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The map label for this gene is prfA [H]

Identifier: 118581948

GI number: 118581948

Start: 3920779

End: 3921108

Strand: Direct

Name: prfA [H]

Synonym: Ppro_3548

Alternate gene names: 118581948

Gene position: 3920779-3921108 (Clockwise)

Preceding gene: 118581947

Following gene: 118581949

Centisome position: 97.82

GC content: 66.06

Gene sequence:

>330_bases
ATGACGACCATGGTCGAGATCAGGGAAGAGGATATCAGGATCGAATACTACCGTGCTTCCGGGCCGGGCGGCCAGCACCG
CAACACCACCGACTCGGCGGTGCGCATCCGCCACCTCCCCACCGGCATCGTGGCCCAGGCGTCCGAGAGCCGCTCCCAGT
CGCGAAACCGCGAGAGGGCCATGGAACGGCTGACCGCGGCACTGGAAAAGCGTGAACGGGTTGTGAAGAAGCGCATCGCC
ACCAGGGTGCCCCGCGGCGCCCGGGAGAAGCGCCTGGGCGACAAGAAGATCGTCTCCCTGCGCAAGAGGGAACGCTCCAC
GTTGGACTGA

Upstream 100 bases:

>100_bases
CGGCTGCCGGATGTGCAGGAGAACCGATCATGAAGCGGCGGCTTTTCAACCCGGATTCGGCGGTTGAGAGCTGCCGGAGT
TCGTGAGGACAGGGGAAAGG

Downstream 100 bases:

>100_bases
ATGGATGCAAAAAGACGTTGGAATAGCACCGCGGGCTTGCTATTCTGCTCTGCCAGCCGCAGGCTGTGCGTGTTTTTTTC
TGAAAGGAACTCCGTTGCAA

Product: class I peptide chain release factor

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MTTMVEIREEDIRIEYYRASGPGGQHRNTTDSAVRIRHLPTGIVAQASESRSQSRNRERAMERLTAALEKRERVVKKRIA
TRVPRGAREKRLGDKKIVSLRKRERSTLD

Sequences:

>Translated_109_residues
MTTMVEIREEDIRIEYYRASGPGGQHRNTTDSAVRIRHLPTGIVAQASESRSQSRNRERAMERLTAALEKRERVVKKRIA
TRVPRGAREKRLGDKKIVSLRKRERSTLD
>Mature_108_residues
TTMVEIREEDIRIEYYRASGPGGQHRNTTDSAVRIRHLPTGIVAQASESRSQSRNRERAMERLTAALEKRERVVKKRIAT
RVPRGAREKRLGDKKIVSLRKRERSTLD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=62, Percent_Identity=59.6774193548387, Blast_Score=78, Evalue=1e-15,
Organism=Homo sapiens, GI34577120, Length=67, Percent_Identity=47.7611940298507, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI2367172, Length=68, Percent_Identity=52.9411764705882, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1787462, Length=63, Percent_Identity=52.3809523809524, Blast_Score=76, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321295, Length=58, Percent_Identity=53.448275862069, Blast_Score=67, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921226, Length=80, Percent_Identity=55, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 12627; Mature: 12495

Theoretical pI: Translated: 11.82; Mature: 11.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMVEIREEDIRIEYYRASGPGGQHRNTTDSAVRIRHLPTGIVAQASESRSQSRNRERA
CCCHHEECCCCEEEEEEECCCCCCCCCCCCCHHEEHHCCCCCHHHHHHHHHHHHHHHHHH
MERLTAALEKRERVVKKRIATRVPRGAREKRLGDKKIVSLRKRERSTLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTMVEIREEDIRIEYYRASGPGGQHRNTTDSAVRIRHLPTGIVAQASESRSQSRNRERA
CCHHEECCCCEEEEEEECCCCCCCCCCCCCHHEEHHCCCCCHHHHHHHHHHHHHHHHHH
MERLTAALEKRERVVKKRIATRVPRGAREKRLGDKKIVSLRKRERSTLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA