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The map label for this gene is cspLA [H]

Identifier: 118581708

GI number: 118581708

Start: 3646132

End: 3646332

Strand: Reverse

Name: cspLA [H]

Synonym: Ppro_3308

Alternate gene names: 118581708

Gene position: 3646332-3646132 (Counterclockwise)

Preceding gene: 118581709

Following gene: 118581707

Centisome position: 90.98

GC content: 47.76

Gene sequence:

>201_bases
ATGGTAAACGGAACAGTAAAATGGTTTAACGACAGCAAGGGGTTTGGATTTCTTGAGCAGGAAAATGGCGAAGACGTATT
CGTGCACTTTTCCGCGATTTCGGGCGAAGGCTTCAAATCCCTTGCAGAAGGTGATAGCGTTACGTTCGAGCTGGTCAAAG
GCCCCAAAGGTCTCCAGGCGGCAAACGTAACCAAGATTTAA

Upstream 100 bases:

>100_bases
TTAATTACATTGACAGCCCTGCGCCAGGCTGTTACTGTAACGAAGCTCTCGTGGATGCCACGGAGTAATTAAAAATAGCA
GTACCAGGAGAAGTAAGTAC

Downstream 100 bases:

>100_bases
TCCAAAGAAAAACGGAAACGCAAAAAAGCCTCGGAGAAATCCGGGGCTTTTTTGCGTACTGCGGGGATAGTGATTCCTGA
ACAGGAGCAGAGATGACGAA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAISGEGFKSLAEGDSVTFELVKGPKGLQAANVTKI

Sequences:

>Translated_66_residues
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAISGEGFKSLAEGDSVTFELVKGPKGLQAANVTKI
>Mature_66_residues
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAISGEGFKSLAEGDSVTFELVKGPKGLQAANVTKI

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=62.1212121212121, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=60.6060606060606, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=54.5454545454545, Blast_Score=72, Evalue=7e-15,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=47.7611940298507, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=53.968253968254, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=50, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=46.969696969697, Blast_Score=63, Evalue=3e-12,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7111; Mature: 7111

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAISGEGFKSLAEGDSVTFELVKGPKGLQA
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
ANVTKI
CCEECC
>Mature Secondary Structure
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAISGEGFKSLAEGDSVTFELVKGPKGLQA
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
ANVTKI
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]