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The map label for this gene is mtnU [H]

Identifier: 118581727

GI number: 118581727

Start: 3663853

End: 3664623

Strand: Reverse

Name: mtnU [H]

Synonym: Ppro_3327

Alternate gene names: 118581727

Gene position: 3664623-3663853 (Counterclockwise)

Preceding gene: 118581728

Following gene: 118581726

Centisome position: 91.43

GC content: 58.63

Gene sequence:

>771_bases
ATGATAACCGCTGCCGCCATCCAGTTTACTATCAAGCCTGGCAATGTGGATGCAAACCTCGCCCATGCGCGGGACGCCCT
CGGGCGCGCCTCCGACCGGGGGGCTCAACTGGTCGTGCTGCCCGAAATGTGGTCCTGCGGTTTTTGCGGCAAAAGCCTGG
CTGGTCTTGCCATGCATACCGCGGCTATTGCCGAGGAATTGCAGGATCTTTCCCGCAGGCTGGGTTTAGTGGTTGTGGGA
AGCATGCCCGAACCAAACGGGGACAGGGTGTTCAACACAATCCATCTGATCGACAACGGCAGGCTTGCAGGAATCTACCG
CAAGGTCCACCTGTTTTCCCTGCTGGGGGAAGATCGCGTTTTTGCCGCTGGAGACGGCTGTCTGCTGGCGGATACATCGA
TCGGGAAAATCGGTGTCATCATCTGCTATGACCTGCGTTTTCCCGAGTTGTCACGACGTCTTGCCCTCGAGGGCGCTCAG
ATCCTCTGCATCCCGGCCCAATGGCCCAAGCCACGAAACGAACAGTGGCGAACACTGCTGCAGGCCCGGGCCATAGAGAA
CCAGTTCTTCGTTGTTGCATCCAACACCTGCGGCATGGTGGGCAAACTGGATTTCTTCGGCATGAGCATGATAATCGGCC
CCCGGGGGGACGTGCTTGCGGAGGCGGGAGATCGGGAAACGGAGATCGTTGCCGGCCTTGATATGCGGGATATGGCCGAG
TGGCGTGCTCAAATCCCCTGCTTCAAAGATCGCAGGCCGGAATTGTACTGA

Upstream 100 bases:

>100_bases
CAGGGCTCTTTTGCGTATGCATCCTGACGGCATGCGGCTACGGCGCTGTGTTCTCTCCCGTGGCGTGCCCCAAAAACAGG
ACCAGAAAGGAAGCTCCAGA

Downstream 100 bases:

>100_bases
TTTTGACTTGACTTGATTATCCGTTCGGACTAGCTTTCCCACATATTTCATCCTCCGGAGGTGCTCGTTGTGGCCCTGCT
GCTTAACGCCGGTATCGTCG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MITAAAIQFTIKPGNVDANLAHARDALGRASDRGAQLVVLPEMWSCGFCGKSLAGLAMHTAAIAEELQDLSRRLGLVVVG
SMPEPNGDRVFNTIHLIDNGRLAGIYRKVHLFSLLGEDRVFAAGDGCLLADTSIGKIGVIICYDLRFPELSRRLALEGAQ
ILCIPAQWPKPRNEQWRTLLQARAIENQFFVVASNTCGMVGKLDFFGMSMIIGPRGDVLAEAGDRETEIVAGLDMRDMAE
WRAQIPCFKDRRPELY

Sequences:

>Translated_256_residues
MITAAAIQFTIKPGNVDANLAHARDALGRASDRGAQLVVLPEMWSCGFCGKSLAGLAMHTAAIAEELQDLSRRLGLVVVG
SMPEPNGDRVFNTIHLIDNGRLAGIYRKVHLFSLLGEDRVFAAGDGCLLADTSIGKIGVIICYDLRFPELSRRLALEGAQ
ILCIPAQWPKPRNEQWRTLLQARAIENQFFVVASNTCGMVGKLDFFGMSMIIGPRGDVLAEAGDRETEIVAGLDMRDMAE
WRAQIPCFKDRRPELY
>Mature_256_residues
MITAAAIQFTIKPGNVDANLAHARDALGRASDRGAQLVVLPEMWSCGFCGKSLAGLAMHTAAIAEELQDLSRRLGLVVVG
SMPEPNGDRVFNTIHLIDNGRLAGIYRKVHLFSLLGEDRVFAAGDGCLLADTSIGKIGVIICYDLRFPELSRRLALEGAQ
ILCIPAQWPKPRNEQWRTLLQARAIENQFFVVASNTCGMVGKLDFFGMSMIIGPRGDVLAEAGDRETEIVAGLDMRDMAE
WRAQIPCFKDRRPELY

Specific function: May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=265, Percent_Identity=30.188679245283, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI297632350, Length=274, Percent_Identity=31.7518248175182, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI5031947, Length=274, Percent_Identity=31.7518248175182, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI297632348, Length=274, Percent_Identity=31.7518248175182, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1786412, Length=219, Percent_Identity=31.0502283105023, Blast_Score=109, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=260, Percent_Identity=33.0769230769231, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323383, Length=275, Percent_Identity=30.1818181818182, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322335, Length=291, Percent_Identity=27.4914089347079, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17933642, Length=255, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21355835, Length=254, Percent_Identity=27.9527559055118, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28086; Mature: 28086

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAAAIQFTIKPGNVDANLAHARDALGRASDRGAQLVVLPEMWSCGFCGKSLAGLAMHT
CEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
AAIAEELQDLSRRLGLVVVGSMPEPNGDRVFNTIHLIDNGRLAGIYRKVHLFSLLGEDRV
HHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEEEECCEEEHHHHHHHHHHHHCCCCE
FAAGDGCLLADTSIGKIGVIICYDLRFPELSRRLALEGAQILCIPAQWPKPRNEQWRTLL
EEECCCEEEECCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
QARAIENQFFVVASNTCGMVGKLDFFGMSMIIGPRGDVLAEAGDRETEIVAGLDMRDMAE
HHHHCCCEEEEEECCCCCCEECHHHHCEEEEECCCCCEEECCCCCCEEEEECCCHHHHHH
WRAQIPCFKDRRPELY
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MITAAAIQFTIKPGNVDANLAHARDALGRASDRGAQLVVLPEMWSCGFCGKSLAGLAMHT
CEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
AAIAEELQDLSRRLGLVVVGSMPEPNGDRVFNTIHLIDNGRLAGIYRKVHLFSLLGEDRV
HHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEEEECCEEEHHHHHHHHHHHHCCCCE
FAAGDGCLLADTSIGKIGVIICYDLRFPELSRRLALEGAQILCIPAQWPKPRNEQWRTLL
EEECCCEEEECCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
QARAIENQFFVVASNTCGMVGKLDFFGMSMIIGPRGDVLAEAGDRETEIVAGLDMRDMAE
HHHHCCCEEEEEECCCCCCEECHHHHCEEEEECCCCCEEECCCCCCEEEEECCCHHHHHH
WRAQIPCFKDRRPELY
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]