Click here to switch to the map view.

The map label for this gene is eno

Identifier: 119356070

GI number: 119356070

Start: 248120

End: 249433

Strand: Direct

Name: eno

Synonym: Cpha266_0223

Alternate gene names: 119356070

Gene position: 248120-249433 (Clockwise)

Preceding gene: 119356068

Following gene: 119356071

Centisome position: 7.92

GC content: 46.8

Gene sequence:

>1314_bases
ATGCCGATAATCAGGAAAATTCTTGCCAGACAGATACTGGATTCACGTGGAAATCCTACGGTTGAAGTGGATGTTTACAC
TGAAAATTCATTTGGGAGGGCTGCTGTACCGAGCGGAGCTTCAACAGGTGTTCATGAAGCGGTAGAACTCAGAGATGGTG
ATAGTGCCGTCTATCTTGGTAAGGGTGTTCTTAAGGCTGTCGATCATGTTAATACAGTTATCAACGACAGATTGAAAGGA
ATGTTTGTTACCGATCAGGAAGAGATTGATATGGCAATGCTTGCTTTGGATGGAACGCCGAACAAATCAAGACTTGGAGC
GAATGCTCTCCTGGGCGTTTCAATGGCATGTGCCAAAGCCGGGGCGGAATACAGCGGTCTTCCTCTTTACCGCTATATCG
GCGGAACGATGGCAAACACGCTTCCTGTTCCGATGATGAATGTTTTGAATGGCGGAGCTCATGCCGATAATACGGTTGAT
TTTCAGGAGTTTATGATTATGCCTGTTGGTTTCAGCAGTTATTCTGATGCGCTGCGGTCAGGTGCCGAAATATTCCATGC
GTTAAAATCTCTTTTGAAAAGCAAAGGATTGAGTACTGCTGTTGGCGATGAAGGTGGTTTTGCGCCGAATCTGAAATCGA
ACGAAGAGGCAATTGAACTCGTCATTGAGGCGGTAGGAAAAGCCGGATATAAAATCGGCTCTTCCACGGCAAACGGGGGT
CTTGGGGATGCACAGGTGATGATCGCTCTCGATCCCGCGAGCTCTGAATTTTATGATGCAGCGAAGCAAAAGTATGTTTT
TAAAAAATCGTCAAAACAAGAGCTCTCTTCGCTTGAAATGGCCGAATACTGGGAGAAATGGGCCAGCGATTATCCGATTA
TATCCATTGAAGACGGTATGGCTGAAGATGACTGGGATGGCTGGAAGATATTGACAGATAAAATCGGTTCCAGAGTGCAG
CTCGTTGGTGATGATCTTTTTGTTACCAATACAAGCCGACTTGCTCTTGGTATTGAGCGCGGCGTAGGAAATTCGATTTT
GGTTAAAGTTAATCAGATCGGAACGCTTACAGAGACGTTGCAGGCTATTGATCTGGCCAGGCGAAATGGTTATACCGCGG
TGATAAGTCATCGAAGCGGAGAAACCGAAGACAGCACGATAGCCCAGATTGCCGTGGCGACCAATGCCGGACAGATTAAA
ACCGGGAGCTTGTCTCGTTCAGACCGTATGGCAAAATACAATGAGCTGCTGCGTATTGAAGAGGAACTTGGCCTGCAGGC
GAAGTATCCGGCTCTTTCCGCTTTTCGCGTTTAA

Upstream 100 bases:

>100_bases
GTAAGGAAAAAAGATAAATTGGAAGTCTGAAAGGGAGCTTTTTAACCCACATTCCTTCTCATCTGTGTTTAAAATTCAAT
AATCATAAATATAGCGCGTT

Downstream 100 bases:

>100_bases
TTTTAATAGAGGATCTTTTCGGTAGCAAAAGATCCTCGTTTTTTTCTTGTGAGGGCACCTCTATGTATTTGTTGCGCGAA
TCCCGACTTGTCGGGAGCAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPIIRKILARQILDSRGNPTVEVDVYTENSFGRAAVPSGASTGVHEAVELRDGDSAVYLGKGVLKAVDHVNTVINDRLKG
MFVTDQEEIDMAMLALDGTPNKSRLGANALLGVSMACAKAGAEYSGLPLYRYIGGTMANTLPVPMMNVLNGGAHADNTVD
FQEFMIMPVGFSSYSDALRSGAEIFHALKSLLKSKGLSTAVGDEGGFAPNLKSNEEAIELVIEAVGKAGYKIGSSTANGG
LGDAQVMIALDPASSEFYDAAKQKYVFKKSSKQELSSLEMAEYWEKWASDYPIISIEDGMAEDDWDGWKILTDKIGSRVQ
LVGDDLFVTNTSRLALGIERGVGNSILVKVNQIGTLTETLQAIDLARRNGYTAVISHRSGETEDSTIAQIAVATNAGQIK
TGSLSRSDRMAKYNELLRIEEELGLQAKYPALSAFRV

Sequences:

>Translated_437_residues
MPIIRKILARQILDSRGNPTVEVDVYTENSFGRAAVPSGASTGVHEAVELRDGDSAVYLGKGVLKAVDHVNTVINDRLKG
MFVTDQEEIDMAMLALDGTPNKSRLGANALLGVSMACAKAGAEYSGLPLYRYIGGTMANTLPVPMMNVLNGGAHADNTVD
FQEFMIMPVGFSSYSDALRSGAEIFHALKSLLKSKGLSTAVGDEGGFAPNLKSNEEAIELVIEAVGKAGYKIGSSTANGG
LGDAQVMIALDPASSEFYDAAKQKYVFKKSSKQELSSLEMAEYWEKWASDYPIISIEDGMAEDDWDGWKILTDKIGSRVQ
LVGDDLFVTNTSRLALGIERGVGNSILVKVNQIGTLTETLQAIDLARRNGYTAVISHRSGETEDSTIAQIAVATNAGQIK
TGSLSRSDRMAKYNELLRIEEELGLQAKYPALSAFRV
>Mature_436_residues
PIIRKILARQILDSRGNPTVEVDVYTENSFGRAAVPSGASTGVHEAVELRDGDSAVYLGKGVLKAVDHVNTVINDRLKGM
FVTDQEEIDMAMLALDGTPNKSRLGANALLGVSMACAKAGAEYSGLPLYRYIGGTMANTLPVPMMNVLNGGAHADNTVDF
QEFMIMPVGFSSYSDALRSGAEIFHALKSLLKSKGLSTAVGDEGGFAPNLKSNEEAIELVIEAVGKAGYKIGSSTANGGL
GDAQVMIALDPASSEFYDAAKQKYVFKKSSKQELSSLEMAEYWEKWASDYPIISIEDGMAEDDWDGWKILTDKIGSRVQL
VGDDLFVTNTSRLALGIERGVGNSILVKVNQIGTLTETLQAIDLARRNGYTAVISHRSGETEDSTIAQIAVATNAGQIKT
GSLSRSDRMAKYNELLRIEEELGLQAKYPALSAFRV

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=52.183908045977, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=51.0344827586207, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=50.3448275862069, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=50.3448275862069, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=45.4965357967667, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=348, Percent_Identity=22.7011494252874, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI169201757, Length=348, Percent_Identity=22.7011494252874, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI239744207, Length=348, Percent_Identity=22.7011494252874, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789141, Length=434, Percent_Identity=56.6820276497696, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71995829, Length=439, Percent_Identity=53.3029612756264, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=439, Percent_Identity=53.3029612756264, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=46.0732984293194, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=48.5193621867882, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=48.5193621867882, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=48.2915717539863, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321693, Length=438, Percent_Identity=46.5753424657534, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=438, Percent_Identity=47.0319634703196, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=443, Percent_Identity=51.4672686230248, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=443, Percent_Identity=51.4672686230248, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=443, Percent_Identity=51.4672686230248, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=443, Percent_Identity=51.4672686230248, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=443, Percent_Identity=51.4672686230248, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17137654, Length=443, Percent_Identity=51.4672686230248, Blast_Score=411, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CHLPD (A1BD13)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910714.1
- ProteinModelPortal:   A1BD13
- SMR:   A1BD13
- STRING:   A1BD13
- GeneID:   4570710
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0223
- NMPDR:   fig|290317.7.peg.235
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DYLATWV
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46923; Mature: 46792

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 349-349 BINDING 154-154 BINDING 163-163 BINDING 297-297 BINDING 324-324 BINDING 349-349 BINDING 400-400

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIRKILARQILDSRGNPTVEVDVYTENSFGRAAVPSGASTGVHEAVELRDGDSAVYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEC
KGVLKAVDHVNTVINDRLKGMFVTDQEEIDMAMLALDGTPNKSRLGANALLGVSMACAKA
HHHHHHHHHHHHHHHHHHCCEEECCHHHHCEEEEEECCCCCHHHCCCHHHHHHHHHHHHC
GAEYSGLPLYRYIGGTMANTLPVPMMNVLNGGAHADNTVDFQEFMIMPVGFSSYSDALRS
CCCCCCCCHHHHHCCHHHCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCCHHHHHHHH
GAEIFHALKSLLKSKGLSTAVGDEGGFAPNLKSNEEAIELVIEAVGKAGYKIGSSTANGG
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCC
LGDAQVMIALDPASSEFYDAAKQKYVFKKSSKQELSSLEMAEYWEKWASDYPIISIEDGM
CCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
AEDDWDGWKILTDKIGSRVQLVGDDLFVTNTSRLALGIERGVGNSILVKVNQIGTLTETL
CCCCCCCCCHHHHHCCCEEEEECCEEEEECCCCEEEEECCCCCCEEEEEEECCCHHHHHH
QAIDLARRNGYTAVISHRSGETEDSTIAQIAVATNAGQIKTGSLSRSDRMAKYNELLRIE
HHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHH
EELGLQAKYPALSAFRV
HHCCCEECCCCCHHCCC
>Mature Secondary Structure 
PIIRKILARQILDSRGNPTVEVDVYTENSFGRAAVPSGASTGVHEAVELRDGDSAVYLG
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEC
KGVLKAVDHVNTVINDRLKGMFVTDQEEIDMAMLALDGTPNKSRLGANALLGVSMACAKA
HHHHHHHHHHHHHHHHHHCCEEECCHHHHCEEEEEECCCCCHHHCCCHHHHHHHHHHHHC
GAEYSGLPLYRYIGGTMANTLPVPMMNVLNGGAHADNTVDFQEFMIMPVGFSSYSDALRS
CCCCCCCCHHHHHCCHHHCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCCHHHHHHHH
GAEIFHALKSLLKSKGLSTAVGDEGGFAPNLKSNEEAIELVIEAVGKAGYKIGSSTANGG
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCC
LGDAQVMIALDPASSEFYDAAKQKYVFKKSSKQELSSLEMAEYWEKWASDYPIISIEDGM
CCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
AEDDWDGWKILTDKIGSRVQLVGDDLFVTNTSRLALGIERGVGNSILVKVNQIGTLTETL
CCCCCCCCCHHHHHCCCEEEEECCEEEEECCCCEEEEECCCCCCEEEEEEECCCHHHHHH
QAIDLARRNGYTAVISHRSGETEDSTIAQIAVATNAGQIKTGSLSRSDRMAKYNELLRIE
HHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHH
EELGLQAKYPALSAFRV
HHCCCEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA