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The map label for this gene is pilT [H]

Identifier: 118581579

GI number: 118581579

Start: 3493059

End: 3494225

Strand: Reverse

Name: pilT [H]

Synonym: Ppro_3178

Alternate gene names: 118581579

Gene position: 3494225-3493059 (Counterclockwise)

Preceding gene: 118581580

Following gene: 118581578

Centisome position: 87.18

GC content: 61.18

Gene sequence:

>1167_bases
ATGAATCTGAACGAGATTCTCACCATTGCCGTCAAGGCCAAGGGTTCCGACATTCACATCAAGACCGGCCTGCCCCCCAT
CGTGCGCATCGACGGCAGGCTGCACCCCATCCCCAATGCCCCCCGGCTGGCACCCGATATCGTGGGAAACATGGCCCAGT
CCATGATGAACGACCGCCAGCGGCGCATCTTCGAGGAAAGTTCCGAGGTGGACCTGGCCTATGCCGTGCCCGGCCTGGGA
CGCTTCAGGGTCAGCGTGTACCGCCAGCGCGGCACCGTTGCCATGGTCTTTCGCGCCATCTCCTTCGCCATCCCCACCTT
GGATGGTCTCAACCTGCCGCCGGTGATCAAAAAGATGTGCATGGAGGAACGCGGCCTGATCTTGGTTACCGGCACCACCG
GTTCGGGCAAGTCCAGTACCCTGGCGGCCATGATCGATACCATCAACCAGAGCCGCACCTGCAACATCATCACCATCGAA
GACCCGGTGGAGTTCCTGCACCGAGACAACAAGAGCATCATCAGTCAGCGTGAGGTGGGTACGGATACCCCAACCTTTTC
CGGAGCGCTCAAGGGTGCACTGCGTCAGGACCCGGACGTGATCCTGGTGGGCGAGATGCGCGACTACGAGACCATCGAGA
CCGCCCTGACCGCGGCCGAGACCGGCCACCTGGTCATGTCCACCCTGCACACCCTGGATGCCGCCGAGACGGTCAACCGC
ATCATCAGTGTGTTCCCCCCCTACCACCAGCGCCAGGTTCGCATGCAACTGGCCGGGGTCATCAAGGGGGTTATCTCCCA
GCGCCTGGTGCCCCGGGCCGACGGCAAGGGACGCGTGCCGGCGGTGGAGATCCTGCTGGGAACCGCCCGCGTGCGGGAAT
GCATCGACGACAAGGACAAGACCAAGCAGCTGGGAGACGCCATCGCCCAGGGTTTCATCTCCTACGGCATGCAGACCTTC
GACCAGTCGCTGATGAAGCTGTTCAGCTCCAAGATGATCACCTACGAAGAGGCCTTGAGGCAGTGCTCCAACCCGGATGA
CTTTGCCCTCAAGATCTCCGGCATCTCGTCCACTTCCGATTCCTCCTGGGATGGTTTCGAGCACAGGGAAGATGACGCTT
CCGAGCCGGTCAAAGTTGACGACAAGATCGAGATCGAAAAATACTGA

Upstream 100 bases:

>100_bases
TTTCTGACCGAGCATCCCGAGGTGCTGGCGGATATCGAGGGAAAACTGATGGACCTGCTCAGGGGGCCGGCCGTTACCAA
CGAGTAGGGGGAGCTTTGCC

Downstream 100 bases:

>100_bases
CCCATCCTCTCCCGAACAGGGGAGGCAGTACGCCCTGAGACTCCTGGCCGGCCGCGACTACACGGTTGCCGCCTTGAAAC
AGAAGCTTCGTGCCAGGCAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNLNEILTIAVKAKGSDIHIKTGLPPIVRIDGRLHPIPNAPRLAPDIVGNMAQSMMNDRQRRIFEESSEVDLAYAVPGLG
RFRVSVYRQRGTVAMVFRAISFAIPTLDGLNLPPVIKKMCMEERGLILVTGTTGSGKSSTLAAMIDTINQSRTCNIITIE
DPVEFLHRDNKSIISQREVGTDTPTFSGALKGALRQDPDVILVGEMRDYETIETALTAAETGHLVMSTLHTLDAAETVNR
IISVFPPYHQRQVRMQLAGVIKGVISQRLVPRADGKGRVPAVEILLGTARVRECIDDKDKTKQLGDAIAQGFISYGMQTF
DQSLMKLFSSKMITYEEALRQCSNPDDFALKISGISSTSDSSWDGFEHREDDASEPVKVDDKIEIEKY

