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The map label for this gene is mraW

Identifier: 118581697

GI number: 118581697

Start: 3634941

End: 3635882

Strand: Reverse

Name: mraW

Synonym: Ppro_3297

Alternate gene names: 118581697

Gene position: 3635882-3634941 (Counterclockwise)

Preceding gene: 118581698

Following gene: 118581696

Centisome position: 90.72

GC content: 64.97

Gene sequence:

>942_bases
ATGGCCGGCTTCCAGCATCTCTCGGTCATGCCGGAGGAAGTGCTACGCTTCCTCTCCCCCCGCCCCGGCGGCTGCTATCT
GGACGGAACCCTGGGTGGCGGCGGGCACGCGGCACTGGTCGCCGAGCGCTGCGCTCCGGGCGGCGGAACCCTGATCGGCA
TGGATCGGGACCTGGAGGCTTTGAAGGCGGCCGCTGAGCGCCTCTCCCGCTTCGGAGATGCTGTCCGGCTGTTGCATGGC
AACTTCGCGGACATCGCCACAAGGCTGGACTCCCTGGGAGTTGACGCCCTTGACGGCTTTGTCCTCGATCTTGGGGTCTC
CTCGCACCAGCTTGACACGGCCCGACGCGGCTTCAGCTTCCAGCAGGAGGGGGCGCTGGACATGCGCATGGATGGCGACA
GCGGAGAAACGGCTGCTGACCTGGTGAACCGCCTGCCGGAGCATGAGCTGGAACGGATCATCAGGGAGTACGGCGAAGAA
CGCTGGGCAAAACGGATAGCCTCCTTCATCGTCAGGGCGCGCAGCGAGGCCCCCATTGAGAACACCCTGCGCCTGGTGGA
CATCATCAAGGGAGCCATTCCCAAGGCAAAGTGGGAAGAGCGTCTGCACCCCGCCACCCGCACCTTCCAGGCGTTGAGGA
TAGCGGTCAACCATGAGCTGGAGAGCCTTGAACGGGGGCTTAAAAGCGCCATCGATCGCCTCAAACCGGGGGGGCGGGGG
GTGGTGATCTCCTTCCACTCCCTGGAGGATCGCATTGTCAAGCATGTCTTCCGTGAGTATGCCGCCGGCTGCACCTGCCC
CCGCAACCTGCCGGTCTGTGTCTGCGGCAGGCAACCCCGGGTGCGGGTTCTGACGGGAAGGCCGGTAACGGCTACGGAGG
AGGAACTCCGGGACAATCCACGTTCGCGCAGCGCAAAGCTCCGCGCGGTGGAAAAACTGTAG

Upstream 100 bases:

>100_bases
GGACGGGCGCTTCGATATCTGGAGCAGGGACACCTATGACAGAGTCAATGACCAGGATGAGCAGAACCTTCCTGCGGATT
TGACGGCGATCGGTTTCTAG

Downstream 100 bases:

>100_bases
TGAAGCTGGAAACGGTCATCAGTCGCAGGGAGGGTAGAGATGTCACAGGCAAAAACCGAGTACGGCAAGGTAGCCGCGCC
GCGCGCCATCGGCGGAGCGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAGFQHLSVMPEEVLRFLSPRPGGCYLDGTLGGGGHAALVAERCAPGGGTLIGMDRDLEALKAAAERLSRFGDAVRLLHG
NFADIATRLDSLGVDALDGFVLDLGVSSHQLDTARRGFSFQQEGALDMRMDGDSGETAADLVNRLPEHELERIIREYGEE
RWAKRIASFIVRARSEAPIENTLRLVDIIKGAIPKAKWEERLHPATRTFQALRIAVNHELESLERGLKSAIDRLKPGGRG
VVISFHSLEDRIVKHVFREYAAGCTCPRNLPVCVCGRQPRVRVLTGRPVTATEEELRDNPRSRSAKLRAVEKL

Sequences:

>Translated_313_residues
MAGFQHLSVMPEEVLRFLSPRPGGCYLDGTLGGGGHAALVAERCAPGGGTLIGMDRDLEALKAAAERLSRFGDAVRLLHG
NFADIATRLDSLGVDALDGFVLDLGVSSHQLDTARRGFSFQQEGALDMRMDGDSGETAADLVNRLPEHELERIIREYGEE
RWAKRIASFIVRARSEAPIENTLRLVDIIKGAIPKAKWEERLHPATRTFQALRIAVNHELESLERGLKSAIDRLKPGGRG
VVISFHSLEDRIVKHVFREYAAGCTCPRNLPVCVCGRQPRVRVLTGRPVTATEEELRDNPRSRSAKLRAVEKL
>Mature_312_residues
AGFQHLSVMPEEVLRFLSPRPGGCYLDGTLGGGGHAALVAERCAPGGGTLIGMDRDLEALKAAAERLSRFGDAVRLLHGN
FADIATRLDSLGVDALDGFVLDLGVSSHQLDTARRGFSFQQEGALDMRMDGDSGETAADLVNRLPEHELERIIREYGEER
WAKRIASFIVRARSEAPIENTLRLVDIIKGAIPKAKWEERLHPATRTFQALRIAVNHELESLERGLKSAIDRLKPGGRGV
VISFHSLEDRIVKHVFREYAAGCTCPRNLPVCVCGRQPRVRVLTGRPVTATEEELRDNPRSRSAKLRAVEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=42.3529411764706, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI165377202, Length=210, Percent_Identity=41.9047619047619, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786270, Length=324, Percent_Identity=42.9012345679012, Blast_Score=229, Evalue=1e-61,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=38.368580060423, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=38.368580060423, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PELPD (A1AU69)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902947.1
- ProteinModelPortal:   A1AU69
- SMR:   A1AU69
- STRING:   A1AU69
- GeneID:   4572586
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3297
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   A1AU69
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34487; Mature: 34356

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 82-82 BINDING 103-103 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFQHLSVMPEEVLRFLSPRPGGCYLDGTLGGGGHAALVAERCAPGGGTLIGMDRDLEA
CCCCHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCHHH
LKAAAERLSRFGDAVRLLHGNFADIATRLDSLGVDALDGFVLDLGVSSHQLDTARRGFSF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCEECCCCHHHHHHHHHCCCC
QQEGALDMRMDGDSGETAADLVNRLPEHELERIIREYGEERWAKRIASFIVRARSEAPIE
CCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
NTLRLVDIIKGAIPKAKWEERLHPATRTFQALRIAVNHELESLERGLKSAIDRLKPGGRG
HHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVISFHSLEDRIVKHVFREYAAGCTCPRNLPVCVCGRQPRVRVLTGRPVTATEEELRDNP
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHCCC
RSRSAKLRAVEKL
CHHHHHHHHHHCC
>Mature Secondary Structure 
AGFQHLSVMPEEVLRFLSPRPGGCYLDGTLGGGGHAALVAERCAPGGGTLIGMDRDLEA
CCCHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCHHH
LKAAAERLSRFGDAVRLLHGNFADIATRLDSLGVDALDGFVLDLGVSSHQLDTARRGFSF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCEECCCCHHHHHHHHHCCCC
QQEGALDMRMDGDSGETAADLVNRLPEHELERIIREYGEERWAKRIASFIVRARSEAPIE
CCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
NTLRLVDIIKGAIPKAKWEERLHPATRTFQALRIAVNHELESLERGLKSAIDRLKPGGRG
HHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVISFHSLEDRIVKHVFREYAAGCTCPRNLPVCVCGRQPRVRVLTGRPVTATEEELRDNP
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHCCC
RSRSAKLRAVEKL
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA