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The map label for this gene is ytcB [H]

Identifier: 118581970

GI number: 118581970

Start: 3947268

End: 3948233

Strand: Reverse

Name: ytcB [H]

Synonym: Ppro_3571

Alternate gene names: 118581970

Gene position: 3948233-3947268 (Counterclockwise)

Preceding gene: 118581971

Following gene: 118581969

Centisome position: 98.51

GC content: 57.25

Gene sequence:

>966_bases
ATGAGAAAAGTGCTTGTCACCGGGGCCGGTGGTTTTATCGGCCACCATTTGGTAAAGGATCTGGTACGTCGGGGCCACGA
AGTGATTGCTGTGGACCGAAAACTCCCCGAGTTCGAAAAGAGTGCTGCGAGCCGGTTCGTGTTGCAGGATCTTCGCGAAA
CAACGGCTGAATGGGAATCGCTCTTCCTGGGAGTAGACGATGTGTATGCGCTTGCCGCAGACATGGGCGGGATGGGTTTC
ATCTCCCGCAATCACGCCGATATCATGCGGGACAACACCCGTATTGACATCAACACCCTTGAGGCCGCCCGCAAGGCGAA
GGTAGGGCGCCTGCTCTACACATCATCAGCCTGCGTTTATCCTGAACACCTCCAGGAGGCGGAAGCGGCGATACCCCTGG
CAGAGACAATGGCGTATCCTGCCAAGCCCCAGGACGGGTATGGCTGGGAAAAACTCTATGCCGAGCAACTGTGCCATTAT
TACCGCCTCGAACACGGGGTTGATACCCGTATTGTCCGTTTTCACAATATCTACGGCCCACTGGGAGCCTGGCAGGGAGG
GAGGGAGAAGGCTCCGGCTGCCCTGTGCCGAAAAGTAGCCGAGGCATGTCTCAAGGGCAGGGATAGTATCCAGATCTGGG
GTGATGGTAGACAGACCCGCTCATTCTGCTTTATCGATGACTGCATCCAGGGCTTGGCGCGCATCCTGGAATCCGGCTAC
ACCGAGCCACTGAACCTTGGTCGCGACGAGATGGTTTCGATCAACGAACTTGCCCGGCTGATTTTCGAGGTCGCGGGCGC
CGAACTCCGCATCGAACATATTGAGGGACCACAGGGAGTGCGTGGGCGCAACTCCGACAACAAACGTCTTGCCGAGGTTA
CCGGGTTTACCCCAAGTATCAGCCTTCGTCAGGGGATAGCCGCGACCTATGGCTGGATCGAGGCACAGGTGAGAGCCGGA
ATATGA

Upstream 100 bases:

>100_bases
ACCTGTCGAATTCTGATGCATTGGTGTCAATTTCTGGCTACTTTGAGTATATTGCGGTTCTCTTCTGGTCGTTTCGAGAG
GCATGGGGGGAGTTCTTGTT

Downstream 100 bases:

>100_bases
GCTTCTCATCTGAATCTTCTCCGCAGAAGCGAACCCTCCTGTTTGTGATGGCGTACCATAACGAACGAGAGTTCGCTATG
TTTCAGGAACTGCTCTCCCG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRKVLVTGAGGFIGHHLVKDLVRRGHEVIAVDRKLPEFEKSAASRFVLQDLRETTAEWESLFLGVDDVYALAADMGGMGF
ISRNHADIMRDNTRIDINTLEAARKAKVGRLLYTSSACVYPEHLQEAEAAIPLAETMAYPAKPQDGYGWEKLYAEQLCHY
YRLEHGVDTRIVRFHNIYGPLGAWQGGREKAPAALCRKVAEACLKGRDSIQIWGDGRQTRSFCFIDDCIQGLARILESGY
TEPLNLGRDEMVSINELARLIFEVAGAELRIEHIEGPQGVRGRNSDNKRLAEVTGFTPSISLRQGIAATYGWIEAQVRAG
I

Sequences:

>Translated_321_residues
MRKVLVTGAGGFIGHHLVKDLVRRGHEVIAVDRKLPEFEKSAASRFVLQDLRETTAEWESLFLGVDDVYALAADMGGMGF
ISRNHADIMRDNTRIDINTLEAARKAKVGRLLYTSSACVYPEHLQEAEAAIPLAETMAYPAKPQDGYGWEKLYAEQLCHY
YRLEHGVDTRIVRFHNIYGPLGAWQGGREKAPAALCRKVAEACLKGRDSIQIWGDGRQTRSFCFIDDCIQGLARILESGY
TEPLNLGRDEMVSINELARLIFEVAGAELRIEHIEGPQGVRGRNSDNKRLAEVTGFTPSISLRQGIAATYGWIEAQVRAG
I
>Mature_321_residues
MRKVLVTGAGGFIGHHLVKDLVRRGHEVIAVDRKLPEFEKSAASRFVLQDLRETTAEWESLFLGVDDVYALAADMGGMGF
ISRNHADIMRDNTRIDINTLEAARKAKVGRLLYTSSACVYPEHLQEAEAAIPLAETMAYPAKPQDGYGWEKLYAEQLCHY
YRLEHGVDTRIVRFHNIYGPLGAWQGGREKAPAALCRKVAEACLKGRDSIQIWGDGRQTRSFCFIDDCIQGLARILESGY
TEPLNLGRDEMVSINELARLIFEVAGAELRIEHIEGPQGVRGRNSDNKRLAEVTGFTPSISLRQGIAATYGWIEAQVRAG
I

Specific function: Two Step Nadp-Dependent Conversion Of Gdp-4-Dehydro-6- Deoxy-D-Mannose To Gdp-Fucose, Involving An Epimerase And A Reductase Reaction. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=309, Percent_Identity=27.831715210356, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4507709, Length=325, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788365, Length=329, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786974, Length=267, Percent_Identity=26.9662921348315, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=28.8288288288288, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.969696969697, Blast_Score=121, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 35732; Mature: 35732

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLVTGAGGFIGHHLVKDLVRRGHEVIAVDRKLPEFEKSAASRFVLQDLRETTAEWES
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFLGVDDVYALAADMGGMGFISRNHADIMRDNTRIDINTLEAARKAKVGRLLYTSSACVY
HHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHEEEECCCCCC
PEHLQEAEAAIPLAETMAYPAKPQDGYGWEKLYAEQLCHYYRLEHGVDTRIVRFHNIYGP
HHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCC
LGAWQGGREKAPAALCRKVAEACLKGRDSIQIWGDGRQTRSFCFIDDCIQGLARILESGY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHCCC
TEPLNLGRDEMVSINELARLIFEVAGAELRIEHIEGPQGVRGRNSDNKRLAEVTGFTPSI
CCCCCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCH
SLRQGIAATYGWIEAQVRAGI
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKVLVTGAGGFIGHHLVKDLVRRGHEVIAVDRKLPEFEKSAASRFVLQDLRETTAEWES
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFLGVDDVYALAADMGGMGFISRNHADIMRDNTRIDINTLEAARKAKVGRLLYTSSACVY
HHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHEEEECCCCCC
PEHLQEAEAAIPLAETMAYPAKPQDGYGWEKLYAEQLCHYYRLEHGVDTRIVRFHNIYGP
HHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCC
LGAWQGGREKAPAALCRKVAEACLKGRDSIQIWGDGRQTRSFCFIDDCIQGLARILESGY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHCCC
TEPLNLGRDEMVSINELARLIFEVAGAELRIEHIEGPQGVRGRNSDNKRLAEVTGFTPSI
CCCCCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCH
SLRQGIAATYGWIEAQVRAGI
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]