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The map label for this gene is pckG [H]

Identifier: 119355901

GI number: 119355901

Start: 52835

End: 54700

Strand: Direct

Name: pckG [H]

Synonym: Cpha266_0051

Alternate gene names: 119355901

Gene position: 52835-54700 (Clockwise)

Preceding gene: 119355900

Following gene: 119355902

Centisome position: 1.69

GC content: 52.95

Gene sequence:

>1866_bases
ATGACTTTGGTAGATATTAACGCACCTGACTTTGTCGTCAACAAAAAGTTGCTGCAATGGGTTCAGGAAACCGCAGACCT
TTGCCGCCCCGATCTGGTGCATTGGTGTGATGGGTCACAGGAAGAGTACGATCTTCTTTGCGAGCAGATGGTTGAGAGCG
GAACCTTTATCAGGCTCTCTGCTGAAAAGCGACCGAACAGTTTTTTGTGCCGTTCCGATCCGAGCGATGTCGCGAGGGTA
GAAGACAGGACATATATATGCAGTATCCGCAAGCAGGATGCAGGCCCGACCAACAACTGGGTCGCGCCCAAAGAGATGAA
AAAGATTCTTGCCGGACTGTATGATGGTTGCATGCAAGGCAGAACCATGTACATTATTCCGTTCAGCATGGGCCCTCTCG
GGTCGCATATTGCCCATATCGGCGTTGAAATAACCGATTCGCCCTATGTGGTCACCAACATGCGTATCATGACGAGAATG
GGACGCAAGGTTCTCGATGTTCTGGGCGCGAACGGCGATTTTGTTCATTGTCTGCACTCTGTAGGAGCTCCGCTCAAAGC
GGGCGATCAGGATGTTTCCTGGCCTTGCAGTGAAACGAAGTACATCGTGCATTTTCCGGAAGAGCGTTCGATCATGTCGT
TCGGAAGTGGCTATGGCGGCAACGCCCTTCTCGGGAAAAAATGTTTTGCGCTGCGCATTGCTTCCGCGATGGCTCGCGAT
GAAGGGTGGCTGGCCGAGCATATGCTGATTCTTGGCGTTGAGTCGCCGGATGGAGAAAAAACCTATGTTTCGGCAGCGTT
TCCGAGTGCCTGCGGGAAAACCAACTTCGCAATGCTTATTCCGCCGGAATCATTTGCCGGATGGAAAGTCACAACGATAG
GCGACGATATTGCCTGGATCAAGCCGGGCGAGGATGGGCGCCTTCATGCCATCAACCCTGAATATGGCTTTTTCGGCGTC
GCTCCCGGAACCTCGGATAAGTCCAATCCGAACGCCATGGAAACTCTCAAAAAGAACTGCATTTTTACAAATGTCGCGCT
CACGCCCGACGGCGATGTGTGGTGGGAGGGAATGACTGACGATATTCCCGAAGAGCTGACCGACTGGCGGGGGAATCCCT
GGACTCCCGGATGCGGAAGACCTTCTTCACATCCCAATGCCCGTTTTACGGCTCCCGCAAGTCAGTGTCCGTCGATCGAT
CCTGACTGGGAAAACCCTCACGGGGTTCCGATCAGCGCATTCATTTTCGGCGGTCGCCGCAGAGATACGATTCCGCTTGT
CTATCAATCGGCAAACTGGTATTTCGGCGTCTATATGGCCGCGACAATGGGTTCGGAGATGACCGCAGCCGCTGCCGGTC
TGATCGGGGATGTTCGCAGGGATCCTTATGCCATGCTTCCGTTTTGCGGGTATCACATGGGAGACTACTTTAATCACTGG
CTGCATATCGGCAGAACGGTGGTTGATCCTCCGAGAATTTTCGGCGTCAACTGGTTCCGCAAGGATGATGACGGGAAATT
TCTCTGGCCGGGGTTCGGTGAAAACATGCGTGTGCTGAAATGGATCGTTGATCGCGTTCGCGGCAGAGCCGGCGCAGTCG
AGAGCCCTCTTGGATGGATGCCGAGATATGAGATGCTTGGTTTTGAGGGAGATGACGGGTTAACGCAGGATCAGTTCACC
AAACTGATGTCTATAGATCGCGAGGCGTGGAAAAAAGAGCTGTTTTCTCACGAGGAGCTGCTTGAAAAACTCTATGACCG
TCTTCCAAAAGAGTTCAGTCATATCCGCGAGCTGATGCTTTCAACGCTCTGGATGTCTCCCGAGCACTGGGAGCTTGCTG
CCGAGCGTTACTCTGAAGATCAATAA

Upstream 100 bases:

>100_bases
AGAGCAGGGTTTGTGGGAAAATTTATCGTGTTAGTTGTACGTCCGAGGTCAGTGTTGGCTTCGGGCGTTTTTTATAATGA
GTAAAAAATAATTGTTAAAT

Downstream 100 bases:

>100_bases
CAATGCATGCTGGTTTTTAAGACGGTTGCCGGATAATACCTTGTAACGGTTAAACAAAATTATAATTACGTATGAAAATA
AACGCAGGATTACTCTTACT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTLVDINAPDFVVNKKLLQWVQETADLCRPDLVHWCDGSQEEYDLLCEQMVESGTFIRLSAEKRPNSFLCRSDPSDVARV
EDRTYICSIRKQDAGPTNNWVAPKEMKKILAGLYDGCMQGRTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRKVLDVLGANGDFVHCLHSVGAPLKAGDQDVSWPCSETKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASAMARD
EGWLAEHMLILGVESPDGEKTYVSAAFPSACGKTNFAMLIPPESFAGWKVTTIGDDIAWIKPGEDGRLHAINPEYGFFGV
APGTSDKSNPNAMETLKKNCIFTNVALTPDGDVWWEGMTDDIPEELTDWRGNPWTPGCGRPSSHPNARFTAPASQCPSID
PDWENPHGVPISAFIFGGRRRDTIPLVYQSANWYFGVYMAATMGSEMTAAAAGLIGDVRRDPYAMLPFCGYHMGDYFNHW
LHIGRTVVDPPRIFGVNWFRKDDDGKFLWPGFGENMRVLKWIVDRVRGRAGAVESPLGWMPRYEMLGFEGDDGLTQDQFT
KLMSIDREAWKKELFSHEELLEKLYDRLPKEFSHIRELMLSTLWMSPEHWELAAERYSEDQ

Sequences:

>Translated_621_residues
MTLVDINAPDFVVNKKLLQWVQETADLCRPDLVHWCDGSQEEYDLLCEQMVESGTFIRLSAEKRPNSFLCRSDPSDVARV
EDRTYICSIRKQDAGPTNNWVAPKEMKKILAGLYDGCMQGRTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRKVLDVLGANGDFVHCLHSVGAPLKAGDQDVSWPCSETKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASAMARD
EGWLAEHMLILGVESPDGEKTYVSAAFPSACGKTNFAMLIPPESFAGWKVTTIGDDIAWIKPGEDGRLHAINPEYGFFGV
APGTSDKSNPNAMETLKKNCIFTNVALTPDGDVWWEGMTDDIPEELTDWRGNPWTPGCGRPSSHPNARFTAPASQCPSID
PDWENPHGVPISAFIFGGRRRDTIPLVYQSANWYFGVYMAATMGSEMTAAAAGLIGDVRRDPYAMLPFCGYHMGDYFNHW
LHIGRTVVDPPRIFGVNWFRKDDDGKFLWPGFGENMRVLKWIVDRVRGRAGAVESPLGWMPRYEMLGFEGDDGLTQDQFT
KLMSIDREAWKKELFSHEELLEKLYDRLPKEFSHIRELMLSTLWMSPEHWELAAERYSEDQ
>Mature_620_residues
TLVDINAPDFVVNKKLLQWVQETADLCRPDLVHWCDGSQEEYDLLCEQMVESGTFIRLSAEKRPNSFLCRSDPSDVARVE
DRTYICSIRKQDAGPTNNWVAPKEMKKILAGLYDGCMQGRTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMG
RKVLDVLGANGDFVHCLHSVGAPLKAGDQDVSWPCSETKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASAMARDE
GWLAEHMLILGVESPDGEKTYVSAAFPSACGKTNFAMLIPPESFAGWKVTTIGDDIAWIKPGEDGRLHAINPEYGFFGVA
PGTSDKSNPNAMETLKKNCIFTNVALTPDGDVWWEGMTDDIPEELTDWRGNPWTPGCGRPSSHPNARFTAPASQCPSIDP
DWENPHGVPISAFIFGGRRRDTIPLVYQSANWYFGVYMAATMGSEMTAAAAGLIGDVRRDPYAMLPFCGYHMGDYFNHWL
HIGRTVVDPPRIFGVNWFRKDDDGKFLWPGFGENMRVLKWIVDRVRGRAGAVESPLGWMPRYEMLGFEGDDGLTQDQFTK
LMSIDREAWKKELFSHEELLEKLYDRLPKEFSHIRELMLSTLWMSPEHWELAAERYSEDQ

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=595, Percent_Identity=51.4285714285714, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI66346721, Length=587, Percent_Identity=52.2998296422487, Blast_Score=600, Evalue=1e-171,
Organism=Homo sapiens, GI66346723, Length=374, Percent_Identity=54.8128342245989, Blast_Score=404, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71989645, Length=591, Percent_Identity=49.5769881556684, Blast_Score=573, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17555492, Length=599, Percent_Identity=49.0818030050083, Blast_Score=572, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI71989658, Length=591, Percent_Identity=49.5769881556684, Blast_Score=572, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI71989653, Length=578, Percent_Identity=50, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI32564411, Length=552, Percent_Identity=51.0869565217391, Blast_Score=556, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564407, Length=500, Percent_Identity=51.6, Blast_Score=513, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71989649, Length=493, Percent_Identity=51.7241379310345, Blast_Score=502, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI32564409, Length=346, Percent_Identity=49.1329479768786, Blast_Score=340, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17540956, Length=490, Percent_Identity=25.7142857142857, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24655085, Length=567, Percent_Identity=52.2045855379189, Blast_Score=594, Evalue=1e-170,
Organism=Drosophila melanogaster, GI24655082, Length=603, Percent_Identity=50.2487562189055, Blast_Score=583, Evalue=1e-166,
Organism=Drosophila melanogaster, GI161077226, Length=565, Percent_Identity=51.3274336283186, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24655088, Length=464, Percent_Identity=55.1724137931034, Blast_Score=517, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 69823; Mature: 69692

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVDINAPDFVVNKKLLQWVQETADLCRPDLVHWCDGSQEEYDLLCEQMVESGTFIRLS
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEE
AEKRPNSFLCRSDPSDVARVEDRTYICSIRKQDAGPTNNWVAPKEMKKILAGLYDGCMQG
CCCCCCCEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMGRKVLDVLGANGDFVHCLHS
CEEEEEEECCCCCHHHHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VGAPLKAGDQDVSWPCSETKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASAMARD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
EGWLAEHMLILGVESPDGEKTYVSAAFPSACGKTNFAMLIPPESFAGWKVTTIGDDIAWI
CCHHEEEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEE
KPGEDGRLHAINPEYGFFGVAPGTSDKSNPNAMETLKKNCIFTNVALTPDGDVWWEGMTD
CCCCCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEECCCCC
DIPEELTDWRGNPWTPGCGRPSSHPNARFTAPASQCPSIDPDWENPHGVPISAFIFGGRR
CCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCEEEEEEECCCC
RDTIPLVYQSANWYFGVYMAATMGSEMTAAAAGLIGDVRRDPYAMLPFCGYHMGDYFNHW
CCCCEEEEECCCCEEEEEEEHHCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHH
LHIGRTVVDPPRIFGVNWFRKDDDGKFLWPGFGENMRVLKWIVDRVRGRAGAVESPLGWM
HHHCCCCCCCCCEECCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PRYEMLGFEGDDGLTQDQFTKLMSIDREAWKKELFSHEELLEKLYDRLPKEFSHIRELML
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLWMSPEHWELAAERYSEDQ
HHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TLVDINAPDFVVNKKLLQWVQETADLCRPDLVHWCDGSQEEYDLLCEQMVESGTFIRLS
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEE
AEKRPNSFLCRSDPSDVARVEDRTYICSIRKQDAGPTNNWVAPKEMKKILAGLYDGCMQG
CCCCCCCEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMGRKVLDVLGANGDFVHCLHS
CEEEEEEECCCCCHHHHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VGAPLKAGDQDVSWPCSETKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASAMARD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
EGWLAEHMLILGVESPDGEKTYVSAAFPSACGKTNFAMLIPPESFAGWKVTTIGDDIAWI
CCHHEEEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEE
KPGEDGRLHAINPEYGFFGVAPGTSDKSNPNAMETLKKNCIFTNVALTPDGDVWWEGMTD
CCCCCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEECCCCC
DIPEELTDWRGNPWTPGCGRPSSHPNARFTAPASQCPSIDPDWENPHGVPISAFIFGGRR
CCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCEEEEEEECCCC
RDTIPLVYQSANWYFGVYMAATMGSEMTAAAAGLIGDVRRDPYAMLPFCGYHMGDYFNHW
CCCCEEEEECCCCEEEEEEEHHCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHH
LHIGRTVVDPPRIFGVNWFRKDDDGKFLWPGFGENMRVLKWIVDRVRGRAGAVESPLGWM
HHHCCCCCCCCCEECCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PRYEMLGFEGDDGLTQDQFTKLMSIDREAWKKELFSHEELLEKLYDRLPKEFSHIRELML
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLWMSPEHWELAAERYSEDQ
HHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]