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The map label for this gene is capI [H]

Identifier: 118581806

GI number: 118581806

Start: 3760665

End: 3761705

Strand: Reverse

Name: capI [H]

Synonym: Ppro_3406

Alternate gene names: 118581806

Gene position: 3761705-3760665 (Counterclockwise)

Preceding gene: 118581808

Following gene: 118581805

Centisome position: 93.85

GC content: 63.02

Gene sequence:

>1041_bases
ATGAGCACATGCGGATTTGATACGGGCAACAGGGTTCTGGTCACCGGCGCGGCCGGATTCATCGGTTTTCACTTAAGCAG
GCACTTGCTGGAGCAGGGGTACCAGGTGATCGGCCTGGACAACCTGAACGACTACTACGACCAGACCCTCAAGGAGGCTC
GCCTGCGCTTGCTGGAGCCGCTGCCCGGCTTCAGTTTCCTGCGCGCCTCCCTGGAGGACGCTCGGCAGATGGAGGAGCTG
TTCAGCCGGGAGCGCTTCGACCTGGTGGTCAATCTGGCTGCCCAGGCCGGCGTACGCTACTCCATCACCAACCCCCACGC
CTACATCTCCAGCAATGTTGCCGGGTTCCTGAACGTACTGGAGGGATGCCGCCACACCGGCGTCAAACACCTGGTTTTCG
CCTCCTCCAGCTCCGTATACGGCGCCAACGCCCGGGTCCCCTTTTCGGAGCACCATACGGTGGATCACCCGGTTTCCCTC
TATGCGGCCACCAAAAAATCCAACGAACTGATGGCCCACACCTATGCGCACCTTTTCGGCCTAGCGACAACCGGGCTGCG
TTTCTTCACCGTTTACGGACCCTGGGGGCGGCCGGACATGGCCTATTTCTCCTTTACCAAGGCCATCCTGGAGGGGCGGG
CCATCGACGTCTTCAACCACGGCCGGATGCGGCGCGATTTCACCTATATCGACGACATCGTCCAGGGGATCGCGCGGGTG
CTGGAGCGCCCCCCCCAAGGGGATGCGGCGTGGGACGCCAATGCGCCCGATCCGGCCAGCAGCTCCGCTCCCTACCGCAT
CTACAACATCGGCAACAACCGTCCGGTGGAGTTGGGGCGCTTCATCGAGACGCTGGAGCAGTTGCTGGGCAAAAAGGCGA
TCAAAAACATGCTGCCCATGCAGCCGGGCGACGTGCCGGCCACCTGCGCCGATATCGACGATCTGGCGCGGGATGCAGGC
TTCCGGCCCAGCACCCCCATCGAAACCGGGCTGCGCCGCTTCGTGGAATGGTACCGGGAGTACTATGGCGGTGCGGTGTG
A

Upstream 100 bases:

>100_bases
GTAATTTACTAATTTTGGGATTATAGTAACGCCGCTTCACTACCATTTCACCAGCGAGGGCGGCCGGCGCGTACAGAGTC
GGCCTGAAAAAACGGCATAT

Downstream 100 bases:

>100_bases
AAGGTCTGTTTTGAAAACCGGTCTCACGCAAAGCCGCCAAGACGCAAAGAAATAAGATAGTTGTCAAACAATGGCGTTTC
GCCACATAGGCGATTCAACT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSTCGFDTGNRVLVTGAAGFIGFHLSRHLLEQGYQVIGLDNLNDYYDQTLKEARLRLLEPLPGFSFLRASLEDARQMEEL
FSRERFDLVVNLAAQAGVRYSITNPHAYISSNVAGFLNVLEGCRHTGVKHLVFASSSSVYGANARVPFSEHHTVDHPVSL
YAATKKSNELMAHTYAHLFGLATTGLRFFTVYGPWGRPDMAYFSFTKAILEGRAIDVFNHGRMRRDFTYIDDIVQGIARV
LERPPQGDAAWDANAPDPASSSAPYRIYNIGNNRPVELGRFIETLEQLLGKKAIKNMLPMQPGDVPATCADIDDLARDAG
FRPSTPIETGLRRFVEWYREYYGGAV

Sequences:

>Translated_346_residues
MSTCGFDTGNRVLVTGAAGFIGFHLSRHLLEQGYQVIGLDNLNDYYDQTLKEARLRLLEPLPGFSFLRASLEDARQMEEL
FSRERFDLVVNLAAQAGVRYSITNPHAYISSNVAGFLNVLEGCRHTGVKHLVFASSSSVYGANARVPFSEHHTVDHPVSL
YAATKKSNELMAHTYAHLFGLATTGLRFFTVYGPWGRPDMAYFSFTKAILEGRAIDVFNHGRMRRDFTYIDDIVQGIARV
LERPPQGDAAWDANAPDPASSSAPYRIYNIGNNRPVELGRFIETLEQLLGKKAIKNMLPMQPGDVPATCADIDDLARDAG
FRPSTPIETGLRRFVEWYREYYGGAV
>Mature_345_residues
STCGFDTGNRVLVTGAAGFIGFHLSRHLLEQGYQVIGLDNLNDYYDQTLKEARLRLLEPLPGFSFLRASLEDARQMEELF
SRERFDLVVNLAAQAGVRYSITNPHAYISSNVAGFLNVLEGCRHTGVKHLVFASSSSVYGANARVPFSEHHTVDHPVSLY
AATKKSNELMAHTYAHLFGLATTGLRFFTVYGPWGRPDMAYFSFTKAILEGRAIDVFNHGRMRRDFTYIDDIVQGIARVL
ERPPQGDAAWDANAPDPASSSAPYRIYNIGNNRPVELGRFIETLEQLLGKKAIKNMLPMQPGDVPATCADIDDLARDAGF
RPSTPIETGLRRFVEWYREYYGGAV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=26.453488372093, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI56237023, Length=353, Percent_Identity=26.628895184136, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI56118217, Length=353, Percent_Identity=26.628895184136, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI189083684, Length=353, Percent_Identity=26.628895184136, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=26.3313609467456, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=25.6338028169014, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=25.3521126760563, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=24.929178470255, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788589, Length=254, Percent_Identity=27.5590551181102, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=26.0744985673352, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=25.6410256410256, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=25.8064516129032, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=25.0728862973761, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=342, Percent_Identity=20.1754385964912, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=143, Percent_Identity=36.3636363636364, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=345, Percent_Identity=27.8260869565217, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=25.5747126436782, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38594; Mature: 38463

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTCGFDTGNRVLVTGAAGFIGFHLSRHLLEQGYQVIGLDNLNDYYDQTLKEARLRLLEP
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCC
LPGFSFLRASLEDARQMEELFSRERFDLVVNLAAQAGVRYSITNPHAYISSNVAGFLNVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
EGCRHTGVKHLVFASSSSVYGANARVPFSEHHTVDHPVSLYAATKKSNELMAHTYAHLFG
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
LATTGLRFFTVYGPWGRPDMAYFSFTKAILEGRAIDVFNHGRMRRDFTYIDDIVQGIARV
HHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LERPPQGDAAWDANAPDPASSSAPYRIYNIGNNRPVELGRFIETLEQLLGKKAIKNMLPM
HCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
QPGDVPATCADIDDLARDAGFRPSTPIETGLRRFVEWYREYYGGAV
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STCGFDTGNRVLVTGAAGFIGFHLSRHLLEQGYQVIGLDNLNDYYDQTLKEARLRLLEP
CCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCC
LPGFSFLRASLEDARQMEELFSRERFDLVVNLAAQAGVRYSITNPHAYISSNVAGFLNVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
EGCRHTGVKHLVFASSSSVYGANARVPFSEHHTVDHPVSLYAATKKSNELMAHTYAHLFG
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
LATTGLRFFTVYGPWGRPDMAYFSFTKAILEGRAIDVFNHGRMRRDFTYIDDIVQGIARV
HHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LERPPQGDAAWDANAPDPASSSAPYRIYNIGNNRPVELGRFIETLEQLLGKKAIKNMLPM
HCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
QPGDVPATCADIDDLARDAGFRPSTPIETGLRRFVEWYREYYGGAV
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]