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The map label for this gene is pcrA [H]

Identifier: 118581858

GI number: 118581858

Start: 3816485

End: 3818773

Strand: Reverse

Name: pcrA [H]

Synonym: Ppro_3458

Alternate gene names: 118581858

Gene position: 3818773-3816485 (Counterclockwise)

Preceding gene: 118581862

Following gene: 118581857

Centisome position: 95.28

GC content: 61.6

Gene sequence:

>2289_bases
ATGGATTTTGTCAAACACTTAAACCAGCCCCAGAAAAGCGCGGTGCTGCATGGAGAAGGACCGCTGCTGATCCTGGCCGG
CGCCGGCTCGGGCAAGACCCGCGTCATCACCCACCGTATCGCGCACCTGATCCACAATCATGGGGTGCGTCCCTGGAATA
TCCTGGCGGTGACCTTCACCAACAAGGCAGCCAGGGAGATGTCCGAACGGGTCAGCAGGCTGCTGGGGGGAGGCGATGCG
CCGCTGATCGCCACATTCCATGCCGCCTGCGGCCGGATCCTGCGCCGCGAGATCCACCACCTGGGCTTCGACTCCAGCTT
TGCCATCTACGACGAGCGTGATTGCGAACGGCTACTGAAGGATCTGCTGAAGGATATGGACCTGGACGATAAGAAGTTTT
CGCCCAGGATCGTTGCAGCCCGTATCGATGACTACAAGAACCGCGGCCTGTTTCCCCATGACCTGGATCCCGTGGCAACC
GGTGACATCTTCAACGCCAAGGTTGTCCAGATCTATGCTGCCTACCAGGAACGGCTGAGAAAATGCAACGCACTTGATTT
CGGCGACATGCTGATCCAGACCGTGCGCCTGCTGGAACAGTTTCCCGAGGTGCGTCAACGCTGCCAGGAACGTTTCCAGT
GGATCATGGTGGACGAATACCAGGACACCAACCCGGTTCAGTACCGGCTGATCCGGCTTCTGGCCGGAGAACGTAGGAAT
CTTTGCGTGGTGGGTGACGACGACCAGTCGATCTACTCCTGGCGCGGGGCGGATATTCGCAACATACTGGAATTCGAGAA
GGACTTCCCGGACGTGGCGGTCGTGCGGCTGGAGCAGAACTACCGCTCCACCACCACCATCCTCAAGGCGGCCGGCGCGG
TGGTCAGCCGAAATATCGGCCGTCGGGGCAAGACCCTCTGGACCGAGAATCCGGAGGGAGAGAAGATCCGCTACCACCGG
GTAGAATCGGACCGGGAGGAGGCGCGCCTCGTGGCCCGAGAAATCGTCAGCCTGCGCTCACGCGGTATCCCGCTGGAGGA
GATGGCGGTCTTCTACCGCACCAATGCCCAGTCCCGCCTGGTGGAGGAGGCGCTGGTTTCCGAAGCGCTTCCCTACCATA
TCGTGGGGGGCGTACGTTTCTATGCCCGCATGGAGGTCAAGGACATCCTGGCCTATCTGCGGATACTGGACAACCCGGCC
GACGAGATTTCGCTGAAACGGATCATCAACGTGCCGAGCCGGGGCATTGGCGGGGCAACCATCGACCGCATCTCCCAGCG
GGTAGCCTCAAGCGGCGCCAGCTTTTACGCTGCCATGGCCGAGTGTGCCGGGAGCGGGCTGCTGGGTTCCGGGCCGCGTG
CCAGGGTCGCGTCGTTTGTCGACATGATGGAACGTTTCAGGGAGATGGTTGCCCTGCGCGGTTTGCCTGAACTGTGTCAG
ACTGTCATGGAGGAGAGCGGCTATCTGGCTCGCCTGCGGGAGAGTCGCGACCAGGAGGACAGCGAGCGACTTGAGAACCT
GGAACAGCTGCTGGCGGCAATGGAGGAGTTCTGTGAGAAGGAGCCCCAGGCCGGCCTGTCGGCGTTCCTGGAGCAGGTCT
CGCTGGTTTCGGACCTGGAGCAGGGGGGGCAGGGCAAGCCATCGGTGACCCTGATGACCCTGCATGCCGCCAAGGGTCTT
GAGTTCAGGGCGGTTTTCATGATCGGTATGGAGGAGCGCCTCTTTCCCCATGTGCGTGCCCTGGATGACCTGGACGGAAT
GGAAGAGGAGCGTCGCCTCTGTTACGTCGGCATGACCCGCGCTCGCGAGCGACTCTACCTGCTCAATACCCGCCGAAGGT
ACCTGTTCGGACAGGAACAGTGCAATCCGCCGTCACGCTTCCTGAAGGATATTCCGGCCGGATTGCTGGATGACGGGGGA
GAACCGAGCCAGCCCGGTCTTGGGTTCCGCTCGGCGGCGGAACAGGCCGGCAGAAATGGGCAAGAGCCCTCTACGGGCAT
TATCGCCATGGGCCACTCCGGTCGTCGGGAGAAGACTGTTGCCCGGCATGACGGCGGACACAATCTGGCACAGGTCGTTT
CAGCATGCGATGAAATCGAAGTCATTCCCGAGCCGCCCGAAGAGCACGACGGCGTCTTTATCGGCATGAAGGTCCGGCAT
GGCACGTTCGGGCAGGGTACGATCCGCAAACTCGAGGGAAGCGGCGAAAGCCAGAAGGTCATTGTCTGGTTCAATTCGGT
TGGCCCCAAGAAGCTCATGCTGCGCTTCGCGGGCCTGGAGCGGGCCTGA

Upstream 100 bases:

>100_bases
CGGCGTCCGATGACGGAGCGTGGAAAAACGGGCAGCGGCACTGCTTCCCGGCCGGCTTTTTTCGTTGACGCTCCCGCGGT
GAGGGACTACAAATGACGGC

Downstream 100 bases:

>100_bases
CAACGCACTACGGGGGGAACCATGGATATTCTGGACGCAACGGTTGCCGATGCTGCCGAGATCATTGAACTGCAGAAACT
GGCCTTTCTCTCCGAGGCGC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MDFVKHLNQPQKSAVLHGEGPLLILAGAGSGKTRVITHRIAHLIHNHGVRPWNILAVTFTNKAAREMSERVSRLLGGGDA
PLIATFHAACGRILRREIHHLGFDSSFAIYDERDCERLLKDLLKDMDLDDKKFSPRIVAARIDDYKNRGLFPHDLDPVAT
GDIFNAKVVQIYAAYQERLRKCNALDFGDMLIQTVRLLEQFPEVRQRCQERFQWIMVDEYQDTNPVQYRLIRLLAGERRN
LCVVGDDDQSIYSWRGADIRNILEFEKDFPDVAVVRLEQNYRSTTTILKAAGAVVSRNIGRRGKTLWTENPEGEKIRYHR
VESDREEARLVAREIVSLRSRGIPLEEMAVFYRTNAQSRLVEEALVSEALPYHIVGGVRFYARMEVKDILAYLRILDNPA
DEISLKRIINVPSRGIGGATIDRISQRVASSGASFYAAMAECAGSGLLGSGPRARVASFVDMMERFREMVALRGLPELCQ
TVMEESGYLARLRESRDQEDSERLENLEQLLAAMEEFCEKEPQAGLSAFLEQVSLVSDLEQGGQGKPSVTLMTLHAAKGL
EFRAVFMIGMEERLFPHVRALDDLDGMEEERRLCYVGMTRARERLYLLNTRRRYLFGQEQCNPPSRFLKDIPAGLLDDGG
EPSQPGLGFRSAAEQAGRNGQEPSTGIIAMGHSGRREKTVARHDGGHNLAQVVSACDEIEVIPEPPEEHDGVFIGMKVRH
GTFGQGTIRKLEGSGESQKVIVWFNSVGPKKLMLRFAGLERA

Sequences:

