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The map label for this gene is ntrB [H]

Identifier: 118581540

GI number: 118581540

Start: 3440478

End: 3442652

Strand: Direct

Name: ntrB [H]

Synonym: Ppro_3139

Alternate gene names: 118581540

Gene position: 3440478-3442652 (Clockwise)

Preceding gene: 118581535

Following gene: 118581541

Centisome position: 85.84

GC content: 61.56

Gene sequence:

>2175_bases
TTGTTGCAGATACCAGCAGCAATTCTTGACTCAATCGAATCACTGCATCTGCCACCTATCCCGCAGATTCTGTTGAGATT
TCTGCATCTTACCGAAGACGACCGCGCAACGCTCAGTGACCTTGCCGCGCTGGTGGGGCAGGACCCCTCCCTGAGCGCAC
GCATCTTCACCGTTGCCAACTCTCCGGCTCTCTGCCACGGTAGAGAGTCAAAGAGCCTCACCCAGTGCCTGGTAACCCTT
GGCACACGCCTGTCACGAACCCTGGCCGCCTGCCTGGCTGTCCAGAGAGTCTTTGCCCCCACCATTGACAAGCAGCAGTA
CAGTCTCACCGGCTTCTGGAGCCATTCCCTGCGGGTTGCCGAAACGTCCCGCGCCATTGCGGACGAAATCAACTACATCG
ATCCGGAAGAGGCCTACATCTCCGGCCTGCTGCACGATGTGGGGCAACTACTCCTCCTGGGGGGAATGGGAAAGCACTAC
GGCGCCCTGCTGGCCATGAGCAACGATGAGACGGAACTCCGCGATGCCGAACTGCGCGTTCTCCACACCGACCACGCCAT
GATCGGCGCCTGGCTGATCGACCAGTGGAAGCTCTCCTCTTTTATGGCCGACGCGGTCCTCTTTCACCACAGCCCCCCCG
ACGCGATCGTCACCGCCGACAGCCTGAGCCAGATCGTCTGGTCCGCCCACACCTTCTGCAACGCCCCTCTCTCTCCCGAA
TCCGGCCAGCCTCTCAAAACCGTTGAATTCGATACGATAAACGCCATACTCGGTATCAGTGACCAAAGGCTATCGGCCCT
GGAGCAGCGCTGTTCGGAACGGGTCGCCCTTATCGCCGAAACGCTCGAAATCCGCGAGGCCGGCATCCAGAAGACACTAC
CTGCGGCAACCACCCCGCTGGAAGCAACGCAACAAAAACAGCGGACCGCCGACCCTTCCCTCAGCCTGCTGGAGGAGACC
GTACGGGACATGGCCCTGATGCACCCCCTGCAACAGGATCTCGTCTCGGTGGACAACGAATCCGACCTGCTCCTGACCGT
GCGCGAATCGGCCCGCATACTCTTCGGCCTGGGGCGGACGGCTTTTCTGCTGGCACAGCCGGACGGAAAGACTCTCTCCG
GCGCCCCCAGCCATGGCCAGCTGGCCATGCTGCGGCAGCTGGAAATCCCGCTGGATTCCGGCCAGAGCCTGGCGGCAACC
AGCTCACGGGACAGGCGCTCCTACTGCACCTTTTCCAGCGATCTCCCCGCCCCCATCTCGCTGGTCGATGTCCAGATCAG
CCGGATACTGGGAAGCGAGGGACTGCTGTATGTCCCCCTGGCAAGCCGCGGCAGAACAATCGGCGTTATGGTCTACGGCG
TCAGCGCCACGCAATCGGGCCGGGTTCTCGAACAGCTCCCCTGGATAGACAGCTTCGCCCAGGTCGCCGCCGATAGCATG
GGCGTCTGGCGCGAGATGCGCACACAAGAACAGCGGATCGAAACAACACTGACGGCACGCTTTGAACGCCAGACCCGCAG
GGCGACCCATGAGGCCGGCAACCCACTGGGAATCATCAAAAACTACCTGAGCATCATCAGCAACAAACTCAGCGACCGCC
CAGAAATACTGCAGGAAATGGGCATCCTGCGCGAGGAAATCGACCGCGTTACCGCTATCATGCAGCGCATGAACAGCCTC
CCGGACGAGCTCCCCCCGGCGGATTCCCTGGACATCAACGCCCTGATCGAGAGCATGCTGGCGCTGTATGGCGACACTCT
TTTCACCGAACGGGGAATCGCCCTGGAAAAGTCACTGGACTCCAGGATAAGGCCGACCAGCATTGACCGGGACAGCCTCA
AGCAGATCCTGACCAACCTCTGGAACAATGCTGCCGATGCCATGGGGACCGGCGACTGCTTCTTCATCGCCACCCATGCC
GATGTCAACCAGAATGGCCGCCCCTACGTGGAAATCAGCATGACCGATACCGGCCCCGGCCTGCCGGCGGATGTCATGGA
GCGCCTCTTCCAGCCGCTGGAGCCCACCAGGCGCCCCGACCATGGCGGAGTCGGCCTCTCCATCGTGGCAGGACTGGTTG
AACGCATGCATGGCCGCATCACCTGCCAGAGCAGGAGGGGACAGGGAACCACATTTTCTATCCTGCTGCCGCACTCCGGG
AGGGACGAGTCGTGA

