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The map label for this gene is macA [H]

Identifier: 118581634

GI number: 118581634

Start: 3552375

End: 3553592

Strand: Direct

Name: macA [H]

Synonym: Ppro_3233

Alternate gene names: 118581634

Gene position: 3552375-3553592 (Clockwise)

Preceding gene: 118581633

Following gene: 118581635

Centisome position: 88.63

GC content: 64.86

Gene sequence:

>1218_bases
ATGAGAAAAACCGCGCTGATAGCCCTAGCGCTCCTGCTGGTAGCGGGAATCGCCATTTCCACCTACCTGAGACGGGCGCC
GGAAATCACCTACAGGACCGCCAGAATCCAACGGGGCACCATCGTCTCCGGCGTCTCCGCCACCGGAAACGTCAGCGCCG
TGACCACGGTCCAGGTGGGAACCCAGGTTTCCGGCACGATCCACAAGCTGTACGTGGACTACAACTCCCCGGTCAAAAAG
GGGCAGCCCATCGCGGAGATCGACCCCTCCCTGTTCAGGGCGGCGGTGGAGCAGTCCCGGGGCAACAATCTGGTTGCCAG
GGCCAACCTGCAGAAGGCCAGGGTAGTGCTGGTGGACGCCGAGCGTACCATGAAACGCAACAGCACGCTCCTGAAGGAGG
GGGTCATCTCCCAGGGTGATTTCGACACGGCGGAAACCGCCTACCAGTCAGCCAGGGCGGCGGTGGAGGCGGCCCAGGGG
ACGGTGGCCCAGACAGAGGGGGCGCTCATGCAGGCCCGCACCAACCTGAACTACTCCATCATCCGCTCGCCGGTGGACGG
CATCGTCATCTCCCGGGCAGTGGACGTGGGCCAGACCGTGGCGGCCTCCTTCCAGACCCCCACCCTCTTCACCATCGCTC
AGGACCTGACCAAGATGCAGATCGAGGTCAGCGTTGACGAGGCGGACATAAGCAGCATTAGGCAGAGCCAGACTGTGACC
TTCAGCGTCGACTCCTACCCGGAGCAATCCTTCAGGGGGAGGGTGACCCAGATCCGCAACGCGCCGATCATCACCCAGAA
CGTGGTCACCTATGTGGTTCTGGTAACCGTGGACAACAGCGACCTGAAGCTGAAACCGGGCATGACCGCCAACGTGACCA
TCGAGGTGGCCCGCAAGGCTTCCGTGCTCAGGCTTCCCCTGGCCGCCCTGCGCTTCAGGCCCAGGTCCGCGACGGACGCG
GGCACGACTCCGCGGCCCAGGAACAGCACCGCCACCCGCCCTCCCCAGCAGGCCACAGGCGAGCAGCGGGTCCACATCCT
CAGGGAGGGCAAGCCGGTGGCAATCGGCATCAGGACCGGCATCGCCGACGACAGCGCCATCGAGCTGACCGGAGGAGCGC
TCAAAGAGGGGGATCTGGTCGTCATCGAACAGATCGGCGGCACCGGAAAGAAGAAGGCCAACGCAGCGGGCGGTTCCCCC
ATGGGTCCGCGCTTCTAG

Upstream 100 bases:

>100_bases
GTTCCCGGAAAAGCCCCGGTCCTTGGACATGGGGCTTTTTTTTTGAAACTTTTCTGTTATCATCCCCGTCTGCACTGGAG
AGCCACGGGAGAGACATTCC

Downstream 100 bases:

>100_bases
CCATGGCAGAGGTCATCTCGCTCAGCGACATACGCCGCGTCTTCATCATGGGGGAGCAGCAGTTCGAGGCGCTCAGGGGG
ATCTCCTTCTCCATCGCCGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MRKTALIALALLLVAGIAISTYLRRAPEITYRTARIQRGTIVSGVSATGNVSAVTTVQVGTQVSGTIHKLYVDYNSPVKK
GQPIAEIDPSLFRAAVEQSRGNNLVARANLQKARVVLVDAERTMKRNSTLLKEGVISQGDFDTAETAYQSARAAVEAAQG
TVAQTEGALMQARTNLNYSIIRSPVDGIVISRAVDVGQTVAASFQTPTLFTIAQDLTKMQIEVSVDEADISSIRQSQTVT
FSVDSYPEQSFRGRVTQIRNAPIITQNVVTYVVLVTVDNSDLKLKPGMTANVTIEVARKASVLRLPLAALRFRPRSATDA
GTTPRPRNSTATRPPQQATGEQRVHILREGKPVAIGIRTGIADDSAIELTGGALKEGDLVVIEQIGGTGKKKANAAGGSP
MGPRF

Sequences:

>Translated_405_residues
MRKTALIALALLLVAGIAISTYLRRAPEITYRTARIQRGTIVSGVSATGNVSAVTTVQVGTQVSGTIHKLYVDYNSPVKK
GQPIAEIDPSLFRAAVEQSRGNNLVARANLQKARVVLVDAERTMKRNSTLLKEGVISQGDFDTAETAYQSARAAVEAAQG
TVAQTEGALMQARTNLNYSIIRSPVDGIVISRAVDVGQTVAASFQTPTLFTIAQDLTKMQIEVSVDEADISSIRQSQTVT
FSVDSYPEQSFRGRVTQIRNAPIITQNVVTYVVLVTVDNSDLKLKPGMTANVTIEVARKASVLRLPLAALRFRPRSATDA
GTTPRPRNSTATRPPQQATGEQRVHILREGKPVAIGIRTGIADDSAIELTGGALKEGDLVVIEQIGGTGKKKANAAGGSP
MGPRF
>Mature_405_residues
MRKTALIALALLLVAGIAISTYLRRAPEITYRTARIQRGTIVSGVSATGNVSAVTTVQVGTQVSGTIHKLYVDYNSPVKK
GQPIAEIDPSLFRAAVEQSRGNNLVARANLQKARVVLVDAERTMKRNSTLLKEGVISQGDFDTAETAYQSARAAVEAAQG
TVAQTEGALMQARTNLNYSIIRSPVDGIVISRAVDVGQTVAASFQTPTLFTIAQDLTKMQIEVSVDEADISSIRQSQTVT
FSVDSYPEQSFRGRVTQIRNAPIITQNVVTYVVLVTVDNSDLKLKPGMTANVTIEVARKASVLRLPLAALRFRPRSATDA
GTTPRPRNSTATRPPQQATGEQRVHILREGKPVAIGIRTGIADDSAIELTGGALKEGDLVVIEQIGGTGKKKANAAGGSP
MGPRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=382, Percent_Identity=29.3193717277487, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI87082036, Length=274, Percent_Identity=27.007299270073, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1790519, Length=254, Percent_Identity=29.1338582677165, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789929, Length=258, Percent_Identity=26.3565891472868, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43203; Mature: 43203

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTALIALALLLVAGIAISTYLRRAPEITYRTARIQRGTIVSGVSATGNVSAVTTVQVG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEECCCCCCCEEEEEEEEEC
TQVSGTIHKLYVDYNSPVKKGQPIAEIDPSLFRAAVEQSRGNNLVARANLQKARVVLVDA
CCCCCEEEEEEEECCCCHHCCCCHHHCCHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEC
ERTMKRNSTLLKEGVISQGDFDTAETAYQSARAAVEAAQGTVAQTEGALMQARTNLNYSI
HHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCEEE
IRSPVDGIVISRAVDVGQTVAASFQTPTLFTIAQDLTKMQIEVSVDEADISSIRQSQTVT
ECCCCCCEEEHHHHHCCHHHHHCCCCCCHHHHHHHHEEEEEEEEECHHHHHHHHCCCEEE
FSVDSYPEQSFRGRVTQIRNAPIITQNVVTYVVLVTVDNSDLKLKPGMTANVTIEVARKA
EEECCCCCHHHCCHHHHHCCCCCEEECCEEEEEEEEECCCCEEECCCCEEEEEEEEECCC
SVLRLPLAALRFRPRSATDAGTTPRPRNSTATRPPQQATGEQRVHILREGKPVAIGIRTG
HHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCEEEEEEEEC
IADDSAIELTGGALKEGDLVVIEQIGGTGKKKANAAGGSPMGPRF
CCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKTALIALALLLVAGIAISTYLRRAPEITYRTARIQRGTIVSGVSATGNVSAVTTVQVG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEECCCCCCCEEEEEEEEEC
TQVSGTIHKLYVDYNSPVKKGQPIAEIDPSLFRAAVEQSRGNNLVARANLQKARVVLVDA
CCCCCEEEEEEEECCCCHHCCCCHHHCCHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEC
ERTMKRNSTLLKEGVISQGDFDTAETAYQSARAAVEAAQGTVAQTEGALMQARTNLNYSI
HHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCEEE
IRSPVDGIVISRAVDVGQTVAASFQTPTLFTIAQDLTKMQIEVSVDEADISSIRQSQTVT
ECCCCCCEEEHHHHHCCHHHHHCCCCCCHHHHHHHHEEEEEEEEECHHHHHHHHCCCEEE
FSVDSYPEQSFRGRVTQIRNAPIITQNVVTYVVLVTVDNSDLKLKPGMTANVTIEVARKA
EEECCCCCHHHCCHHHHHCCCCCEEECCEEEEEEEEECCCCEEECCCCEEEEEEEEECCC
SVLRLPLAALRFRPRSATDAGTTPRPRNSTATRPPQQATGEQRVHILREGKPVAIGIRTG
HHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCEEEEEEEEC
IADDSAIELTGGALKEGDLVVIEQIGGTGKKKANAAGGSPMGPRF
CCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]