>Translated_762_residues
MDFVKHLNQPQKSAVLHGEGPLLILAGAGSGKTRVITHRIAHLIHNHGVRPWNILAVTFTNKAAREMSERVSRLLGGGDA
PLIATFHAACGRILRREIHHLGFDSSFAIYDERDCERLLKDLLKDMDLDDKKFSPRIVAARIDDYKNRGLFPHDLDPVAT
GDIFNAKVVQIYAAYQERLRKCNALDFGDMLIQTVRLLEQFPEVRQRCQERFQWIMVDEYQDTNPVQYRLIRLLAGERRN
LCVVGDDDQSIYSWRGADIRNILEFEKDFPDVAVVRLEQNYRSTTTILKAAGAVVSRNIGRRGKTLWTENPEGEKIRYHR
VESDREEARLVAREIVSLRSRGIPLEEMAVFYRTNAQSRLVEEALVSEALPYHIVGGVRFYARMEVKDILAYLRILDNPA
DEISLKRIINVPSRGIGGATIDRISQRVASSGASFYAAMAECAGSGLLGSGPRARVASFVDMMERFREMVALRGLPELCQ
TVMEESGYLARLRESRDQEDSERLENLEQLLAAMEEFCEKEPQAGLSAFLEQVSLVSDLEQGGQGKPSVTLMTLHAAKGL
EFRAVFMIGMEERLFPHVRALDDLDGMEEERRLCYVGMTRARERLYLLNTRRRYLFGQEQCNPPSRFLKDIPAGLLDDGG
EPSQPGLGFRSAAEQAGRNGQEPSTGIIAMGHSGRREKTVARHDGGHNLAQVVSACDEIEVIPEPPEEHDGVFIGMKVRH
GTFGQGTIRKLEGSGESQKVIVWFNSVGPKKLMLRFAGLERA
>Mature_762_residues
MDFVKHLNQPQKSAVLHGEGPLLILAGAGSGKTRVITHRIAHLIHNHGVRPWNILAVTFTNKAAREMSERVSRLLGGGDA
PLIATFHAACGRILRREIHHLGFDSSFAIYDERDCERLLKDLLKDMDLDDKKFSPRIVAARIDDYKNRGLFPHDLDPVAT
GDIFNAKVVQIYAAYQERLRKCNALDFGDMLIQTVRLLEQFPEVRQRCQERFQWIMVDEYQDTNPVQYRLIRLLAGERRN
LCVVGDDDQSIYSWRGADIRNILEFEKDFPDVAVVRLEQNYRSTTTILKAAGAVVSRNIGRRGKTLWTENPEGEKIRYHR
VESDREEARLVAREIVSLRSRGIPLEEMAVFYRTNAQSRLVEEALVSEALPYHIVGGVRFYARMEVKDILAYLRILDNPA
DEISLKRIINVPSRGIGGATIDRISQRVASSGASFYAAMAECAGSGLLGSGPRARVASFVDMMERFREMVALRGLPELCQ
TVMEESGYLARLRESRDQEDSERLENLEQLLAAMEEFCEKEPQAGLSAFLEQVSLVSDLEQGGQGKPSVTLMTLHAAKGL
EFRAVFMIGMEERLFPHVRALDDLDGMEEERRLCYVGMTRARERLYLLNTRRRYLFGQEQCNPPSRFLKDIPAGLLDDGG
EPSQPGLGFRSAAEQAGRNGQEPSTGIIAMGHSGRREKTVARHDGGHNLAQVVSACDEIEVIPEPPEEHDGVFIGMKVRH
GTFGQGTIRKLEGSGESQKVIVWFNSVGPKKLMLRFAGLERA

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=766, Percent_Identity=37.9895561357702, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI48994965, Length=641, Percent_Identity=39.1575663026521, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787196, Length=338, Percent_Identity=28.4023668639053, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=735, Percent_Identity=28.4353741496599, Blast_Score=243, Evalue=8e-65,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85742; Mature: 85742

