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The map label for this gene is gdhA [H]

Identifier: 119356014

GI number: 119356014

Start: 189446

End: 190774

Strand: Reverse

Name: gdhA [H]

Synonym: Cpha266_0167

Alternate gene names: 119356014

Gene position: 190774-189446 (Counterclockwise)

Preceding gene: 119356015

Following gene: 119356010

Centisome position: 6.09

GC content: 51.32

Gene sequence:

>1329_bases
ATGACAACATGGCCCCTTATGAGTGAACGAAAAGAGTCCTGCGAGTGCGCAATTCCTTATGACATCATAGAAACAGGCAT
CCATTCCAAACCAAACCCTTTTAAAACCGCCCAGAGGCAACTCGATGAAGCTGCTGCGCTCATCGGGCTTGATCCTGCCG
CCCATCAGCTTCTCCGCTGGCCGCTCAGAGAACTCCACGTCGCCATGCCGGTAAAAATGGACGACGGATCAACCAAAGTT
TTCAGAGGGTTCAGAGTGCAATACAACGATGCCCGCGGACCGAACAAGGGAGGCATACGGTTCCATGCTGAAGAAACCAT
CGACACCATCAGGGCTCTTGCCGCATGGATGACCTGGAAAACCGCCGTTCTCGACCTGCCGCTTGGCGGCGCAAAAGGCG
GCGTTATCTGCAATCCAAAATCGATGTCTCCCGGAGAACTCGAACGTCTCTCCCGAGCATATATCCGTCAGGCGGGAAGA
ATACTCGGACTTGAAAAAGACGTTGCTGCACCGGATATCTACACCACACCGCAGATTATGGCATGGATGGCCGATGAATA
CTCCTTTATGCAAGGCAACAACGAGTTTGGCGTTATTTCCGGAAAACCCCTCGCACTTGGCGGATCGGCCGGGAGAAACG
ACGCCACGGCAAGAGGAGGCATCGCTTGTGTCATAGAGGCGGCCAAAGAACTCGGCATTGAACTTCAAGGAGCTACGGCT
GCCATTCAGGGATATGGCAATGTCGGCTCGTTCGCGCACAAGCTTGCCACAGAACTGCTCGGTATGAAAATTATAGCCGT
TTCAGATTCCGGAGGAGGCATCTATAATCCTGACGGATTGCTGTATGATGAGGTCAAGGCCCATAAAGAGAAAACCGGAT
CGGTAACCGGCTTTCCGGGATCAGATTCTGTTTCTGACGAAACCCTGCTTGAACTGAACGTTACGGTACTCTTTCCCTCC
GCACTTGAGCAGGTAATCACCGAACACAACGCCCAAAACATCAGAGCAGGAATTATTGCAGAATTAGCCAATGGGCCAAC
AACACCCGAAGCCGATAAAATTCTTTTCGAAAACGGCTGTTATGTTATTCCCGACTTTCTCTGCAACGCAGGCGGAGTAA
CCGTTTCGTATTTCGAAATGGTTCAGAACACCTACGGCTACTACTGGGAAGAGGATGATGTTCGCAAGCGCCTTGAAAAG
AAAATGAAAACCGCGTTTCACAGCGTACACGAAACATCCCGGAAATACAAGGTGCACAACCGACTCGGAGCCTGCATTGT
CGCCATTGAACGAGTGGCAGAAGCCATGAAACTCAGAGGCTGGTATTAG

Upstream 100 bases:

>100_bases
ACGGCAAATTTGCCATTCCGGAGAATCCGAAAAAATAAAACCACTCCTGACCCGCACTCCCCTGATGAGTAAGCACAAGC
AATTGCAATAACGCCCCACA

Downstream 100 bases:

>100_bases
CAGTCCTCCCCAGACAATAACGCCCTGTTTATCCTGAAAATCAGCCCCTCACGCAAACCGTTACATCAAACAACAGCCGG
ATTGAAGCACTACTGAAAAG

Product: glutamate dehydrogenase (NAD/NADP)

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTWPLMSERKESCECAIPYDIIETGIHSKPNPFKTAQRQLDEAAALIGLDPAAHQLLRWPLRELHVAMPVKMDDGSTKV
FRGFRVQYNDARGPNKGGIRFHAEETIDTIRALAAWMTWKTAVLDLPLGGAKGGVICNPKSMSPGELERLSRAYIRQAGR
ILGLEKDVAAPDIYTTPQIMAWMADEYSFMQGNNEFGVISGKPLALGGSAGRNDATARGGIACVIEAAKELGIELQGATA
AIQGYGNVGSFAHKLATELLGMKIIAVSDSGGGIYNPDGLLYDEVKAHKEKTGSVTGFPGSDSVSDETLLELNVTVLFPS
ALEQVITEHNAQNIRAGIIAELANGPTTPEADKILFENGCYVIPDFLCNAGGVTVSYFEMVQNTYGYYWEEDDVRKRLEK
KMKTAFHSVHETSRKYKVHNRLGACIVAIERVAEAMKLRGWY

Sequences:

>Translated_442_residues
MTTWPLMSERKESCECAIPYDIIETGIHSKPNPFKTAQRQLDEAAALIGLDPAAHQLLRWPLRELHVAMPVKMDDGSTKV
FRGFRVQYNDARGPNKGGIRFHAEETIDTIRALAAWMTWKTAVLDLPLGGAKGGVICNPKSMSPGELERLSRAYIRQAGR
ILGLEKDVAAPDIYTTPQIMAWMADEYSFMQGNNEFGVISGKPLALGGSAGRNDATARGGIACVIEAAKELGIELQGATA
AIQGYGNVGSFAHKLATELLGMKIIAVSDSGGGIYNPDGLLYDEVKAHKEKTGSVTGFPGSDSVSDETLLELNVTVLFPS
ALEQVITEHNAQNIRAGIIAELANGPTTPEADKILFENGCYVIPDFLCNAGGVTVSYFEMVQNTYGYYWEEDDVRKRLEK
KMKTAFHSVHETSRKYKVHNRLGACIVAIERVAEAMKLRGWY
>Mature_441_residues
TTWPLMSERKESCECAIPYDIIETGIHSKPNPFKTAQRQLDEAAALIGLDPAAHQLLRWPLRELHVAMPVKMDDGSTKVF
RGFRVQYNDARGPNKGGIRFHAEETIDTIRALAAWMTWKTAVLDLPLGGAKGGVICNPKSMSPGELERLSRAYIRQAGRI
LGLEKDVAAPDIYTTPQIMAWMADEYSFMQGNNEFGVISGKPLALGGSAGRNDATARGGIACVIEAAKELGIELQGATAA
IQGYGNVGSFAHKLATELLGMKIIAVSDSGGGIYNPDGLLYDEVKAHKEKTGSVTGFPGSDSVSDETLLELNVTVLFPSA
LEQVITEHNAQNIRAGIIAELANGPTTPEADKILFENGCYVIPDFLCNAGGVTVSYFEMVQNTYGYYWEEDDVRKRLEKK
MKTAFHSVHETSRKYKVHNRLGACIVAIERVAEAMKLRGWY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=365, Percent_Identity=40.5479452054795, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI4885281, Length=365, Percent_Identity=40.5479452054795, Blast_Score=252, Evalue=5e-67,
Organism=Escherichia coli, GI1788059, Length=380, Percent_Identity=32.1052631578947, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17544676, Length=334, Percent_Identity=40.1197604790419, Blast_Score=232, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6319256, Length=421, Percent_Identity=28.5035629453682, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=416, Percent_Identity=28.3653846153846, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45549226, Length=332, Percent_Identity=38.855421686747, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45553475, Length=332, Percent_Identity=38.855421686747, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24649283, Length=337, Percent_Identity=36.2017804154303, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24649460, Length=327, Percent_Identity=36.085626911315, Blast_Score=184, Evalue=9e-47,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 48303; Mature: 48171

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTWPLMSERKESCECAIPYDIIETGIHSKPNPFKTAQRQLDEAAALIGLDPAAHQLLRW
CCCCCCCHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
PLRELHVAMPVKMDDGSTKVFRGFRVQYNDARGPNKGGIRFHAEETIDTIRALAAWMTWK
CHHHHHEEEEEEECCCCCEEEECEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
TAVLDLPLGGAKGGVICNPKSMSPGELERLSRAYIRQAGRILGLEKDVAAPDIYTTPQIM
HHEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHH
AWMADEYSFMQGNNEFGVISGKPLALGGSAGRNDATARGGIACVIEAAKELGIELQGATA
HHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCEE
AIQGYGNVGSFAHKLATELLGMKIIAVSDSGGGIYNPDGLLYDEVKAHKEKTGSVTGFPG
EEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCCCCCC
SDSVSDETLLELNVTVLFPSALEQVITEHNAQNIRAGIIAELANGPTTPEADKILFENGC
CCCCCCCEEEEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCC
YVIPDFLCNAGGVTVSYFEMVQNTYGYYWEEDDVRKRLEKKMKTAFHSVHETSRKYKVHN
EEECHHHCCCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGACIVAIERVAEAMKLRGWY
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTWPLMSERKESCECAIPYDIIETGIHSKPNPFKTAQRQLDEAAALIGLDPAAHQLLRW
CCCCCCHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
PLRELHVAMPVKMDDGSTKVFRGFRVQYNDARGPNKGGIRFHAEETIDTIRALAAWMTWK
CHHHHHEEEEEEECCCCCEEEECEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
TAVLDLPLGGAKGGVICNPKSMSPGELERLSRAYIRQAGRILGLEKDVAAPDIYTTPQIM
HHEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHH
AWMADEYSFMQGNNEFGVISGKPLALGGSAGRNDATARGGIACVIEAAKELGIELQGATA
HHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCEE
AIQGYGNVGSFAHKLATELLGMKIIAVSDSGGGIYNPDGLLYDEVKAHKEKTGSVTGFPG
EEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCCCCCC
SDSVSDETLLELNVTVLFPSALEQVITEHNAQNIRAGIIAELANGPTTPEADKILFENGC
CCCCCCCEEEEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCC
YVIPDFLCNAGGVTVSYFEMVQNTYGYYWEEDDVRKRLEKKMKTAFHSVHETSRKYKVHN
EEECHHHCCCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGACIVAIERVAEAMKLRGWY
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8135516 [H]