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The map label for this gene is otsB [H]

Identifier: 118581670

GI number: 118581670

Start: 3602581

End: 3603372

Strand: Reverse

Name: otsB [H]

Synonym: Ppro_3270

Alternate gene names: 118581670

Gene position: 3603372-3602581 (Counterclockwise)

Preceding gene: 118581671

Following gene: 118581669

Centisome position: 89.9

GC content: 60.86

Gene sequence:

>792_bases
ATGATCCAGAACAGACCGGGACGCTACCTGCTGGACGATGCCGGATTGACGGAGCTTAGGTCATTCGTTGATGCAGGCAC
GCTCCTGGCTTTTGACCTTGATGGAACCCTGGCGCCAATCGTGGCCGACCCGTCGGCCATCGTGATTCCCGGCCATATCC
AGGAGAGTATGGCCCGCCTTTGTGCCATGGCAACTGTCGTCATACTGACCGGCCGTGGCCGTGAGGATGCCTTATCCTTT
CTCGGCTTTGAGCCGTGCATGGTTGTGGGCAATCACGGCGCGGAGGGACTGCCCGGATGGGAGGAACGCGAACGGGAGTT
CAGGGAGCTCTGCGCCGGCTGGAAGCAACAGCTTCACCTGTCTCTGGCCGGCACTTCCAGCGGCATTTTCCTGGAAGACA
AGCGACTGTCGCTGTCACTCCATTATCGCAACGCAGCAGGCCGGGGTGAGGCCTTTGCGACGATCATAGAGGCGATCGGG
GCACTGGTGCCCGCTCCGGAGCGGATTTCAGGCAAGTTCGTGGAGAACGTCGTACCGCGGGGTGCGCCTCGCAAGGGGCG
TGCCCTGTTGGACACCCTGCGCTATCTGGGTACGGGGCGGGCGTTGTATGTAGGAGACGACGCCACGGACGAGGACGTGT
TCCGCCTGCGCAACGAGCGAATATTCGGAGTCAGGGTGGGTGCGAGTTCCTCGACAGCGGCAGACTATTATCTGAAAGAC
CAGCAGGAAATCGACCGTGTGCTCCGTGAGATCATTGACATTCTCTCGCATCCTTCCTGCGATGCGGCCTAG

Upstream 100 bases:

>100_bases
TGCGCGACTTCAACGTCTACCGCTGGGCCGGCAGGATGCTGCTGGACGCTGCCCGCATCCGGCAGCGGGAAAAACTGGCG
GCGCGCATTGGAGAAAGATC

Downstream 100 bases:

>100_bases
GCCCTGCCCCATCCTCTCCTTCGACGAAGCAGCTATGCTTTCCGCTAAACTCTGGCACAATTGAAGAGATCCACCTGTCC
GGCCGCTTCGGCAAGGCTTC

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MIQNRPGRYLLDDAGLTELRSFVDAGTLLAFDLDGTLAPIVADPSAIVIPGHIQESMARLCAMATVVILTGRGREDALSF
LGFEPCMVVGNHGAEGLPGWEEREREFRELCAGWKQQLHLSLAGTSSGIFLEDKRLSLSLHYRNAAGRGEAFATIIEAIG
ALVPAPERISGKFVENVVPRGAPRKGRALLDTLRYLGTGRALYVGDDATDEDVFRLRNERIFGVRVGASSSTAADYYLKD
QQEIDRVLREIIDILSHPSCDAA

Sequences:

>Translated_263_residues
MIQNRPGRYLLDDAGLTELRSFVDAGTLLAFDLDGTLAPIVADPSAIVIPGHIQESMARLCAMATVVILTGRGREDALSF
LGFEPCMVVGNHGAEGLPGWEEREREFRELCAGWKQQLHLSLAGTSSGIFLEDKRLSLSLHYRNAAGRGEAFATIIEAIG
ALVPAPERISGKFVENVVPRGAPRKGRALLDTLRYLGTGRALYVGDDATDEDVFRLRNERIFGVRVGASSSTAADYYLKD
QQEIDRVLREIIDILSHPSCDAA
>Mature_263_residues
MIQNRPGRYLLDDAGLTELRSFVDAGTLLAFDLDGTLAPIVADPSAIVIPGHIQESMARLCAMATVVILTGRGREDALSF
LGFEPCMVVGNHGAEGLPGWEEREREFRELCAGWKQQLHLSLAGTSSGIFLEDKRLSLSLHYRNAAGRGEAFATIIEAIG
ALVPAPERISGKFVENVVPRGAPRKGRALLDTLRYLGTGRALYVGDDATDEDVFRLRNERIFGVRVGASSSTAADYYLKD
QQEIDRVLREIIDILSHPSCDAA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=213, Percent_Identity=29.1079812206573, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129309, Length=256, Percent_Identity=27.34375, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28654; Mature: 28654

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNRPGRYLLDDAGLTELRSFVDAGTLLAFDLDGTLAPIVADPSAIVIPGHIQESMARL
CCCCCCCCEEECCCCHHHHHHHHCCCCEEEEECCCCEEEEEECCCEEEECCHHHHHHHHH
CAMATVVILTGRGREDALSFLGFEPCMVVGNHGAEGLPGWEEREREFRELCAGWKQQLHL
HHHHHHHEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEE
SLAGTSSGIFLEDKRLSLSLHYRNAAGRGEAFATIIEAIGALVPAPERISGKFVENVVPR
EEECCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCHHHHHHHCCC
GAPRKGRALLDTLRYLGTGRALYVGDDATDEDVFRLRNERIFGVRVGASSSTAADYYLKD
CCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCEEEEEEECCCCCCCHHHHCCC
QQEIDRVLREIIDILSHPSCDAA
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIQNRPGRYLLDDAGLTELRSFVDAGTLLAFDLDGTLAPIVADPSAIVIPGHIQESMARL
CCCCCCCCEEECCCCHHHHHHHHCCCCEEEEECCCCEEEEEECCCEEEECCHHHHHHHHH
CAMATVVILTGRGREDALSFLGFEPCMVVGNHGAEGLPGWEEREREFRELCAGWKQQLHL
HHHHHHHEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEE
SLAGTSSGIFLEDKRLSLSLHYRNAAGRGEAFATIIEAIGALVPAPERISGKFVENVVPR
EEECCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCHHHHHHHCCC
GAPRKGRALLDTLRYLGTGRALYVGDDATDEDVFRLRNERIFGVRVGASSSTAADYYLKD
CCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCEEEEEEECCCCCCCHHHHCCC
QQEIDRVLREIIDILSHPSCDAA
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]