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The map label for this gene is nifD [H]

Identifier: 118581868

GI number: 118581868

Start: 3830879

End: 3832324

Strand: Direct

Name: nifD [H]

Synonym: Ppro_3468

Alternate gene names: 118581868

Gene position: 3830879-3832324 (Clockwise)

Preceding gene: 118581867

Following gene: 118581869

Centisome position: 95.58

GC content: 59.89

Gene sequence:

>1446_bases
ATGTCATCGACAGCGAGAAAGATAGAAGGGATCACCAAGGAATCCACCCAGGAGATGATCGACAAGGCCCTGGAAGCCTA
TCCCGAGAAGGGGAGAAAGAAACGTGCCCCCCATCTGGCGCCCAACGACCCGGGTGCAAGCTCGGCCTGCGTCAAGTCCA
ACCGCAAGACCGTGCCGGGCGTGATGAGCGCCCGCGGCTGCGCCTATGCCGGCGCCAAGGGTGTGGTCTGGGGCCCGATC
CGCGACATGGTGCATGTCTCCCACGGTCCGGTGGGATGCGGCTGGTACTCCTGGGGTACCCGGCGCAACATGATGACCGG
CATCACCGGCGTCAGTAACTTCGCCATGCAGTTCACCTCCGACTTCCAGGAGAAGGACATCGTCTACGGTGGCGACAAGA
AACTGATGCAGCTCCTGAGCGAGACCAAGGAACTCTTTCCCCTGGCCAAGGGGATCTCGGTCCTGTCCGAATGCCCGGTG
GGCCTGATCGGCGATGATATCAACAACGTTGCCCGCCAGGCGGTCAAGGAGCTGGATCTTCCCGTCATCCCCTGCAACTG
CGAGGGATTCCGCGGCGTCTCCCAGTCCCTGGGGCACCACATCTCCAACGACACCATCCGCGACCACATCATCGGTACCC
GCGAATTCGCCGAGCCCGAGACCCCCTATGACATCGCCCTGATCGGGGAATACAACATCGGCGGCGATGCCTGGTCGGTC
AAGCCGATGCTGGAGGAGATCGGGCTCAACGTCAAGGCTGTCTGGACCGGCGACGGTCAGCTGGAGCACATCGCCGCCAC
CCACAAGGTCAAGCTGAACCTGATCCACTGCTACCGCTCCATGAACTACATGTGCAAGGTTATGGAGGAAAAGTACGGTA
TCCCCTGGCTGGAGATCAACTTCTTCGGCCCCAGCAAGATCAGGGAGAGTCTGCGCAAGATCGCCGCACTGTTCGACGAC
ACCATCAAGGAGAACGTGGAGAAGGTCATTGCCAAATACGACCCCATGATGCAGGCCGTAATCGACGAATACCGCCCCCG
CCTGGATGGTAAGAAGGTCATGCTCTACGTGGGCGGCCTGCGCCCCCGCCACACAGTCAGCGCCTATGAGGAATTGGGCA
TGGACGTGGTCGGTTCAGGCTACGAATTCGCCCACGGCGACGACTACGAGCGCACCAGCGCCGAGATGCCCGAGGCAACG
GTGATCTACGACGACGTGTCCGAGTACGAACTGGAGGAGTTCGTCCACAAGCTCCGTCCAGATCTGGTGGGAAGCGGTAT
CAAGGAAAAATACCTGTTGCAGAAGATGGGGATACCGTTCCGCCAGATGCACAGCTGGGACTACTCCGGCCCCTATGAGG
GGTATAAAGGCTTTGAAGTTTTCGCGCGGGACATCGATATGGCGGTGAACAGCCCGACATGGAAATTCGTCAAGAGCCCG
TTTTAA

Upstream 100 bases:

>100_bases
ACTCCGCGCCCCTCGTCTATCAACGCGAGCGACCTGCGCGCACCACTGGAAATAAGCACAAAAAACTAATTGGTTACACA
CCAATCACAAGGAGTACCCA

Downstream 100 bases:

>100_bases
AACCGGTGAGGAGTGACGTGTGAGGCGTCATGGGGAACTCCCTGTTTTTACCGCCTCACGCCTCGCCCATCACCCCTCAC
CTTTCACATTTCCGACCGTG

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSSTARKIEGITKESTQEMIDKALEAYPEKGRKKRAPHLAPNDPGASSACVKSNRKTVPGVMSARGCAYAGAKGVVWGPI
RDMVHVSHGPVGCGWYSWGTRRNMMTGITGVSNFAMQFTSDFQEKDIVYGGDKKLMQLLSETKELFPLAKGISVLSECPV
GLIGDDINNVARQAVKELDLPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGEYNIGGDAWSV
KPMLEEIGLNVKAVWTGDGQLEHIAATHKVKLNLIHCYRSMNYMCKVMEEKYGIPWLEINFFGPSKIRESLRKIAALFDD
TIKENVEKVIAKYDPMMQAVIDEYRPRLDGKKVMLYVGGLRPRHTVSAYEELGMDVVGSGYEFAHGDDYERTSAEMPEAT
VIYDDVSEYELEEFVHKLRPDLVGSGIKEKYLLQKMGIPFRQMHSWDYSGPYEGYKGFEVFARDIDMAVNSPTWKFVKSP
F

Sequences:

>Translated_481_residues
MSSTARKIEGITKESTQEMIDKALEAYPEKGRKKRAPHLAPNDPGASSACVKSNRKTVPGVMSARGCAYAGAKGVVWGPI
RDMVHVSHGPVGCGWYSWGTRRNMMTGITGVSNFAMQFTSDFQEKDIVYGGDKKLMQLLSETKELFPLAKGISVLSECPV
GLIGDDINNVARQAVKELDLPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGEYNIGGDAWSV
KPMLEEIGLNVKAVWTGDGQLEHIAATHKVKLNLIHCYRSMNYMCKVMEEKYGIPWLEINFFGPSKIRESLRKIAALFDD
TIKENVEKVIAKYDPMMQAVIDEYRPRLDGKKVMLYVGGLRPRHTVSAYEELGMDVVGSGYEFAHGDDYERTSAEMPEAT
VIYDDVSEYELEEFVHKLRPDLVGSGIKEKYLLQKMGIPFRQMHSWDYSGPYEGYKGFEVFARDIDMAVNSPTWKFVKSP
F
>Mature_480_residues
SSTARKIEGITKESTQEMIDKALEAYPEKGRKKRAPHLAPNDPGASSACVKSNRKTVPGVMSARGCAYAGAKGVVWGPIR
DMVHVSHGPVGCGWYSWGTRRNMMTGITGVSNFAMQFTSDFQEKDIVYGGDKKLMQLLSETKELFPLAKGISVLSECPVG
LIGDDINNVARQAVKELDLPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGEYNIGGDAWSVK
PMLEEIGLNVKAVWTGDGQLEHIAATHKVKLNLIHCYRSMNYMCKVMEEKYGIPWLEINFFGPSKIRESLRKIAALFDDT
IKENVEKVIAKYDPMMQAVIDEYRPRLDGKKVMLYVGGLRPRHTVSAYEELGMDVVGSGYEFAHGDDYERTSAEMPEATV
IYDDVSEYELEEFVHKLRPDLVGSGIKEKYLLQKMGIPFRQMHSWDYSGPYEGYKGFEVFARDIDMAVNSPTWKFVKSPF

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 53868; Mature: 53737

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTARKIEGITKESTQEMIDKALEAYPEKGRKKRAPHLAPNDPGASSACVKSNRKTVPG
CCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCHHHHCCCCCCCC
VMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNMMTGITGVSNFAMQFTS
HHHCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DFQEKDIVYGGDKKLMQLLSETKELFPLAKGISVLSECPVGLIGDDINNVARQAVKELDL
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
PVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGEYNIGGDAWSV
CEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCCEEH
KPMLEEIGLNVKAVWTGDGQLEHIAATHKVKLNLIHCYRSMNYMCKVMEEKYGIPWLEIN
HHHHHHCCCCEEEEECCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FFGPSKIRESLRKIAALFDDTIKENVEKVIAKYDPMMQAVIDEYRPRLDGKKVMLYVGGL
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
RPRHTVSAYEELGMDVVGSGYEFAHGDDYERTSAEMPEATVIYDDVSEYELEEFVHKLRP
CCCHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHCCCCCCEEEECCCHHHHHHHHHHHHCH
DLVGSGIKEKYLLQKMGIPFRQMHSWDYSGPYEGYKGFEVFARDIDMAVNSPTWKFVKSP
HHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCC
F
C
>Mature Secondary Structure 
SSTARKIEGITKESTQEMIDKALEAYPEKGRKKRAPHLAPNDPGASSACVKSNRKTVPG
CCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCHHHHCCCCCCCC
VMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNMMTGITGVSNFAMQFTS
HHHCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DFQEKDIVYGGDKKLMQLLSETKELFPLAKGISVLSECPVGLIGDDINNVARQAVKELDL
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
PVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGEYNIGGDAWSV
CEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCCEEH
KPMLEEIGLNVKAVWTGDGQLEHIAATHKVKLNLIHCYRSMNYMCKVMEEKYGIPWLEIN
HHHHHHCCCCEEEEECCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FFGPSKIRESLRKIAALFDDTIKENVEKVIAKYDPMMQAVIDEYRPRLDGKKVMLYVGGL
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
RPRHTVSAYEELGMDVVGSGYEFAHGDDYERTSAEMPEATVIYDDVSEYELEEFVHKLRP
CCCHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHCCCCCCEEEECCCHHHHHHHHHHHHCH
DLVGSGIKEKYLLQKMGIPFRQMHSWDYSGPYEGYKGFEVFARDIDMAVNSPTWKFVKSP
HHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA