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The map label for this gene is def [H]

Identifier: 118581913

GI number: 118581913

Start: 3884048

End: 3884554

Strand: Reverse

Name: def [H]

Synonym: Ppro_3513

Alternate gene names: 118581913

Gene position: 3884554-3884048 (Counterclockwise)

Preceding gene: 118581914

Following gene: 118581912

Centisome position: 96.92

GC content: 62.72

Gene sequence:

>507_bases
ATGGCCATTCATCCCATTCTGCGGTATCCCCATCCGCTGCTGAAAAAAATCTGCCACGAGGTTACCGGGATCGACCGGGA
GATCCATGCCCTGATTCAGGATCTGCTGGACACCATGCGGGCCGGTCCCGGATCGGTGGGGGTGGCGGCTCCCCAGATCG
GCGTCACCCTGCGGGCCTGCGTCGTTGACGTCTCGAACAGCCGTAACGGAAGGGAGAACAATCACGGCCTGCTGCGTATG
ATCAACCCGCGCATCATCGCCCGCTCGGGTTCTGCGGTCATGCGCGAGGGGTGCATGAGCGTGCCGGACTACACCGGCGA
TGTGGAGCGCTCCACCGAGCTGAGGGTAAGCTTCCTGGAGCCGGAGGGGGAGCTGCGCGAGATAACCGCCAGTGGCTTCG
AGGCGGTCGCCCTGCAGCACGAGATGGATCACCTGGACGGCATACTGTTTCTGGACCGGATCGTCAGCGTCAAGACCGGT
CTGTTCCGGAGGAAAACCTACGCATAG

Upstream 100 bases:

>100_bases
CCAGACCCCGCATCAGCTGGAAAAGCTGCTGGAAAACCTGAAGAAGGAGCTTGATGTGGAGATCGGTGTGCGTATCGTCA
CCCCGGTTTCCCTCTGATCC

Downstream 100 bases:

>100_bases
GAGATCGCCATGCCTGAACTGCTGACACCCGACGAGCAGACGGTACTGCTCAGGATAGCCCGCGAGACCATTGTCGCCCA
CGTCACCGGCAGCACTATCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MAIHPILRYPHPLLKKICHEVTGIDREIHALIQDLLDTMRAGPGSVGVAAPQIGVTLRACVVDVSNSRNGRENNHGLLRM
INPRIIARSGSAVMREGCMSVPDYTGDVERSTELRVSFLEPEGELREITASGFEAVALQHEMDHLDGILFLDRIVSVKTG
LFRRKTYA

Sequences:

>Translated_168_residues
MAIHPILRYPHPLLKKICHEVTGIDREIHALIQDLLDTMRAGPGSVGVAAPQIGVTLRACVVDVSNSRNGRENNHGLLRM
INPRIIARSGSAVMREGCMSVPDYTGDVERSTELRVSFLEPEGELREITASGFEAVALQHEMDHLDGILFLDRIVSVKTG
LFRRKTYA
>Mature_167_residues
AIHPILRYPHPLLKKICHEVTGIDREIHALIQDLLDTMRAGPGSVGVAAPQIGVTLRACVVDVSNSRNGRENNHGLLRMI
NPRIIARSGSAVMREGCMSVPDYTGDVERSTELRVSFLEPEGELREITASGFEAVALQHEMDHLDGILFLDRIVSVKTGL
FRRKTYA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=141, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1789682, Length=166, Percent_Identity=34.3373493975904, Blast_Score=108, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=33.1210191082803, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24645726, Length=142, Percent_Identity=34.5070422535211, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18596; Mature: 18465

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHPILRYPHPLLKKICHEVTGIDREIHALIQDLLDTMRAGPGSVGVAAPQIGVTLRAC
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VVDVSNSRNGRENNHGLLRMINPRIIARSGSAVMREGCMSVPDYTGDVERSTELRVSFLE
EEECCCCCCCCCCCCCEEEECCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEEC
PEGELREITASGFEAVALQHEMDHLDGILFLDRIVSVKTGLFRRKTYA
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIHPILRYPHPLLKKICHEVTGIDREIHALIQDLLDTMRAGPGSVGVAAPQIGVTLRAC
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VVDVSNSRNGRENNHGLLRMINPRIIARSGSAVMREGCMSVPDYTGDVERSTELRVSFLE
EEECCCCCCCCCCCCCEEEECCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEEC
PEGELREITASGFEAVALQHEMDHLDGILFLDRIVSVKTGLFRRKTYA
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]