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The map label for this gene is ddl

Identifier: 118581686

GI number: 118581686

Start: 3621500

End: 3622432

Strand: Reverse

Name: ddl

Synonym: Ppro_3286

Alternate gene names: 118581686

Gene position: 3622432-3621500 (Counterclockwise)

Preceding gene: 118581687

Following gene: 118581685

Centisome position: 90.38

GC content: 64.74

Gene sequence:

>933_bases
ATGAATGACATCAGATCCAGAAAGATTGCCGTCCTCATGGGCGGGCTCTCGGCCGAGCGGGAAGTCTCCCTAGCCAGCGG
AGCCGCCGTGTGCCAGGCACTTGTGGCCAGGGGGTTCGACGCCCTTAGCGTGGACGTGGCCAGGGACCTCCCCCTGGTCC
TGAGCCGCGAAGGCATCGGCGCGGCCTTCATCGCCCTGCACGGGCGCTATGGCGAGGATGGCTGCGTTCAGGGGTTGCTG
GAGCTGATGGCTATCCCCTACACCGGTTCCGGCGTGCTGGCCAGCGCCCTGGCCATGCACAAGCTCTACAGCAAGCAGGC
CTTCGTCAGTGCCGGCATCCTCACCGCCCCCTTCCACCATTTCCGGCGCGGGGAGCGGGTCTCCCTCAGCCACCTCAGCT
TCGGCCTGCCGCTGGTGGTCAAGCCGGTGCAGGAAGGGTCTTCGGTGGGTATCTCCATCGTCAAGGAGGAGAGCCAGCTG
GCTGCTGCCGTGAAGCTGGCCTTCCGTCATGACGACGAGATCCTGGTGGAACAGTTCATCAAGGGGCAGGAGGTGCAGGT
CGGCATCCTGGACGACCGCCCCATGGGTGCCATCGAGATCGTGTCCAGGAACGAATTCTATGATTTTGAGGCCAAGTACA
CCGATGGCATGGCCGAGCACTTCTTTCCCGCCCGCCTGGAGAAGGGGCTCTACGAAGAGGCGCTGCGGGTCGGCCTGGCC
GCCCACCATGCCCTGGGATGCCGCTGCTACAGCCGGGTCGACCTGCTGGTGACTCCCGCAGGGGAGTGCTACGTGCTGGA
GGTGAACACCCTGCCCGGTATGACGGCGCTCAGTCTGCTGCCGGAGATCGCGGCCAAGGGAGCCGATCTCCCCTTCGAGG
AACTGGTGGAACGGATCATCCTGTCTGCCGACCTGTCGGTGAAAACGGGGTAA

Upstream 100 bases:

>100_bases
GATTTCCTGGAACTTTCACGGGTTGTGAAAAAAGCGGTTCTGGCCTCTTGCGGGGTGACCCTGGAGGAGGAAGTGCGTAT
AGTTGGCGAGGAGTAACGTT

Downstream 100 bases:

>100_bases
AGCCATGCGGGATTATGCCTCCCCACGATACCAGCGCAAACAGTCATCCTCCAACCGGGCCAAGAAGCAGCGCAAGCCGC
TGAACCTGAAAAAATACCTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNDIRSRKIAVLMGGLSAEREVSLASGAAVCQALVARGFDALSVDVARDLPLVLSREGIGAAFIALHGRYGEDGCVQGLL
ELMAIPYTGSGVLASALAMHKLYSKQAFVSAGILTAPFHHFRRGERVSLSHLSFGLPLVVKPVQEGSSVGISIVKEESQL
AAAVKLAFRHDDEILVEQFIKGQEVQVGILDDRPMGAIEIVSRNEFYDFEAKYTDGMAEHFFPARLEKGLYEEALRVGLA
AHHALGCRCYSRVDLLVTPAGECYVLEVNTLPGMTALSLLPEIAAKGADLPFEELVERIILSADLSVKTG

Sequences:

>Translated_310_residues
MNDIRSRKIAVLMGGLSAEREVSLASGAAVCQALVARGFDALSVDVARDLPLVLSREGIGAAFIALHGRYGEDGCVQGLL
ELMAIPYTGSGVLASALAMHKLYSKQAFVSAGILTAPFHHFRRGERVSLSHLSFGLPLVVKPVQEGSSVGISIVKEESQL
AAAVKLAFRHDDEILVEQFIKGQEVQVGILDDRPMGAIEIVSRNEFYDFEAKYTDGMAEHFFPARLEKGLYEEALRVGLA
AHHALGCRCYSRVDLLVTPAGECYVLEVNTLPGMTALSLLPEIAAKGADLPFEELVERIILSADLSVKTG
>Mature_310_residues
MNDIRSRKIAVLMGGLSAEREVSLASGAAVCQALVARGFDALSVDVARDLPLVLSREGIGAAFIALHGRYGEDGCVQGLL
ELMAIPYTGSGVLASALAMHKLYSKQAFVSAGILTAPFHHFRRGERVSLSHLSFGLPLVVKPVQEGSSVGISIVKEESQL
AAAVKLAFRHDDEILVEQFIKGQEVQVGILDDRPMGAIEIVSRNEFYDFEAKYTDGMAEHFFPARLEKGLYEEALRVGLA
AHHALGCRCYSRVDLLVTPAGECYVLEVNTLPGMTALSLLPEIAAKGADLPFEELVERIILSADLSVKTG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=46.7320261437908, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1786579, Length=344, Percent_Identity=32.8488372093023, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PELPD (A1AU58)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902936.1
- ProteinModelPortal:   A1AU58
- SMR:   A1AU58
- STRING:   A1AU58
- GeneID:   4572280
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3286
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   A1AU58
- ProtClustDB:   CLSK829085
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33365; Mature: 33365

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIRSRKIAVLMGGLSAEREVSLASGAAVCQALVARGFDALSVDVARDLPLVLSREGIG
CCCCHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCEEECCCCC
AAFIALHGRYGEDGCVQGLLELMAIPYTGSGVLASALAMHKLYSKQAFVSAGILTAPFHH
EEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FRRGERVSLSHLSFGLPLVVKPVQEGSSVGISIVKEESQLAAAVKLAFRHDDEILVEQFI
HCCCCEEEEHHHCCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHH
KGQEVQVGILDDRPMGAIEIVSRNEFYDFEAKYTDGMAEHFFPARLEKGLYEEALRVGLA
CCCCEEEEEECCCCCCEEEEEECCCCEEECCHHCCCHHHHCCHHHHHHHHHHHHHHHHHH
AHHALGCRCYSRVDLLVTPAGECYVLEVNTLPGMTALSLLPEIAAKGADLPFEELVERII
HHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
LSADLSVKTG
HHCCCEECCC
>Mature Secondary Structure
MNDIRSRKIAVLMGGLSAEREVSLASGAAVCQALVARGFDALSVDVARDLPLVLSREGIG
CCCCHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCEEECCCCC
AAFIALHGRYGEDGCVQGLLELMAIPYTGSGVLASALAMHKLYSKQAFVSAGILTAPFHH
EEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FRRGERVSLSHLSFGLPLVVKPVQEGSSVGISIVKEESQLAAAVKLAFRHDDEILVEQFI
HCCCCEEEEHHHCCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHH
KGQEVQVGILDDRPMGAIEIVSRNEFYDFEAKYTDGMAEHFFPARLEKGLYEEALRVGLA
CCCCEEEEEECCCCCCEEEEEECCCCEEECCHHCCCHHHHCCHHHHHHHHHHHHHHHHHH
AHHALGCRCYSRVDLLVTPAGECYVLEVNTLPGMTALSLLPEIAAKGADLPFEELVERII
HHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
LSADLSVKTG
HHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA