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The map label for this gene is vsr [H]

Identifier: 119355932

GI number: 119355932

Start: 97065

End: 97595

Strand: Reverse

Name: vsr [H]

Synonym: Cpha266_0082

Alternate gene names: 119355932

Gene position: 97595-97065 (Counterclockwise)

Preceding gene: 119355933

Following gene: 119355931

Centisome position: 3.11

GC content: 50.28

Gene sequence:

>531_bases
ATGGCTGATGTTGTTTCGCCGGAAACACGAAGCCGCATGATGTCCGGCATCCGGTCAGAGAATACCGTTCCCGAAATGAG
GGTTCGCAGAGGTCTTCATGCAAGGGGGTTCAGATTCAGGCTCCATTGCGGGGGATTACCCGGCAAGCCGGATATCGTTT
TTCCGAAGTATCGTGCCGTTATTTTTATACATGGATGTTTCTGGCATGCCCACGAGTGCCCTCTCTTCAAATGGCCGAAA
ACAAGGGAGGATTTCTGGAAAAAGAAGATCGGCAAAAACAGAATCCGTGACCTTGCTGTCATGGATGAACTTGCACGACA
AGGCTGGAGAGTTCTGATTGTCTGGGAGTGTGCCCTGAAAGGCAAGGGGCGCAGGCCGATCGATGAAGTTGTTGATTCCT
GTGCAGAGTGGCTGCTTTCAAGCTCGACAAAACGTGAAATCAGAGGGCAACATGAGATTATCGACATTACAATCGATCAT
GAAAAACAAGGGTTGCCGGACGATCTACGCGAAGGTACTCGCCGCAAATGA

Upstream 100 bases:

>100_bases
TCAGTAGCCCGGTATATGCTTGAATATCTGATGGATGGTGTTTCATCCGACCAGTTGCGTTTTGACTTTGGCATTCTGAT
GGCGGACAACGCTGTACAGT

Downstream 100 bases:

>100_bases
CAACAGAAAGCAGCAGGTCTATTTCGGCGGTGATTTCCAGGCTATCAATATTATTCCCTTTGACACGATAGCTCCTGATC
CCGACAAGCCTCATATTTTC

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: DNA mismatch endonuclease; V.EcoKDcm; Vsr mismatch endonuclease [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MADVVSPETRSRMMSGIRSENTVPEMRVRRGLHARGFRFRLHCGGLPGKPDIVFPKYRAVIFIHGCFWHAHECPLFKWPK
TREDFWKKKIGKNRIRDLAVMDELARQGWRVLIVWECALKGKGRRPIDEVVDSCAEWLLSSSTKREIRGQHEIIDITIDH
EKQGLPDDLREGTRRK

Sequences:

>Translated_176_residues
MADVVSPETRSRMMSGIRSENTVPEMRVRRGLHARGFRFRLHCGGLPGKPDIVFPKYRAVIFIHGCFWHAHECPLFKWPK
TREDFWKKKIGKNRIRDLAVMDELARQGWRVLIVWECALKGKGRRPIDEVVDSCAEWLLSSSTKREIRGQHEIIDITIDH
EKQGLPDDLREGTRRK
>Mature_175_residues
ADVVSPETRSRMMSGIRSENTVPEMRVRRGLHARGFRFRLHCGGLPGKPDIVFPKYRAVIFIHGCFWHAHECPLFKWPKT
REDFWKKKIGKNRIRDLAVMDELARQGWRVLIVWECALKGKGRRPIDEVVDSCAEWLLSSSTKREIRGQHEIIDITIDHE
KQGLPDDLREGTRRK

Specific function: Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-C transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=150, Percent_Identity=45.3333333333333, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 20584; Mature: 20453

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVVSPETRSRMMSGIRSENTVPEMRVRRGLHARGFRFRLHCGGLPGKPDIVFPKYRAV
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCEEE
IFIHGCFWHAHECPLFKWPKTREDFWKKKIGKNRIRDLAVMDELARQGWRVLIVWECALK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
GKGRRPIDEVVDSCAEWLLSSSTKREIRGQHEIIDITIDHEKQGLPDDLREGTRRK
CCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
ADVVSPETRSRMMSGIRSENTVPEMRVRRGLHARGFRFRLHCGGLPGKPDIVFPKYRAV
CCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCEEE
IFIHGCFWHAHECPLFKWPKTREDFWKKKIGKNRIRDLAVMDELARQGWRVLIVWECALK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
GKGRRPIDEVVDSCAEWLLSSSTKREIRGQHEIIDITIDHEKQGLPDDLREGTRRK
CCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2527357; 2198248; 9097040; 9278503; 1944537; 10871403; 10612397; 10360178 [H]