATTGCTGGATAGTCGGGCAACATCGAAAGA
CGATCAGTTCAGAAAGCTGGTTCTTGAGCATCAGGAGATGGTGCTCAATACCTGTTTCCGTTTTGTATTCAACCGTGAAG
ATGCCGAAGACCTTGCTCAGGATGTTTTTGTGGAAGTATACCGCTCGCTTGATCAGTTCAGGGAAGAATCAAAACTTTCT
ACATGGATCTACCGTATTGCTGTCACCAAATCGCTTGACTATCTGAGAAGACTGAAACGAAAAAAACGGTTCAGCTCTCT
CAAAAGAGTTATCGGGATTGAAGACCCTTCGGAAGATCTGCCGGATACAGCGGGAAGCAATCCTCTTCAGTCGCTTGCCG
GAAAAGAAAACGCAAATCTTCTTCTGGACGCACTGAATGCGCTACCTGAAAACCAGAAAACGGCATTTCTGCTCAGCAAA
CAGGATGGCTACTCCAACCAGGAAATAGCCGATATTCTCCAGAGTTCCGTTTCAGCAGTCGAATCACTCATCCACAGGGC
AAAAAAAAACCTGCACGACAAACTCTATAAACATTTCAAACAGACCTGA

Upstream 100 bases:

>100_bases
TTTGAACTATTGAACAAAGGAATCGAAAAAAAACGGCGTCCAACCCTTCGAGTATCTATTGCACGAAAAGCATAACCGGC
AAAACAGATGATGCCTGCAC

Downstream 100 bases:

>100_bases
AACACTGTTGAAAAGGAGAACCGAAGGATTTTTGCCAGTCTGACGTCAGATATATCATGAAACAGGAAATAAAGATGAAA
CATAACATCGACACAAAAGA

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MEKRGEAAVTIPATHEALLDSRATSKDDQFRKLVLEHQEMVLNTCFRFVFNREDAEDLAQDVFVEVYRSLDQFREESKLS
TWIYRIAVTKSLDYLRRLKRKKRFSSLKRVIGIEDPSEDLPDTAGSNPLQSLAGKENANLLLDALNALPENQKTAFLLSK
QDGYSNQEIADILQSSVSAVESLIHRAKKNLHDKLYKHFKQT

Sequences:

>Translated_202_residues
MEKRGEAAVTIPATHEALLDSRATSKDDQFRKLVLEHQEMVLNTCFRFVFNREDAEDLAQDVFVEVYRSLDQFREESKLS
TWIYRIAVTKSLDYLRRLKRKKRFSSLKRVIGIEDPSEDLPDTAGSNPLQSLAGKENANLLLDALNALPENQKTAFLLSK
QDGYSNQEIADILQSSVSAVESLIHRAKKNLHDKLYKHFKQT
>Mature_202_residues
MEKRGEAAVTIPATHEALLDSRATSKDDQFRKLVLEHQEMVLNTCFRFVFNREDAEDLAQDVFVEVYRSLDQFREESKLS
TWIYRIAVTKSLDYLRRLKRKKRFSSLKRVIGIEDPSEDLPDTAGSNPLQSLAGKENANLLLDALNALPENQKTAFLLSK
QDGYSNQEIADILQSSVSAVESLIHRAKKNLHDKLYKHFKQT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=177, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23169; Mature: 23169

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRGEAAVTIPATHEALLDSRATSKDDQFRKLVLEHQEMVLNTCFRFVFNREDAEDLAQ
CCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DVFVEVYRSLDQFREESKLSTWIYRIAVTKSLDYLRRLKRKKRFSSLKRVIGIEDPSEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDTAGSNPLQSLAGKENANLLLDALNALPENQKTAFLLSKQDGYSNQEIADILQSSVSAV
CCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
ESLIHRAKKNLHDKLYKHFKQT
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKRGEAAVTIPATHEALLDSRATSKDDQFRKLVLEHQEMVLNTCFRFVFNREDAEDLAQ
CCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DVFVEVYRSLDQFREESKLSTWIYRIAVTKSLDYLRRLKRKKRFSSLKRVIGIEDPSEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDTAGSNPLQSLAGKENANLLLDALNALPENQKTAFLLSKQDGYSNQEIADILQSSVSAV
CCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
ESLIHRAKKNLHDKLYKHFKQT
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]