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVKHLNQPQKSAVLHGEGPLLILAGAGSGKTRVITHRIAHLIHNHGVRPWNILAVTFT
CCHHHHCCCCCHHCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAREMSERVSRLLGGGDAPLIATFHAACGRILRREIHHLGFDSSFAIYDERDCERLLK
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHH
DLLKDMDLDDKKFSPRIVAARIDDYKNRGLFPHDLDPVATGDIFNAKVVQIYAAYQERLR
HHHHHCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KCNALDFGDMLIQTVRLLEQFPEVRQRCQERFQWIMVDEYQDTNPVQYRLIRLLAGERRN
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCC
LCVVGDDDQSIYSWRGADIRNILEFEKDFPDVAVVRLEQNYRSTTTILKAAGAVVSRNIG
EEEECCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
RRGKTLWTENPEGEKIRYHRVESDREEARLVAREIVSLRSRGIPLEEMAVFYRTNAQSRL
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
VEEALVSEALPYHIVGGVRFYARMEVKDILAYLRILDNPADEISLKRIINVPSRGIGGAT
HHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHH
IDRISQRVASSGASFYAAMAECAGSGLLGSGPRARVASFVDMMERFREMVALRGLPELCQ
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TVMEESGYLARLRESRDQEDSERLENLEQLLAAMEEFCEKEPQAGLSAFLEQVSLVSDLE
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QGGQGKPSVTLMTLHAAKGLEFRAVFMIGMEERLFPHVRALDDLDGMEEERRLCYVGMTR
HCCCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHEEHHHHHH
ARERLYLLNTRRRYLFGQEQCNPPSRFLKDIPAGLLDDGGEPSQPGLGFRSAAEQAGRNG
HHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHCCCC
QEPSTGIIAMGHSGRREKTVARHDGGHNLAQVVSACDEIEVIPEPPEEHDGVFIGMKVRH
CCCCCCEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHCCEECCCCCCCCCCEEEEEEEEC
GTFGQGTIRKLEGSGESQKVIVWFNSVGPKKLMLRFAGLERA
CCCCCCHHHEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MDFVKHLNQPQKSAVLHGEGPLLILAGAGSGKTRVITHRIAHLIHNHGVRPWNILAVTFT
CCHHHHCCCCCHHCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAREMSERVSRLLGGGDAPLIATFHAACGRILRREIHHLGFDSSFAIYDERDCERLLK
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHH
DLLKDMDLDDKKFSPRIVAARIDDYKNRGLFPHDLDPVATGDIFNAKVVQIYAAYQERLR
HHHHHCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KCNALDFGDMLIQTVRLLEQFPEVRQRCQERFQWIMVDEYQDTNPVQYRLIRLLAGERRN
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCC
LCVVGDDDQSIYSWRGADIRNILEFEKDFPDVAVVRLEQNYRSTTTILKAAGAVVSRNIG
EEEECCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
RRGKTLWTENPEGEKIRYHRVESDREEARLVAREIVSLRSRGIPLEEMAVFYRTNAQSRL
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
VEEALVSEALPYHIVGGVRFYARMEVKDILAYLRILDNPADEISLKRIINVPSRGIGGAT
HHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHH
IDRISQRVASSGASFYAAMAECAGSGLLGSGPRARVASFVDMMERFREMVALRGLPELCQ
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TVMEESGYLARLRESRDQEDSERLENLEQLLAAMEEFCEKEPQAGLSAFLEQVSLVSDLE
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QGGQGKPSVTLMTLHAAKGLEFRAVFMIGMEERLFPHVRALDDLDGMEEERRLCYVGMTR
HCCCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHEEHHHHHH
ARERLYLLNTRRRYLFGQEQCNPPSRFLKDIPAGLLDDGGEPSQPGLGFRSAAEQAGRNG
HHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHCCCC
QEPSTGIIAMGHSGRREKTVARHDGGHNLAQVVSACDEIEVIPEPPEEHDGVFIGMKVRH
CCCCCCEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHCCEECCCCCCCCCCEEEEEEEEC
GTFGQGTIRKLEGSGESQKVIVWFNSVGPKKLMLRFAGLERA
CCCCCCHHHEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]