Sequences:

>Translated_388_residues
MNLNEILTIAVKAKGSDIHIKTGLPPIVRIDGRLHPIPNAPRLAPDIVGNMAQSMMNDRQRRIFEESSEVDLAYAVPGLG
RFRVSVYRQRGTVAMVFRAISFAIPTLDGLNLPPVIKKMCMEERGLILVTGTTGSGKSSTLAAMIDTINQSRTCNIITIE
DPVEFLHRDNKSIISQREVGTDTPTFSGALKGALRQDPDVILVGEMRDYETIETALTAAETGHLVMSTLHTLDAAETVNR
IISVFPPYHQRQVRMQLAGVIKGVISQRLVPRADGKGRVPAVEILLGTARVRECIDDKDKTKQLGDAIAQGFISYGMQTF
DQSLMKLFSSKMITYEEALRQCSNPDDFALKISGISSTSDSSWDGFEHREDDASEPVKVDDKIEIEKY
>Mature_388_residues
MNLNEILTIAVKAKGSDIHIKTGLPPIVRIDGRLHPIPNAPRLAPDIVGNMAQSMMNDRQRRIFEESSEVDLAYAVPGLG
RFRVSVYRQRGTVAMVFRAISFAIPTLDGLNLPPVIKKMCMEERGLILVTGTTGSGKSSTLAAMIDTINQSRTCNIITIE
DPVEFLHRDNKSIISQREVGTDTPTFSGALKGALRQDPDVILVGEMRDYETIETALTAAETGHLVMSTLHTLDAAETVNR
IISVFPPYHQRQVRMQLAGVIKGVISQRLVPRADGKGRVPAVEILLGTARVRECIDDKDKTKQLGDAIAQGFISYGMQTF
DQSLMKLFSSKMITYEEALRQCSNPDDFALKISGISSTSDSSWDGFEHREDDASEPVKVDDKIEIEKY

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=323, Percent_Identity=43.6532507739938, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=35.9430604982206, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789723, Length=291, Percent_Identity=34.020618556701, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42819; Mature: 42819

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNEILTIAVKAKGSDIHIKTGLPPIVRIDGRLHPIPNAPRLAPDIVGNMAQSMMNDRQ
CCCCCEEEEEEEECCCEEEEECCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHH
RRIFEESSEVDLAYAVPGLGRFRVSVYRQRGTVAMVFRAISFAIPTLDGLNLPPVIKKMC
HHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
MEERGLILVTGTTGSGKSSTLAAMIDTINQSRTCNIITIEDPVEFLHRDNKSIISQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHCC
TDTPTFSGALKGALRQDPDVILVGEMRDYETIETALTAAETGHLVMSTLHTLDAAETVNR
CCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISVFPPYHQRQVRMQLAGVIKGVISQRLVPRADGKGRVPAVEILLGTARVRECIDDKDK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
TKQLGDAIAQGFISYGMQTFDQSLMKLFSSKMITYEEALRQCSNPDDFALKISGISSTSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
SSWDGFEHREDDASEPVKVDDKIEIEKY
CCCCCCCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MNLNEILTIAVKAKGSDIHIKTGLPPIVRIDGRLHPIPNAPRLAPDIVGNMAQSMMNDRQ
CCCCCEEEEEEEECCCEEEEECCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHH
RRIFEESSEVDLAYAVPGLGRFRVSVYRQRGTVAMVFRAISFAIPTLDGLNLPPVIKKMC
HHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
MEERGLILVTGTTGSGKSSTLAAMIDTINQSRTCNIITIEDPVEFLHRDNKSIISQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHCC
TDTPTFSGALKGALRQDPDVILVGEMRDYETIETALTAAETGHLVMSTLHTLDAAETVNR
CCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISVFPPYHQRQVRMQLAGVIKGVISQRLVPRADGKGRVPAVEILLGTARVRECIDDKDK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
TKQLGDAIAQGFISYGMQTFDQSLMKLFSSKMITYEEALRQCSNPDDFALKISGISSTSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
SSWDGFEHREDDASEPVKVDDKIEIEKY
CCCCCCCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]