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The map label for this gene is aksA [H]

Identifier: 118581866

GI number: 118581866

Start: 3828454

End: 3829623

Strand: Direct

Name: aksA [H]

Synonym: Ppro_3466

Alternate gene names: 118581866

Gene position: 3828454-3829623 (Clockwise)

Preceding gene: 118581864

Following gene: 118581867

Centisome position: 95.52

GC content: 62.56

Gene sequence:

>1170_bases
ATGAGAGAACAGGCAGGCAGCACGAATATCATTATCGACGACACCACCCTGAGGGACGGAGAACAGACCGCCGGCGTGGT
CTTCAGCAAACGGGACAAGATCGCCATAGCACGCATGCTGGATTCCATCGGTGTCCAGGAACTGGAATGCGGCATTCCGG
CCATGGGAAGGGAAGAACGCGAAGGGATCAGGGCACTGGTTGACCTGGGGCTCAATGCCCGCCTGATCGCCTGGAACCGG
GCGCTGGTCCCCGATATCCAGGCCAGCATCGACTGCGGCATATCGGCGGTGGATATCTCGCTGCCGGTATCGGACATCAT
GATTCGCAACAAGATCCGCAAGAGCCGCTCCTGGGTACGGGAACAGCTCAAAACAGCGCTGGGCTTTGCCAAGCAGCACG
GCTTGTACGTATCGGTGGGGGGCGAGGACGCCAGCCGCGCCGACCATGCCTTTCTGGTGGAGTTGATGCGCATAACACTG
CAGATGGGGGGAGACCGTTTCCGCTTCTGCGACACCCTGGGGATCCTCGACCCCTTCGGCATGTACGACAGGATACGCGC
CCTGCGTGAGGCGGTGCCGGAACTGGCCATCGAGGTGCATACCCACAACGACTTCGGCATGGCCACCGCCAACACCATCG
CCGGTATCCGCGCCGGCGCCACCTTTGCCAATACCACCGTGAACGGCTTGGGAGAGCGGGCCGGCAATGCCGCCCTTGAG
GAGGTGGTCATGGGTCTCAGGCACGCCTGCGCTATGGATACCGGTATTGCCAGCCATCGTTTCCGCGAGCTGTCGCTCTT
CGTTTCCCGGGCCTCCCGCCGCCCCCTGCCCCCCTGGAAGGCAGTAGTGGGGGAGTCGGTCTTTGCCCACGAGTCAGGCC
TGCACACCGACGGGGTGCTTAAAAATCCGTCAAACTACGAGGGATTCGACCCGGCAGAGGTGGGGTTGAGCCGCAGCATC
GTCGTCGGCAAGCACTCCGGCAGTAGCGGCCTGATCGACCGCTACCGCGCCATGGGTATCGCCGTCAGCCGTTCAGAGGC
TACCCGCCTGGTGGAAAGGGTCAGGAGAGTCGCCCAGAGGACCAGGCGGCCGCTCACTAACGAGCAGTTGCTCAGACTGT
ACGACGGCAGAGGGGCGGAAATCCCCGCAAACAGTTTCTGTGTCTGCTGA

Upstream 100 bases:

>100_bases
AGAGTGGTACGCCCCGTGCATGGCATGGGCAGGTTTTGCATCCGGCAACGGCGCCGCCATTCCATGTATGGAGGCGCTTT
GCTTTATTCAGGGGGAGCCC

Downstream 100 bases:

>100_bases
GGTGTCTGTGCACAGTAATTGTGCAAACCGATGCCCACAGGCAAGCTTCACACACATCAACCAGCACCGGACACAACAAA
AACAGTAAGTTACAATTTGG

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MREQAGSTNIIIDDTTLRDGEQTAGVVFSKRDKIAIARMLDSIGVQELECGIPAMGREEREGIRALVDLGLNARLIAWNR
ALVPDIQASIDCGISAVDISLPVSDIMIRNKIRKSRSWVREQLKTALGFAKQHGLYVSVGGEDASRADHAFLVELMRITL
QMGGDRFRFCDTLGILDPFGMYDRIRALREAVPELAIEVHTHNDFGMATANTIAGIRAGATFANTTVNGLGERAGNAALE
EVVMGLRHACAMDTGIASHRFRELSLFVSRASRRPLPPWKAVVGESVFAHESGLHTDGVLKNPSNYEGFDPAEVGLSRSI
VVGKHSGSSGLIDRYRAMGIAVSRSEATRLVERVRRVAQRTRRPLTNEQLLRLYDGRGAEIPANSFCVC

Sequences:

>Translated_389_residues
MREQAGSTNIIIDDTTLRDGEQTAGVVFSKRDKIAIARMLDSIGVQELECGIPAMGREEREGIRALVDLGLNARLIAWNR
ALVPDIQASIDCGISAVDISLPVSDIMIRNKIRKSRSWVREQLKTALGFAKQHGLYVSVGGEDASRADHAFLVELMRITL
QMGGDRFRFCDTLGILDPFGMYDRIRALREAVPELAIEVHTHNDFGMATANTIAGIRAGATFANTTVNGLGERAGNAALE
EVVMGLRHACAMDTGIASHRFRELSLFVSRASRRPLPPWKAVVGESVFAHESGLHTDGVLKNPSNYEGFDPAEVGLSRSI
VVGKHSGSSGLIDRYRAMGIAVSRSEATRLVERVRRVAQRTRRPLTNEQLLRLYDGRGAEIPANSFCVC
>Mature_389_residues
MREQAGSTNIIIDDTTLRDGEQTAGVVFSKRDKIAIARMLDSIGVQELECGIPAMGREEREGIRALVDLGLNARLIAWNR
ALVPDIQASIDCGISAVDISLPVSDIMIRNKIRKSRSWVREQLKTALGFAKQHGLYVSVGGEDASRADHAFLVELMRITL
QMGGDRFRFCDTLGILDPFGMYDRIRALREAVPELAIEVHTHNDFGMATANTIAGIRAGATFANTTVNGLGERAGNAALE
EVVMGLRHACAMDTGIASHRFRELSLFVSRASRRPLPPWKAVVGESVFAHESGLHTDGVLKNPSNYEGFDPAEVGLSRSI
VVGKHSGSSGLIDRYRAMGIAVSRSEATRLVERVRRVAQRTRRPLTNEQLLRLYDGRGAEIPANSFCVC

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=278, Percent_Identity=25.8992805755396, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786261, Length=387, Percent_Identity=31.266149870801, Blast_Score=174, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6320071, Length=361, Percent_Identity=27.4238227146814, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=353, Percent_Identity=27.1954674220963, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324682, Length=406, Percent_Identity=21.9211822660099, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582381, Length=272, Percent_Identity=24.6323529411765, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42551; Mature: 42551

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREQAGSTNIIIDDTTLRDGEQTAGVVFSKRDKIAIARMLDSIGVQELECGIPAMGREER
CCCCCCCCEEEEECCCCCCCCHHCCEEEECCCHHHHHHHHHHCCHHHHHCCCCCCCCHHH
EGIRALVDLGLNARLIAWNRALVPDIQASIDCGISAVDISLPVSDIMIRNKIRKSRSWVR
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
EQLKTALGFAKQHGLYVSVGGEDASRADHAFLVELMRITLQMGGDRFRFCDTLGILDPFG
HHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCC
MYDRIRALREAVPELAIEVHTHNDFGMATANTIAGIRAGATFANTTVNGLGERAGNAALE
HHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EVVMGLRHACAMDTGIASHRFRELSLFVSRASRRPLPPWKAVVGESVFAHESGLHTDGVL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCCCCCCCCC
KNPSNYEGFDPAEVGLSRSIVVGKHSGSSGLIDRYRAMGIAVSRSEATRLVERVRRVAQR
CCCCCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
TRRPLTNEQLLRLYDGRGAEIPANSFCVC
HHCCCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MREQAGSTNIIIDDTTLRDGEQTAGVVFSKRDKIAIARMLDSIGVQELECGIPAMGREER
CCCCCCCCEEEEECCCCCCCCHHCCEEEECCCHHHHHHHHHHCCHHHHHCCCCCCCCHHH
EGIRALVDLGLNARLIAWNRALVPDIQASIDCGISAVDISLPVSDIMIRNKIRKSRSWVR
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
EQLKTALGFAKQHGLYVSVGGEDASRADHAFLVELMRITLQMGGDRFRFCDTLGILDPFG
HHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCC
MYDRIRALREAVPELAIEVHTHNDFGMATANTIAGIRAGATFANTTVNGLGERAGNAALE
HHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EVVMGLRHACAMDTGIASHRFRELSLFVSRASRRPLPPWKAVVGESVFAHESGLHTDGVL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCCCCCCCCC
KNPSNYEGFDPAEVGLSRSIVVGKHSGSSGLIDRYRAMGIAVSRSEATRLVERVRRVAQR
CCCCCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
TRRPLTNEQLLRLYDGRGAEIPANSFCVC
HHCCCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]