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The map label for this gene is pckA

Identifier: 118582002

GI number: 118582002

Start: 3985228

End: 3986820

Strand: Direct

Name: pckA

Synonym: Ppro_3603

Alternate gene names: 118582002

Gene position: 3985228-3986820 (Clockwise)

Preceding gene: 118582001

Following gene: NA

Centisome position: 99.43

GC content: 62.4

Gene sequence:

>1593_bases
GTGAAATTCAACGATGTTACCCGTGGAACCGGTCTTGAAGAGCATGGCATCACCAACGCGAACCTGATCTACTGGACCCC
GCCGACCTCAGTGCTCTACGAGCAGATCATCAAACGGGGTGAGGGGCTGGTTTCGCACATGGGCGCCCTGGCCGTGAAGA
CCGGCCACTACACCGGACGGGCCGCCAACGAGAAATTCATCGTCGATGAACCCACCTGCCATGAAAACGTCGCCTGGGGC
AAGGTCAACAAACCGTTCGACCCGCAGAAGTTCGACGAGCTGTACAAGCGCATGCTGGCCTACATGGATGGCCGCAACCT
GTTCGTGCAGGACTGCTTCGCCGGCGCCGACAGAGAACACCGCCTGCCCCTGCGCATCATCACCGAGAGGGCCTGGCACT
CGCTCTTTGCCCGCAACATGTTCATCCGGGCCACACCGGAGGAGCTGGAACAGCACGAACCGCGCTTCGCCCTGATCAAC
CTGCCAGGCTTCCACGCCATTCCCTCCATTGACGGCACCCATTCCGAGGCCTTCATCATTGTCAACCTGGGCAGGAAGCT
GATCCTGATCGGCGGCACCAGCTATGCCGGCGAGATCAAGAAATCCATCTTCACCATCCTCAACTACATCCTGCCGGTGG
AGAAGAAGATCCTCTCCATGCACTGCTCGGCCAACGTGGGACCGAAGGGCGATTCTGCGGTGTTCTTCGGCCTCTCCGGG
ACCGGCAAGACCACCCTGTCGGCCGATTCCAGCCGGGCGCTGATCGGCGATGACGAGCACGGCTGGGACGACAAGGGGCT
GTTCAACTTCGAAGGAGGCTGCTACGCCAAGATCATCCGCCTCTGCCCGGAGAGCGAGCCGGAAATCTTTGCCACGACCC
GCAGGTTCGGGACGATCCTGGAAAACGTGGCCATCAACACCCGTACCCGCCGGGTGGACCTGGACGACGACTCCTTCACC
GAGAACACCCGCGCCTCCTACCCCCTGACCCACATCCCCAACATCGTTCCCTCGGGGATTGCCGGTCATCCCGCCAACAT
CATCATGCTGACCTGCGACGCCTACGGCGTGCTCCCCCCCATCTCCCACCTGACCAAGGAGCAGGCCATGTACCACTTCC
TGTCCGGCTACACCGCCCGTGTGGCCGGAACCGAGGCCGGTGTCAAGGAACCCACCGCCACCTTCTCCACCTGCTTCGGC
GGCCCCTTCATGGCACTCAATCCGACGGTGTACGGAGAACTGCTGCGGGAAAAGATCTCCCGCCACAACGTCTCCTGCTG
GCTGGTCAATACCGGCTGGAACGGCGGCCCCTACGGAGTGGGCGAACGCATCAGGATCAGCTACTCCCGCGCCCTGATCA
ATGCGGCCCTGGACGGGACCCTGGCCGACGGCAGCTTCGAAACCGACCCTTTCTTCGGGCTGGCCATCCCCACCAGCTGT
CCCGGGGTTCCCTCGGAAATGCTCAACCCGCGCAACACCTGGTCCGATCCGGCCAGGTACGACGATACCGCCTCCAGGCT
GGTGGCCATGTTCAGGTCCAACTTCACCAAGTACCAGCCCTATGTCAGCGCCGAGGTGGCCAACGCGCTGTAG

