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The map label for this gene is trmE

Identifier: 119356003

GI number: 119356003

Start: 172835

End: 174262

Strand: Direct

Name: trmE

Synonym: Cpha266_0153

Alternate gene names: 119356003

Gene position: 172835-174262 (Clockwise)

Preceding gene: 119356002

Following gene: 119356004

Centisome position: 5.52

GC content: 59.17

Gene sequence:

>1428_bases
ATGCAGCAGGAGACCGGCATCCACCCGATAGTTCAGTGCGACCCCATTGCCGCCATTGCCACCCCTCTCGGCGTTGGCGC
TCTTGCCGTTGTGCGTATCAGCGGCGCAACGGCCTTCGATATTGCAGGTCGTGTTTTCAGAAAAGCCCGCGATCCGCACC
AGCCCCTCTCCGAAACGCCCGGATATACCGCTCATTTCGGCAGGTTGTACGACGGCCTGACGCTGATCGACGAGGTGATC
GCGCTGGTGTTCCGTTCTCCCTCCTCCTTTACGGCCGAGGATATGGTCGAATTCACCTGCCATGGAGGCCCGGTTGTAAC
CCGGCATGTTCTCCAGCTTCTGCTTGACAACGGATGCCGACTGGCGGAACCCGGAGAGTTCACCCGCAGAGCCTTTCTCA
ACGGCAAGATCGATCTTCTCCAGGCCGAGGCTATCGGAGAGATGATTCACGCCCGTTCGGAATCGGCATACCGCACCGCC
GTTATCCAGATGAAGGGCGATCTGTCGGCAACCCTCGGAGCCCTGCGCGAAAAGCTGCTCCGCTCCTGCGCCCTTCTTGA
GCTCGAACTGGATTTCAGCGAAGAGGATGTCGAGTTCCAGAGCCGCGAAGAGCTGAGGGTTGAGATTGCCTCATTGCAGA
GCGAGGTTGCCAGGCTTGTTGATTCCTACCAGCACGGCAGGCTGCTCACCGAAGGCGTGGCCACGGTGATCGCCGGCAGG
CCGAATGCCGGAAAGTCAACGCTGCTCAACACCCTGCTCGGCGAAGAGCGGGCCATCGTGAGCCACATGCCCGGCACCAC
GCGTGACTATATCGAAGAGTGCTTTATTCACGATAAAACCATGTTCCGCCTTACCGATACCGCGGGTTTGCGTGAAACCG
GGGAGGAGATCGAGCACGAAGGCGTTCGCCGCAGCCGGATGAAAATGGCCGAAGCCGATCTGCTGCTCTATCTCCTTGAT
CTCAGCCGGGAGGGCATGGCAGAGGAGATCAAGGAGATCGTCGCTTTGAAAGCAGCCCATGCGTCCTCGAAATTTCTGGT
GGTCGCCAACAAGATCGACCTTGTGCCCGACGCGACAACCCTGCTTTGCAGGCTCGGCGAGGAGGCCGGCTGCGAGGCGA
TCGGTATTTCAGCGAGGCAGAAGCTCGGCATTGACGAGCTGAAAAGCCGTATGAGCAGTATGGTTGAGGGGCTCGACAAG
CTGCACGAAGCAAGCGTGCTCGTAACCAGCCTTCGCCACTACGAAGCGCTGCGCAACGCCGGCGATGCGCTCCGAAACGC
GCAGAATCTCCTGGACGAACAGGCGGATACCGAGCTGATCGCCTTCGAACTGCGCGCAGCACTCGACTATGTTGGCGAGA
TCACCGGAAAGGTGGTCAATGAAGAGATTCTTCATGCGATTTTCGAGCGGTTCTGCATCGGCAAGTAA

Upstream 100 bases:

>100_bases
CCCATCTGGCAGGGTTTCTTGTTTCGATCATTGAACTGCCGGACAGCATGCTGCCCGACCAGATCACCGTAAGGCCCTTG
TAACCATTTAAGGATAACTC

Downstream 100 bases:

>100_bases
ATAAGGGAAGGGTGAACAGTATCAGAGAAGTCGGAAGAGAGGTGTTTTGTTCCTGAATTCATTATGTTTTAACCTTGCAA
ATCATGACGCCCAAATAAAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MQQETGIHPIVQCDPIAAIATPLGVGALAVVRISGATAFDIAGRVFRKARDPHQPLSETPGYTAHFGRLYDGLTLIDEVI
ALVFRSPSSFTAEDMVEFTCHGGPVVTRHVLQLLLDNGCRLAEPGEFTRRAFLNGKIDLLQAEAIGEMIHARSESAYRTA
VIQMKGDLSATLGALREKLLRSCALLELELDFSEEDVEFQSREELRVEIASLQSEVARLVDSYQHGRLLTEGVATVIAGR
PNAGKSTLLNTLLGEERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLRETGEEIEHEGVRRSRMKMAEADLLLYLLD
LSREGMAEEIKEIVALKAAHASSKFLVVANKIDLVPDATTLLCRLGEEAGCEAIGISARQKLGIDELKSRMSSMVEGLDK
LHEASVLVTSLRHYEALRNAGDALRNAQNLLDEQADTELIAFELRAALDYVGEITGKVVNEEILHAIFERFCIGK

Sequences:

>Translated_475_residues
MQQETGIHPIVQCDPIAAIATPLGVGALAVVRISGATAFDIAGRVFRKARDPHQPLSETPGYTAHFGRLYDGLTLIDEVI
ALVFRSPSSFTAEDMVEFTCHGGPVVTRHVLQLLLDNGCRLAEPGEFTRRAFLNGKIDLLQAEAIGEMIHARSESAYRTA
VIQMKGDLSATLGALREKLLRSCALLELELDFSEEDVEFQSREELRVEIASLQSEVARLVDSYQHGRLLTEGVATVIAGR
PNAGKSTLLNTLLGEERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLRETGEEIEHEGVRRSRMKMAEADLLLYLLD
LSREGMAEEIKEIVALKAAHASSKFLVVANKIDLVPDATTLLCRLGEEAGCEAIGISARQKLGIDELKSRMSSMVEGLDK
LHEASVLVTSLRHYEALRNAGDALRNAQNLLDEQADTELIAFELRAALDYVGEITGKVVNEEILHAIFERFCIGK
>Mature_475_residues
MQQETGIHPIVQCDPIAAIATPLGVGALAVVRISGATAFDIAGRVFRKARDPHQPLSETPGYTAHFGRLYDGLTLIDEVI
ALVFRSPSSFTAEDMVEFTCHGGPVVTRHVLQLLLDNGCRLAEPGEFTRRAFLNGKIDLLQAEAIGEMIHARSESAYRTA
VIQMKGDLSATLGALREKLLRSCALLELELDFSEEDVEFQSREELRVEIASLQSEVARLVDSYQHGRLLTEGVATVIAGR
PNAGKSTLLNTLLGEERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLRETGEEIEHEGVRRSRMKMAEADLLLYLLD
LSREGMAEEIKEIVALKAAHASSKFLVVANKIDLVPDATTLLCRLGEEAGCEAIGISARQKLGIDELKSRMSSMVEGLDK
LHEASVLVTSLRHYEALRNAGDALRNAQNLLDEQADTELIAFELRAALDYVGEITGKVVNEEILHAIFERFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=482, Percent_Identity=34.4398340248963, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI193082988, Length=514, Percent_Identity=32.1011673151751, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI193082993, Length=475, Percent_Identity=33.6842105263158, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI2367268, Length=470, Percent_Identity=37.0212765957447, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87082120, Length=124, Percent_Identity=35.4838709677419, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=30.0429184549356, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=505, Percent_Identity=33.0693069306931, Blast_Score=217, Evalue=4e-57,
Organism=Drosophila melanogaster, GI45550813, Length=428, Percent_Identity=32.9439252336449, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHLPD (A1BCU6)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910647.1
- ProteinModelPortal:   A1BCU6
- SMR:   A1BCU6
- STRING:   A1BCU6
- GeneID:   4568794
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0153
- NMPDR:   fig|290317.7.peg.161
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 52220; Mature: 52220

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 97-97 BINDING 136-136 BINDING 475-475

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQETGIHPIVQCDPIAAIATPLGVGALAVVRISGATAFDIAGRVFRKARDPHQPLSETP
CCCCCCCCCCEECCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCHHCCC
GYTAHFGRLYDGLTLIDEVIALVFRSPSSFTAEDMVEFTCHGGPVVTRHVLQLLLDNGCR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
LAEPGEFTRRAFLNGKIDLLQAEAIGEMIHARSESAYRTAVIQMKGDLSATLGALREKLL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHHHHHH
RSCALLELELDFSEEDVEFQSREELRVEIASLQSEVARLVDSYQHGRLLTEGVATVIAGR
HHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
PNAGKSTLLNTLLGEERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLRETGEEIEHE
CCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHH
GVRRSRMKMAEADLLLYLLDLSREGMAEEIKEIVALKAAHASSKFLVVANKIDLVPDATT
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
LLCRLGEEAGCEAIGISARQKLGIDELKSRMSSMVEGLDKLHEASVLVTSLRHYEALRNA
HHHHCCHHCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDALRNAQNLLDEQADTELIAFELRAALDYVGEITGKVVNEEILHAIFERFCIGK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQETGIHPIVQCDPIAAIATPLGVGALAVVRISGATAFDIAGRVFRKARDPHQPLSETP
CCCCCCCCCCEECCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCHHCCC
GYTAHFGRLYDGLTLIDEVIALVFRSPSSFTAEDMVEFTCHGGPVVTRHVLQLLLDNGCR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
LAEPGEFTRRAFLNGKIDLLQAEAIGEMIHARSESAYRTAVIQMKGDLSATLGALREKLL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHHHHHH
RSCALLELELDFSEEDVEFQSREELRVEIASLQSEVARLVDSYQHGRLLTEGVATVIAGR
HHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
PNAGKSTLLNTLLGEERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLRETGEEIEHE
CCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHH
GVRRSRMKMAEADLLLYLLDLSREGMAEEIKEIVALKAAHASSKFLVVANKIDLVPDATT
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
LLCRLGEEAGCEAIGISARQKLGIDELKSRMSSMVEGLDKLHEASVLVTSLRHYEALRNA
HHHHCCHHCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDALRNAQNLLDEQADTELIAFELRAALDYVGEITGKVVNEEILHAIFERFCIGK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA