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The map label for this gene is dnaB [H]

Identifier: 118581527

GI number: 118581527

Start: 3427649

End: 3428908

Strand: Direct

Name: dnaB [H]

Synonym: Ppro_3126

Alternate gene names: 118581527

Gene position: 3427649-3428908 (Clockwise)

Preceding gene: 118581526

Following gene: 118581528

Centisome position: 85.52

GC content: 55.95

Gene sequence:

>1260_bases
ATGAACCAATTCACTGACCCTGTTGCCGAGCAAGAGATTGTCGGAGCCGTCCTGACGGACCCTGATTCCTTTACAAGCAT
CCGCCCCATTGTCACCCCGGAGATGTTCTCAAATCTGAAGCTGGAAACGGTCTACAGGACATTTTCTGAACAGTACGAGA
AAAACGGACTCACTGACCTTGCAACCCTGGCGGAAATATCTGGAGTACCAGGACACGTCCTTGTTGGCTGTATGGCGGAA
AGCGCTATTCCATCGCTGGTACACATCAAGGCCCGCAAAATTGCCGACCAGTTCCACAAGCGCCATATTCAACGCCAGTT
GGCGATTACCATCGCCCAGCTGGGAACCCTCACCCCGGCAGAGATCGCAGCAGCACTCTCCAACTTGGCAGCAGACGTGA
CGATCAGGAACCATGAAAAATCCGTATTCTCTCCCGGCGAGTTGTGCAAACGAGTTTCCGCTCTCCAGAAGACAAGGGAG
CAGGAACGAAAGGAAGGCAGGCACCAAGGGGCGGACACGGGCTATCCGGTTGTACAGCAGCTGCTCAAGGGGCTTGCGCC
GAAACGAATGACGGTCATCGCCGCCGCTACCGGCTTCGGCAAGAGCACCCTGGCGCTCAATCTGCTTTACAACACGACCA
CAAGCGGACACCGGGCGCTGTTCATCACCAACGAAAACGACGTGGACACCAACCTTGACCGGCTCGCCGCGATTCACTCC
GGCATCCGGTTGAAGGAGCTTGAGCAAGGAGGACAACACCGGGCTGCCATCAGTTTTGGTGAGCAGTTCCACAACTCCGG
CCTGTTTATGACCGACAATTCCCCCCGGACCGTTGAGGAGATCGTGGGGATCATCACCCGACATGCGATTCAACACAAAA
TCAAAGTGGCCTTCGTTGATTACATCGGCGAGATCACCCCGAGCGGGGAGAGCTCCCGCGATACGGAAGAGGCAAGGCTT
GCCCGTTATGCACAGCGGCTTGTTGATTGCGCGAAATCGCTAGAGCTCCATGTGGTCGTTATGGCGCAGTTAAACCGCCA
AGGCAATACCAAGGGTAGGCCCACACGCGCCGAACTCGCTGGATGCTTCAAGATTGCCCAGAAAGCACACTCCTTACTGC
TGTTTTGGCAGGACGAGAAGAAGCGCGACGTTTTGACCGTGGATAAGAATCGCCAGGGGCCAAGCGGGATCGACATCCAC
ATGGACTTCGACCGGACAACGCAGCGCATCCGAGAGTTATGCGTTTTGACCGCTGACTAG

Upstream 100 bases:

>100_bases
GCTTCCTGAACAACTGGCTGATGAAGGCCCAGGACAGGGCGCCACGAGTGACGGGCGTGACCGATGACCGCAAAACCCGT
GCTGAGATCGAGGAACTGCT

Downstream 100 bases:

>100_bases
GGGAGTGGGGCTTATGAAACCGAGACCATCAAAAAAGCAAAGCCCCGGGTGCCCCTCCTGGATCTTGGCCGGCGTTCGGG
AGAAGTACGAAGAGGCCACA

Product: DnaB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNQFTDPVAEQEIVGAVLTDPDSFTSIRPIVTPEMFSNLKLETVYRTFSEQYEKNGLTDLATLAEISGVPGHVLVGCMAE
SAIPSLVHIKARKIADQFHKRHIQRQLAITIAQLGTLTPAEIAAALSNLAADVTIRNHEKSVFSPGELCKRVSALQKTRE
QERKEGRHQGADTGYPVVQQLLKGLAPKRMTVIAAATGFGKSTLALNLLYNTTTSGHRALFITNENDVDTNLDRLAAIHS
GIRLKELEQGGQHRAAISFGEQFHNSGLFMTDNSPRTVEEIVGIITRHAIQHKIKVAFVDYIGEITPSGESSRDTEEARL
ARYAQRLVDCAKSLELHVVVMAQLNRQGNTKGRPTRAELAGCFKIAQKAHSLLLFWQDEKKRDVLTVDKNRQGPSGIDIH
MDFDRTTQRIRELCVLTAD

Sequences:

>Translated_419_residues
MNQFTDPVAEQEIVGAVLTDPDSFTSIRPIVTPEMFSNLKLETVYRTFSEQYEKNGLTDLATLAEISGVPGHVLVGCMAE
SAIPSLVHIKARKIADQFHKRHIQRQLAITIAQLGTLTPAEIAAALSNLAADVTIRNHEKSVFSPGELCKRVSALQKTRE
QERKEGRHQGADTGYPVVQQLLKGLAPKRMTVIAAATGFGKSTLALNLLYNTTTSGHRALFITNENDVDTNLDRLAAIHS
GIRLKELEQGGQHRAAISFGEQFHNSGLFMTDNSPRTVEEIVGIITRHAIQHKIKVAFVDYIGEITPSGESSRDTEEARL
ARYAQRLVDCAKSLELHVVVMAQLNRQGNTKGRPTRAELAGCFKIAQKAHSLLLFWQDEKKRDVLTVDKNRQGPSGIDIH
MDFDRTTQRIRELCVLTAD
>Mature_419_residues
MNQFTDPVAEQEIVGAVLTDPDSFTSIRPIVTPEMFSNLKLETVYRTFSEQYEKNGLTDLATLAEISGVPGHVLVGCMAE
SAIPSLVHIKARKIADQFHKRHIQRQLAITIAQLGTLTPAEIAAALSNLAADVTIRNHEKSVFSPGELCKRVSALQKTRE
QERKEGRHQGADTGYPVVQQLLKGLAPKRMTVIAAATGFGKSTLALNLLYNTTTSGHRALFITNENDVDTNLDRLAAIHS
GIRLKELEQGGQHRAAISFGEQFHNSGLFMTDNSPRTVEEIVGIITRHAIQHKIKVAFVDYIGEITPSGESSRDTEEARL
ARYAQRLVDCAKSLELHVVVMAQLNRQGNTKGRPTRAELAGCFKIAQKAHSLLLFWQDEKKRDVLTVDKNRQGPSGIDIH
MDFDRTTQRIRELCVLTAD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=421, Percent_Identity=23.7529691211401, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 46377; Mature: 46377

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFTDPVAEQEIVGAVLTDPDSFTSIRPIVTPEMFSNLKLETVYRTFSEQYEKNGLTDL
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHH
ATLAEISGVPGHVLVGCMAESAIPSLVHIKARKIADQFHKRHIQRQLAITIAQLGTLTPA
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EIAAALSNLAADVTIRNHEKSVFSPGELCKRVSALQKTREQERKEGRHQGADTGYPVVQQ
HHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LLKGLAPKRMTVIAAATGFGKSTLALNLLYNTTTSGHRALFITNENDVDTNLDRLAAIHS
HHHCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHC
GIRLKELEQGGQHRAAISFGEQFHNSGLFMTDNSPRTVEEIVGIITRHAIQHKIKVAFVD
CCCHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YIGEITPSGESSRDTEEARLARYAQRLVDCAKSLELHVVVMAQLNRQGNTKGRPTRAELA
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCHHHHH
GCFKIAQKAHSLLLFWQDEKKRDVLTVDKNRQGPSGIDIHMDFDRTTQRIRELCVLTAD
HHHHHHHHHHHEEEEEECCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQFTDPVAEQEIVGAVLTDPDSFTSIRPIVTPEMFSNLKLETVYRTFSEQYEKNGLTDL
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHH
ATLAEISGVPGHVLVGCMAESAIPSLVHIKARKIADQFHKRHIQRQLAITIAQLGTLTPA
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EIAAALSNLAADVTIRNHEKSVFSPGELCKRVSALQKTREQERKEGRHQGADTGYPVVQQ
HHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LLKGLAPKRMTVIAAATGFGKSTLALNLLYNTTTSGHRALFITNENDVDTNLDRLAAIHS
HHHCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHC
GIRLKELEQGGQHRAAISFGEQFHNSGLFMTDNSPRTVEEIVGIITRHAIQHKIKVAFVD
CCCHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YIGEITPSGESSRDTEEARLARYAQRLVDCAKSLELHVVVMAQLNRQGNTKGRPTRAELA
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCHHHHH
GCFKIAQKAHSLLLFWQDEKKRDVLTVDKNRQGPSGIDIHMDFDRTTQRIRELCVLTAD
HHHHHHHHHHHEEEEEECCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]