Upstream 100 bases:

>100_bases
TGGAGCCGGGAAAAAGCGGGTGAGGAAGCCGGGGAGATGTGTAGGATGGATTTTATCGAATAATTACCTCTGAATAATGA
GGGACAGGGGAGGAACGGTA

Downstream 100 bases:

>100_bases
CTGCTTGTAAGGCACAAAAAAGGGGGGGCGCCAGCAGATGTAGCGTCCCCCCTGTTTTTGTCAGCCAACATAATCGGGTA
GGAGGCGGGATTACTCCCGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKFNDVTRGTGLEEHGITNANLIYWTPPTSVLYEQIIKRGEGLVSHMGALAVKTGHYTGRAANEKFIVDEPTCHENVAWG
KVNKPFDPQKFDELYKRMLAYMDGRNLFVQDCFAGADREHRLPLRIITERAWHSLFARNMFIRATPEELEQHEPRFALIN
LPGFHAIPSIDGTHSEAFIIVNLGRKLILIGGTSYAGEIKKSIFTILNYILPVEKKILSMHCSANVGPKGDSAVFFGLSG
TGKTTLSADSSRALIGDDEHGWDDKGLFNFEGGCYAKIIRLCPESEPEIFATTRRFGTILENVAINTRTRRVDLDDDSFT
ENTRASYPLTHIPNIVPSGIAGHPANIIMLTCDAYGVLPPISHLTKEQAMYHFLSGYTARVAGTEAGVKEPTATFSTCFG
GPFMALNPTVYGELLREKISRHNVSCWLVNTGWNGGPYGVGERIRISYSRALINAALDGTLADGSFETDPFFGLAIPTSC
PGVPSEMLNPRNTWSDPARYDDTASRLVAMFRSNFTKYQPYVSAEVANAL

Sequences:

>Translated_530_residues
MKFNDVTRGTGLEEHGITNANLIYWTPPTSVLYEQIIKRGEGLVSHMGALAVKTGHYTGRAANEKFIVDEPTCHENVAWG
KVNKPFDPQKFDELYKRMLAYMDGRNLFVQDCFAGADREHRLPLRIITERAWHSLFARNMFIRATPEELEQHEPRFALIN
LPGFHAIPSIDGTHSEAFIIVNLGRKLILIGGTSYAGEIKKSIFTILNYILPVEKKILSMHCSANVGPKGDSAVFFGLSG
TGKTTLSADSSRALIGDDEHGWDDKGLFNFEGGCYAKIIRLCPESEPEIFATTRRFGTILENVAINTRTRRVDLDDDSFT
ENTRASYPLTHIPNIVPSGIAGHPANIIMLTCDAYGVLPPISHLTKEQAMYHFLSGYTARVAGTEAGVKEPTATFSTCFG
GPFMALNPTVYGELLREKISRHNVSCWLVNTGWNGGPYGVGERIRISYSRALINAALDGTLADGSFETDPFFGLAIPTSC
PGVPSEMLNPRNTWSDPARYDDTASRLVAMFRSNFTKYQPYVSAEVANAL
>Mature_530_residues
MKFNDVTRGTGLEEHGITNANLIYWTPPTSVLYEQIIKRGEGLVSHMGALAVKTGHYTGRAANEKFIVDEPTCHENVAWG
KVNKPFDPQKFDELYKRMLAYMDGRNLFVQDCFAGADREHRLPLRIITERAWHSLFARNMFIRATPEELEQHEPRFALIN
LPGFHAIPSIDGTHSEAFIIVNLGRKLILIGGTSYAGEIKKSIFTILNYILPVEKKILSMHCSANVGPKGDSAVFFGLSG
TGKTTLSADSSRALIGDDEHGWDDKGLFNFEGGCYAKIIRLCPESEPEIFATTRRFGTILENVAINTRTRRVDLDDDSFT
ENTRASYPLTHIPNIVPSGIAGHPANIIMLTCDAYGVLPPISHLTKEQAMYHFLSGYTARVAGTEAGVKEPTATFSTCFG
GPFMALNPTVYGELLREKISRHNVSCWLVNTGWNGGPYGVGERIRISYSRALINAALDGTLADGSFETDPFFGLAIPTSC
PGVPSEMLNPRNTWSDPARYDDTASRLVAMFRSNFTKYQPYVSAEVANAL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=531, Percent_Identity=47.4576271186441, Blast_Score=484, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322950, Length=508, Percent_Identity=46.8503937007874, Blast_Score=468, Evalue=1e-132,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_PELPD (A1AV24)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_903252.1
- ProteinModelPortal:   A1AV24
- SMR:   A1AV24
- STRING:   A1AV24
- GeneID:   4574531
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3603
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   A1AV24
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58589; Mature: 58589

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNDVTRGTGLEEHGITNANLIYWTPPTSVLYEQIIKRGEGLVSHMGALAVKTGHYTGR
CCCCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCC
AANEKFIVDEPTCHENVAWGKVNKPFDPQKFDELYKRMLAYMDGRNLFVQDCFAGADREH
CCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCC
RLPLRIITERAWHSLFARNMFIRATPEELEQHEPRFALINLPGFHAIPSIDGTHSEAFII
CCCHHHHHHHHHHHHHHHCEEEEECHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEE
VNLGRKLILIGGTSYAGEIKKSIFTILNYILPVEKKILSMHCSANVGPKGDSAVFFGLSG
EECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
TGKTTLSADSSRALIGDDEHGWDDKGLFNFEGGCYAKIIRLCPESEPEIFATTRRFGTIL
CCCEEECCCCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCEEEHHHHHHHHH
ENVAINTRTRRVDLDDDSFTENTRASYPLTHIPNIVPSGIAGHPANIIMLTCDAYGVLPP
HHHEECCEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCC
ISHLTKEQAMYHFLSGYTARVAGTEAGVKEPTATFSTCFGGPFMALNPTVYGELLREKIS
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEECHHHHHHHHHHHHH
RHNVSCWLVNTGWNGGPYGVGERIRISYSRALINAALDGTLADGSFETDPFFGLAIPTSC
HCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHCCEECCCCCCCCCEEEEECCCCC
PGVPSEMLNPRNTWSDPARYDDTASRLVAMFRSNFTKYQPYVSAEVANAL
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKFNDVTRGTGLEEHGITNANLIYWTPPTSVLYEQIIKRGEGLVSHMGALAVKTGHYTGR
CCCCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCC
AANEKFIVDEPTCHENVAWGKVNKPFDPQKFDELYKRMLAYMDGRNLFVQDCFAGADREH
CCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCC
RLPLRIITERAWHSLFARNMFIRATPEELEQHEPRFALINLPGFHAIPSIDGTHSEAFII
CCCHHHHHHHHHHHHHHHCEEEEECHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEE
VNLGRKLILIGGTSYAGEIKKSIFTILNYILPVEKKILSMHCSANVGPKGDSAVFFGLSG
EECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
TGKTTLSADSSRALIGDDEHGWDDKGLFNFEGGCYAKIIRLCPESEPEIFATTRRFGTIL
CCCEEECCCCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCEEEHHHHHHHHH
ENVAINTRTRRVDLDDDSFTENTRASYPLTHIPNIVPSGIAGHPANIIMLTCDAYGVLPP
HHHEECCEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCC
ISHLTKEQAMYHFLSGYTARVAGTEAGVKEPTATFSTCFGGPFMALNPTVYGELLREKIS
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEECHHHHHHHHHHHHH
RHNVSCWLVNTGWNGGPYGVGERIRISYSRALINAALDGTLADGSFETDPFFGLAIPTSC
HCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHCCEECCCCCCCCCEEEEECCCCC
PGVPSEMLNPRNTWSDPARYDDTASRLVAMFRSNFTKYQPYVSAEVANAL
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA