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The map label for this gene is tolB [H]

Identifier: 118581723

GI number: 118581723

Start: 3660616

End: 3661923

Strand: Reverse

Name: tolB [H]

Synonym: Ppro_3323

Alternate gene names: 118581723

Gene position: 3661923-3660616 (Counterclockwise)

Preceding gene: 118581724

Following gene: 118581722

Centisome position: 91.37

GC content: 60.17

Gene sequence:

>1308_bases
ATGAAAGTCGATACAATCACCATGATGAGAACCCTTCTGATCCTTCTCGTGCTGTTCCTGATGCTGCCGGCCGTGTCATG
GTCAGAGAACGGCTATATCGAGATAACCGCGCCGGGCAATCGCCAACTCAGGATGGCCGTGGATGTTCCCCGCAGTCTGG
AAGCGGCCTCGTCGCCCGAAAAGGCCCGGGAGATGGGGGAGGTGATCGCCTTCGACATGAACATGTCGGGCCTCTGTCTG
GCTGAAATCAAGGAACAGTTTAACCTCTCGGGCGCTCTGCTCTTTGCCGAGGCCGACTTCAACTCCTGGCTGAAGGGGGG
CTATGACCTGCTGGTGCGGGGCGAATACAGCGTAAAGGGAGACGATCTGACGGTCGAGTTCCGGCTCTTCGACCTGGCCA
ACCGCAAGATGATGACCGCCCGACGCTATCTGGGAAAGACAAAGGACCTGCGCAGGTTCGCCCACTCCTTTGCCGACGAG
ATCCTGCTGGCCATGACTGGTGAACGGGGCTGCTTTACCACCCATATTGCATTTGTCAGCACCAGATCCGGCAACAAGGA
GATCGCCATCATGGACTGGGACGGTCACGACACCCGGCAGCTGACCAGAAACCACTCCATCAACCTCAACCCCGACTTCT
CGCCCAACGGCCGCGAGATCATCTTCACCTCCTATAAGCGGCACAACCCGGATCTTTACCGGCGTCCCCTTTCCAGTCCG
GTGGAGGTGGCGGTCTCCCATCGCGAAGGTCTGAATATCACCGGCGCCTGGTCGCCCGATGGATCGCGAATCGCCCTGGC
TCTGAGCAAGGACGGAAATTCCGAAATCTATACCATTGCCCGGGACGGTTCCAGCCCGGTTCGCCTGACCTCTCACCCGG
CCATAGAAATTTCCCCCACCTGGTCGCCCAATGGCTCCCAGATCGCCTTCGTCTCCGACCGCCTGGGGAAACCGCAGATT
TTCATCATGAATGCCGATGGAACCGGCGTTCGGCGCCTTACCACCAACGGCGGCTACAACGTCAATCCGCGCTGGTCGCC
AAAGGGGGACAAAATCGCCTATGCTCGCATGGAGGGCGGTGGATTCCAGATCCATACCATCAATGTCAATGGTAGCGACG
ACACCCAGTTGACCAGCAGCGGCAGCAGCGAAAACCCGGCCTGGTCGCCCGATGGCCGCCTGATAGCGTTCAGCGGTAGG
AGGGGAAGTGGAGACGAAATCTACGTCATGCGGGCCGACGGCTCGGGAGAGACCCGCGTCAGCAAGGTCGGGGGGCGCAG
TACCCAGCCGGCCTGGCAGCCGCGCTGA

Upstream 100 bases:

>100_bases
CCATAGACAGGGCCAGTGAGAAGTTTCCCGCCCCTCCCGACAGGAGGGGTTTCGAGGGAGTATTTGTGTTCAAGCCCCAG
GGCGTTTCCAACAGCAGACC

Downstream 100 bases:

>100_bases
ACTCCTTCCAGGGGGGCGTTCGGTGGAGAACATTCGGCGTATTTTGAAAGAGGGTTGCAATTAGCAATTGCAAAAGAAAC
ATCCTGATGTATACTCTGCC

Product: WD40 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKVDTITMMRTLLILLVLFLMLPAVSWSENGYIEITAPGNRQLRMAVDVPRSLEAASSPEKAREMGEVIAFDMNMSGLCL
AEIKEQFNLSGALLFAEADFNSWLKGGYDLLVRGEYSVKGDDLTVEFRLFDLANRKMMTARRYLGKTKDLRRFAHSFADE
ILLAMTGERGCFTTHIAFVSTRSGNKEIAIMDWDGHDTRQLTRNHSINLNPDFSPNGREIIFTSYKRHNPDLYRRPLSSP
VEVAVSHREGLNITGAWSPDGSRIALALSKDGNSEIYTIARDGSSPVRLTSHPAIEISPTWSPNGSQIAFVSDRLGKPQI
FIMNADGTGVRRLTTNGGYNVNPRWSPKGDKIAYARMEGGGFQIHTINVNGSDDTQLTSSGSSENPAWSPDGRLIAFSGR
RGSGDEIYVMRADGSGETRVSKVGGRSTQPAWQPR

Sequences:

>Translated_435_residues
MKVDTITMMRTLLILLVLFLMLPAVSWSENGYIEITAPGNRQLRMAVDVPRSLEAASSPEKAREMGEVIAFDMNMSGLCL
AEIKEQFNLSGALLFAEADFNSWLKGGYDLLVRGEYSVKGDDLTVEFRLFDLANRKMMTARRYLGKTKDLRRFAHSFADE
ILLAMTGERGCFTTHIAFVSTRSGNKEIAIMDWDGHDTRQLTRNHSINLNPDFSPNGREIIFTSYKRHNPDLYRRPLSSP
VEVAVSHREGLNITGAWSPDGSRIALALSKDGNSEIYTIARDGSSPVRLTSHPAIEISPTWSPNGSQIAFVSDRLGKPQI
FIMNADGTGVRRLTTNGGYNVNPRWSPKGDKIAYARMEGGGFQIHTINVNGSDDTQLTSSGSSENPAWSPDGRLIAFSGR
RGSGDEIYVMRADGSGETRVSKVGGRSTQPAWQPR
>Mature_435_residues
MKVDTITMMRTLLILLVLFLMLPAVSWSENGYIEITAPGNRQLRMAVDVPRSLEAASSPEKAREMGEVIAFDMNMSGLCL
AEIKEQFNLSGALLFAEADFNSWLKGGYDLLVRGEYSVKGDDLTVEFRLFDLANRKMMTARRYLGKTKDLRRFAHSFADE
ILLAMTGERGCFTTHIAFVSTRSGNKEIAIMDWDGHDTRQLTRNHSINLNPDFSPNGREIIFTSYKRHNPDLYRRPLSSP
VEVAVSHREGLNITGAWSPDGSRIALALSKDGNSEIYTIARDGSSPVRLTSHPAIEISPTWSPNGSQIAFVSDRLGKPQI
FIMNADGTGVRRLTTNGGYNVNPRWSPKGDKIAYARMEGGGFQIHTINVNGSDDTQLTSSGSSENPAWSPDGRLIAFSGR
RGSGDEIYVMRADGSGETRVSKVGGRSTQPAWQPR

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=388, Percent_Identity=26.2886597938144, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48090; Mature: 48090

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDTITMMRTLLILLVLFLMLPAVSWSENGYIEITAPGNRQLRMAVDVPRSLEAASSPE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCHH
KAREMGEVIAFDMNMSGLCLAEIKEQFNLSGALLFAEADFNSWLKGGYDLLVRGEYSVKG
HHHHCCCEEEEECCCCCEEHHHHHHHHCCCEEEEEEECCHHHHHCCCEEEEEEECEEECC
DDLTVEFRLFDLANRKMMTARRYLGKTKDLRRFAHSFADEILLAMTGERGCFTTHIAFVS
CCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEE
TRSGNKEIAIMDWDGHDTRQLTRNHSINLNPDFSPNGREIIFTSYKRHNPDLYRRPLSSP
ECCCCCEEEEEECCCCCHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCHHHHCCCCCC
VEVAVSHREGLNITGAWSPDGSRIALALSKDGNSEIYTIARDGSSPVRLTSHPAIEISPT
CEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCEEEECCCEEEEECC
WSPNGSQIAFVSDRLGKPQIFIMNADGTGVRRLTTNGGYNVNPRWSPKGDKIAYARMEGG
CCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEECCCCEECCCCCCCCCCEEEEEEECCC
GFQIHTINVNGSDDTQLTSSGSSENPAWSPDGRLIAFSGRRGSGDEIYVMRADGSGETRV
CEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCEE
SKVGGRSTQPAWQPR
EECCCCCCCCCCCCC
>Mature Secondary Structure
MKVDTITMMRTLLILLVLFLMLPAVSWSENGYIEITAPGNRQLRMAVDVPRSLEAASSPE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCHH
KAREMGEVIAFDMNMSGLCLAEIKEQFNLSGALLFAEADFNSWLKGGYDLLVRGEYSVKG
HHHHCCCEEEEECCCCCEEHHHHHHHHCCCEEEEEEECCHHHHHCCCEEEEEEECEEECC
DDLTVEFRLFDLANRKMMTARRYLGKTKDLRRFAHSFADEILLAMTGERGCFTTHIAFVS
CCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEE
TRSGNKEIAIMDWDGHDTRQLTRNHSINLNPDFSPNGREIIFTSYKRHNPDLYRRPLSSP
ECCCCCEEEEEECCCCCHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCHHHHCCCCCC
VEVAVSHREGLNITGAWSPDGSRIALALSKDGNSEIYTIARDGSSPVRLTSHPAIEISPT
CEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCEEEECCCEEEEECC
WSPNGSQIAFVSDRLGKPQIFIMNADGTGVRRLTTNGGYNVNPRWSPKGDKIAYARMEGG
CCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEECCCCEECCCCCCCCCCEEEEEEECCC
GFQIHTINVNGSDDTQLTSSGSSENPAWSPDGRLIAFSGRRGSGDEIYVMRADGSGETRV
CEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCEE
SKVGGRSTQPAWQPR
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA