Click here to switch to the map view.

The map label for this gene is hmeA [H]

Identifier: 119355989

GI number: 119355989

Start: 158732

End: 159508

Strand: Direct

Name: hmeA [H]

Synonym: Cpha266_0139

Alternate gene names: 119355989

Gene position: 158732-159508 (Clockwise)

Preceding gene: 119355988

Following gene: 119355990

Centisome position: 5.06

GC content: 51.35

Gene sequence:

>777_bases
ATGAACGAACAAAGAAGAGATTTTATCAGGAAAACAGGCCTTGGGGTTCTTGCAGGTCTTGGCGTTGCTTCAGGGCTGTT
TGCGGAATCATCCTTTGAAAAAACGGTTCTGCCTGCATGGGACGAAAAGCCTGTTGCGGGAAAAATCCGCTGGGGTATGC
TTGTTGACACCCGCACCTGCAACGAGAGTTGTCAGGAGTGCGTTCTGACGTGTCATCACGTTCACAACGTTCCGGATTTC
GGGAGTCGCAAGGATGAGGTCAAGTGGATCTGGAAGAGTCCTTATGAAAAGGCTTTTCCTACCGCGAGCCAGCAGTTTCA
GAATAAAGAGACCAGGGATCGCTCCTGTTTGACGCTTTGCAACCATTGCGCTGAATCGCCATGTACAAAAGCCTGCCCTA
CGGAGGCAACCTTCAAGCGGTGGGATGGTATTGTGGCGATGGATTACCATCGATGCATCGGATGCCGCTTTTGCATGGCG
GCATGCCCATACGGTTCACGGAGCTTCAACTGGCAGGATCCCCGGATGGCGATCAAAAATCCGAGCGCCGATTACCCTAC
AAGAATGCAGGGCGTTGTTGAAAAATGCAATTTCTGTTCAGAACGGCTCGTAAAGGGACTCAAGCCAGCCTGCGTTGAGA
GCTGCCCGAATCAGGCTCTGACCTTTGGCGATCTCAACGATCCGGAGTCTGACATCCGGAAGCTTCTTGAAAACAACCAG
ACGATGCAGCGGAAACCCGAACTGGGTACCTTGCCGTCAGTTTTTTACATTATCTAA

Upstream 100 bases:

>100_bases
GTCATGCCAATAACCGGCTATTCTGCTTTAACTGCCACATGTATGCCAATGTCAAGCCGAAGTGCTGGAATTGTCATATA
TCGCCTATGGAGACACCGTG

Downstream 100 bases:

>100_bases
CGACTTCCCATGATAGAGAAAGCGCTGAAAGGAAACCGAAACTACTGGCTCTGGATAGCATTCCTTTGTCTGGTTATTGG
TTCCGGACTTTTTGCCTACA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNEQRRDFIRKTGLGVLAGLGVASGLFAESSFEKTVLPAWDEKPVAGKIRWGMLVDTRTCNESCQECVLTCHHVHNVPDF
GSRKDEVKWIWKSPYEKAFPTASQQFQNKETRDRSCLTLCNHCAESPCTKACPTEATFKRWDGIVAMDYHRCIGCRFCMA
ACPYGSRSFNWQDPRMAIKNPSADYPTRMQGVVEKCNFCSERLVKGLKPACVESCPNQALTFGDLNDPESDIRKLLENNQ
TMQRKPELGTLPSVFYII

Sequences:

>Translated_258_residues
MNEQRRDFIRKTGLGVLAGLGVASGLFAESSFEKTVLPAWDEKPVAGKIRWGMLVDTRTCNESCQECVLTCHHVHNVPDF
GSRKDEVKWIWKSPYEKAFPTASQQFQNKETRDRSCLTLCNHCAESPCTKACPTEATFKRWDGIVAMDYHRCIGCRFCMA
ACPYGSRSFNWQDPRMAIKNPSADYPTRMQGVVEKCNFCSERLVKGLKPACVESCPNQALTFGDLNDPESDIRKLLENNQ
TMQRKPELGTLPSVFYII
>Mature_258_residues
MNEQRRDFIRKTGLGVLAGLGVASGLFAESSFEKTVLPAWDEKPVAGKIRWGMLVDTRTCNESCQECVLTCHHVHNVPDF
GSRKDEVKWIWKSPYEKAFPTASQQFQNKETRDRSCLTLCNHCAESPCTKACPTEATFKRWDGIVAMDYHRCIGCRFCMA
ACPYGSRSFNWQDPRMAIKNPSADYPTRMQGVVEKCNFCSERLVKGLKPACVESCPNQALTFGDLNDPESDIRKLLENNQ
TMQRKPELGTLPSVFYII

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=259, Percent_Identity=32.8185328185328, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI226510944, Length=256, Percent_Identity=30.859375, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787122, Length=178, Percent_Identity=32.0224719101124, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787872, Length=178, Percent_Identity=32.0224719101124, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789370, Length=249, Percent_Identity=29.3172690763052, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790326, Length=176, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787749, Length=185, Percent_Identity=27.5675675675676, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082114, Length=125, Percent_Identity=34.4, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787740, Length=108, Percent_Identity=35.1851851851852, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788811, Length=160, Percent_Identity=28.125, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787478, Length=108, Percent_Identity=35.1851851851852, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=32.4137931034483, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789067, Length=113, Percent_Identity=36.283185840708, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789079, Length=150, Percent_Identity=27.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082180, Length=105, Percent_Identity=32.3809523809524, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29208; Mature: 29208

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQRRDFIRKTGLGVLAGLGVASGLFAESSFEKTVLPAWDEKPVAGKIRWGMLVDTRTC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECEEECCHHH
NESCQECVLTCHHVHNVPDFGSRKDEVKWIWKSPYEKAFPTASQQFQNKETRDRSCLTLC
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHCCCHHHHHHCHHHHHHHHHHHH
NHCAESPCTKACPTEATFKRWDGIVAMDYHRCIGCRFCMAACPYGSRSFNWQDPRMAIKN
HHHHCCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECC
PSADYPTRMQGVVEKCNFCSERLVKGLKPACVESCPNQALTFGDLNDPESDIRKLLENNQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCHHHHHHHHHCCC
TMQRKPELGTLPSVFYII
HHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MNEQRRDFIRKTGLGVLAGLGVASGLFAESSFEKTVLPAWDEKPVAGKIRWGMLVDTRTC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECEEECCHHH
NESCQECVLTCHHVHNVPDFGSRKDEVKWIWKSPYEKAFPTASQQFQNKETRDRSCLTLC
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHCCCHHHHHHCHHHHHHHHHHHH
NHCAESPCTKACPTEATFKRWDGIVAMDYHRCIGCRFCMAACPYGSRSFNWQDPRMAIKN
HHHHCCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECC
PSADYPTRMQGVVEKCNFCSERLVKGLKPACVESCPNQALTFGDLNDPESDIRKLLENNQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCHHHHHHHHHCCC
TMQRKPELGTLPSVFYII
HHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]