Upstream 100 bases:

>100_bases
TATTTTTACCGCCAGATATGTTCATATCCCACAATTATTCGCCATATTTGACAGTTAGGCCACATATCCAGGCTACAGGA
ACCGCAACGGGGGAGACGGT

Downstream 100 bases:

>100_bases
ACAGCCTGAACACGACAACCAGGGCTGCCCTTGAGCGTCACGACAGGCCGCACAGCATCCTGATTGTGGATGACGAGCCG
CGCATGCGCACAAGCCTGCG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MLQIPAAILDSIESLHLPPIPQILLRFLHLTEDDRATLSDLAALVGQDPSLSARIFTVANSPALCHGRESKSLTQCLVTL
GTRLSRTLAACLAVQRVFAPTIDKQQYSLTGFWSHSLRVAETSRAIADEINYIDPEEAYISGLLHDVGQLLLLGGMGKHY
GALLAMSNDETELRDAELRVLHTDHAMIGAWLIDQWKLSSFMADAVLFHHSPPDAIVTADSLSQIVWSAHTFCNAPLSPE
SGQPLKTVEFDTINAILGISDQRLSALEQRCSERVALIAETLEIREAGIQKTLPAATTPLEATQQKQRTADPSLSLLEET
VRDMALMHPLQQDLVSVDNESDLLLTVRESARILFGLGRTAFLLAQPDGKTLSGAPSHGQLAMLRQLEIPLDSGQSLAAT
SSRDRRSYCTFSSDLPAPISLVDVQISRILGSEGLLYVPLASRGRTIGVMVYGVSATQSGRVLEQLPWIDSFAQVAADSM
GVWREMRTQEQRIETTLTARFERQTRRATHEAGNPLGIIKNYLSIISNKLSDRPEILQEMGILREEIDRVTAIMQRMNSL
PDELPPADSLDINALIESMLALYGDTLFTERGIALEKSLDSRIRPTSIDRDSLKQILTNLWNNAADAMGTGDCFFIATHA
DVNQNGRPYVEISMTDTGPGLPADVMERLFQPLEPTRRPDHGGVGLSIVAGLVERMHGRITCQSRRGQGTTFSILLPHSG
RDES

Sequences:

>Translated_724_residues
MLQIPAAILDSIESLHLPPIPQILLRFLHLTEDDRATLSDLAALVGQDPSLSARIFTVANSPALCHGRESKSLTQCLVTL
GTRLSRTLAACLAVQRVFAPTIDKQQYSLTGFWSHSLRVAETSRAIADEINYIDPEEAYISGLLHDVGQLLLLGGMGKHY
GALLAMSNDETELRDAELRVLHTDHAMIGAWLIDQWKLSSFMADAVLFHHSPPDAIVTADSLSQIVWSAHTFCNAPLSPE
SGQPLKTVEFDTINAILGISDQRLSALEQRCSERVALIAETLEIREAGIQKTLPAATTPLEATQQKQRTADPSLSLLEET
VRDMALMHPLQQDLVSVDNESDLLLTVRESARILFGLGRTAFLLAQPDGKTLSGAPSHGQLAMLRQLEIPLDSGQSLAAT
SSRDRRSYCTFSSDLPAPISLVDVQISRILGSEGLLYVPLASRGRTIGVMVYGVSATQSGRVLEQLPWIDSFAQVAADSM
GVWREMRTQEQRIETTLTARFERQTRRATHEAGNPLGIIKNYLSIISNKLSDRPEILQEMGILREEIDRVTAIMQRMNSL
PDELPPADSLDINALIESMLALYGDTLFTERGIALEKSLDSRIRPTSIDRDSLKQILTNLWNNAADAMGTGDCFFIATHA
DVNQNGRPYVEISMTDTGPGLPADVMERLFQPLEPTRRPDHGGVGLSIVAGLVERMHGRITCQSRRGQGTTFSILLPHSG
RDES
>Mature_724_residues
MLQIPAAILDSIESLHLPPIPQILLRFLHLTEDDRATLSDLAALVGQDPSLSARIFTVANSPALCHGRESKSLTQCLVTL
GTRLSRTLAACLAVQRVFAPTIDKQQYSLTGFWSHSLRVAETSRAIADEINYIDPEEAYISGLLHDVGQLLLLGGMGKHY
GALLAMSNDETELRDAELRVLHTDHAMIGAWLIDQWKLSSFMADAVLFHHSPPDAIVTADSLSQIVWSAHTFCNAPLSPE
SGQPLKTVEFDTINAILGISDQRLSALEQRCSERVALIAETLEIREAGIQKTLPAATTPLEATQQKQRTADPSLSLLEET
VRDMALMHPLQQDLVSVDNESDLLLTVRESARILFGLGRTAFLLAQPDGKTLSGAPSHGQLAMLRQLEIPLDSGQSLAAT
SSRDRRSYCTFSSDLPAPISLVDVQISRILGSEGLLYVPLASRGRTIGVMVYGVSATQSGRVLEQLPWIDSFAQVAADSM
GVWREMRTQEQRIETTLTARFERQTRRATHEAGNPLGIIKNYLSIISNKLSDRPEILQEMGILREEIDRVTAIMQRMNSL
PDELPPADSLDINALIESMLALYGDTLFTERGIALEKSLDSRIRPTSIDRDSLKQILTNLWNNAADAMGTGDCFFIATHA
DVNQNGRPYVEISMTDTGPGLPADVMERLFQPLEPTRRPDHGGVGLSIVAGLVERMHGRITCQSRRGQGTTFSILLPHSG
RDES

