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The map label for this gene is nifK [H]

Identifier: 118581869

GI number: 118581869

Start: 3832461

End: 3833924

Strand: Direct

Name: nifK [H]

Synonym: Ppro_3469

Alternate gene names: 118581869

Gene position: 3832461-3833924 (Clockwise)

Preceding gene: 118581868

Following gene: 118581874

Centisome position: 95.62

GC content: 59.43

Gene sequence:

>1464_bases
ATGAGCGCAGATGCCGCCGTCAAATATGTTACCGAAATAACCCCAGAGGATGAATCGCGGGTCGCGGAATGGATCAACAC
CGAGGAGTACAAGGAGAAGAACTTCGCCCGCCAGGCCCTGGTGATCAACCCGGCCCACACCTGCCAGCCCCTGGGTGCCG
AACTGGTGGCCCATTCCTTCGAGGGAACCCTGCCGTTCGTCCATGGATCACAGGGGTGCGCATCCTACTACCGCAGCACC
CTGAACCGGCATTTCCGCGAACCAGCTCCCGCAGTGTCCGATGCCATGACCGAGGACGGCGCGGTGTTCGGCGGCCAGAA
CAACCTGCATGAGGCGCTGGAAAACGCCTATGCGCTGTACAAGCCGAAGATGATCGGGATCTTCACCTCCTGCATGCCGG
AGGTCATCGGCGACGACCTGACCGCCTTCATCAAAAATGCCCGTCAGAAGGGGTTCATTCCCCAGGACTTCCCCGTAGCC
TACGCCAACACGCCCAGTTTCAACGGCTCCCATATCCACGGCTACGACTCCATGCTGCTCTCCATGCTCCAGACCTTGAC
CGAGGGAAAACGGATGGAGGGGCGCTGCACCGGCAAGCTCAACCTGATCCCTGGCTGTGACTTCAACACCGGCAACTACC
GTGAATACAAGCGCATCCTGAAGGAGTTCGGCATCCCCGCTACCGTGCTTGCCGACATCTCCGATGTGTTCGACTCCCCC
CTTGACGGCACCTACCGTCTCTACCCGGGCGGCACCAAGCTGGAGGACGCGGCCGACTCCATCAACGGCAAGGTAACCAT
GACCCTGGGCAAATTCGCCACGACCAGGACCTTTAGCTGGATCAAGGACAACTACGCCGGAGAGCACCTGTCCTTGCCGA
CCCCCTTCGGCATCGAGAAGAGCGACGCCTTCATGATGAAGCTGGCCGAACTGTTCGGTAAACCGGTGCCCGAGTCGCTG
AAGGCCGAGCGGGGCAGGGCGGTGGACGCCATGACCGATGCCCACCAGTACATTCACAACAAGAAGTTCGCCGTCTACGG
CGACCCCGACTACCTGCTGGGCTATGTCTCCTTCCTGCTAGAAATGGGTGCACAGCCGTACCACATCCTCTGCAGCAAGG
GGAGCAAAAAACTGGAAAAGGATATCCAGGCCCTGCTGGACAGCTCGCTCTATGGCAAAGACGCAAAGATCTACATGAAC
AAGGACCTGTGGCACCTGCGCAGCCTGGTCATGACCGATCCTGTGGACGCCATCATCGGCGATACCCACGGCAAGTTCAT
CAGCCGCGACGCCAAGATCCCGCTGTTCCGCTTCGGTTTCCCGATCTTCGACCGGGTCAACCTGCACCGCTCTCCGCTGA
TCGGTTACCAGGGGGTGATCAACATGGTCACCACCATCTGCAACAAGTTCATCGACATCACCGATGACACCTGCGATGAC
CGGCATTTCGAGATGATGAGGTAG

Upstream 100 bases:

>100_bases
ACTCCCTGTTTTTACCGCCTCACGCCTCGCCCATCACCCCTCACCTTTCACATTTCCGACCGTGGAACGGATTGGTTCCC
GGCGTACAAAGGAGAAAACC

Downstream 100 bases:

>100_bases
ATGGAGCAAGGGGATACACGAAAATGACAAGGGGCGCTCCATCCGGGGCGCCCCTCTCTGTTCTCGTTTCCTATACGGCT
TGAATTCCGCGGCTGGAGCC

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSADAAVKYVTEITPEDESRVAEWINTEEYKEKNFARQALVINPAHTCQPLGAELVAHSFEGTLPFVHGSQGCASYYRST
LNRHFREPAPAVSDAMTEDGAVFGGQNNLHEALENAYALYKPKMIGIFTSCMPEVIGDDLTAFIKNARQKGFIPQDFPVA
YANTPSFNGSHIHGYDSMLLSMLQTLTEGKRMEGRCTGKLNLIPGCDFNTGNYREYKRILKEFGIPATVLADISDVFDSP
LDGTYRLYPGGTKLEDAADSINGKVTMTLGKFATTRTFSWIKDNYAGEHLSLPTPFGIEKSDAFMMKLAELFGKPVPESL
KAERGRAVDAMTDAHQYIHNKKFAVYGDPDYLLGYVSFLLEMGAQPYHILCSKGSKKLEKDIQALLDSSLYGKDAKIYMN
KDLWHLRSLVMTDPVDAIIGDTHGKFISRDAKIPLFRFGFPIFDRVNLHRSPLIGYQGVINMVTTICNKFIDITDDTCDD
RHFEMMR

