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The map label for this gene is otsA [H]

Identifier: 118581671

GI number: 118581671

Start: 3603369

End: 3605612

Strand: Reverse

Name: otsA [H]

Synonym: Ppro_3271

Alternate gene names: 118581671

Gene position: 3605612-3603369 (Counterclockwise)

Preceding gene: 118581672

Following gene: 118581670

Centisome position: 89.96

GC content: 59.54

Gene sequence:

>2244_bases
ATGAAGCCTCTTCGTTCACTTCGCTTGTCTTTGCGTTTCATTATTCCCCTCGTCGTGGCCCTGACCCTGTTGGCCTTTGC
GGTGGTGCCGTTGGTGGAAAAGCTGTCTCTGCGCTGGTTTGTCAGGGACCTGGATATCCGTTCCCGCATGATCGCCAGCA
GCATGCGGGAACCGCTGGAGGAGTTCCTGGCAACGGAAAACAGGGCCAAGATCAGGGCGCTGTTCCTGCGCGCCGCTCAG
GATGAACGTCTGTTGGCCATCGGGTATTGCGATGAAGATGGGAAGCTTGTCTATCGTACCCCCAACTTCCCCGCCGCCCG
CGCCTGTCCCCCTCCCCATCCGTCACTACGAGAATCCACGATCGTGTCGAATCTGCCCCAAGGGGCGGTTCATATCTCAG
CCCATGCCCTGTTCCTGGAGGGAAAACTTTCCGGATGGCTGGTGCTGGTTCATGACATGAGCTTTGTCGAGCACCGCAGC
GCCGAGACCCGCAAGTATATCATCGCCCTGTTTGCCGCACTCGGTGTGGTCATCTCACTGATCACGGTATTCGTGGCCCA
TCTGAGCTGGAGGGGCTGGATGGAAGGGGTTCGGGCCATGCTGCGCGGCGAGGGGATCCTGCGCCCGTTCGGGCAGCAGC
ACGTCCCGGATCCGGAACTGCAGCCTCTGGTGGGAGACCTGCGGGCACTGCTGCGGAGCATGGATGTCGAGCGCCGTTTC
ACAGATGACTCCACCATTACCTGGAGCCCCGATTCCCTGCGCAGTTTGTTGCATAAGCAGTTGGCCGGAGAGCGGATCAT
CGTGGTTTCCAATCGGGAACCCTATATCCACCTCAAAAAAGAGGACCGCATCGAAGTTAACCGTCCCGCCAGTGGTCTGG
TAACCGCTGTCGAACCGGTCATGCGCGCCTGCTCCGGGACCTGGATTGCCCACGGTAGCGGCAGTGCCGACCGGGAGACC
GTCGACCGTCATGACCGGGTGGCCGTCCCGCCCGATCATCCCGAATACCGGCTTCGCCGCATCTGGCTGACCACGGAAGA
GGAAAACGGTTACTACTACGGTTTTGCCAATGAGGGGCTCTGGCCGCTCTGTCATATCGCCCATGTGCGCCCCATTTTCC
GTACCAGCGACTGGCAGTCGTACGTGGCCATCAACCAGCGCTTCGCCGATGCTGTGGTTCGGGAGGCGGACAGCGACGAT
CCGGTGGTACTGGTGCAGGATTACCACTTCGCTCTGCTACCGGGCATGGTCCGCGCGGCACTGCCCAAGGCTACCATAAT
CACCTTCTGGCACATTCCCTGGCCCAACCCGGAGTCGTTCGGTATTTGCCCCTGGCGGGAAGAGCTGCTGCGGGGGATGC
TGGGAAGCACTATCCTCGGTTTCCACACCCCCTTCCATTGCAAGAATTTCCTTGAGACCGTTGATCGTTATCTGGAAACC
CGTATCGAACATGAATCATCGACCATCTCGCGACGTGGCAATCTGACCCTGGTGGAAAGCTATCCTATCTCGATCCAGTG
GCCGCCCGCCTGGGAGAAAAATCTGCCTTCCGTCGAGCAGTGCCGAAGCGAAGTGCGTGCGGCATTGGGACTTCCCGTGC
ACCACAGAATAGGCATCGGGGTGGATCGCCAGGACTACACCAAGGGAATCCTGGAACGGTTCCGGGCCGTGGAGAAAATG
CTGGAACTGCATCCGGAACTGGTGGGCAACTTCACCCTTATTCAGATCGCCGCCCCAACCCGCTCGGCGCTTGAGGATTA
CCAGACATTCGAAGCACAGGTCCGCAACCTGGCCATCCGGATCAATCAGCGTTTTTCCCGTGGAAACTGGCAGCCTATCT
GCCTCAAGGTCGAACATCATGAACCTGAACAGGTCAATCGATACTACCGTGCGGCGGATGTCTGTGTGGTGACCAGCCTG
CACGATGGCATGAATCTGGTGGCAAAGGAGTTCGTGGCGGCCAGAGAGGATGAGCGGGGAGTGCTGGTTTTGAGTCAGTT
CACCGGGGCGGCTCATGAGTTGCACGAATCCCTGATTGTCAACCCCTACCATATCGAACAGACCGCTGATGCCATCCATC
GGGCGCTTACCATGCCGGAAGAGGAACAGCGCGAGCGGATGCACAGCATGCGCATGCTGGTGCGCGACTTCAACGTCTAC
CGCTGGGCCGGCAGGATGCTGCTGGACGCTGCCCGCATCCGGCAGCGGGAAAAACTGGCGGCGCGCATTGGAGAAAGATC
ATGA

Upstream 100 bases:

>100_bases
ATGATGCATATGCGTTCCGACGAACGACCTCCGAGCCGTGCTCCGCCCCTCCGGGGGGAGCACGGCACCGGGTATGAACC
TCTGCTGCAAAGGAGTATGC

Downstream 100 bases:

>100_bases
TCCAGAACAGACCGGGACGCTACCTGCTGGACGATGCCGGATTGACGGAGCTTAGGTCATTCGTTGATGCAGGCACGCTC
CTGGCTTTTGACCTTGATGG

Product: alpha,alpha-trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MKPLRSLRLSLRFIIPLVVALTLLAFAVVPLVEKLSLRWFVRDLDIRSRMIASSMREPLEEFLATENRAKIRALFLRAAQ
DERLLAIGYCDEDGKLVYRTPNFPAARACPPPHPSLRESTIVSNLPQGAVHISAHALFLEGKLSGWLVLVHDMSFVEHRS
AETRKYIIALFAALGVVISLITVFVAHLSWRGWMEGVRAMLRGEGILRPFGQQHVPDPELQPLVGDLRALLRSMDVERRF
TDDSTITWSPDSLRSLLHKQLAGERIIVVSNREPYIHLKKEDRIEVNRPASGLVTAVEPVMRACSGTWIAHGSGSADRET
VDRHDRVAVPPDHPEYRLRRIWLTTEEENGYYYGFANEGLWPLCHIAHVRPIFRTSDWQSYVAINQRFADAVVREADSDD
PVVLVQDYHFALLPGMVRAALPKATIITFWHIPWPNPESFGICPWREELLRGMLGSTILGFHTPFHCKNFLETVDRYLET
RIEHESSTISRRGNLTLVESYPISIQWPPAWEKNLPSVEQCRSEVRAALGLPVHHRIGIGVDRQDYTKGILERFRAVEKM
LELHPELVGNFTLIQIAAPTRSALEDYQTFEAQVRNLAIRINQRFSRGNWQPICLKVEHHEPEQVNRYYRAADVCVVTSL
HDGMNLVAKEFVAAREDERGVLVLSQFTGAAHELHESLIVNPYHIEQTADAIHRALTMPEEEQRERMHSMRMLVRDFNVY
RWAGRMLLDAARIRQREKLAARIGERS

Sequences:

>Translated_747_residues
MKPLRSLRLSLRFIIPLVVALTLLAFAVVPLVEKLSLRWFVRDLDIRSRMIASSMREPLEEFLATENRAKIRALFLRAAQ
DERLLAIGYCDEDGKLVYRTPNFPAARACPPPHPSLRESTIVSNLPQGAVHISAHALFLEGKLSGWLVLVHDMSFVEHRS
AETRKYIIALFAALGVVISLITVFVAHLSWRGWMEGVRAMLRGEGILRPFGQQHVPDPELQPLVGDLRALLRSMDVERRF
TDDSTITWSPDSLRSLLHKQLAGERIIVVSNREPYIHLKKEDRIEVNRPASGLVTAVEPVMRACSGTWIAHGSGSADRET
VDRHDRVAVPPDHPEYRLRRIWLTTEEENGYYYGFANEGLWPLCHIAHVRPIFRTSDWQSYVAINQRFADAVVREADSDD
PVVLVQDYHFALLPGMVRAALPKATIITFWHIPWPNPESFGICPWREELLRGMLGSTILGFHTPFHCKNFLETVDRYLET
RIEHESSTISRRGNLTLVESYPISIQWPPAWEKNLPSVEQCRSEVRAALGLPVHHRIGIGVDRQDYTKGILERFRAVEKM
LELHPELVGNFTLIQIAAPTRSALEDYQTFEAQVRNLAIRINQRFSRGNWQPICLKVEHHEPEQVNRYYRAADVCVVTSL
HDGMNLVAKEFVAAREDERGVLVLSQFTGAAHELHESLIVNPYHIEQTADAIHRALTMPEEEQRERMHSMRMLVRDFNVY
RWAGRMLLDAARIRQREKLAARIGERS
>Mature_747_residues
MKPLRSLRLSLRFIIPLVVALTLLAFAVVPLVEKLSLRWFVRDLDIRSRMIASSMREPLEEFLATENRAKIRALFLRAAQ
DERLLAIGYCDEDGKLVYRTPNFPAARACPPPHPSLRESTIVSNLPQGAVHISAHALFLEGKLSGWLVLVHDMSFVEHRS
AETRKYIIALFAALGVVISLITVFVAHLSWRGWMEGVRAMLRGEGILRPFGQQHVPDPELQPLVGDLRALLRSMDVERRF
TDDSTITWSPDSLRSLLHKQLAGERIIVVSNREPYIHLKKEDRIEVNRPASGLVTAVEPVMRACSGTWIAHGSGSADRET
VDRHDRVAVPPDHPEYRLRRIWLTTEEENGYYYGFANEGLWPLCHIAHVRPIFRTSDWQSYVAINQRFADAVVREADSDD
PVVLVQDYHFALLPGMVRAALPKATIITFWHIPWPNPESFGICPWREELLRGMLGSTILGFHTPFHCKNFLETVDRYLET
RIEHESSTISRRGNLTLVESYPISIQWPPAWEKNLPSVEQCRSEVRAALGLPVHHRIGIGVDRQDYTKGILERFRAVEKM
LELHPELVGNFTLIQIAAPTRSALEDYQTFEAQVRNLAIRINQRFSRGNWQPICLKVEHHEPEQVNRYYRAADVCVVTSL
HDGMNLVAKEFVAAREDERGVLVLSQFTGAAHELHESLIVNPYHIEQTADAIHRALTMPEEEQRERMHSMRMLVRDFNVY
RWAGRMLLDAARIRQREKLAARIGERS

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=497, Percent_Identity=31.58953722334, Blast_Score=203, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71987012, Length=569, Percent_Identity=26.1862917398945, Blast_Score=149, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI115533560, Length=525, Percent_Identity=26.2857142857143, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI115533558, Length=525, Percent_Identity=26.2857142857143, Blast_Score=142, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319602, Length=474, Percent_Identity=31.0126582278481, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6323537, Length=385, Percent_Identity=29.8701298701299, Blast_Score=157, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6323917, Length=385, Percent_Identity=28.051948051948, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320279, Length=403, Percent_Identity=27.2952853598015, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19920676, Length=506, Percent_Identity=33.7944664031621, Blast_Score=267, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 85549; Mature: 85549

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLRSLRLSLRFIIPLVVALTLLAFAVVPLVEKLSLRWFVRDLDIRSRMIASSMREPLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLATENRAKIRALFLRAAQDERLLAIGYCDEDGKLVYRTPNFPAARACPPPHPSLREST
HHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHH
IVSNLPQGAVHISAHALFLEGKLSGWLVLVHDMSFVEHRSAETRKYIIALFAALGVVISL
HHHCCCCCCEEEEEEEEEEECCCCCEEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ITVFVAHLSWRGWMEGVRAMLRGEGILRPFGQQHVPDPELQPLVGDLRALLRSMDVERRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHC
TDDSTITWSPDSLRSLLHKQLAGERIIVVSNREPYIHLKKEDRIEVNRPASGLVTAVEPV
CCCCEEEECHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEECCCCCHHHHHHHHH
MRACSGTWIAHGSGSADRETVDRHDRVAVPPDHPEYRLRRIWLTTEEENGYYYGFANEGL
HHHHCCCEEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCC
WPLCHIAHVRPIFRTSDWQSYVAINQRFADAVVREADSDDPVVLVQDYHFALLPGMVRAA
CHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
LPKATIITFWHIPWPNPESFGICPWREELLRGMLGSTILGFHTPFHCKNFLETVDRYLET
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHH
RIEHESSTISRRGNLTLVESYPISIQWPPAWEKNLPSVEQCRSEVRAALGLPVHHRIGIG
HHHHCHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCC
VDRQDYTKGILERFRAVEKMLELHPELVGNFTLIQIAAPTRSALEDYQTFEAQVRNLAIR
CCHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHE
INQRFSRGNWQPICLKVEHHEPEQVNRYYRAADVCVVTSLHDGMNLVAKEFVAAREDERG
EECHHCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEEEHHCCHHHHHHHHHHHCCCCCC
VLVLSQFTGAAHELHESLIVNPYHIEQTADAIHRALTMPEEEQRERMHSMRMLVRDFNVY
EEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RWAGRMLLDAARIRQREKLAARIGERS
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPLRSLRLSLRFIIPLVVALTLLAFAVVPLVEKLSLRWFVRDLDIRSRMIASSMREPLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLATENRAKIRALFLRAAQDERLLAIGYCDEDGKLVYRTPNFPAARACPPPHPSLREST
HHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHH
IVSNLPQGAVHISAHALFLEGKLSGWLVLVHDMSFVEHRSAETRKYIIALFAALGVVISL
HHHCCCCCCEEEEEEEEEEECCCCCEEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ITVFVAHLSWRGWMEGVRAMLRGEGILRPFGQQHVPDPELQPLVGDLRALLRSMDVERRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHC
TDDSTITWSPDSLRSLLHKQLAGERIIVVSNREPYIHLKKEDRIEVNRPASGLVTAVEPV
CCCCEEEECHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEECCCCCHHHHHHHHH
MRACSGTWIAHGSGSADRETVDRHDRVAVPPDHPEYRLRRIWLTTEEENGYYYGFANEGL
HHHHCCCEEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCC
WPLCHIAHVRPIFRTSDWQSYVAINQRFADAVVREADSDDPVVLVQDYHFALLPGMVRAA
CHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
LPKATIITFWHIPWPNPESFGICPWREELLRGMLGSTILGFHTPFHCKNFLETVDRYLET
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHH
RIEHESSTISRRGNLTLVESYPISIQWPPAWEKNLPSVEQCRSEVRAALGLPVHHRIGIG
HHHHCHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCC
VDRQDYTKGILERFRAVEKMLELHPELVGNFTLIQIAAPTRSALEDYQTFEAQVRNLAIR
CCHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHE
INQRFSRGNWQPICLKVEHHEPEQVNRYYRAADVCVVTSLHDGMNLVAKEFVAAREDERG
EECHHCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEEEHHCCHHHHHHHHHHHCCCCCC
VLVLSQFTGAAHELHESLIVNPYHIEQTADAIHRALTMPEEEQRERMHSMRMLVRDFNVY
EEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RWAGRMLLDAARIRQREKLAARIGERS
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA