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The map label for this gene is queF [H]

Identifier: 118581704

GI number: 118581704

Start: 3640667

End: 3641098

Strand: Reverse

Name: queF [H]

Synonym: Ppro_3304

Alternate gene names: 118581704

Gene position: 3641098-3640667 (Counterclockwise)

Preceding gene: 118581707

Following gene: 118581703

Centisome position: 90.85

GC content: 62.27

Gene sequence:

>432_bases
ATGACGCTGCACGAAAATCTGAAATCGCTTGGCTCGGGGACGACCGGCTACCGCTACGCCCGTCCCGATGCCACGCTACT
GGAGGCATTCCCCAGCCCTTTCGCCCAGCCCGATTTGAACCCGGCTGGCGCTGTGGGGACGCTGCACATCGAATGTCCCG
AGTTCACCTGTCTCTGTCCCATGACCGGCCAGCCTGATTTCGCCAGGATCGTGATCGACTACCAGCCCGATACGCTCTGC
GTGGAAAGCAAGAGCCTCAAGCTCTACCTTGGCTCATTCCGCATGCATGGCGAATTCCACGAGGCCAGTGTCAACCGCAT
CTGCAACGATCTGGTTCGGCTGATCTCCCCGCTCTGGCTGACGGTGCGCGGCGAGTTCACCCCCCGCGGCGGCATCCCCT
TCTGGCCTACGGCCGAGTACCGCAGGCCATAA

Upstream 100 bases:

>100_bases
CTGGGGCAGGGGCATCCTCTCGCCGGGAAAATAATGATTGCCAAGGGATTCGGAGCTATGCTATCGCAGATTTCAGGATC
GACCACAAGGGAGAACGCCC

Downstream 100 bases:

>100_bases
TTTTATTTCGTCATAGATTCAAGGAGTTTGCATGTACAGCACGTCTGATTTCAAGAAGGGGCTGGTGATCCAACTGGACG
GAGCCCCCTGTCTGATCGTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MTLHENLKSLGSGTTGYRYARPDATLLEAFPSPFAQPDLNPAGAVGTLHIECPEFTCLCPMTGQPDFARIVIDYQPDTLC
VESKSLKLYLGSFRMHGEFHEASVNRICNDLVRLISPLWLTVRGEFTPRGGIPFWPTAEYRRP

Sequences:

>Translated_143_residues
MTLHENLKSLGSGTTGYRYARPDATLLEAFPSPFAQPDLNPAGAVGTLHIECPEFTCLCPMTGQPDFARIVIDYQPDTLC
VESKSLKLYLGSFRMHGEFHEASVNRICNDLVRLISPLWLTVRGEFTPRGGIPFWPTAEYRRP
>Mature_142_residues
TLHENLKSLGSGTTGYRYARPDATLLEAFPSPFAQPDLNPAGAVGTLHIECPEFTCLCPMTGQPDFARIVIDYQPDTLCV
ESKSLKLYLGSFRMHGEFHEASVNRICNDLVRLISPLWLTVRGEFTPRGGIPFWPTAEYRRP

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=78, Percent_Identity=42.3076923076923, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 16002; Mature: 15870

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHENLKSLGSGTTGYRYARPDATLLEAFPSPFAQPDLNPAGAVGTLHIECPEFTCLCP
CCHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCEEEECC
MTGQPDFARIVIDYQPDTLCVESKSLKLYLGSFRMHGEFHEASVNRICNDLVRLISPLWL
CCCCCCEEEEEEEECCCEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEE
TVRGEFTPRGGIPFWPTAEYRRP
EEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLHENLKSLGSGTTGYRYARPDATLLEAFPSPFAQPDLNPAGAVGTLHIECPEFTCLCP
CHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCEEEECC
MTGQPDFARIVIDYQPDTLCVESKSLKLYLGSFRMHGEFHEASVNRICNDLVRLISPLWL
CCCCCCEEEEEEEECCCEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEE
TVRGEFTPRGGIPFWPTAEYRRP
EEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA