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The map label for this gene is pilJ [H]

Identifier: 118581508

GI number: 118581508

Start: 3407012

End: 3408640

Strand: Reverse

Name: pilJ [H]

Synonym: Ppro_3107

Alternate gene names: 118581508

Gene position: 3408640-3407012 (Counterclockwise)

Preceding gene: 118581509

Following gene: 118581507

Centisome position: 85.05

GC content: 61.94

Gene sequence:

>1629_bases
ATGTCAACATTACTGAATATGAAGATCGGAGCCCGGCTCGGGTTTGGTTTCGGTTTCGTGCTGCTGCTGCTGGTCGCCAT
GGGAGGGATCAGCATCAGGAACATGGGCGCCATCAATCGCGGGGTGGAACAGATCGTGACGGTGGATTACGCCAAGATCA
ACCTGGCCAATGATATCAACGCGAGGGTTGTCACTCTCCTCGAAAATTTCGCGTTGATGCAGTTGAAGGACCAGACCGGA
AAGATTGAGGTGAAACGGGATATCGACACACTCCGTTCCGAATACCGTGGTCTGATGGAGAAGCTGGAGAAGCTGGAGAC
GACCGCCGAAGGGAAAGGGCTGATTGCCGCGGCCAAGGTTGCGCTGGCAAACGGCACAACCGCCAACAATCGGGTGATAG
AACTGAGCCTTGCCAACAGTGCGGCCGAAGCCCTTTCGGTGTACAACAACGAGGCGTATCCCCTGAACCGGAAGATCAAG
GCCGCCTTCAACGACATCGTCAAGTACCAGGAAGGGGTGATGGAGGTCAGTCATGGTGAGGCGACAAGGGTGTTCGGGAG
TACCCGCCTGATCAACGTCGTCCTTGTCGTCGTGGCGCTGCTGGTCGGCATCCTGACCAGTTATCTGATCACCCTCAGCA
TCACCCGCCCCCTGGCCGAGGGGATCAGGATCGCCGATGCCCTTGCCGACGGCAACCTGACCATCCAGGTCTCCTCCACG
GGTAGGGACGAGACCGGCCTGCTCATAGAGCACCTGGGGCACATGGTGCACAACCTGCGCGACATGATCTCCCGCACCGT
GGACATCTCGTCAGGTATCGCCGCCGCCTCCAACCAGCTGCATTCCACCGCCACCCAGATCGCCACCGGTGCGGAGGAAG
TGTCCTGCCAGGCCAATACCGTGGCCACCGCCAGCGAGGAGATGTCGGCAACCAGCAGCGACATCGCCCGGAACTGCTCC
ATGGCCGCCGATGCCTCCCGCCAGACCAGCGAATCGGCCAATTCCGGCGCCCAGGTGGTCAACGAGACCATCACCGGCAT
GAACGTGATCGCCGACCGCGTGCGTGAAACCTCCAAAACCATCGAGGCCCTGGGCACCCGCTCGGACCAGATCGGGGAGA
TCATCGGCACCATCGAGGATATCGCCGACCAGACCAACCTGCTGGCGCTGAACGCGGCCATCGAGGCGGCCCGTGCCGGG
GAACAGGGCCGCGGCTTCGCCGTGGTCGCCGATGAGGTGCGGGCACTGGCGGAGCGGACCACCAAGGCCACCCGGGAGAT
CGGGGAAATGATCAAGGCCATCCAGAGGGAAACCCAGGAAGCGGTCACTGCCATGGACGAGGGGGTGCGCGAAGTGGAAA
AGGGAGCGGAATCGTCGCAGAAATCGGGTCGGGCCCTGGAGGAAATCCTGGAGCGGATCAACGAAGTCAGCATGCAGGTC
AACCAGATCGCCACGGCGGCCGAAGAACAGACCGCGACCACCGGAGAGGTGACCAGCAACATCCAGCAGATCAACGAGGT
CGTCCACAGCACGGCCCAGGGAGCCGAGGAAACCGCCAAGGCTGCCGCCCAGTTGGCCGGTCAGGCCGAAGAGTTGCAGG
GTCTCGTCAGCCGCTTCCGGCTGTCCTAG

Upstream 100 bases:

>100_bases
CGCCGCCGTGGCGGAACTGCTGGATGACGCGCTCCGGCGCAGGTGATGGGAAAATGGTCATGGACGGTAAGGTGGCCGGA
CAAAAAAAGGAGGTCAGGCA

Downstream 100 bases:

>100_bases
GAGGAGCGTCGCCATGAGCAACCTGCCATCGGTTGTAGGGGAGAGGAGTGGTGCCTCCCGGGAGCTGATCCAGCTGGTCA
GCTTCGAACTGGGGGGTGAG

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSTLLNMKIGARLGFGFGFVLLLLVAMGGISIRNMGAINRGVEQIVTVDYAKINLANDINARVVTLLENFALMQLKDQTG
KIEVKRDIDTLRSEYRGLMEKLEKLETTAEGKGLIAAAKVALANGTTANNRVIELSLANSAAEALSVYNNEAYPLNRKIK
AAFNDIVKYQEGVMEVSHGEATRVFGSTRLINVVLVVVALLVGILTSYLITLSITRPLAEGIRIADALADGNLTIQVSST
GRDETGLLIEHLGHMVHNLRDMISRTVDISSGIAAASNQLHSTATQIATGAEEVSCQANTVATASEEMSATSSDIARNCS
MAADASRQTSESANSGAQVVNETITGMNVIADRVRETSKTIEALGTRSDQIGEIIGTIEDIADQTNLLALNAAIEAARAG
EQGRGFAVVADEVRALAERTTKATREIGEMIKAIQRETQEAVTAMDEGVREVEKGAESSQKSGRALEEILERINEVSMQV
NQIATAAEEQTATTGEVTSNIQQINEVVHSTAQGAEETAKAAAQLAGQAEELQGLVSRFRLS

Sequences:

>Translated_542_residues
MSTLLNMKIGARLGFGFGFVLLLLVAMGGISIRNMGAINRGVEQIVTVDYAKINLANDINARVVTLLENFALMQLKDQTG
KIEVKRDIDTLRSEYRGLMEKLEKLETTAEGKGLIAAAKVALANGTTANNRVIELSLANSAAEALSVYNNEAYPLNRKIK
AAFNDIVKYQEGVMEVSHGEATRVFGSTRLINVVLVVVALLVGILTSYLITLSITRPLAEGIRIADALADGNLTIQVSST
GRDETGLLIEHLGHMVHNLRDMISRTVDISSGIAAASNQLHSTATQIATGAEEVSCQANTVATASEEMSATSSDIARNCS
MAADASRQTSESANSGAQVVNETITGMNVIADRVRETSKTIEALGTRSDQIGEIIGTIEDIADQTNLLALNAAIEAARAG
EQGRGFAVVADEVRALAERTTKATREIGEMIKAIQRETQEAVTAMDEGVREVEKGAESSQKSGRALEEILERINEVSMQV
NQIATAAEEQTATTGEVTSNIQQINEVVHSTAQGAEETAKAAAQLAGQAEELQGLVSRFRLS
>Mature_541_residues
STLLNMKIGARLGFGFGFVLLLLVAMGGISIRNMGAINRGVEQIVTVDYAKINLANDINARVVTLLENFALMQLKDQTGK
IEVKRDIDTLRSEYRGLMEKLEKLETTAEGKGLIAAAKVALANGTTANNRVIELSLANSAAEALSVYNNEAYPLNRKIKA
AFNDIVKYQEGVMEVSHGEATRVFGSTRLINVVLVVVALLVGILTSYLITLSITRPLAEGIRIADALADGNLTIQVSSTG
RDETGLLIEHLGHMVHNLRDMISRTVDISSGIAAASNQLHSTATQIATGAEEVSCQANTVATASEEMSATSSDIARNCSM
AADASRQTSESANSGAQVVNETITGMNVIADRVRETSKTIEALGTRSDQIGEIIGTIEDIADQTNLLALNAAIEAARAGE
QGRGFAVVADEVRALAERTTKATREIGEMIKAIQRETQEAVTAMDEGVREVEKGAESSQKSGRALEEILERINEVSMQVN
QIATAAEEQTATTGEVTSNIQQINEVVHSTAQGAEETAKAAAQLAGQAEELQGLVSRFRLS

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=392, Percent_Identity=30.6122448979592, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788194, Length=373, Percent_Identity=31.9034852546917, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI2367378, Length=310, Percent_Identity=34.8387096774194, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1788195, Length=335, Percent_Identity=32.8358208955224, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789453, Length=353, Percent_Identity=28.8951841359773, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 57743; Mature: 57612

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLLNMKIGARLGFGFGFVLLLLVAMGGISIRNMGAINRGVEQIVTVDYAKINLANDIN
CCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHEEEECCCCH
ARVVTLLENFALMQLKDQTGKIEVKRDIDTLRSEYRGLMEKLEKLETTAEGKGLIAAAKV
HHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALANGTTANNRVIELSLANSAAEALSVYNNEAYPLNRKIKAAFNDIVKYQEGVMEVSHGE
HHCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ATRVFGSTRLINVVLVVVALLVGILTSYLITLSITRPLAEGIRIADALADGNLTIQVSST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
GRDETGLLIEHLGHMVHNLRDMISRTVDISSGIAAASNQLHSTATQIATGAEEVSCQANT
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCH
VATASEEMSATSSDIARNCSMAADASRQTSESANSGAQVVNETITGMNVIADRVRETSKT
HHCCHHHHHHHHHHHHHHCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEALGTRSDQIGEIIGTIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRALAERT
HHHHCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
TKATREIGEMIKAIQRETQEAVTAMDEGVREVEKGAESSQKSGRALEEILERINEVSMQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHH
NQIATAAEEQTATTGEVTSNIQQINEVVHSTAQGAEETAKAAAQLAGQAEELQGLVSRFR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHC
LS
CC
>Mature Secondary Structure 
STLLNMKIGARLGFGFGFVLLLLVAMGGISIRNMGAINRGVEQIVTVDYAKINLANDIN
CCEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHEEEECCCCH
ARVVTLLENFALMQLKDQTGKIEVKRDIDTLRSEYRGLMEKLEKLETTAEGKGLIAAAKV
HHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALANGTTANNRVIELSLANSAAEALSVYNNEAYPLNRKIKAAFNDIVKYQEGVMEVSHGE
HHCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ATRVFGSTRLINVVLVVVALLVGILTSYLITLSITRPLAEGIRIADALADGNLTIQVSST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
GRDETGLLIEHLGHMVHNLRDMISRTVDISSGIAAASNQLHSTATQIATGAEEVSCQANT
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCH
VATASEEMSATSSDIARNCSMAADASRQTSESANSGAQVVNETITGMNVIADRVRETSKT
HHCCHHHHHHHHHHHHHHCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEALGTRSDQIGEIIGTIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRALAERT
HHHHCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
TKATREIGEMIKAIQRETQEAVTAMDEGVREVEKGAESSQKSGRALEEILERINEVSMQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHH
NQIATAAEEQTATTGEVTSNIQQINEVVHSTAQGAEETAKAAAQLAGQAEELQGLVSRFR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHC
LS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]