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The map label for this gene is trpB2 [H]

Identifier: 119356174

GI number: 119356174

Start: 365166

End: 366539

Strand: Reverse

Name: trpB2 [H]

Synonym: Cpha266_0335

Alternate gene names: 119356174

Gene position: 366539-365166 (Counterclockwise)

Preceding gene: 119356175

Following gene: 119356172

Centisome position: 11.7

GC content: 53.06

Gene sequence:

>1374_bases
ATGGGTACAGATCTCACCAAGATCATTCTTGACGAACATGAAATGCCCCGTCAGTGGTACAACATTCAGGCTGACCTTCC
TGTACCATTACCCCCGCCAGTGGGCATGGACGGCACCCCGATATCTCCCGACGATCTGGCACAGGTGTTTCCGATGAACC
TGATTGAGCAGGAGATGAGCACCGAGCGATGGATAGACATCCCTGAGGAGGTGCTCTCGATCCTGAAACTCTGGCGCCCT
TCGCCGCTCTATCGTGCAAAGCGTCTCGAAAAAGCCCTCGGAACTCCGGCAAAAATCTTTTATAAAAACGAAGGTGTTTC
GCCTGCGGGAAGTCACAAACCAAACACGGCTGTTCCCCAGGCATGGTACAACAAGCAGTTCGGTATAAAATATCTTACCA
CCGAAACCGGCGCAGGGCAGTGGGGCAGCGCTCTTGCGATGAGCTGCAAGCTTATCGGTATTGAGTGCAAGGTCTTTATG
GTTCGCATCAGTTTCGATCAGAAACCGTTTCGAAAAATCATGATGAAAACCTGGGGCGCAGAGTGCATTGCCAGCCCCAG
CCGCGAAACGGCAATCGGACGACGCATTCTGGAGGAGATGCCTGATACACCGGGAAGCCTCGGTATTGCCATAAGCGAAG
CAATCGAACAGGCTGTAGAGCGTGAGGATACCCGATATGCTCTCGGCAGCGTGCTGAACCATGTGATGCTTCACCAGAGC
ATCATCGGTCTTGAAGCTCAAAAACAGTTTGAAAAAATCGGACTCTACCCCGACGTCGTTATCGGGTGCGCAGGAGGCGG
ATCGAATTTTGCCGGCATCAGTTTTCCTTTCATCTGCGACAAGATTCATGGAAAAGATATTCAGATCATTGCTACCGAGC
CCGAAGCCTGCCCGACGCTGACCAAAGGCCCCTATATTTATGATTCCGGAGATGTGGCATTAATGACTCCGCTGCTCGCC
ATGCACAGTCTCGGTCACGGCTTCATTCCTCCTGCGATACATGCCGGAGGGCTCCGCTATCACGGCATGGCGCCACTGGT
CAGCCATGTCAAACAGCTCGGCCTGATTGAGGCAACCGCACTGCCGCAGACAGAGTGCTATGAAGCCGCTCTGCTGTTTG
CGCACACCGAAGGCTTTATCCCTGCACCGGAAACCTCTCACGCTATTGCGCAAACCATCCGTGAAGCTAAAAAAGCAAAA
GAGGAAGGAAAAGAACGGGTTATTCTCATGAACTGGTCCGGCCACGGACTCATGGATCTGCAAGGCTACGATGCCTTTCT
TTCCGGCAAACTCAGCGATTACGCCCTGCCGGATGAGCTTTTGCAACAATCACTTGCCGCAGTCAGGAATCATCCCAAAC
CGCCAGGCGCATAA

Upstream 100 bases:

>100_bases
TTTTGAAGATCATTACCCAATGTCACCGATATTGAGTAAGTTTGCAACCAGTTACGCTTTTCCGGCAGCTTTTCCCGAAA
ACGCACAAACCATTAAAACC

Downstream 100 bases:

>100_bases
GAAACAAAAACCCCCGGATCAACCGGGGGTTTTTGTTTCTTAACTGTTAGGATCGGGAACCTCTTTATCGAGTTTTTCAT
CCCGTTTTCGTTTTTTCGTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MGTDLTKIILDEHEMPRQWYNIQADLPVPLPPPVGMDGTPISPDDLAQVFPMNLIEQEMSTERWIDIPEEVLSILKLWRP
SPLYRAKRLEKALGTPAKIFYKNEGVSPAGSHKPNTAVPQAWYNKQFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDQKPFRKIMMKTWGAECIASPSRETAIGRRILEEMPDTPGSLGIAISEAIEQAVEREDTRYALGSVLNHVMLHQS
IIGLEAQKQFEKIGLYPDVVIGCAGGGSNFAGISFPFICDKIHGKDIQIIATEPEACPTLTKGPYIYDSGDVALMTPLLA
MHSLGHGFIPPAIHAGGLRYHGMAPLVSHVKQLGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKKAK
EEGKERVILMNWSGHGLMDLQGYDAFLSGKLSDYALPDELLQQSLAAVRNHPKPPGA

Sequences:

>Translated_457_residues
MGTDLTKIILDEHEMPRQWYNIQADLPVPLPPPVGMDGTPISPDDLAQVFPMNLIEQEMSTERWIDIPEEVLSILKLWRP
SPLYRAKRLEKALGTPAKIFYKNEGVSPAGSHKPNTAVPQAWYNKQFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDQKPFRKIMMKTWGAECIASPSRETAIGRRILEEMPDTPGSLGIAISEAIEQAVEREDTRYALGSVLNHVMLHQS
IIGLEAQKQFEKIGLYPDVVIGCAGGGSNFAGISFPFICDKIHGKDIQIIATEPEACPTLTKGPYIYDSGDVALMTPLLA
MHSLGHGFIPPAIHAGGLRYHGMAPLVSHVKQLGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKKAK
EEGKERVILMNWSGHGLMDLQGYDAFLSGKLSDYALPDELLQQSLAAVRNHPKPPGA
>Mature_456_residues
GTDLTKIILDEHEMPRQWYNIQADLPVPLPPPVGMDGTPISPDDLAQVFPMNLIEQEMSTERWIDIPEEVLSILKLWRPS
PLYRAKRLEKALGTPAKIFYKNEGVSPAGSHKPNTAVPQAWYNKQFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMV
RISFDQKPFRKIMMKTWGAECIASPSRETAIGRRILEEMPDTPGSLGIAISEAIEQAVEREDTRYALGSVLNHVMLHQSI
IGLEAQKQFEKIGLYPDVVIGCAGGGSNFAGISFPFICDKIHGKDIQIIATEPEACPTLTKGPYIYDSGDVALMTPLLAM
HSLGHGFIPPAIHAGGLRYHGMAPLVSHVKQLGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKKAKE
EGKERVILMNWSGHGLMDLQGYDAFLSGKLSDYALPDELLQQSLAAVRNHPKPPGA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=380, Percent_Identity=27.3684210526316, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321412, Length=364, Percent_Identity=30.7692307692308, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 50115; Mature: 49983

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTDLTKIILDEHEMPRQWYNIQADLPVPLPPPVGMDGTPISPDDLAQVFPMNLIEQEMS
CCCCHHHHHHCCCCCCHHHCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TERWIDIPEEVLSILKLWRPSPLYRAKRLEKALGTPAKIFYKNEGVSPAGSHKPNTAVPQ
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCH
AWYNKQFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDQKPFRKIMMKTWGA
HHHCCCCCEEEEEECCCCCCCCHHHHHEEEEEEEEEEEEEEEEECCCCHHHHHHHHHCCH
ECIASPSRETAIGRRILEEMPDTPGSLGIAISEAIEQAVEREDTRYALGSVLNHVMLHQS
HHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGLEAQKQFEKIGLYPDVVIGCAGGGSNFAGISFPFICDKIHGKDIQIIATEPEACPTL
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCC
TKGPYIYDSGDVALMTPLLAMHSLGHGFIPPAIHAGGLRYHGMAPLVSHVKQLGLIEATA
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHCCC
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKKAKEEGKERVILMNWSGHGLMDL
CCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
QGYDAFLSGKLSDYALPDELLQQSLAAVRNHPKPPGA
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GTDLTKIILDEHEMPRQWYNIQADLPVPLPPPVGMDGTPISPDDLAQVFPMNLIEQEMS
CCCHHHHHHCCCCCCHHHCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TERWIDIPEEVLSILKLWRPSPLYRAKRLEKALGTPAKIFYKNEGVSPAGSHKPNTAVPQ
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCH
AWYNKQFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDQKPFRKIMMKTWGA
HHHCCCCCEEEEEECCCCCCCCHHHHHEEEEEEEEEEEEEEEEECCCCHHHHHHHHHCCH
ECIASPSRETAIGRRILEEMPDTPGSLGIAISEAIEQAVEREDTRYALGSVLNHVMLHQS
HHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGLEAQKQFEKIGLYPDVVIGCAGGGSNFAGISFPFICDKIHGKDIQIIATEPEACPTL
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCC
TKGPYIYDSGDVALMTPLLAMHSLGHGFIPPAIHAGGLRYHGMAPLVSHVKQLGLIEATA
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHCCC
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKKAKEEGKERVILMNWSGHGLMDL
CCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
QGYDAFLSGKLSDYALPDELLQQSLAAVRNHPKPPGA
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]