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=210, Percent_Identity=30.952380952381, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=28.4552845528455, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790300, Length=223, Percent_Identity=27.8026905829596, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 79450; Mature: 79450

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQIPAAILDSIESLHLPPIPQILLRFLHLTEDDRATLSDLAALVGQDPSLSARIFTVAN
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEC
SPALCHGRESKSLTQCLVTLGTRLSRTLAACLAVQRVFAPTIDKQQYSLTGFWSHSLRVA
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEHH
ETSRAIADEINYIDPEEAYISGLLHDVGQLLLLGGMGKHYGALLAMSNDETELRDAELRV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHCCCEEEE
LHTDHAMIGAWLIDQWKLSSFMADAVLFHHSPPDAIVTADSLSQIVWSAHTFCNAPLSPE
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCC
SGQPLKTVEFDTINAILGISDQRLSALEQRCSERVALIAETLEIREAGIQKTLPAATTPL
CCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EATQQKQRTADPSLSLLEETVRDMALMHPLQQDLVSVDNESDLLLTVRESARILFGLGRT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCE
AFLLAQPDGKTLSGAPSHGQLAMLRQLEIPLDSGQSLAATSSRDRRSYCTFSSDLPAPIS
EEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHH
LVDVQISRILGSEGLLYVPLASRGRTIGVMVYGVSATQSGRVLEQLPWIDSFAQVAADSM
HHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHH
GVWREMRTQEQRIETTLTARFERQTRRATHEAGNPLGIIKNYLSIISNKLSDRPEILQEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
GILREEIDRVTAIMQRMNSLPDELPPADSLDINALIESMLALYGDTLFTERGIALEKSLD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHH
SRIRPTSIDRDSLKQILTNLWNNAADAMGTGDCFFIATHADVNQNGRPYVEISMTDTGPG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCC
LPADVMERLFQPLEPTRRPDHGGVGLSIVAGLVERMHGRITCQSRRGQGTTFSILLPHSG
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCC
RDES
CCCC
>Mature Secondary Structure
MLQIPAAILDSIESLHLPPIPQILLRFLHLTEDDRATLSDLAALVGQDPSLSARIFTVAN
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEC
SPALCHGRESKSLTQCLVTLGTRLSRTLAACLAVQRVFAPTIDKQQYSLTGFWSHSLRVA
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEHH
ETSRAIADEINYIDPEEAYISGLLHDVGQLLLLGGMGKHYGALLAMSNDETELRDAELRV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHCCCEEEE
LHTDHAMIGAWLIDQWKLSSFMADAVLFHHSPPDAIVTADSLSQIVWSAHTFCNAPLSPE
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCC
SGQPLKTVEFDTINAILGISDQRLSALEQRCSERVALIAETLEIREAGIQKTLPAATTPL
CCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EATQQKQRTADPSLSLLEETVRDMALMHPLQQDLVSVDNESDLLLTVRESARILFGLGRT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCE
AFLLAQPDGKTLSGAPSHGQLAMLRQLEIPLDSGQSLAATSSRDRRSYCTFSSDLPAPIS
EEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHH
LVDVQISRILGSEGLLYVPLASRGRTIGVMVYGVSATQSGRVLEQLPWIDSFAQVAADSM
HHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHH
GVWREMRTQEQRIETTLTARFERQTRRATHEAGNPLGIIKNYLSIISNKLSDRPEILQEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
GILREEIDRVTAIMQRMNSLPDELPPADSLDINALIESMLALYGDTLFTERGIALEKSLD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHH
SRIRPTSIDRDSLKQILTNLWNNAADAMGTGDCFFIATHADVNQNGRPYVEISMTDTGPG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCC
LPADVMERLFQPLEPTRRPDHGGVGLSIVAGLVERMHGRITCQSRRGQGTTFSILLPHSG
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCC
RDES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]