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The map label for this gene is ecoRIIM [H]

Identifier: 119355933

GI number: 119355933

Start: 97588

End: 98865

Strand: Reverse

Name: ecoRIIM [H]

Synonym: Cpha266_0083

Alternate gene names: 119355933

Gene position: 98865-97588 (Counterclockwise)

Preceding gene: 119355934

Following gene: 119355932

Centisome position: 3.15

GC content: 49.22

Gene sequence:

>1278_bases
ATGAACACGACCCAGAGCTTTTCAGTGTTACGCCAGCAGGCAGGTTTGTCGATTGACAAAATTGCTCATATCACCGGATA
CAGCCAGAGAGAGGTTTACCGCTGGGAAAAAAACGAGGTTGTGCCCCGAAAAATGGTGCTTGACCAGCTCACAAGAATTG
CCCAGCCTTTATCAACCTATGGAAAAGACTCTTCCAGCTTTACCTTCATTGATCTTTTTGCAGGTATTGGAGGCATGAGA
AAAGCCTTTGAGGAGCTTGGCGGCAGATGCGTGTTTACAAGCGAATGGGACAAGTATTCCCGTCAGACCTACTGTGCAAA
TTTTGATTGTGACCACGAGATTGGCGGCGACATCACAAAAATTGATGCCCGCGACATTCCGTCACATGATATTCTGGTTG
CGGGCTTTCCCTGCCAGCCATTTTCAATAGCCGGAGTCTCCAAAAAAAATGCTCTCGGCAAGCCTCACGGATTCGCCGAC
AAAACCCAGGGCACCCTCTTTTTTGATGTGGCAAGGGTTATTGAGTACCATCAACCGAAGGCGATCCTGCTTGAAAATGT
CAAAAACCTTCAGAGGCATGACAAGGGGAAAACATTTGCAGTTATCAAGGAAACGCTCACGGAACTGGGCTATTACTTTG
ACTGGAAAATCATTGATGCCGGAAACTGGGTTCCCCAACACCGGGAGCGAATATTTATCGCCGGATTCAGGGAGGATTGC
GGATTCAGCTTTGACCGGTTTGATCTGCCACACGGCAACGAGCGCCCGGTCATAGAAACCATATTGCACCCTGAAAACGG
TATGGAAGATGAGGAGCCCCCATATACAGTCGGTCCGTTGGCAAGAATATCCGACCGCTATACCCTTTCGGATCATCTCT
GGGAGTATCTGCAGAAGTATGCAGAAAAACATCGGGCAAAAGGCAATGGTTTCGGATTCGGCCTTGTCGGTCCACACGAT
GTAGCCAGAACACTTTCGGCACGGTATTACAAGGATGGTTCCGAGATACTGGTGAAGCAGGAGGGGAAAAATCCACGCAG
ACTGACACCGAGGGAGTGTGCACGGTTGATGGGTTTTGACACGCCCAATGGCAACAGTTTTGTGATTCCTGTTTCGGATA
CCCAGGCTTACCGGCAGTTCGGCAATGCAGTGGTTGTACCTGTCGTCATGTCAGTAGCCCGGTATATGCTTGAATATCTG
ATGGATGGTGTTTCATCCGACCAGTTGCGTTTTGACTTTGGCATTCTGATGGCGGACAACGCTGTACAGTATGGCTGA

Upstream 100 bases:

>100_bases
CCTTTTTACCTTTTCTGCTTCATGATTTTCATGCCGGGATTTACGGGTTATTGCTTATACTTGTCATAATGTCAAAATAT
TTCCCGTGGAGGAGAGGATC

Downstream 100 bases:

>100_bases
TGTTGTTTCGCCGGAAACACGAAGCCGCATGATGTCCGGCATCCGGTCAGAGAATACCGTTCCCGAAATGAGGGTTCGCA
GAGGTCTTCATGCAAGGGGG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNTTQSFSVLRQQAGLSIDKIAHITGYSQREVYRWEKNEVVPRKMVLDQLTRIAQPLSTYGKDSSSFTFIDLFAGIGGMR
KAFEELGGRCVFTSEWDKYSRQTYCANFDCDHEIGGDITKIDARDIPSHDILVAGFPCQPFSIAGVSKKNALGKPHGFAD
KTQGTLFFDVARVIEYHQPKAILLENVKNLQRHDKGKTFAVIKETLTELGYYFDWKIIDAGNWVPQHRERIFIAGFREDC
GFSFDRFDLPHGNERPVIETILHPENGMEDEEPPYTVGPLARISDRYTLSDHLWEYLQKYAEKHRAKGNGFGFGLVGPHD
VARTLSARYYKDGSEILVKQEGKNPRRLTPRECARLMGFDTPNGNSFVIPVSDTQAYRQFGNAVVVPVVMSVARYMLEYL
MDGVSSDQLRFDFGILMADNAVQYG

Sequences:

>Translated_425_residues
MNTTQSFSVLRQQAGLSIDKIAHITGYSQREVYRWEKNEVVPRKMVLDQLTRIAQPLSTYGKDSSSFTFIDLFAGIGGMR
KAFEELGGRCVFTSEWDKYSRQTYCANFDCDHEIGGDITKIDARDIPSHDILVAGFPCQPFSIAGVSKKNALGKPHGFAD
KTQGTLFFDVARVIEYHQPKAILLENVKNLQRHDKGKTFAVIKETLTELGYYFDWKIIDAGNWVPQHRERIFIAGFREDC
GFSFDRFDLPHGNERPVIETILHPENGMEDEEPPYTVGPLARISDRYTLSDHLWEYLQKYAEKHRAKGNGFGFGLVGPHD
VARTLSARYYKDGSEILVKQEGKNPRRLTPRECARLMGFDTPNGNSFVIPVSDTQAYRQFGNAVVVPVVMSVARYMLEYL
MDGVSSDQLRFDFGILMADNAVQYG
>Mature_425_residues
MNTTQSFSVLRQQAGLSIDKIAHITGYSQREVYRWEKNEVVPRKMVLDQLTRIAQPLSTYGKDSSSFTFIDLFAGIGGMR
KAFEELGGRCVFTSEWDKYSRQTYCANFDCDHEIGGDITKIDARDIPSHDILVAGFPCQPFSIAGVSKKNALGKPHGFAD
KTQGTLFFDVARVIEYHQPKAILLENVKNLQRHDKGKTFAVIKETLTELGYYFDWKIIDAGNWVPQHRERIFIAGFREDC
GFSFDRFDLPHGNERPVIETILHPENGMEDEEPPYTVGPLARISDRYTLSDHLWEYLQKYAEKHRAKGNGFGFGLVGPHD
VARTLSARYYKDGSEILVKQEGKNPRRLTPRECARLMGFDTPNGNSFVIPVSDTQAYRQFGNAVVVPVVMSVARYMLEYL
MDGVSSDQLRFDFGILMADNAVQYG

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=370, Percent_Identity=48.9189189189189, Blast_Score=331, Evalue=6e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48219; Mature: 48219

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTQSFSVLRQQAGLSIDKIAHITGYSQREVYRWEKNEVVPRKMVLDQLTRIAQPLSTY
CCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
GKDSSSFTFIDLFAGIGGMRKAFEELGGRCVFTSEWDKYSRQTYCANFDCDHEIGGDITK
CCCCCCEEHHHHHHCCCHHHHHHHHHCCEEEEECCHHHHCCCEEEECCCCCCCCCCCEEE
IDARDIPSHDILVAGFPCQPFSIAGVSKKNALGKPHGFADKTQGTLFFDVARVIEYHQPK
ECCCCCCCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
AILLENVKNLQRHDKGKTFAVIKETLTELGYYFDWKIIDAGNWVPQHRERIFIAGFREDC
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEECHHHH
GFSFDRFDLPHGNERPVIETILHPENGMEDEEPPYTVGPLARISDRYTLSDHLWEYLQKY
CCCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
AEKHRAKGNGFGFGLVGPHDVARTLSARYYKDGSEILVKQEGKNPRRLTPRECARLMGFD
HHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCC
TPNGNSFVIPVSDTQAYRQFGNAVVVPVVMSVARYMLEYLMDGVSSDQLRFDFGILMADN
CCCCCEEEEECCCHHHHHHHCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
AVQYG
CCCCC
>Mature Secondary Structure
MNTTQSFSVLRQQAGLSIDKIAHITGYSQREVYRWEKNEVVPRKMVLDQLTRIAQPLSTY
CCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
GKDSSSFTFIDLFAGIGGMRKAFEELGGRCVFTSEWDKYSRQTYCANFDCDHEIGGDITK
CCCCCCEEHHHHHHCCCHHHHHHHHHCCEEEEECCHHHHCCCEEEECCCCCCCCCCCEEE
IDARDIPSHDILVAGFPCQPFSIAGVSKKNALGKPHGFADKTQGTLFFDVARVIEYHQPK
ECCCCCCCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
AILLENVKNLQRHDKGKTFAVIKETLTELGYYFDWKIIDAGNWVPQHRERIFIAGFREDC
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEECHHHH
GFSFDRFDLPHGNERPVIETILHPENGMEDEEPPYTVGPLARISDRYTLSDHLWEYLQKY
CCCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
AEKHRAKGNGFGFGLVGPHDVARTLSARYYKDGSEILVKQEGKNPRRLTPRECARLMGFD
HHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCC
TPNGNSFVIPVSDTQAYRQFGNAVVVPVVMSVARYMLEYLMDGVSSDQLRFDFGILMADN
CCCCCEEEEECCCHHHHHHHCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
AVQYG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]