Sequences:

>Translated_487_residues
MSADAAVKYVTEITPEDESRVAEWINTEEYKEKNFARQALVINPAHTCQPLGAELVAHSFEGTLPFVHGSQGCASYYRST
LNRHFREPAPAVSDAMTEDGAVFGGQNNLHEALENAYALYKPKMIGIFTSCMPEVIGDDLTAFIKNARQKGFIPQDFPVA
YANTPSFNGSHIHGYDSMLLSMLQTLTEGKRMEGRCTGKLNLIPGCDFNTGNYREYKRILKEFGIPATVLADISDVFDSP
LDGTYRLYPGGTKLEDAADSINGKVTMTLGKFATTRTFSWIKDNYAGEHLSLPTPFGIEKSDAFMMKLAELFGKPVPESL
KAERGRAVDAMTDAHQYIHNKKFAVYGDPDYLLGYVSFLLEMGAQPYHILCSKGSKKLEKDIQALLDSSLYGKDAKIYMN
KDLWHLRSLVMTDPVDAIIGDTHGKFISRDAKIPLFRFGFPIFDRVNLHRSPLIGYQGVINMVTTICNKFIDITDDTCDD
RHFEMMR
>Mature_486_residues
SADAAVKYVTEITPEDESRVAEWINTEEYKEKNFARQALVINPAHTCQPLGAELVAHSFEGTLPFVHGSQGCASYYRSTL
NRHFREPAPAVSDAMTEDGAVFGGQNNLHEALENAYALYKPKMIGIFTSCMPEVIGDDLTAFIKNARQKGFIPQDFPVAY
ANTPSFNGSHIHGYDSMLLSMLQTLTEGKRMEGRCTGKLNLIPGCDFNTGNYREYKRILKEFGIPATVLADISDVFDSPL
DGTYRLYPGGTKLEDAADSINGKVTMTLGKFATTRTFSWIKDNYAGEHLSLPTPFGIEKSDAFMMKLAELFGKPVPESLK
AERGRAVDAMTDAHQYIHNKKFAVYGDPDYLLGYVSFLLEMGAQPYHILCSKGSKKLEKDIQALLDSSLYGKDAKIYMNK
DLWHLRSLVMTDPVDAIIGDTHGKFISRDAKIPLFRFGFPIFDRVNLHRSPLIGYQGVINMVTTICNKFIDITDDTCDDR
HFEMMR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54460; Mature: 54328

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADAAVKYVTEITPEDESRVAEWINTEEYKEKNFARQALVINPAHTCQPLGAELVAHSF
CCCCHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHEEEECCHHHCCHHHHHHHHHHC
EGTLPFVHGSQGCASYYRSTLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEALENAYALY
CCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHHH
KPKMIGIFTSCMPEVIGDDLTAFIKNARQKGFIPQDFPVAYANTPSFNGSHIHGYDSMLL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEECCHHHHHH
SMLQTLTEGKRMEGRCTGKLNLIPGCDFNTGNYREYKRILKEFGIPATVLADISDVFDSP
HHHHHHHCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCC
LDGTYRLYPGGTKLEDAADSINGKVTMTLGKFATTRTFSWIKDNYAGEHLSLPTPFGIEK
CCCEEEECCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SDAFMMKLAELFGKPVPESLKAERGRAVDAMTDAHQYIHNKKFAVYGDPDYLLGYVSFLL
HHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH
EMGAQPYHILCSKGSKKLEKDIQALLDSSLYGKDAKIYMNKDLWHLRSLVMTDPVDAIIG
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHC
DTHGKFISRDAKIPLFRFGFPIFDRVNLHRSPLIGYQGVINMVTTICNKFIDITDDTCDD
CCCCCHHCCCCCCCHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RHFEMMR
CHHHHCC
>Mature Secondary Structure 
SADAAVKYVTEITPEDESRVAEWINTEEYKEKNFARQALVINPAHTCQPLGAELVAHSF
CCCHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHEEEECCHHHCCHHHHHHHHHHC
EGTLPFVHGSQGCASYYRSTLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEALENAYALY
CCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHHH
KPKMIGIFTSCMPEVIGDDLTAFIKNARQKGFIPQDFPVAYANTPSFNGSHIHGYDSMLL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEECCHHHHHH
SMLQTLTEGKRMEGRCTGKLNLIPGCDFNTGNYREYKRILKEFGIPATVLADISDVFDSP
HHHHHHHCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCC
LDGTYRLYPGGTKLEDAADSINGKVTMTLGKFATTRTFSWIKDNYAGEHLSLPTPFGIEK
CCCEEEECCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SDAFMMKLAELFGKPVPESLKAERGRAVDAMTDAHQYIHNKKFAVYGDPDYLLGYVSFLL
HHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH
EMGAQPYHILCSKGSKKLEKDIQALLDSSLYGKDAKIYMNKDLWHLRSLVMTDPVDAIIG
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHC
DTHGKFISRDAKIPLFRFGFPIFDRVNLHRSPLIGYQGVINMVTTICNKFIDITDDTCDD
CCCCCHHCCCCCCCHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RHFEMMR
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]